CA2195206A1 - Cereales transgeniques - Google Patents
Cereales transgeniquesInfo
- Publication number
- CA2195206A1 CA2195206A1 CA 2195206 CA2195206A CA2195206A1 CA 2195206 A1 CA2195206 A1 CA 2195206A1 CA 2195206 CA2195206 CA 2195206 CA 2195206 A CA2195206 A CA 2195206A CA 2195206 A1 CA2195206 A1 CA 2195206A1
- Authority
- CA
- Canada
- Prior art keywords
- meristem
- cells
- transgenic
- shoot
- transformed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
- C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Cereal-Derived Products (AREA)
- Noodles (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Pour obtenir une céréale transgénique qui est transformée d'une manière stable, on soumet un méristème de céréale qui a été dégagé, à un bombardement biolistique, en vue d'une transformation des cellules non différenciées du méristème. On recueille des embryons immatures au stade pro-embryonnaire précoce, moyen et tardif, au stade transitionnel ou au stade coléoptilaire précoce pour effectuer le bombardement biolistique. On soumet le tissu du méristème ou des cellules devant contribuer à la formation du méristème à une manipulation pour élargir les secteurs transgéniques, par sélection et/ou en provoquant une prolifération à partir du tissu de pousses ou de méristèmes multiples en eux-mêmes. La population de pousses ainsi obtenue est soumise à une sélection par un test d'enrichissement non létal, pour identifier les secteurs chimères qui vont contribuer à la transmission de la lignée germinale ou des chimères périclinales non sectorisées L2 qui vont, par définition, se transmettre à la progéniture. Une augmentation du temps de culture dans des conditions de sélection augmente les chances de conversions sectorielle en périclinale et également favorise les conversions L1 en L2, qui, par un décalage de position, contribuent en fin de compte à la lignée germinale. Les secteurs transgéniques sont également stabilisés pendant la phase de tallage.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US28227094A | 1994-07-29 | 1994-07-29 | |
US08/282,270 | 1994-07-29 | ||
US08/483,091 US5736369A (en) | 1994-07-29 | 1995-06-07 | Method for producing transgenic cereal plants |
US08/483,091 | 1995-06-07 |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2195206A1 true CA2195206A1 (fr) | 1996-02-15 |
Family
ID=26961344
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA 2195206 Abandoned CA2195206A1 (fr) | 1994-07-29 | 1995-07-26 | Cereales transgeniques |
Country Status (9)
Country | Link |
---|---|
EP (1) | EP0772687A2 (fr) |
JP (1) | JPH10503374A (fr) |
AU (1) | AU697373B2 (fr) |
BR (1) | BR9508341A (fr) |
CA (1) | CA2195206A1 (fr) |
HU (1) | HUT76841A (fr) |
MX (1) | MX199137B (fr) |
NZ (1) | NZ293737A (fr) |
WO (1) | WO1996004392A2 (fr) |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7102056B1 (en) | 1997-04-29 | 2006-09-05 | The Regents Of The University Of California | Compositions and methods for plant transformation and regeneration |
US6235529B1 (en) | 1997-04-29 | 2001-05-22 | The Regents Of The University Of California | Compositions and methods for plant transformation and regeneration |
EP1017268A1 (fr) | 1997-09-24 | 2000-07-12 | The Regents Of The University Of California | Procedes et compositions pour la transformation de cereales au moyen de tissu merismatique de pousses de culture |
ES2276475T5 (es) | 1997-09-30 | 2014-07-11 | The Regents Of The University Of California | Producción de proteínas en semillas de plantas |
US6153811A (en) * | 1997-12-22 | 2000-11-28 | Dekalb Genetics Corporation | Method for reduction of transgene copy number |
US6096546A (en) * | 1998-01-30 | 2000-08-01 | Board Of Trustees, Rutgers, The State University Of New Jersey | Methods for recovering polypeptides from plants and portions thereof |
US6100092A (en) * | 1998-06-15 | 2000-08-08 | Board Of Trustees, Rutgers The State University Of New Jersey | Materials and methods for amplifying polynucleotides in plants |
