JP4264133B2 - 生物学的物質からの核酸を精製、安定化又は単離する方法 - Google Patents

生物学的物質からの核酸を精製、安定化又は単離する方法 Download PDF

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Publication number
JP4264133B2
JP4264133B2 JP50894597A JP50894597A JP4264133B2 JP 4264133 B2 JP4264133 B2 JP 4264133B2 JP 50894597 A JP50894597 A JP 50894597A JP 50894597 A JP50894597 A JP 50894597A JP 4264133 B2 JP4264133 B2 JP 4264133B2
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Prior art keywords
nucleic acid
sample
nucleic acids
stabilizing
purifying
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JP50894597A
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Japanese (ja)
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JPH11511020A (ja
JPH11511020A5 (enExample
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ミュラー オリヴァー
ドイター ライナー
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Max Planck Gesellschaft zur Foerderung der Wissenschaften
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Max Planck Gesellschaft zur Foerderung der Wissenschaften
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
  • Detergent Compositions (AREA)
  • Processing Of Solid Wastes (AREA)
  • Saccharide Compounds (AREA)
JP50894597A 1995-08-16 1996-08-14 生物学的物質からの核酸を精製、安定化又は単離する方法 Expired - Lifetime JP4264133B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19530132A DE19530132C2 (de) 1995-08-16 1995-08-16 Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien
DE19530132.3 1995-08-16
PCT/EP1996/003595 WO1997007239A1 (de) 1995-08-16 1996-08-14 Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien

Publications (3)

Publication Number Publication Date
JPH11511020A JPH11511020A (ja) 1999-09-28
JPH11511020A5 JPH11511020A5 (enExample) 2004-07-15
JP4264133B2 true JP4264133B2 (ja) 2009-05-13

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JP50894597A Expired - Lifetime JP4264133B2 (ja) 1995-08-16 1996-08-14 生物学的物質からの核酸を精製、安定化又は単離する方法

Country Status (9)

Country Link
US (1) US6084091A (enExample)
EP (1) EP0851937B1 (enExample)
JP (1) JP4264133B2 (enExample)
AT (1) ATE215611T1 (enExample)
AU (1) AU712331B2 (enExample)
CA (1) CA2228769C (enExample)
DE (2) DE19530132C2 (enExample)
DK (1) DK0851937T3 (enExample)
WO (1) WO1997007239A1 (enExample)

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US7995950B2 (en) 2005-07-15 2011-08-09 Ricoh Co., Ltd. Image forming apparatus, image forming method and process cartridge involving the use of a cleaning blade that removes toner remaining on a surface of an image bearing member

