CA2228769C - Method of purifying, stabilising or isolating nucleic acids contained in biological matter - Google Patents
Method of purifying, stabilising or isolating nucleic acids contained in biological matter Download PDFInfo
- Publication number
- CA2228769C CA2228769C CA2228769A CA2228769A CA2228769C CA 2228769 C CA2228769 C CA 2228769C CA 2228769 A CA2228769 A CA 2228769A CA 2228769 A CA2228769 A CA 2228769A CA 2228769 C CA2228769 C CA 2228769C
- Authority
- CA
- Canada
- Prior art keywords
- nucleic acids
- nucleic
- adsorption matrix
- acid
- reagent kit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 100
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 100
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 100
- 238000000034 method Methods 0.000 title claims abstract description 63
- 230000003019 stabilising effect Effects 0.000 title 1
- 239000011159 matrix material Substances 0.000 claims abstract description 53
- 238000001179 sorption measurement Methods 0.000 claims abstract description 52
- 239000000356 contaminant Substances 0.000 claims abstract description 29
- 230000000087 stabilizing effect Effects 0.000 claims abstract description 8
- 239000000126 substance Substances 0.000 claims description 20
- 239000003153 chemical reaction reagent Substances 0.000 claims description 19
- 206010028980 Neoplasm Diseases 0.000 claims description 18
- 235000014633 carbohydrates Nutrition 0.000 claims description 17
- 150000001720 carbohydrates Chemical class 0.000 claims description 16
- 238000006911 enzymatic reaction Methods 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 16
- 238000004458 analytical method Methods 0.000 claims description 15
- 230000003321 amplification Effects 0.000 claims description 14
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 14
- 238000003752 polymerase chain reaction Methods 0.000 claims description 14
- 229920001592 potato starch Polymers 0.000 claims description 13
- 241000196324 Embryophyta Species 0.000 claims description 12
- 241001465754 Metazoa Species 0.000 claims description 12
- 239000000872 buffer Substances 0.000 claims description 12
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 11
- 235000013312 flour Nutrition 0.000 claims description 11
- 239000001913 cellulose Substances 0.000 claims description 10
- 229920002678 cellulose Polymers 0.000 claims description 10
- 239000007857 degradation product Substances 0.000 claims description 8
- -1 polyethylene Polymers 0.000 claims description 8
- 238000001514 detection method Methods 0.000 claims description 7
- 210000003608 fece Anatomy 0.000 claims description 7
- 239000004698 Polyethylene Substances 0.000 claims description 6
- 238000003745 diagnosis Methods 0.000 claims description 6
- 238000002955 isolation Methods 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 6
- 229920000573 polyethylene Polymers 0.000 claims description 6
- 210000004369 blood Anatomy 0.000 claims description 5
- 239000008280 blood Substances 0.000 claims description 5
- 239000007853 buffer solution Substances 0.000 claims description 5
- 230000002255 enzymatic effect Effects 0.000 claims description 5
- 210000001035 gastrointestinal tract Anatomy 0.000 claims description 5
- 238000000746 purification Methods 0.000 claims description 5
- 150000003839 salts Chemical class 0.000 claims description 5
- 230000009946 DNA mutation Effects 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 241000233866 Fungi Species 0.000 claims description 4
- 229920002472 Starch Polymers 0.000 claims description 4
- 108700025716 Tumor Suppressor Genes Proteins 0.000 claims description 4
- 102000044209 Tumor Suppressor Genes Human genes 0.000 claims description 4
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 230000000035 biogenic effect Effects 0.000 claims description 4
- 210000001124 body fluid Anatomy 0.000 claims description 4
- 239000010839 body fluid Substances 0.000 claims description 4
- 239000000284 extract Substances 0.000 claims description 4
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 4
