CA2233309A1 - Recombinant hepatitis c virus rna replicase - Google Patents

Recombinant hepatitis c virus rna replicase Download PDF

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Publication number
CA2233309A1
CA2233309A1 CA002233309A CA2233309A CA2233309A1 CA 2233309 A1 CA2233309 A1 CA 2233309A1 CA 002233309 A CA002233309 A CA 002233309A CA 2233309 A CA2233309 A CA 2233309A CA 2233309 A1 CA2233309 A1 CA 2233309A1
Authority
CA
Canada
Prior art keywords
rdrp
hcv
ala
leu
rna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002233309A
Other languages
English (en)
French (fr)
Inventor
Curt H. Hagedorn
Reinoldus H. Al
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Emory University
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2233309A1 publication Critical patent/CA2233309A1/en
Abandoned legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/127RNA-directed RNA polymerase (2.7.7.48), i.e. RNA replicase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA002233309A 1995-09-27 1996-09-27 Recombinant hepatitis c virus rna replicase Abandoned CA2233309A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US438395P 1995-09-27 1995-09-27
US60/004,383 1995-09-27

Publications (1)

Publication Number Publication Date
CA2233309A1 true CA2233309A1 (en) 1997-04-03

Family

ID=21710540

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002233309A Abandoned CA2233309A1 (en) 1995-09-27 1996-09-27 Recombinant hepatitis c virus rna replicase

Country Status (8)

