CA2092588A1 - Enhanced regeneration system for cereals - Google Patents

Enhanced regeneration system for cereals

Info

Publication number
CA2092588A1
CA2092588A1 CA 2092588 CA2092588A CA2092588A1 CA 2092588 A1 CA2092588 A1 CA 2092588A1 CA 2092588 CA2092588 CA 2092588 CA 2092588 A CA2092588 A CA 2092588A CA 2092588 A1 CA2092588 A1 CA 2092588A1
Authority
CA
Canada
Prior art keywords
cereals
regeneration system
plantlets
enhanced regeneration
embryos
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA 2092588
Other languages
French (fr)
Other versions
CA2092588C (en
Inventor
Narender S. Nehra
Kutty K. Kartha
Ravindra N. Chibbar
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Research Council of Canada
Original Assignee
Narender S. Nehra
Kutty K. Kartha
Ravindra N. Chibbar
National Research Council Of Canada
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Narender S. Nehra, Kutty K. Kartha, Ravindra N. Chibbar, National Research Council Of Canada filed Critical Narender S. Nehra
Priority to CA002092588A priority Critical patent/CA2092588C/en
Publication of CA2092588A1 publication Critical patent/CA2092588A1/en
Application granted granted Critical
Publication of CA2092588C publication Critical patent/CA2092588C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

39 Whole scutella are isolated from immature zygotic embryos of cereal plants and cultivated, in the absence of the zygotic embryo axis, to produce somatic embryos, which in turn are converted into plantlets. The scutellar cells optionally are transformed with foreign DNA so that at least some of the resulting plantlets are transgenic or chimeric. The regeneration is much more rapid than with conventional methods.
CA002092588A 1993-03-26 1993-03-26 Enhanced regeneration system for cereals Expired - Fee Related CA2092588C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002092588A CA2092588C (en) 1993-03-26 1993-03-26 Enhanced regeneration system for cereals

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA002092588A CA2092588C (en) 1993-03-26 1993-03-26 Enhanced regeneration system for cereals

Publications (2)

Publication Number Publication Date
CA2092588A1 true CA2092588A1 (en) 1994-09-27
CA2092588C CA2092588C (en) 2008-07-08

Family

ID=4151356

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002092588A Expired - Fee Related CA2092588C (en) 1993-03-26 1993-03-26 Enhanced regeneration system for cereals

Country Status (1)

Country Link
CA (1) CA2092588C (en)

Cited By (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002037955A1 (en) 2000-11-09 2002-05-16 Commonwealth Scientific And Industrial Research Organisation Barley with reduced ssii activity and starch containing products with a reduced amylopectin content
WO2008025068A1 (en) 2006-08-29 2008-03-06 Commonwealth Scientific And Industrial Research Organisation Synthesis of fatty acids
WO2009021285A1 (en) 2007-08-13 2009-02-19 Commonwealth Scientific And Industrial Research Organisation Barley with low levels of hordein
WO2009067751A1 (en) 2007-11-27 2009-06-04 Commonwealth Scientific And Industrial Research Organisation Plants with modified starch metabolism
WO2009129583A1 (en) 2008-04-25 2009-10-29 Commonwealth Scientific Industrial Research Organisation Recombinant cells and methods for hydroxylating fatty acids
WO2010057246A1 (en) 2008-11-18 2010-05-27 Commonwealth Scientific Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
WO2012000026A1 (en) 2010-06-28 2012-01-05 Commonwealth Scientific And Industrial Research Organisation Methods of producing lipids
US8278063B2 (en) 2007-06-29 2012-10-02 Commonwealth Scientific And Industrial Research Organisation Methods for degrading toxic compounds
WO2013096991A1 (en) 2011-12-27 2013-07-04 Commonwealth Scientific And Industrial Research Organisation Production of dihydrosterculic acid and derivatives thereof
WO2013096993A1 (en) 2011-12-27 2013-07-04 Commonwealth Scientific And Industrial Research Organisation Processes for producing lipids
WO2013096992A1 (en) 2011-12-27 2013-07-04 Commonwealth Scientific And Industrial Reserach Organisation Simultaneous gene silencing and supressing gene silencing in the same cell
US8536429B2 (en) 2006-07-12 2013-09-17 Commonwealth Scientific And Industrial Research Organisation Polynucleotides encoding a NAX2 polypeptide and methods for enhancing salinity tolerance in plants
WO2013159149A1 (en) 2012-04-25 2013-10-31 Commonwealth Scientific And Industrial Research Organisation High oleic acid oils
US8581038B2 (en) 2008-08-25 2013-11-12 Grains Research And Development Corporation Resistance genes
WO2015024066A1 (en) 2013-08-21 2015-02-26 Commonwealth Scientific And Industrial Research Organisation Rust resistance gene
US9307708B2 (en) 2014-03-07 2016-04-12 Pioneer Hi-Bred International Inc Systems for extracting monocot embryos
US9718759B2 (en) 2013-12-18 2017-08-01 Commonwealth Scientific And Industrial Research Organisation Lipid comprising docosapentaenoic acid
US9796990B2 (en) 2011-07-20 2017-10-24 Commonwealth Scientific And Industrial Research Organization Enzymes for degrading organophosphates
US9932289B2 (en) 2012-06-15 2018-04-03 Commonwealth Scientific And Industrial Research Ogranisation Process for producing ethyl esters of polyunsaturated fatty acids
US10005713B2 (en) 2014-06-27 2018-06-26 Commonwealth Scientific And Industrial Research Organisation Lipid compositions comprising triacylglycerol with long-chain polyunsaturated fatty acids at the sn-2 position
US10849345B2 (en) 2013-06-13 2020-12-01 Commonwealth Scientific And Industrial Research Organisation Barley with very low levels of hordeins
US10941428B2 (en) 2018-09-12 2021-03-09 Universidad Politécnica de Madrid Reagents and methods for the expression of an active NifB protein and uses thereof

