CA2195206A1 - Cereales transgeniques - Google Patents

Cereales transgeniques

Info

Publication number
CA2195206A1
CA2195206A1 CA 2195206 CA2195206A CA2195206A1 CA 2195206 A1 CA2195206 A1 CA 2195206A1 CA 2195206 CA2195206 CA 2195206 CA 2195206 A CA2195206 A CA 2195206A CA 2195206 A1 CA2195206 A1 CA 2195206A1
Authority
CA
Canada
Prior art keywords
meristem
cells
transgenic
shoot
transformed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2195206
Other languages
English (en)
Inventor
Benjamin A. Bowen
Keith Lowe
Margot C. Ross
Gary A. Sandahl
Dwight T. Tomes
David D. Songtad
William J. Gordon-Kamm
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pioneer Hi Bred International Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/483,091 external-priority patent/US5736369A/en
Application filed by Individual filed Critical Individual
Publication of CA2195206A1 publication Critical patent/CA2195206A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Cereal-Derived Products (AREA)
  • Noodles (AREA)

Abstract

Pour obtenir une céréale transgénique qui est transformée d'une manière stable, on soumet un méristème de céréale qui a été dégagé, à un bombardement biolistique, en vue d'une transformation des cellules non différenciées du méristème. On recueille des embryons immatures au stade pro-embryonnaire précoce, moyen et tardif, au stade transitionnel ou au stade coléoptilaire précoce pour effectuer le bombardement biolistique. On soumet le tissu du méristème ou des cellules devant contribuer à la formation du méristème à une manipulation pour élargir les secteurs transgéniques, par sélection et/ou en provoquant une prolifération à partir du tissu de pousses ou de méristèmes multiples en eux-mêmes. La population de pousses ainsi obtenue est soumise à une sélection par un test d'enrichissement non létal, pour identifier les secteurs chimères qui vont contribuer à la transmission de la lignée germinale ou des chimères périclinales non sectorisées L2 qui vont, par définition, se transmettre à la progéniture. Une augmentation du temps de culture dans des conditions de sélection augmente les chances de conversions sectorielle en périclinale et également favorise les conversions L1 en L2, qui, par un décalage de position, contribuent en fin de compte à la lignée germinale. Les secteurs transgéniques sont également stabilisés pendant la phase de tallage.
CA 2195206 1994-07-29 1995-07-26 Cereales transgeniques Abandoned CA2195206A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US28227094A 1994-07-29 1994-07-29
US08/282,270 1994-07-29
US08/483,091 1995-06-07
US08/483,091 US5736369A (en) 1994-07-29 1995-06-07 Method for producing transgenic cereal plants

Publications (1)

Publication Number Publication Date
CA2195206A1 true CA2195206A1 (fr) 1996-02-15

Family

ID=26961344

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2195206 Abandoned CA2195206A1 (fr) 1994-07-29 1995-07-26 Cereales transgeniques

Country Status (9)