- 238000007834 ligase chain reaction Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 239000011707 mineral Substances 0.000 claims description 4
- 239000010801 sewage sludge Substances 0.000 claims description 4
- 239000002689 soil Substances 0.000 claims description 4
- 239000008107 starch Substances 0.000 claims description 4
- 235000019698 starch Nutrition 0.000 claims description 4
- 239000002351 wastewater Substances 0.000 claims description 4
- 102000001554 Hemoglobins Human genes 0.000 claims description 3
- 108010054147 Hemoglobins Proteins 0.000 claims description 3
- 208000005016 Intestinal Neoplasms Diseases 0.000 claims description 3
- 108091092878 Microsatellite Proteins 0.000 claims description 3
- 108700020796 Oncogene Proteins 0.000 claims description 3
- 102000043276 Oncogene Human genes 0.000 claims description 3
- 239000003613 bile acid Substances 0.000 claims description 3
- 239000003833 bile salt Substances 0.000 claims description 3
- 210000000988 bone and bone Anatomy 0.000 claims description 3
- 229920000126 latex Polymers 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 244000005700 microbiome Species 0.000 claims description 3
- 239000010453 quartz Substances 0.000 claims description 3
- 239000000377 silicon dioxide Substances 0.000 claims description 3
- 230000006641 stabilisation Effects 0.000 claims description 3
- 238000011105 stabilization Methods 0.000 claims description 3
- 229920001059 synthetic polymer Polymers 0.000 claims description 3
- 230000003612 virological effect Effects 0.000 claims description 3
- 244000068988 Glycine max Species 0.000 claims description 2
- 235000010469 Glycine max Nutrition 0.000 claims description 2
- 229920002527 Glycogen Polymers 0.000 claims description 2
- 239000002033 PVDF binder Substances 0.000 claims description 2
- 206010061902 Pancreatic neoplasm Diseases 0.000 claims description 2
- 240000004713 Pisum sativum Species 0.000 claims description 2
- 235000010582 Pisum sativum Nutrition 0.000 claims description 2
- 239000004743 Polypropylene Substances 0.000 claims description 2
- 244000061456 Solanum tuberosum Species 0.000 claims description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 2
- 240000008042 Zea mays Species 0.000 claims description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 2
- 235000013339 cereals Nutrition 0.000 claims description 2
- 238000003776 cleavage reaction Methods 0.000 claims description 2
- 239000004744 fabric Substances 0.000 claims description 2
- 239000000835 fiber Substances 0.000 claims description 2
- 239000000499 gel Substances 0.000 claims description 2
- 229940096919 glycogen Drugs 0.000 claims description 2
- 230000005484 gravity Effects 0.000 claims description 2
- 239000004816 latex Substances 0.000 claims description 2
- 235000009973 maize Nutrition 0.000 claims description 2
- 229910044991 metal oxide Inorganic materials 0.000 claims description 2
- 150000004706 metal oxides Chemical class 0.000 claims description 2
- 229910003455 mixed metal oxide Inorganic materials 0.000 claims description 2
- 201000002528 pancreatic cancer Diseases 0.000 claims description 2
- 229920001155 polypropylene Polymers 0.000 claims description 2
- 229920002981 polyvinylidene fluoride Polymers 0.000 claims description 2
- 239000011148 porous material Substances 0.000 claims description 2
- 235000012015 potatoes Nutrition 0.000 claims description 2
- 230000010076 replication Effects 0.000 claims description 2
- 230000007017 scission Effects 0.000 claims description 2
- 230000002459 sustained effect Effects 0.000 claims description 2
- 239000010457 zeolite Substances 0.000 claims description 2
- 208000035143 Bacterial infection Diseases 0.000 claims 1
- 208000036142 Viral infection Diseases 0.000 claims 1
- 208000022362 bacterial infectious disease Diseases 0.000 claims 1
- 125000000837 carbohydrate group Chemical group 0.000 claims 1
- 239000010410 layer Substances 0.000 claims 1
- 230000000813 microbial effect Effects 0.000 claims 1
- 230000009385 viral infection Effects 0.000 claims 1
