WO1996004392A3 - Cereales transgeniques - Google Patents

Cereales transgeniques Download PDF

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Publication number
WO1996004392A3
WO1996004392A3 PCT/US1995/008977 US9508977W WO9604392A3 WO 1996004392 A3 WO1996004392 A3 WO 1996004392A3 US 9508977 W US9508977 W US 9508977W WO 9604392 A3 WO9604392 A3 WO 9604392A3
Authority
WO
WIPO (PCT)
Prior art keywords
meristem
contribute
proembryo
transgenic
sectors
Prior art date
Application number
PCT/US1995/008977
Other languages
English (en)
Other versions
WO1996004392A2 (fr
Inventor
Benjamin A Bowen
Keith Lowe
Margot C Ross
Gary A Sandahl
Dwight T Tomes
David D Songstad
William J Gordon-Kamm
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/483,091 external-priority patent/US5736369A/en
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Priority to AU36247/95A priority Critical patent/AU697373B2/en
Priority to NZ293737A priority patent/NZ293737A/en
Priority to MX9700688A priority patent/MX199137B/es
Priority to EP95933706A priority patent/EP0772687A2/fr
Priority to BR9508341A priority patent/BR9508341A/pt
Priority to JP8506538A priority patent/JPH10503374A/ja
Publication of WO1996004392A2 publication Critical patent/WO1996004392A2/fr
Publication of WO1996004392A3 publication Critical patent/WO1996004392A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers

Abstract

Pour obtenir une céréale transgénique qui est transformée d'une manière stable, on soumet un méristème de céréale qui a été dégagé, à un bombardement biolistique, en vue d'une transformation des cellules non différenciées du méristème. On recueille des embryons immatures au stade pro-embryonnaire précoce, moyen et tardif, au stade transitionnel ou au stade coléoptilaire précoce pour effectuer le bombardement biolistique. On soumet le tissu du méristème ou des cellules devant contribuer à la formation du méristème à une manipulation pour élargir les secteurs transgéniques, par sélection et/ou en provoquant une prolifération à partir du tissu de pousses ou de méristèmes multiples en eux-mêmes. La population de pousses ainsi obtenue est soumise à une sélection par un test d'enrichissement non létal, pour identifier les secteurs chimères qui vont contribuer à la transmission de la lignée germinale ou des chimères périclinales non sectorisées L2 qui vont, par définition, se transmettre à la progéniture. Une augmentation du temps de culture dans des conditions de sélection augmente les chances de conversions sectorielle en périclinale et également favorise les conversions L1 en L2, qui, par un décalage de position, contribuent en fin de compte à la lignée germinale. Les secteurs transgéniques sont également stabilisés pendant la phase de tallage.
PCT/US1995/008977 1994-07-29 1995-07-26 Cereales transgeniques WO1996004392A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AU36247/95A AU697373B2 (en) 1994-07-29 1995-07-26 Transgenic cereal plants
NZ293737A NZ293737A (en) 1994-07-29 1995-07-26 A method of producing transgenic cereal plants
MX9700688A MX199137B (es) 1994-07-29 1995-07-26 Plantas transgenicas de cereal.
EP95933706A EP0772687A2 (fr) 1994-07-29 1995-07-26 Cereales transgeniques
BR9508341A BR9508341A (pt) 1994-07-29 1995-07-26 Processo para produzir plantas de cereal transgênicas
JP8506538A JPH10503374A (ja) 1994-07-29 1995-07-26 遺伝子導入禾穀類植物

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US28227094A 1994-07-29 1994-07-29
US08/282,270 1994-07-29
US08/483,091 US5736369A (en) 1994-07-29 1995-06-07 Method for producing transgenic cereal plants
US08/483,091 1995-06-07

Publications (2)

Publication Number Publication Date
WO1996004392A2 WO1996004392A2 (fr) 1996-02-15
WO1996004392A3 true WO1996004392A3 (fr) 1996-03-28

Family

ID=26961344

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1995/008977 WO1996004392A2 (fr) 1994-07-29 1995-07-26 Cereales transgeniques

Country Status (9)

