DE19530132C2 - Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien - Google Patents
Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen MaterialienInfo
- Publication number
- DE19530132C2 DE19530132C2 DE19530132A DE19530132A DE19530132C2 DE 19530132 C2 DE19530132 C2 DE 19530132C2 DE 19530132 A DE19530132 A DE 19530132A DE 19530132 A DE19530132 A DE 19530132A DE 19530132 C2 DE19530132 C2 DE 19530132C2
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acids
- adsorption matrix
- reagent kit
- sample
- isolation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Detergent Compositions (AREA)
- Processing Of Solid Wastes (AREA)
- Saccharide Compounds (AREA)
Priority Applications (10)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19530132A DE19530132C2 (de) | 1995-08-16 | 1995-08-16 | Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien |
| EP96928466A EP0851937B1 (de) | 1995-08-16 | 1996-08-14 | Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien |
| AU68216/96A AU712331B2 (en) | 1995-08-16 | 1996-08-14 | Process for purifying, stabilising or isolating nucleic acids contained in biological materials |
| AT96928466T ATE215611T1 (de) | 1995-08-16 | 1996-08-14 | Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien |
| DK96928466T DK0851937T3 (da) | 1995-08-16 | 1996-08-14 | Fremgangsmåde til oprensning, stabilisering eller isolering af nucleinsyrer fra biologiske materialer |
| DE59609027T DE59609027D1 (de) | 1995-08-16 | 1996-08-14 | Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien |
| US09/011,567 US6084091A (en) | 1995-08-16 | 1996-08-14 | Process for purifying, stabilising or isolating nucleic acids from biological materials |
| JP50894597A JP4264133B2 (ja) | 1995-08-16 | 1996-08-14 | 生物学的物質からの核酸を精製、安定化又は単離する方法 |
| CA2228769A CA2228769C (en) | 1995-08-16 | 1996-08-14 | Method of purifying, stabilising or isolating nucleic acids contained in biological matter |
| PCT/EP1996/003595 WO1997007239A1 (de) | 1995-08-16 | 1996-08-14 | Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19530132A DE19530132C2 (de) | 1995-08-16 | 1995-08-16 | Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE19530132A1 DE19530132A1 (de) | 1997-02-20 |
| DE19530132C2 true DE19530132C2 (de) | 1998-07-16 |
Family
ID=7769624
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19530132A Expired - Lifetime DE19530132C2 (de) | 1995-08-16 | 1995-08-16 | Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien |
| DE59609027T Expired - Lifetime DE59609027D1 (de) | 1995-08-16 | 1996-08-14 | Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE59609027T Expired - Lifetime DE59609027D1 (de) | 1995-08-16 | 1996-08-14 | Verfahren zur reinigung, stabilisierung oder isolierung von nukleinsäuren aus biologischen materialien |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US6084091A (enExample) |
| EP (1) | EP0851937B1 (enExample) |
| JP (1) | JP4264133B2 (enExample) |
| AT (1) | ATE215611T1 (enExample) |
| AU (1) | AU712331B2 (enExample) |
| CA (1) | CA2228769C (enExample) |
| DE (2) | DE19530132C2 (enExample) |
| DK (1) | DK0851937T3 (enExample) |
| WO (1) | WO1997007239A1 (enExample) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6849403B1 (en) | 1999-09-08 | 2005-02-01 | Exact Sciences Corporation | Apparatus and method for drug screening |
| US6919174B1 (en) | 1999-12-07 | 2005-07-19 | Exact Sciences Corporation | Methods for disease detection |
| US6964846B1 (en) | 1999-04-09 | 2005-11-15 | Exact Sciences Corporation | Methods for detecting nucleic acids indicative of cancer |
| US7368233B2 (en) | 1999-12-07 | 2008-05-06 | Exact Sciences Corporation | Methods of screening for lung neoplasm based on stool samples containing a nucleic acid marker indicative of a neoplasm |
| US7811757B2 (en) | 1999-09-08 | 2010-10-12 | Genzyme Corporation | Methods for disease detection |
Families Citing this family (59)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE59712894D1 (de) * | 1996-08-26 | 2007-12-27 | Invitek Biotechnik & Biodesign | Verfahren zum nachweis klinisch relevanter veränderungen der dns-sequenz des ki-ras-onkogens, seine verwendung und testkit zur früherkennung von tumoren |
| AU7816898A (en) * | 1997-06-16 | 1999-01-04 | Exact Laboratories, Inc. | Methods for stool sample preparation |
| US6406857B1 (en) | 1997-06-16 | 2002-06-18 | Exact Sciences Corporation | Methods for stool sample preparation |
| US6268136B1 (en) | 1997-06-16 | 2001-07-31 | Exact Science Corporation | Methods for stool sample preparation |
| US6241980B1 (en) | 1997-11-04 | 2001-06-05 | Becton, Dickinson And Company | Sample processing method using ion exchange resin |
| US7569342B2 (en) | 1997-12-10 | 2009-08-04 | Sierra Molecular Corp. | Removal of molecular assay interferences |
| EP0939118A1 (en) * | 1998-02-20 | 1999-09-01 | Universiteit Maastricht | Method for isolating DNA and RNA from faeces |
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| US6964846B1 (en) | 1999-04-09 | 2005-11-15 | Exact Sciences Corporation | Methods for detecting nucleic acids indicative of cancer |
| US6849403B1 (en) | 1999-09-08 | 2005-02-01 | Exact Sciences Corporation | Apparatus and method for drug screening |
| US7811757B2 (en) | 1999-09-08 | 2010-10-12 | Genzyme Corporation | Methods for disease detection |
| US6919174B1 (en) | 1999-12-07 | 2005-07-19 | Exact Sciences Corporation | Methods for disease detection |
| US7368233B2 (en) | 1999-12-07 | 2008-05-06 | Exact Sciences Corporation | Methods of screening for lung neoplasm based on stool samples containing a nucleic acid marker indicative of a neoplasm |
| US7981612B2 (en) | 1999-12-07 | 2011-07-19 | Mayo Foundation For Medical Education And Research | Methods of screening for supracolonic neoplasms based on stool samples containing a nucleic acid marker indicative of a neoplasm |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH11511020A (ja) | 1999-09-28 |
| EP0851937A1 (de) | 1998-07-08 |
| CA2228769A1 (en) | 1997-02-27 |
| EP0851937B1 (de) | 2002-04-03 |
| DK0851937T3 (da) | 2002-06-24 |
| DE59609027D1 (de) | 2002-05-08 |
| WO1997007239A1 (de) | 1997-02-27 |
| US6084091A (en) | 2000-07-04 |
| ATE215611T1 (de) | 2002-04-15 |
| AU712331B2 (en) | 1999-11-04 |
| CA2228769C (en) | 2011-04-12 |
| DE19530132A1 (de) | 1997-02-20 |
| AU6821696A (en) | 1997-03-12 |
| JP4264133B2 (ja) | 2009-05-13 |
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