- 108020004414 DNA Proteins 0.000 description 21
- 230000002550 fecal effect Effects 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 5
- 229940098773 bovine serum albumin Drugs 0.000 description 5
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- PNEYBMLMFCGWSK-UHFFFAOYSA-N Alumina Chemical compound [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 239000013611 chromosomal DNA Substances 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 108010067770 Endopeptidase K Proteins 0.000 description 2
- 108091027305 Heteroduplex Proteins 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 108091093105 Nuclear DNA Proteins 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- 239000012752 auxiliary agent Substances 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 201000009019 intestinal benign neoplasm Diseases 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 210000004379 membrane Anatomy 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 230000000869 mutational effect Effects 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 230000003252 repetitive effect Effects 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 238000003260 vortexing Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 208000003200 Adenoma Diseases 0.000 description 1
- 206010001233 Adenoma benign Diseases 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- 208000001333 Colorectal Neoplasms Diseases 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 206010061309 Neoplasm progression Diseases 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 239000012494 Quartz wool Substances 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- MCMNRKCIXSYSNV-UHFFFAOYSA-N ZrO2 Inorganic materials O=[Zr]=O MCMNRKCIXSYSNV-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 238000009534 blood test Methods 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 229910010293 ceramic material Inorganic materials 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 208000029664 classic familial adenomatous polyposis Diseases 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 235000006694 eating habits Nutrition 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000004374 forensic analysis Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 239000011491 glass wool Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- ZRALSGWEFCBTJO-UHFFFAOYSA-O guanidinium Chemical compound NC(N)=[NH2+] ZRALSGWEFCBTJO-UHFFFAOYSA-O 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 238000007857 nested PCR Methods 0.000 description 1
- RVTZCBVAJQQJTK-UHFFFAOYSA-N oxygen(2-);zirconium(4+) Chemical compound [O-2].[O-2].[Zr+4] RVTZCBVAJQQJTK-UHFFFAOYSA-N 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 108700042226 ras Genes Proteins 0.000 description 1
- 238000009666 routine test Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 239000004408 titanium dioxide Substances 0.000 description 1
- 230000005751 tumor progression Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 244000052613 viral pathogen Species 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Detergent Compositions (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Processing Of Solid Wastes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19530132.3 | 1995-08-16 | ||
| DE19530132A DE19530132C2 (de) | 1995-08-16 | 1995-08-16 | Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien |
| PCT/EP1996/003595 WO1997007239A1 (de) | 1995-08-16 | 1996-08-14 | Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2228769A1 CA2228769A1 (en) | 1997-02-27 |
| CA2228769C true CA2228769C (en) | 2011-04-12 |
Family
ID=7769624
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2228769A Expired - Lifetime CA2228769C (en) | 1995-08-16 | 1996-08-14 | Method of purifying, stabilising or isolating nucleic acids contained in biological matter |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US6084091A (enExample) |
| EP (1) | EP0851937B1 (enExample) |
| JP (1) | JP4264133B2 (enExample) |
| AT (1) | ATE215611T1 (enExample) |