Cited By (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2342970A2 (en) 2000-11-09 2011-07-13 Commonwealth Scientific and Industrial Research Organisation Barley with reduced SSII activity and starch and starch containing products with a reduced amylopectin content
WO2002037955A1 (en) 2000-11-09 2002-05-16 Commonwealth Scientific And Industrial Research Organisation Barley with reduced ssii activity and starch containing products with a reduced amylopectin content
US8536429B2 (en) 2006-07-12 2013-09-17 Commonwealth Scientific And Industrial Research Organisation Polynucleotides encoding a NAX2 polypeptide and methods for enhancing salinity tolerance in plants
WO2008025068A1 (en) 2006-08-29 2008-03-06 Commonwealth Scientific And Industrial Research Organisation Synthesis of fatty acids
US8278063B2 (en) 2007-06-29 2012-10-02 Commonwealth Scientific And Industrial Research Organisation Methods for degrading toxic compounds
US11326134B2 (en) 2007-08-13 2022-05-10 Commonwealth Scientific And Industrial Research Organisation Barley with low levels of hordeins
US10501712B2 (en) 2007-08-13 2019-12-10 Commonwealth Scientific And Industrial Research Organisation Barley with low levels of hordeins
US9133427B2 (en) 2007-08-13 2015-09-15 Commonwealth Scientific And Industrial Research Organisation Barley with low levels of hordeins
WO2009021285A1 (en) 2007-08-13 2009-02-19 Commonwealth Scientific And Industrial Research Organisation Barley with low levels of hordein
US8642846B2 (en) 2007-08-13 2014-02-04 Commonwealth Scientific And Industrial Research Organisation Barley with low levels of hordeins
WO2009067751A1 (en) 2007-11-27 2009-06-04 Commonwealth Scientific And Industrial Research Organisation Plants with modified starch metabolism
WO2009129583A1 (en) 2008-04-25 2009-10-29 Commonwealth Scientific Industrial Research Organisation Recombinant cells and methods for hydroxylating fatty acids
US8581038B2 (en) 2008-08-25 2013-11-12 Grains Research And Development Corporation Resistance genes
US9115370B2 (en) 2008-08-25 2015-08-25 Grains Research And Development Corporation Resistance genes
EP3260544A1 (en) 2008-11-18 2017-12-27 Commonwealth Scientific and Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
US9976107B2 (en) 2008-11-18 2018-05-22 Commonwealth Scientific And Industrial Research Organisation Enzymes and methods for producing ω-3 fatty acids
WO2010057246A1 (en) 2008-11-18 2010-05-27 Commonwealth Scientific Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
US10648046B2 (en) 2008-11-18 2020-05-12 Commonwealth Scientific And Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
US9994792B2 (en) 2008-11-18 2018-06-12 Commonwealth Scientific And Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
US11976287B2 (en) 2008-11-18 2024-05-07 Commonwealth Scientific And Industrial Research Organisation Enzymes and methods for producing ω-3 fatty acids
US9938486B2 (en) 2008-11-18 2018-04-10 Commonwealth Scientific And Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
WO2012000026A1 (en) 2010-06-28 2012-01-05 Commonwealth Scientific And Industrial Research Organisation Methods of producing lipids