Country Link
EP (1) EP0772687A2 (fr)
JP (1) JPH10503374A (fr)
AU (1) AU697373B2 (fr)
BR (1) BR9508341A (fr)
CA (1) CA2195206A1 (fr)
HU (1) HUT76841A (fr)
MX (1) MX199137B (fr)
NZ (1) NZ293737A (fr)
WO (1) WO1996004392A2 (fr)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7102056B1 (en) 1997-04-29 2006-09-05 The Regents Of The University Of California Compositions and methods for plant transformation and regeneration
US6235529B1 (en) 1997-04-29 2001-05-22 The Regents Of The University Of California Compositions and methods for plant transformation and regeneration
EP1017268A1 (fr) 1997-09-24 2000-07-12 The Regents Of The University Of California Procedes et compositions pour la transformation de cereales au moyen de tissu merismatique de pousses de culture
EP1019517B2 (fr) 1997-09-30 2014-05-21 The Regents of The University of California Fabrication de proteines dans des graines de plantes
US6153811A (en) * 1997-12-22 2000-11-28 Dekalb Genetics Corporation Method for reduction of transgene copy number
US6096546A (en) * 1998-01-30 2000-08-01 Board Of Trustees, Rutgers, The State University Of New Jersey Methods for recovering polypeptides from plants and portions thereof
US6100092A (en) * 1998-06-15 2000-08-08 Board Of Trustees, Rutgers The State University Of New Jersey Materials and methods for amplifying polynucleotides in plants
AU2001255050B2 (en) * 2000-02-25 2005-07-14 Avestha Gengraine Technologies Pvt. Ltd. A process for generating genetically modified pearl millet through agrobacteriumand biolistic transformation
AU5505001A (en) * 2000-02-25 2001-09-03 Villoo Morawala Patell A process for generating genetically modified pearl millet through agrobacteriumand biolistic transformation
US6544789B1 (en) 2000-03-13 2003-04-08 Board Of Trustees, Rutgers, The State University Of New Jersey Phosphorus-controllable recombinant expression of polypeptides in plants
IT1317038B1 (it) * 2000-06-05 2003-05-26 Vitroplant Vivai Di Zuccherell Metodo per la rigenerazione di piante e suoi usi per lamoltiplicazione e/o la trasformazione di piante.
WO2003007698A2 (fr) * 2001-07-19 2003-01-30 Monsanto Technology Llc Nouveau procede de production de plantes transgeniques
KR20030010234A (ko) * 2001-07-26 2003-02-05 권오경 차동 신호 검출회로
DE10201637A1 (de) * 2002-01-17 2003-08-07 Univ Albert Ludwigs Freiburg Verfahren zur Herstellung einer Mais-Kalluskultur
WO2004022707A2 (fr) 2002-09-03 2004-03-18 The Regents Of The University Of California Procedes et compositions utilises pour la transformation et la regeneration du mais
AU2003293196A1 (en) 2002-12-23 2004-07-29 Vical Incorporated Method for producing sterile polynucleotide based medicaments
WO2004060059A2 (fr) 2002-12-23 2004-07-22 Vical Incorporated Procede de lyophilisation de complexes d'acide nucleique/copolymere sequence/tensioactif cationique
CN101675161A (zh) 2007-03-29 2010-03-17 国际花卉开发有限公司 表层嵌合体（surface chimera）转基因植物的培育方法
CN105792642A (zh) * 2013-10-04 2016-07-20 美国陶氏益农公司 大豆转化方法
JPWO2015133652A1 (ja) * 2014-03-06 2017-04-06 国立研究開発法人理化学研究所 植物の形質転換法
JP2017205104A (ja) * 2016-05-13 2017-11-24 株式会社カネカ 植物のゲノム編集方法
EP3456181A4 (fr) 2016-05-13 2019-12-25 Kaneka Corporation Procédé de création de plante transformée
US11499158B2 (en) 2016-05-13 2022-11-15 Kaneka Corporation Method for modifying plant