| AU (1) | AU712331B2 (enExample) |
| CA (1) | CA2228769C (enExample) |
| DE (2) | DE19530132C2 (enExample) |
| DK (1) | DK0851937T3 (enExample) |
| WO (1) | WO1997007239A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7981612B2 (en) | 1999-12-07 | 2011-07-19 | Mayo Foundation For Medical Education And Research | Methods of screening for supracolonic neoplasms based on stool samples containing a nucleic acid marker indicative of a neoplasm |
Families Citing this family (63)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6448002B1 (en) * | 1996-08-26 | 2002-09-10 | Invitek Gmbh | Method to detect clinically relevant mutations of the DNA sequence of ki-ras oncogene, its use and a testkit for early diagnosis of tumors |
| WO1998058081A1 (en) * | 1997-06-16 | 1998-12-23 | Exact Laboratories, Inc. | Methods for stool sample preparation |
| US6268136B1 (en) * | 1997-06-16 | 2001-07-31 | Exact Science Corporation | Methods for stool sample preparation |
| US6406857B1 (en) | 1997-06-16 | 2002-06-18 | Exact Sciences Corporation | Methods for stool sample preparation |
| US6241980B1 (en) | 1997-11-04 | 2001-06-05 | Becton, Dickinson And Company | Sample processing method using ion exchange resin |
| US7569342B2 (en) | 1997-12-10 | 2009-08-04 | Sierra Molecular Corp. | Removal of molecular assay interferences |
| EP0939118A1 (en) * | 1998-02-20 | 1999-09-01 | Universiteit Maastricht | Method for isolating DNA and RNA from faeces |
| MXPA00009754A (es) | 1998-04-08 | 2002-05-08 | Metabolix Inc | Metodos para la separacion y purificacion de biopolimeros. |
| EP1086164B1 (en) | 1998-06-09 | 2006-08-16 | Metabolix, Inc. | Methods and apparatus for the production of amorphous polymer suspensions |
| DE19900638C2 (de) * | 1999-01-11 | 2002-12-19 | Max Planck Gesellschaft | Methode zur Isolierung von DNA aus biologischen Materialien |
| WO2000050640A1 (en) * | 1999-02-25 | 2000-08-31 | Exact Laboratories, Inc. | Methods for preserving dna integrity |
| JP4794052B2 (ja) * | 1999-04-09 | 2011-10-12 | ジェンザイム・コーポレーション | 癌の指標である核酸を検出するための方法 |
| US6849403B1 (en) | 1999-09-08 | 2005-02-01 | Exact Sciences Corporation | Apparatus and method for drug screening |
| US6586177B1 (en) | 1999-09-08 | 2003-07-01 | Exact Sciences Corporation | Methods for disease detection |
| US6383783B1 (en) | 1999-09-21 | 2002-05-07 | 3M Innovative Properties Company | Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant |
| US6919174B1 (en) * | 1999-12-07 | 2005-07-19 | Exact Sciences Corporation | Methods for disease detection |
| EP1252338B1 (de) * | 2000-02-04 | 2007-10-17 | QIAGEN GmbH | Nukleinsäure-isolierung aus stuhlproben und anderen biologischen materialien, die reich an inhibitoren sind |
| WO2001062976A1 (en) * | 2000-02-23 | 2001-08-30 | Wen Shao | Rapid nucleic acid separation, isolation and purification methods |
| GB0015777D0 (en) * | 2000-06-28 | 2000-08-16 | Tayside University Hospitals N | DNA Extraction |
| EP1442045B1 (en) * | 2001-11-06 | 2009-01-07 | Cortex Biochem Inc. | Isolation and purification of nucleic acids |
| GB0130821D0 (en) * | 2001-12-24 | 2002-02-06 | Tayside University Hospitals N | DNA screening |
| AU2003213107A1 (en) | 2002-02-15 | 2003-09-09 | Exact Sciences Corporation | Methods for analysis of molecular events |
| US7482116B2 (en) | 2002-06-07 | 2009-01-27 | Dna Genotek Inc. | Compositions and methods for obtaining nucleic acids from sputum |
| US20040259101A1 (en) * | 2003-06-20 | 2004-12-23 | Shuber Anthony P. | Methods for disease screening |
| WO2005026347A1 (en) * | 2003-09-09 | 2005-03-24 | Fuji Photo Film Co., Ltd. | Method for isolating and purifying a nucleic acid |
| KR20060081714A (ko) * | 2003-10-02 | 2006-07-13 | 미시시피 주립대학 | 폐수 처리 플랜트 슬러지로부터의 바이오-디젤 및 다른귀중한 화학물질의 제조방법 |
| US20050130177A1 (en) * | 2003-12-12 | 2005-06-16 | 3M Innovative Properties Company | Variable valve apparatus and methods |
| US7939249B2 (en) | 2003-12-24 | 2011-05-10 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using a microfluidic device and concentration step |