US9796990B2 (en) 2011-07-20 2017-10-24 Commonwealth Scientific And Industrial Research Organization Enzymes for degrading organophosphates
WO2013096991A1 (en) 2011-12-27 2013-07-04 Commonwealth Scientific And Industrial Research Organisation Production of dihydrosterculic acid and derivatives thereof
WO2013096993A1 (en) 2011-12-27 2013-07-04 Commonwealth Scientific And Industrial Research Organisation Processes for producing lipids
WO2013096992A1 (en) 2011-12-27 2013-07-04 Commonwealth Scientific And Industrial Reserach Organisation Simultaneous gene silencing and supressing gene silencing in the same cell
WO2013159149A1 (en) 2012-04-25 2013-10-31 Commonwealth Scientific And Industrial Research Organisation High oleic acid oils
US10335386B2 (en) 2012-06-15 2019-07-02 Commonwealth Scientific And Industrial Research Organisation Lipid comprising polyunsaturated fatty acids
US9932289B2 (en) 2012-06-15 2018-04-03 Commonwealth Scientific And Industrial Research Ogranisation Process for producing ethyl esters of polyunsaturated fatty acids
US10849345B2 (en) 2013-06-13 2020-12-01 Commonwealth Scientific And Industrial Research Organisation Barley with very low levels of hordeins
EP4001300A1 (en) 2013-08-21 2022-05-25 Commonwealth Scientific and Industrial Research Organisation Rust resistance gene
WO2015024066A1 (en) 2013-08-21 2015-02-26 Commonwealth Scientific And Industrial Research Organisation Rust resistance gene
US10125084B2 (en) 2013-12-18 2018-11-13 Commonwealth Scientific And Industrial Research Organisation Lipid comprising docosapentaenoic acid
US10190073B2 (en) 2013-12-18 2019-01-29 Commonwealth Scientific And Industrial Research Organisation Lipid comprising long chain polyunsaturated fatty acids
US10800729B2 (en) 2013-12-18 2020-10-13 Commonwealth Scientific And Industrial Research Organisation Lipid comprising long chain polyunsaturated fatty acids
US9725399B2 (en) 2013-12-18 2017-08-08 Commonwealth Scientific And Industrial Research Organisation Lipid comprising long chain polyunsaturated fatty acids
US9718759B2 (en) 2013-12-18 2017-08-01 Commonwealth Scientific And Industrial Research Organisation Lipid comprising docosapentaenoic acid
US11623911B2 (en) 2013-12-18 2023-04-11 Commonwealth Scientific And Industrial Research Organisation Lipid comprising docosapentaenoic acid
US9320210B2 (en) 2014-03-07 2016-04-26 Pioneer Hi Bred Internatonal Inc Methods of extracting monocot embryos
US9307708B2 (en) 2014-03-07 2016-04-12 Pioneer Hi-Bred International Inc Systems for extracting monocot embryos
US10005713B2 (en) 2014-06-27 2018-06-26 Commonwealth Scientific And Industrial Research Organisation Lipid compositions comprising triacylglycerol with long-chain polyunsaturated fatty acids at the sn-2 position
US10793507B2 (en) 2014-06-27 2020-10-06 Commonwealth Scientific And Industrial Research Organisation Lipid compositions comprising triacylglycerol with long-chain polyunsaturated fatty acids at the SN-2 position
US10941428B2 (en) 2018-09-12 2021-03-09 Universidad Politécnica de Madrid Reagents and methods for the expression of an active NifB protein and uses thereof

Also Published As

Publication number Publication date
CA2092588C (en) 2008-07-08

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