MX9700688A - Plantas transgenicas de cereal. - Google Patents

Plantas transgenicas de cereal.

Info

Publication number
MX9700688A
MX9700688A MX9700688A MX9700688A MX9700688A MX 9700688 A MX9700688 A MX 9700688A MX 9700688 A MX9700688 A MX 9700688A MX 9700688 A MX9700688 A MX 9700688A MX 9700688 A MX9700688 A MX 9700688A
Authority
MX
Mexico
Prior art keywords
meristem
contribute
proembryo
transgenic
sectors
Prior art date
Application number
MX9700688A
Other languages
English (en)
Other versions
MXPA97000688A (es
MX199137B (es
Inventor
Benjamin A Bowen
Keith Lowe
Margot C Ross
Gary A Sandahl
Dwight T Tomes
David D Songstad
William J Gordon-Kamm
Original Assignee
Pionner Hi Bred International
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/483,091 external-priority patent/US5736369A/en
Application filed by Pionner Hi Bred International filed Critical Pionner Hi Bred International
Publication of MXPA97000688A publication Critical patent/MXPA97000688A/es
Publication of MX9700688A publication Critical patent/MX9700688A/es
Publication of MX199137B publication Critical patent/MX199137B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cereal-Derived Products (AREA)
  • Noodles (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

Para obtener una planta de cereal transgénico que se transforma establemente, un meristemo de cereal expuesto se somete a bombardeo biolístico con el fin de apuntar a células del meristemo no diferenciadas para su transformacion. Los embriones inmaduros en el proembrion temprano, proembrion medio, proembrion tardío, etapa coleoptilar transicional o temprana se cultivan para bombardeo biolístico. El tejido del meristemo o células destinadas a contribuir al meristemo, se manipulan después con el fin de alargar los sectores transgénicos, ya sea mediante seleccion y/o por realizar una proliferacion del tejido de los brotes o multiples meristemos por sí mismos. La poblacion de los brotes así obtenida, se tamizo después, por medio de un análisis de enriquecimiento no letal, para identificar cualesquiera sectores quiméricos que contribuirán a la transmision de la línea germinal, quimeras periclinales L2, sin sectores, que por definicion, transmitirán a la progenie. El tiempo incrementado en el cultivo, bajo seleccion, incrementa los prospectos para conversiones sectoriales a periclinales, y también selecciona conversiones de L1 a L2 las cuales, mediante un cambio de posicion contribuyen finalmente a la línea germinal. Los sectores transgénicos también se estabilizan durante el paso de formacion de retoños.
MX9700688A 1994-07-29 1995-07-26 Plantas transgenicas de cereal. MX199137B (es)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US28227094A 1994-07-29 1994-07-29
US282270 1994-07-29
US282,270 1994-07-29
US08483091 1995-06-07
US08/483,091 US5736369A (en) 1994-07-29 1995-06-07 Method for producing transgenic cereal plants
PCT/US1995/008977 WO1996004392A2 (en) 1994-07-29 1995-07-26 Transgenic cereal plants

Publications (3)

Publication Number Publication Date
MXPA97000688A MXPA97000688A (es) 1998-01-01
MX9700688A true MX9700688A (es) 1998-01-31
MX199137B MX199137B (es) 2000-10-18

Family

ID=26961344

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9700688A MX199137B (es) 1994-07-29 1995-07-26 Plantas transgenicas de cereal.

Country Status (9)