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US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
EP0939118A1 (en) * 1998-02-20 1999-09-01 Universiteit Maastricht Method for isolating DNA and RNA from faeces
ATE458822T1 (de) 1998-04-08 2010-03-15 Metabolix Inc Verfahren für die trennung und reinigung von biopolymeren
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DE19900638C2 (de) * 1999-01-11 2002-12-19 Max Planck Gesellschaft Methode zur Isolierung von DNA aus biologischen Materialien
JP2002537777A (ja) 1999-02-25 2002-11-12 エグザクト サイエンシーズ コーポレイション Ｄｎａの完全性を保存するための方法
EP1169479B1 (en) 1999-04-09 2006-06-28 EXACT Sciences Corporation Methods for detecting nucleic acids indicative of cancer
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6383783B1 (en) 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant
US6919174B1 (en) * 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
CA2394921A1 (en) 1999-12-07 2001-06-14 Anthony P. Shuber Supracolonic aerodigestive neoplasm detection
ATE376067T1 (de) * 2000-02-04 2007-11-15 Qiagen Gmbh Nukleinsäure-isolierung aus stuhlproben und anderen biologischen materialien, die reich an inhibitoren sind
AU2001238616A1 (en) * 2000-02-23 2001-09-03 Wen Shao Rapid nucleic acid separation, isolation and purification methods
GB0015777D0 (en) * 2000-06-28 2000-08-16 Tayside University Hospitals N DNA Extraction
DK1442045T3 (da) * 2001-11-06 2009-02-09 Cortex Biochem Inc Isolering og oprensning af nukleinsyrer
GB0130821D0 (en) * 2001-12-24 2002-02-06 Tayside University Hospitals N DNA screening
WO2003071252A2 (en) 2002-02-15 2003-08-28 Exact Sciences Corporation Methods for analysis of molecular events
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
US20040259101A1 (en) * 2003-06-20 2004-12-23 Shuber Anthony P. Methods for disease screening
EP1664295A4 (en) * 2003-09-09 2007-12-05 Fujifilm Corp PROCESS FOR INSULATING AND PURIFYING NUCLEIC ACID
CN101018867A (zh) * 2003-10-02 2007-08-15 密西西比州立大学 从废水处理厂的污泥中生产生物柴油和其它有价值的化学物质
US20050130177A1 (en) * 2003-12-12 2005-06-16 3M Innovative Properties Company Variable valve apparatus and methods
US7939249B2 (en) 2003-12-24 2011-05-10 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
US7727710B2 (en) 2003-12-24 2010-06-01 3M Innovative Properties Company Materials, methods, and kits for reducing nonspecific binding of molecules to a surface
US20050142571A1 (en) * 2003-12-24 2005-06-30 3M Innovative Properties Company Methods for nucleic acid isolation and kits using solid phase material
WO2005068662A1 (en) * 2003-12-30 2005-07-28 Sigma-Aldrich Co. Rapid preparation of nucleic acids by enzymatic digestion
US8426126B2 (en) 2004-03-18 2013-04-23 Applied Biosystems, Llc Modified surfaces as solid supports for nucleic acid purification
US20080241827A1 (en) * 2004-05-10 2008-10-02 Exact Sciences Corporation Methods For Detecting A Mutant Nucleic Acid
WO2006026654A2 (en) 2004-08-27 2006-03-09 Exact Sciences Corporation Method for detecting a recombinant event
US7195878B2 (en) * 2004-09-30 2007-03-27 Cryo-Genomics Device and method for fecal testing
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
WO2007044071A2 (en) * 2005-04-21 2007-04-19 Exact Sciences Corporation Analysis of heterogeneous nucleic acid samples
JP2009011247A (ja) * 2007-07-05 2009-01-22 Sumitomo Bakelite Co Ltd 遺伝子の検出方法
US20090111193A1 (en) * 2007-10-31 2009-04-30 Cooney Christopher G Sample preparation device
US9428746B2 (en) 2007-10-31 2016-08-30 Akonni Biosystems, Inc. Method and kit for purifying nucleic acids
US7759112B2 (en) * 2007-10-31 2010-07-20 Akonni Biosystems, Inc. Apparatus, system, and method for purifying nucleic acids
US10125388B2 (en) 2007-10-31 2018-11-13 Akonni Biosystems, Inc. Integrated sample processing system
EP2218791B1 (en) * 2007-11-05 2015-01-07 Eiken Kagaku Kabushiki Kaisha Method and kit for preparation of sample for use in nucleic acid amplification
JP5599013B2 (ja) * 2008-04-03 2014-10-01 キヤノン株式会社 血液検体からの微生物核酸の抽出方法
JP5710969B2 (ja) * 2008-07-23 2015-04-30 オリンパス株式会社 糞便試料からの核酸回収方法
US20100121046A1 (en) * 2008-09-05 2010-05-13 Mayo Foundation For Medical Education And Research Collecting and processing complex macromolecular mixtures
JPWO2010134245A1 (ja) * 2009-05-20 2012-11-08 オリンパス株式会社 哺乳細胞由来核酸の回収方法、核酸解析方法、及び採便用キット
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US8222397B2 (en) * 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
PT2521780T (pt) 2010-01-07 2017-12-21 Bigtec Private Ltd Um método para isolamento de ácidos nucleicos e um kit do mesmo
JP5743701B2 (ja) * 2011-05-10 2015-07-01 英二 小西 Ｒｎａ検出用試薬およびｒｎａ検出方法
JP5743704B2 (ja) * 2011-05-12 2015-07-01 住友大阪セメント株式会社 Ｒｎａ検出用試薬およびｒｎａ検出方法
EP2721140B1 (en) 2011-06-19 2016-11-23 Abogen, Inc. Devices, solutions and methods for sample collection
EP2753693B1 (en) * 2011-09-06 2016-04-13 North-West University A method of preparing biological material
WO2013067509A1 (en) * 2011-11-04 2013-05-10 Bio-Rad Laboratories, Inc. Simultaneous purification of cell components
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
US9658195B2 (en) 2012-02-15 2017-05-23 Bio-Rad Laboratories, Inc. Electronic control of pH and ionic strength
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
SG11201607364RA (en) 2014-03-07 2016-10-28 Dna Genotek Inc Composition and method for stabilizing nucleic acids in biological samples
CN108368539A (zh) 2015-09-10 2018-08-03 生命技术公司 环境或生物样本中的核酸纯化
CN113832141B (zh) * 2021-09-30 2024-03-08 苏州唯善生物科技有限公司 粪便人源基因组dna特异性纯化方法及试剂盒

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7995950B2 (en) 2005-07-15 2011-08-09 Ricoh Co., Ltd. Image forming apparatus, image forming method and process cartridge involving the use of a cleaning blade that removes toner remaining on a surface of an image bearing member
US7851114B2 (en) 2005-11-21 2010-12-14 Ricoh Company Limited Electrostatic latent image bearing member, and image forming apparatus, process cartridge, and image forming method using the same

Also Published As

Publication number Publication date
JPH11511020A (ja) 1999-09-28
EP0851937A1 (de) 1998-07-08
CA2228769A1 (en) 1997-02-27
EP0851937B1 (de) 2002-04-03
DK0851937T3 (da) 2002-06-24
DE59609027D1 (de) 2002-05-08
WO1997007239A1 (de) 1997-02-27
US6084091A (en) 2000-07-04
ATE215611T1 (de) 2002-04-15
DE19530132C2 (de) 1998-07-16
AU712331B2 (en) 1999-11-04
CA2228769C (en) 2011-04-12
DE19530132A1 (de) 1997-02-20
AU6821696A (en) 1997-03-12

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