Country Link
EP (1) EP0772687A2 (fr)
JP (1) JPH10503374A (fr)
AU (1) AU697373B2 (fr)
BR (1) BR9508341A (fr)
CA (1) CA2195206A1 (fr)
HU (1) HUT76841A (fr)
MX (1) MX199137B (fr)
NZ (1) NZ293737A (fr)
WO (1) WO1996004392A2 (fr)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7102056B1 (en) 1997-04-29 2006-09-05 The Regents Of The University Of California Compositions and methods for plant transformation and regeneration
US6235529B1 (en) 1997-04-29 2001-05-22 The Regents Of The University Of California Compositions and methods for plant transformation and regeneration
EP1017268A1 (fr) 1997-09-24 2000-07-12 The Regents Of The University Of California Procedes et compositions pour la transformation de cereales au moyen de tissu merismatique de pousses de culture
ES2276475T5 (es) 1997-09-30 2014-07-11 The Regents Of The University Of California Producción de proteínas en semillas de plantas
US6153811A (en) * 1997-12-22 2000-11-28 Dekalb Genetics Corporation Method for reduction of transgene copy number
US6096546A (en) * 1998-01-30 2000-08-01 Board Of Trustees, Rutgers, The State University Of New Jersey Methods for recovering polypeptides from plants and portions thereof
US6100092A (en) 1998-06-15 2000-08-08 Board Of Trustees, Rutgers The State University Of New Jersey Materials and methods for amplifying polynucleotides in plants
EP1261729A2 (fr) * 2000-02-25 2002-12-04 Villoo Morawala Patell Methode de production d'un millet a chandelles genetiquement modifie par agrobacterie et transformation biolistique
AU2001255050B2 (en) * 2000-02-25 2005-07-14 Avestha Gengraine Technologies Pvt. Ltd. A process for generating genetically modified pearl millet through agrobacteriumand biolistic transformation
US6544789B1 (en) 2000-03-13 2003-04-08 Board Of Trustees, Rutgers, The State University Of New Jersey Phosphorus-controllable recombinant expression of polypeptides in plants
IT1317038B1 (it) * 2000-06-05 2003-05-26 Vitroplant Vivai Di Zuccherell Metodo per la rigenerazione di piante e suoi usi per lamoltiplicazione e/o la trasformazione di piante.
WO2003007698A2 (fr) * 2001-07-19 2003-01-30 Monsanto Technology Llc Nouveau procede de production de plantes transgeniques
KR20030010234A (ko) * 2001-07-26 2003-02-05 권오경 차동 신호 검출회로
DE10201637A1 (de) * 2002-01-17 2003-08-07 Univ Albert Ludwigs Freiburg Verfahren zur Herstellung einer Mais-Kalluskultur
US7429691B2 (en) 2002-09-03 2008-09-30 The Regents Of The University Of California Methods and compositions for transformation and regeneration of maize
EP1578193A4 (fr) 2002-12-23 2011-06-15 Vical Inc Procede de lyophilisation de complexes d'acide nucleique/copolymere sequence/tensioactif cationique
WO2004060363A1 (fr) 2002-12-23 2004-07-22 Vical Incorporated Procede permettant de produire des medicaments steriles a base de polynucleotides
WO2008120820A1 (fr) 2007-03-29 2008-10-09 International Flower Developments Proprietary Limited Procédé pour produire une plante chimérique à surface transgénique
JP2016531559A (ja) 2013-10-04 2016-10-13 ダウ アグロサイエンシィズ エルエルシー ダイズ形質転換法
EP3115456A4 (fr) * 2014-03-06 2017-10-25 Riken Procédé de transformation de plantes
EP3456181A4 (fr) * 2016-05-13 2019-12-25 Kaneka Corporation Procédé de création de plante transformée
WO2017195906A1 (fr) 2016-05-13 2017-11-16 株式会社カネカ Procédé d'édition du génome d'une plante
US11499158B2 (en) 2016-05-13 2022-11-15 Kaneka Corporation Method for modifying plant

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5177010A (en) * 1986-06-30 1993-01-05 University Of Toledo Process for transforming corn and the products thereof
DK240088A (da) * 1987-05-05 1988-11-06 Sandoz Ag Fremgangsmaade til transformation af et plantecellematerale
FI890917A (fi) * 1988-03-02 1989-09-03 Eidgenoess Tech Hochschule Foerfarande foer framstaellning av transgena vaexter.
CA2017544A1 (fr) * 1989-05-29 1990-11-29 Gunther Neuhaus Procede de production de plants transgeniques
DE4013099A1 (de) * 1990-04-25 1991-10-31 Hoechst Ag Verfahren zur transformation somatischer embryonen von nutzpflanzen
DE69232132T3 (de) * 1991-05-15 2008-08-14 Monsanto Technology Llc. Verfahren zur schöpfung einer transformierten reispflanze
US5304719A (en) * 1992-02-03 1994-04-19 Pioneer Hi-Bred International, Inc. Inbred corn line PHT47
FI933682A0 (fi) * 1993-08-20 1993-08-20 Valtion Teknillinen Metod foer att producera transgena saedesvaexter

Also Published As

Publication number Publication date
AU697373B2 (en) 1998-10-01
AU3624795A (en) 1996-03-04
MX199137B (es) 2000-10-18
EP0772687A2 (fr) 1997-05-14
BR9508341A (pt) 1997-08-12
JPH10503374A (ja) 1998-03-31
WO1996004392A3 (fr) 1996-03-28
WO1996004392A2 (fr) 1996-02-15
HUT76841A (en) 1997-11-28
MX9700688A (es) 1998-01-31
NZ293737A (en) 1998-07-28

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