Country Link
US (2) US5981247A (enExample)
EP (1) EP0859833B1 (enExample)
JP (1) JP4191797B2 (enExample)
AT (1) ATE318896T1 (enExample)
AU (1) AU719122B2 (enExample)
CA (1) CA2233309A1 (enExample)
DE (1) DE69635864T2 (enExample)
WO (1) WO1997012033A1 (enExample)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1278077B1 (it) 1995-05-25 1997-11-17 Angeletti P Ist Richerche Bio Metodologia per riprodurre in vitro le attivita' di rna polimerasi rna-dipendente e di nucleotidiltransferasi terminale codificate dal
FR2761695B1 (fr) * 1997-04-04 1999-09-24 Bio Merieux Arn polymerase fonctionnant preferentiellement sur matrice d'arn, sa preparation et son utilisation
CA2312484A1 (en) * 1997-12-11 1999-06-17 Smithkline Beecham Corporation Hepatitis c virus ns5b truncated protein and methods thereof to identify antiviral compounds
CA2322008C (en) 1998-02-25 2011-06-28 Emory University 2'-fluoronucleosides
EP1068362A4 (en) * 1998-04-02 2005-07-20 Viropharma Inc HEPATITIS C VIRUS NS5B COMPOSITIONS AND METHOD FOR THEIR USE
WO1999067396A1 (en) * 1998-06-24 1999-12-29 International Reagents Corporation Hcv-derived rna polymerase gene
WO2000033635A2 (en) * 1998-12-10 2000-06-15 Eli Lilly And Company Primer-independent rna synthesis catalyzed by hcv polymerase
EP1600452A3 (en) 1999-11-12 2008-09-10 Pharmasset, Inc. Synthesis of 2'-deoxy-L-nucleosides
AU764095B2 (en) * 2000-04-07 2003-08-07 Shionogi & Co., Ltd. Preincubation assay method
MY164523A (en) 2000-05-23 2017-12-29 Univ Degli Studi Cagliari Methods and compositions for treating hepatitis c virus
IL153020A0 (en) 2000-05-26 2003-06-24 Idenix Cayman Ltd Methods and compositions for treating flaviviruses and pestiviruses
GB0029539D0 (en) * 2000-12-04 2001-01-17 Isis Innovation Method for identifying modulatorss of transcription
CA2437072A1 (en) * 2001-01-31 2002-08-08 Bristol-Myers Squibb Pharma Company In vitro system for replication of rna-dependent rna polymerase (rdrp) viruses
WO2002095002A2 (en) * 2001-05-22 2002-11-28 University Of Chicago N4 virion single-stranded dna dependent rna polymerase
US20030170810A1 (en) * 2001-09-18 2003-09-11 Masoud Vedadi Methods and apparatuses for purification
US20030104445A1 (en) * 2001-09-25 2003-06-05 Hayes Robert J. RNA dependent RNA polymerase mediated protein evolution
JP2005504087A (ja) 2001-09-28 2005-02-10 イデニクス(ケイマン)リミテツド 4’が修飾されたヌクレオシドを使用するc型肝炎ウイルス治療のための方法および組成物
KR100447578B1 (ko) * 2001-11-01 2004-09-07 주식회사 이매진 Hcv 자가 복제 효소 활성의 세포내 측정용 키트 및측정 방법
AU2002360394A1 (en) * 2001-11-16 2003-06-10 University Of Massachusetts Facilitation of rna interference
US7608600B2 (en) 2002-06-28 2009-10-27 Idenix Pharmaceuticals, Inc. Modified 2′ and 3′-nucleoside prodrugs for treating Flaviviridae infections
EP1523489B1 (en) 2002-06-28 2014-03-12 IDENIX Pharmaceuticals, Inc. Modified 2' and 3' -nucleoside produgs for treating flaviridae infections
KR20050088079A (ko) 2002-11-15 2005-09-01 이데닉스 (케이만) 리미티드 2'-분지형 뉴클레오시드 및 플라비비리다에 돌연변이
ES2906207T3 (es) 2003-05-30 2022-04-13 Gilead Pharmasset Llc Análogos de nucleósidos fluorados modificados
JP2007501185A (ja) 2003-07-25 2007-01-25 イデニクス(ケイマン)リミテツド C型肝炎を含むフラビウイルス関連疾患を治療するためのプリンヌクレオシド類似体
WO2005070957A1 (en) * 2004-01-09 2005-08-04 Merck & Co., Inc. Hcv rna-dependent rna polymerase
US7414031B2 (en) 2004-11-22 2008-08-19 Genelabs Technologies, Inc. 5-nitro-nucleoside compounds for treating viral infections
EP1853617A1 (en) 2005-02-28 2007-11-14 Genelabs Technologies, Inc. Tricyclic-nucleoside prodrugs for treating viral infections
US7405204B2 (en) 2005-04-25 2008-07-29 Genelabs Technologies, Inc. Nucleoside compounds for treating viral infections
US7964580B2 (en) 2007-03-30 2011-06-21 Pharmasset, Inc. Nucleoside phosphoramidate prodrugs
US20090318380A1 (en) 2007-11-20 2009-12-24 Pharmasset, Inc. 2',4'-substituted nucleosides as antiviral agents
CA2729168A1 (en) 2008-07-02 2010-02-04 Idenix Pharmaceuticals, Inc. Compounds and pharmaceutical compositions for the treatment of viral infections
EP2166016A1 (en) 2008-09-18 2010-03-24 Centocor Ortho Biotech Products L.P. Phosphoramidate Derivatives of Nucleosides
BRPI0922508A8 (pt) 2008-12-23 2016-01-19 Pharmasset Inc Análogos de nucleosídeo
NZ593648A (en) 2008-12-23 2013-09-27 Gilead Pharmasset Llc Nucleoside phosphoramidates
EP2376515A1 (en) 2008-12-23 2011-10-19 Pharmasset, Inc. Synthesis of purine nucleosides
TWI598358B (zh) 2009-05-20 2017-09-11 基利法瑪席特有限責任公司 核苷磷醯胺
US8618076B2 (en) 2009-05-20 2013-12-31 Gilead Pharmasset Llc Nucleoside phosphoramidates
WO2011023801A1 (en) 2009-08-31 2011-03-03 F. Hoffmann-La Roche Ag Hcv ns3/4a replicon shuttle vectors
JP2013511030A (ja) 2009-11-14 2013-03-28 エフ.ホフマン−ラ ロシュ アーゲー Hcv治療の迅速な反応を予測するためのバイオマーカー
RU2012127201A (ru) 2009-12-02 2014-01-20 Ф.Хоффманн-Ля Рош Аг Биомаркеры для прогнозирования устойчивого ответа на лечение вируса гепатита с
CN102858790A (zh) 2010-03-31 2013-01-02 吉利德制药有限责任公司 核苷氨基磷酸酯
WO2011123672A1 (en) 2010-03-31 2011-10-06 Pharmasset, Inc. Purine nucleoside phosphoramidate
US20110245328A1 (en) 2010-03-31 2011-10-06 Hyunsoon Kang HCV NS5A Replicon Shuttle Vectors
MX2012011222A (es) 2010-04-01 2013-01-18 Centre Nat Rech Scient Compuestos y composiciones farmaceuticas para el tratamiento de infecciones virales.
AR082453A1 (es) 2010-04-21 2012-12-12 Novartis Ag Compuestos de furopiridina, composiciones farmaceuticas que los contienen y usos de los mismos
CN103025728B (zh) 2010-07-22 2015-11-25 诺华股份有限公司 2,3,5-三取代的噻吩化合物及其用途
PT3042910T (pt) 2010-11-30 2019-04-16 Gilead Pharmasset Llc 2'-espiro-nucleósidos para utilização na terapia da hepatite c
US9351989B2 (en) 2010-12-29 2016-05-31 Inhibitex, Inc. Substituted purine nucleosides, phosphoroamidate and phosphorodiamidate derivatives for treatment of viral infections
CN103842369A (zh) 2011-03-31 2014-06-04 埃迪尼克斯医药公司 用于治疗病毒感染的化合物和药物组合物
WO2013039920A1 (en) 2011-09-12 2013-03-21 Idenix Pharmaceuticals, Inc. Substituted carbonyloxymethylphosphoramidate compounds and pharmaceutical compositions for the treatment of viral infections
US8951985B2 (en) 2011-09-12 2015-02-10 Idenix Pharmaceuticals, Inc. Compounds and pharmaceutical compositions for the treatment of viral infections
WO2013056046A1 (en) 2011-10-14 2013-04-18 Idenix Pharmaceuticals, Inc. Substituted 3',5'-cyclic phosphates of purine nucleotide compounds and pharmaceutical compositions for the treatment of viral infections
US8889159B2 (en) 2011-11-29 2014-11-18 Gilead Pharmasset Llc Compositions and methods for treating hepatitis C virus
US20150064253A1 (en) 2013-08-27 2015-03-05 Gilead Pharmasset Llc Combination formulation of two antiviral compounds