| US7727710B2 (en) * | 2003-12-24 | 2010-06-01 | 3M Innovative Properties Company | Materials, methods, and kits for reducing nonspecific binding of molecules to a surface |
| US20050142571A1 (en) * | 2003-12-24 | 2005-06-30 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using solid phase material |
| EP1709197A4 (en) * | 2003-12-30 | 2007-07-04 | Sigma Aldrich Co | RAPID PREPARATION OF NUCLEIC ACIDS BY ENZYMATIC DEPENDENCE |
| US8426126B2 (en) * | 2004-03-18 | 2013-04-23 | Applied Biosystems, Llc | Modified surfaces as solid supports for nucleic acid purification |
| WO2005111244A2 (en) * | 2004-05-10 | 2005-11-24 | Exact Sciences Corporation | Methods for detecting a mutant nucleic acid |
| WO2006026654A2 (en) * | 2004-08-27 | 2006-03-09 | Exact Sciences Corporation | Method for detecting a recombinant event |
| US7195878B2 (en) * | 2004-09-30 | 2007-03-27 | Cryo-Genomics | Device and method for fecal testing |
| WO2006047787A2 (en) | 2004-10-27 | 2006-05-04 | Exact Sciences Corporation | Method for monitoring disease progression or recurrence |
| US9777314B2 (en) * | 2005-04-21 | 2017-10-03 | Esoterix Genetic Laboratories, Llc | Analysis of heterogeneous nucleic acid samples |
| JP4819427B2 (ja) | 2005-07-15 | 2011-11-24 | 株式会社リコー | 画像形成装置、画像形成方法、及びプロセスカートリッジ |
| JP4590344B2 (ja) | 2005-11-21 | 2010-12-01 | 株式会社リコー | 静電潜像担持体及びそれを用いた画像形成装置、プロセスカートリッジ及び画像形成方法 |
| JP2009011247A (ja) * | 2007-07-05 | 2009-01-22 | Sumitomo Bakelite Co Ltd | 遺伝子の検出方法 |
| US10125388B2 (en) | 2007-10-31 | 2018-11-13 | Akonni Biosystems, Inc. | Integrated sample processing system |
| US20090111193A1 (en) | 2007-10-31 | 2009-04-30 | Cooney Christopher G | Sample preparation device |
| US9428746B2 (en) | 2007-10-31 | 2016-08-30 | Akonni Biosystems, Inc. | Method and kit for purifying nucleic acids |
| US7759112B2 (en) * | 2007-10-31 | 2010-07-20 | Akonni Biosystems, Inc. | Apparatus, system, and method for purifying nucleic acids |
| CA2703884C (en) * | 2007-11-05 | 2015-03-17 | Eiken Kagaku Kabushiki Kaisha | Method and kit for preparation of sample for use in nucleic acid amplification |
| JP5599013B2 (ja) * | 2008-04-03 | 2014-10-01 | キヤノン株式会社 | 血液検体からの微生物核酸の抽出方法 |
| WO2010010914A1 (ja) * | 2008-07-23 | 2010-01-28 | オリンパス株式会社 | 糞便試料からの核酸回収方法、核酸解析方法及び糞便試料処理装置 |
| WO2010028382A2 (en) * | 2008-09-05 | 2010-03-11 | Mayo Foundation For Medical Education And Research | Collecting and processing complex macromolecular mixtures |
| WO2010134245A1 (ja) * | 2009-05-20 | 2010-11-25 | オリンパス株式会社 | 哺乳細胞由来核酸の回収方法、核酸解析方法、及び採便用キット |
| US8039613B2 (en) | 2009-08-28 | 2011-10-18 | Promega Corporation | Methods of purifying a nucleic acid and formulation and kit for use in performing such methods |
| US8222397B2 (en) * | 2009-08-28 | 2012-07-17 | Promega Corporation | Methods of optimal purification of nucleic acids and kit for use in performing such methods |
| KR101590544B1 (ko) | 2010-01-07 | 2016-02-18 | 빅텍 프라이빗 리미티드 | 핵산 분리법 및 그를 위한 키트 |
| JP5743701B2 (ja) * | 2011-05-10 | 2015-07-01 | 英二 小西 | Ｒｎａ検出用試薬およびｒｎａ検出方法 |
| JP5743704B2 (ja) * | 2011-05-12 | 2015-07-01 | 住友大阪セメント株式会社 | Ｒｎａ検出用試薬およびｒｎａ検出方法 |
| BR112013032629A2 (pt) | 2011-06-19 | 2017-08-01 | Abogen Inc | dispositivos, soluções e métodos para coletamento de amostra |
| WO2013035062A1 (en) * | 2011-09-06 | 2013-03-14 | North-West University | A method of preparing biological material |
| WO2013067509A1 (en) * | 2011-11-04 | 2013-05-10 | Bio-Rad Laboratories, Inc. | Simultaneous purification of cell components |
| US9766207B2 (en) | 2011-11-04 | 2017-09-19 | Bio-Rad Laboratories, Inc. | Affinity methods and compositions employing electronic control of pH |
| US9658195B2 (en) | 2012-02-15 | 2017-05-23 | Bio-Rad Laboratories, Inc. | Electronic control of pH and ionic strength |
| US9321012B2 (en) | 2012-04-04 | 2016-04-26 | Bio-Rad Laboratories, Inc. | Electronic protein fractionation |
| DK3114225T3 (da) | 2014-03-07 | 2021-07-26 | Dna Genotek Inc | Sammensætning og fremgangsmåde til stabilisering af nukleinsyrer i biologiske prøver |
| HK1256342A1 (zh) | 2015-09-10 | 2019-09-20 | Life Technologies Corporation | 环境或生物样本中的核酸纯化 |