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0290395A2 (fr) * 1987-05-05 1988-11-09 Sandoz Ag Transformation de tissus de plantes
EP0331083A2 (fr) * 1988-03-02 1989-09-06 Schweizerische Eidgenossenschaft Eidgenössische Technische Hochschule (Eth) Procédé pour la production de plantes transgéniques
EP0400553A1 (fr) * 1989-05-29 1990-12-05 Schweizerische Eidgenossenschaft Eidgenössische Technische Hochschule (Eth) Méthode pour la production de plantes transgéniques
DE4013099A1 (de) * 1990-04-25 1991-10-31 Hoechst Ag Verfahren zur transformation somatischer embryonen von nutzpflanzen
WO1992020809A1 (fr) * 1991-05-15 1992-11-26 Agracetus, Inc. Procede de creation d'un riz transforme
US5177010A (en) * 1986-06-30 1993-01-05 University Of Toledo Process for transforming corn and the products thereof
US5304719A (en) * 1992-02-03 1994-04-19 Pioneer Hi-Bred International, Inc. Inbred corn line PHT47
WO1995006127A1 (fr) * 1993-08-20 1995-03-02 Valtion Teknillinen Tutkimuskeskus Procede pour la production de cereales transgeniques

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5177010A (en) * 1986-06-30 1993-01-05 University Of Toledo Process for transforming corn and the products thereof
EP0290395A2 (fr) * 1987-05-05 1988-11-09 Sandoz Ag Transformation de tissus de plantes
EP0331083A2 (fr) * 1988-03-02 1989-09-06 Schweizerische Eidgenossenschaft Eidgenössische Technische Hochschule (Eth) Procédé pour la production de plantes transgéniques
EP0400553A1 (fr) * 1989-05-29 1990-12-05 Schweizerische Eidgenossenschaft Eidgenössische Technische Hochschule (Eth) Méthode pour la production de plantes transgéniques
DE4013099A1 (de) * 1990-04-25 1991-10-31 Hoechst Ag Verfahren zur transformation somatischer embryonen von nutzpflanzen
WO1992020809A1 (fr) * 1991-05-15 1992-11-26 Agracetus, Inc. Procede de creation d'un riz transforme
US5304719A (en) * 1992-02-03 1994-04-19 Pioneer Hi-Bred International, Inc. Inbred corn line PHT47
WO1995006127A1 (fr) * 1993-08-20 1995-03-02 Valtion Teknillinen Tutkimuskeskus Procede pour la production de cereales transgeniques

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BILANG, R., ET AL.: "Transient gene expression in vegetative shot apical meristems of wheat after ballistic microtargeting", THE PLANT JOURNAL, vol. 4, no. 4, pages 735 - 744 *
GAMBLEY, R.L., ET AL.: "Microprojectile transformation of sugarcane meristems and regeneration of shoots expressing beta-glucuronidase", PLANT CELL REPORTS, vol. 12, pages 343 - 346 *
GOULD, J., ET AL.: "Transformation of Zea mays L. using Agrobacterium tumefaciens and the shoot apex", PLANT PHYSIOLOGY, vol. 95, pages 426 - 434 *
LOWE K., ET AL.: "Germline transformation of maize following manipulation of chimeric shoot meristems.", BIO-TECHNOLOGY (NEW YORK) 13 (7). 1995. 677-682. *
LUSARDI, M.C., ET AL.: "An approach towards genetically engineered cell fate mapping in maize using the Lc gene as a visible marker: transactivation capacity of Lc vectors in differentiated maize cells and microinjection of Lc vectors into somatic embryos and shoot apical meristems", THE PLANT JOURNAL, vol. 5, no. 4, pages 571 - 582 *
MCCABE, D.E., ET AL.: "Stable transformation of soybean (Glycine max) by particle acceleration", BIOTECHNOLOGY, vol. 6, pages 923 - 926 *
MCCABE, D.E., ET AL.: "Transformation of elite cotton cultivars via particle bombardment of meristems", BIOTECHNOLOGY, vol. 11, pages 596 - 598 *
RITALA, A., ET AL.: "Fertile transgenic barley by particle bombardment of immature embryos", PLANT MOLECULAR BIOLOGY, vol. 24, pages 317 - 325 *

Also Published As

Publication number Publication date
JPH10503374A (ja) 1998-03-31
EP0772687A2 (fr) 1997-05-14
NZ293737A (en) 1998-07-28
WO1996004392A2 (fr) 1996-02-15
CA2195206A1 (fr) 1996-02-15
HUT76841A (en) 1997-11-28
MX9700688A (es) 1998-01-31
BR9508341A (pt) 1997-08-12
MX199137B (es) 2000-10-18
AU697373B2 (en) 1998-10-01
AU3624795A (en) 1996-03-04

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