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5350671A (en) * 1987-11-18 1994-09-27 Chiron Corporation HCV immunoassays employing C domain antigens
US5683864A (en) * 1987-11-18 1997-11-04 Chiron Corporation Combinations of hepatitis C virus (HCV) antigens for use in immunoassays for anti-HCV antibodies
US5726155A (en) * 1993-08-02 1998-03-10 The Scripps Research Institute Regulation of oxidative burst using LMWG-derived peptides and analogs
IT1278077B1 (it) * 1995-05-25 1997-11-17 Angeletti P Ist Richerche Bio Metodologia per riprodurre in vitro le attivita' di rna polimerasi rna-dipendente e di nucleotidiltransferasi terminale codificate dal

Also Published As

Publication number Publication date
EP0859833A1 (en) 1998-08-26
DE69635864D1 (de) 2006-04-27
EP0859833B1 (en) 2006-03-01
JPH11514862A (ja) 1999-12-21
US6248589B1 (en) 2001-06-19
AU7200796A (en) 1997-04-17
WO1997012033A1 (en) 1997-04-03
ATE318896T1 (de) 2006-03-15
US5981247A (en) 1999-11-09
AU719122B2 (en) 2000-05-04
EP0859833A4 (en) 2001-03-28
DE69635864T2 (de) 2006-10-26
JP4191797B2 (ja) 2008-12-03

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Legal Events

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EEER Examination request
FZDE Discontinued