AU2001255050B2 (en) * | 2000-02-25 | 2005-07-14 | Avestha Gengraine Technologies Pvt. Ltd. | A process for generating genetically modified pearl millet through agrobacteriumand biolistic transformation |
CN1426476A (zh) * | 2000-02-25 | 2003-06-25 | 阿维斯塔金格兰技术有限公司 | 通过农杆菌属和biolistic转化制备遗传修饰过的珍珠稗的方法 |
US6544789B1 (en) | 2000-03-13 | 2003-04-08 | Board Of Trustees, Rutgers, The State University Of New Jersey | Phosphorus-controllable recombinant expression of polypeptides in plants |
IT1317038B1 (it) * | 2000-06-05 | 2003-05-26 | Vitroplant Vivai Di Zuccherell | Metodo per la rigenerazione di piante e suoi usi per lamoltiplicazione e/o la trasformazione di piante. |
US8212109B2 (en) | 2001-07-19 | 2012-07-03 | Monsanto Technology Llc | Method for the production of transgenic plants |
KR20030010234A (ko) * | 2001-07-26 | 2003-02-05 | 권오경 | 차동 신호 검출회로 |
DE10201637A1 (de) * | 2002-01-17 | 2003-08-07 | Univ Albert Ludwigs Freiburg | Verfahren zur Herstellung einer Mais-Kalluskultur |
US7429691B2 (en) | 2002-09-03 | 2008-09-30 | The Regents Of The University Of California | Methods and compositions for transformation and regeneration of maize |
CA2508279A1 (fr) | 2002-12-23 | 2004-07-22 | Vical Incorporated | Procede de lyophilisation de complexes d'acide nucleique/copolymere sequence/tensioactif cationique |
US7381422B2 (en) | 2002-12-23 | 2008-06-03 | Vical Incorporated | Method for producing sterile polynucleotide based medicaments |
CA2682095C (fr) | 2007-03-29 | 2014-06-03 | International Flower Developments Proprietary Limited | Procede pour produire une plante chimerique a surface transgenique |
CN105792642A (zh) | 2013-10-04 | 2016-07-20 | 美国陶氏益农公司 | 大豆转化方法 |
EP3115456A4 (fr) | 2014-03-06 | 2017-10-25 | Riken | Procédé de transformation de plantes |
US11499158B2 (en) | 2016-05-13 | 2022-11-15 | Kaneka Corporation | Method for modifying plant |
JP2017205104A (ja) * | 2016-05-13 | 2017-11-24 | 株式会社カネカ | 植物のゲノム編集方法 |
JP6967217B2 (ja) * | 2016-05-13 | 2021-11-17 | 株式会社カネカ | 形質転換植物の作製方法 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5177010A (en) * | 1986-06-30 | 1993-01-05 | University Of Toledo | Process for transforming corn and the products thereof |
AU615905B2 (en) * | 1987-05-05 | 1991-10-17 | Novartis Ag | Plant tissue transformation |
FI890917A (fi) * | 1988-03-02 | 1989-09-03 | Eidgenoess Tech Hochschule | Foerfarande foer framstaellning av transgena vaexter. |
FI902635A0 (fi) * | 1989-05-29 | 1990-05-25 | Eidgenoess Tech Hochschule | Foerfarande foer framstaellning av transgena vaexter. |
DE4013099A1 (de) * | 1990-04-25 | 1991-10-31 | Hoechst Ag | Verfahren zur transformation somatischer embryonen von nutzpflanzen |
WO1992020809A1 (fr) * | 1991-05-15 | 1992-11-26 | Agracetus, Inc. | Procede de creation d'un riz transforme |
US5304719A (en) * | 1992-02-03 | 1994-04-19 | Pioneer Hi-Bred International, Inc. | Inbred corn line PHT47 |
FI933682A0 (fi) * | 1993-08-20 | 1993-08-20 | Valtion Teknillinen | Metod foer att producera transgena saedesvaexter |
-
1995
- 1995-07-26 BR BR9508341A patent/BR9508341A/pt not_active Application Discontinuation
- 1995-07-26 HU HU9700247V patent/HUT76841A/hu unknown
- 1995-07-26 EP EP95933706A patent/EP0772687A2/fr not_active Withdrawn
- 1995-07-26 MX MX9700688A patent/MX199137B/es unknown
- 1995-07-26 AU AU36247/95A patent/AU697373B2/en not_active Ceased
- 1995-07-26 NZ NZ293737A patent/NZ293737A/en unknown
- 1995-07-26 JP JP8506538A patent/JPH10503374A/ja not_active Ceased
- 1995-07-26 WO PCT/US1995/008977 patent/WO1996004392A2/fr not_active Application Discontinuation
- 1995-07-26 CA CA 2195206 patent/CA2195206A1/fr not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
HUT76841A (en) | 1997-11-28 |
AU3624795A (en) | 1996-03-04 |
EP0772687A2 (fr) | 1997-05-14 |
BR9508341A (pt) | 1997-08-12 |
JPH10503374A (ja) | 1998-03-31 |
AU697373B2 (en) | 1998-10-01 |
WO1996004392A2 (fr) | 1996-02-15 |
MX9700688A (es) | 1998-01-31 |
WO1996004392A3 (fr) | 1996-03-28 |
NZ293737A (en) | 1998-07-28 |
MX199137B (es) | 2000-10-18 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
FZDE | Dead |