Country Link
EP (1) EP0772687A2 (es)
JP (1) JPH10503374A (es)
AU (1) AU697373B2 (es)
BR (1) BR9508341A (es)
CA (1) CA2195206A1 (es)
HU (1) HUT76841A (es)
MX (1) MX199137B (es)
NZ (1) NZ293737A (es)
WO (1) WO1996004392A2 (es)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7102056B1 (en) 1997-04-29 2006-09-05 The Regents Of The University Of California Compositions and methods for plant transformation and regeneration
US6235529B1 (en) 1997-04-29 2001-05-22 The Regents Of The University Of California Compositions and methods for plant transformation and regeneration
EP1017268A1 (en) 1997-09-24 2000-07-12 The Regents Of The University Of California Methods and compositions for transformation of cereals using cultured shoot meristematic tissue
ES2276475T5 (es) 1997-09-30 2014-07-11 The Regents Of The University Of California Producción de proteínas en semillas de plantas
US6153811A (en) * 1997-12-22 2000-11-28 Dekalb Genetics Corporation Method for reduction of transgene copy number
US6096546A (en) * 1998-01-30 2000-08-01 Board Of Trustees, Rutgers, The State University Of New Jersey Methods for recovering polypeptides from plants and portions thereof
US6100092A (en) * 1998-06-15 2000-08-08 Board Of Trustees, Rutgers The State University Of New Jersey Materials and methods for amplifying polynucleotides in plants
AU2001255050B2 (en) * 2000-02-25 2005-07-14 Avestha Gengraine Technologies Pvt. Ltd. A process for generating genetically modified pearl millet through agrobacteriumand biolistic transformation
CN1426476A (zh) * 2000-02-25 2003-06-25 阿维斯塔金格兰技术有限公司 通过农杆菌属和biolistic转化制备遗传修饰过的珍珠稗的方法
US6544789B1 (en) 2000-03-13 2003-04-08 Board Of Trustees, Rutgers, The State University Of New Jersey Phosphorus-controllable recombinant expression of polypeptides in plants
IT1317038B1 (it) * 2000-06-05 2003-05-26 Vitroplant Vivai Di Zuccherell Metodo per la rigenerazione di piante e suoi usi per lamoltiplicazione e/o la trasformazione di piante.
US8212109B2 (en) 2001-07-19 2012-07-03 Monsanto Technology Llc Method for the production of transgenic plants
KR20030010234A (ko) * 2001-07-26 2003-02-05 권오경 차동 신호 검출회로
DE10201637A1 (de) * 2002-01-17 2003-08-07 Univ Albert Ludwigs Freiburg Verfahren zur Herstellung einer Mais-Kalluskultur
US7429691B2 (en) 2002-09-03 2008-09-30 The Regents Of The University Of California Methods and compositions for transformation and regeneration of maize
CA2508279A1 (en) 2002-12-23 2004-07-22 Vical Incorporated Method for freeze-drying nucleic acid/block copolymer/cationic surfactant complexes
US7381422B2 (en) 2002-12-23 2008-06-03 Vical Incorporated Method for producing sterile polynucleotide based medicaments
CA2682095C (en) 2007-03-29 2014-06-03 International Flower Developments Proprietary Limited Method for producing transgenic surface layer chimeric plant
CN105792642A (zh) 2013-10-04 2016-07-20 美国陶氏益农公司 大豆转化方法
EP3115456A4 (en) 2014-03-06 2017-10-25 Riken Plant transformation method
US11499158B2 (en) 2016-05-13 2022-11-15 Kaneka Corporation Method for modifying plant
JP2017205104A (ja) * 2016-05-13 2017-11-24 株式会社カネカ 植物のゲノム編集方法
JP6967217B2 (ja) * 2016-05-13 2021-11-17 株式会社カネカ 形質転換植物の作製方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5177010A (en) * 1986-06-30 1993-01-05 University Of Toledo Process for transforming corn and the products thereof
AU615905B2 (en) * 1987-05-05 1991-10-17 Novartis Ag Plant tissue transformation
FI890917A (fi) * 1988-03-02 1989-09-03 Eidgenoess Tech Hochschule Foerfarande foer framstaellning av transgena vaexter.
FI902635A0 (fi) * 1989-05-29 1990-05-25 Eidgenoess Tech Hochschule Foerfarande foer framstaellning av transgena vaexter.
DE4013099A1 (de) * 1990-04-25 1991-10-31 Hoechst Ag Verfahren zur transformation somatischer embryonen von nutzpflanzen
WO1992020809A1 (en) * 1991-05-15 1992-11-26 Agracetus, Inc. Method of creating a transformed rice plant
US5304719A (en) * 1992-02-03 1994-04-19 Pioneer Hi-Bred International, Inc. Inbred corn line PHT47
FI933682A0 (fi) * 1993-08-20 1993-08-20 Valtion Teknillinen Metod foer att producera transgena saedesvaexter

Also Published As

Publication number Publication date
HUT76841A (en) 1997-11-28
AU3624795A (en) 1996-03-04
CA2195206A1 (en) 1996-02-15
EP0772687A2 (en) 1997-05-14
BR9508341A (pt) 1997-08-12
JPH10503374A (ja) 1998-03-31
AU697373B2 (en) 1998-10-01
WO1996004392A2 (en) 1996-02-15
WO1996004392A3 (en) 1996-03-28
NZ293737A (en) 1998-07-28
MX199137B (es) 2000-10-18

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