| CN113832141B (zh) * | 2021-09-30 | 2024-03-08 | 苏州唯善生物科技有限公司 | 粪便人源基因组dna特异性纯化方法及试剂盒 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0189280A3 (en) * | 1985-01-23 | 1987-01-07 | Dekalb-Pfizer Genetics | Qualitative testing for nucleic acid |
| NL8900725A (nl) * | 1989-03-23 | 1990-10-16 | Az Univ Amsterdam | Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur. |
| US5334499A (en) * | 1989-04-17 | 1994-08-02 | Eastman Kodak Company | Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction |
| JP3633932B2 (ja) * | 1992-04-01 | 2005-03-30 | ザ ジョーンズ ホプキンズ ユニバーシティー スクール オブ メディシン | 糞便試料から単離した哺乳類の核酸を検出する方法、およびその検出用試薬 |
| WO1993025711A1 (en) * | 1992-06-12 | 1993-12-23 | Gen-Probe Incorporated | Preparation of nucleic acid from blood |
-
1995
- 1995-08-16 DE DE19530132A patent/DE19530132C2/de not_active Expired - Lifetime
-
1996
- 1996-08-14 AT AT96928466T patent/ATE215611T1/de active
- 1996-08-14 DE DE59609027T patent/DE59609027D1/de not_active Expired - Lifetime
- 1996-08-14 EP EP96928466A patent/EP0851937B1/de not_active Expired - Lifetime
- 1996-08-14 WO PCT/EP1996/003595 patent/WO1997007239A1/de not_active Ceased
- 1996-08-14 CA CA2228769A patent/CA2228769C/en not_active Expired - Lifetime
- 1996-08-14 JP JP50894597A patent/JP4264133B2/ja not_active Expired - Lifetime
- 1996-08-14 US US09/011,567 patent/US6084091A/en not_active Expired - Lifetime
- 1996-08-14 DK DK96928466T patent/DK0851937T3/da active
- 1996-08-14 AU AU68216/96A patent/AU712331B2/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7981612B2 (en) | 1999-12-07 | 2011-07-19 | Mayo Foundation For Medical Education And Research | Methods of screening for supracolonic neoplasms based on stool samples containing a nucleic acid marker indicative of a neoplasm |
Also Published As
| Publication number | Publication date |
|---|---|
| DE19530132C2 (de) | 1998-07-16 |
| DE19530132A1 (de) | 1997-02-20 |
| JPH11511020A (ja) | 1999-09-28 |
| AU712331B2 (en) | 1999-11-04 |
| ATE215611T1 (de) | 2002-04-15 |
| EP0851937B1 (de) | 2002-04-03 |
| WO1997007239A1 (de) | 1997-02-27 |
| DE59609027D1 (de) | 2002-05-08 |
| JP4264133B2 (ja) | 2009-05-13 |
| US6084091A (en) | 2000-07-04 |
| DK0851937T3 (da) | 2002-06-24 |
| AU6821696A (en) | 1997-03-12 |
| CA2228769A1 (en) | 1997-02-27 |
| EP0851937A1 (de) | 1998-07-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2228769C (en) | Method of purifying, stabilising or isolating nucleic acids contained in biological matter | |
| US6992182B1 (en) | Method for isolating DNA from biological materials | |
| EP3347491B1 (en) | Purification of nucleic acid from environmental or biological samples | |
| JP4776133B2 (ja) | 阻害物質に富む糞便試料及び他の生物学的材料からの核酸の単離 | |
| JPH10501246A (ja) | 極度に少量でかつ非常に強く汚染された非常に種々の出発物質から核酸を単離および精製する一般的方法 | |
| JP2022191311A (ja) | Ｍｉｔｒａチップ抽出を使用してｄｎａの変異を検出するための方法 | |
| EP2854981B1 (en) | Methods of nucleic acid fractionation and detection | |
| KR20070042912A (ko) | 정제된 ｒｎａ를 분리하기 위한 시약 및 방법 | |
| CN116287144B (zh) | 核酸检测系统、装置和方法 | |
| CN100429307C (zh) | 核酸检测方法及其系统 | |
| WO2014143714A2 (en) | Methods for one step nucleic acid amplification of non-eluted samples | |
| CN102834519A (zh) | 从复杂基质中提取核酸 | |
| CA2342393A1 (en) | A rapid pcr method for synthesizing dna | |
| US20120171728A1 (en) | Process for amplifying dna using tetratethylene glycol, kit of parts therefor and use thereof | |
| JP2003529314A (ja) | Ｄｎａを単離し、増幅し、そして特性化するための方法 | |
| EP2009441B1 (en) | Method for determining the amount of DNA binding protein | |
| US20020146739A1 (en) | Method for the affinity isolation of newly synthesized RNA | |
| EP4372083A1 (en) | Method for extracting nucleic acid from polyphenol-containing sample | |
| EP0533952A1 (en) | Process for separating or extracting nucleic acid from specimen containing the same | |
| KR20250044142A (ko) | 핵산 정제 또는 추출용 조성물 및 이의 용도 | |
| US20210189458A1 (en) | Methods and compositions for the purification of unbiased rna | |
| WO2012014694A1 (ja) | 糞便由来核酸の合成方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20160815 |