CA1119166A - Procede de recuperation des proteines du lactoserum - Google Patents
Procede de recuperation des proteines du lactoserumInfo
- Publication number
- CA1119166A CA1119166A CA000327208A CA327208A CA1119166A CA 1119166 A CA1119166 A CA 1119166A CA 000327208 A CA000327208 A CA 000327208A CA 327208 A CA327208 A CA 327208A CA 1119166 A CA1119166 A CA 1119166A
- Authority
- CA
- Canada
- Prior art keywords
- whey
- proteins
- fact
- terrified
- charac
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 59
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 59
- 108010046377 Whey Proteins Proteins 0.000 title claims abstract description 46
- 239000005862 Whey Substances 0.000 title claims abstract description 45
- 102000007544 Whey Proteins Human genes 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 34
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 29
- 238000004925 denaturation Methods 0.000 claims abstract description 19
- 238000010438 heat treatment Methods 0.000 claims abstract description 15
- 230000036425 denaturation Effects 0.000 claims abstract description 11
- 239000000470 constituent Substances 0.000 claims abstract description 8
- 239000012465 retentate Substances 0.000 claims description 26
- 238000010790 dilution Methods 0.000 claims description 10
- 239000012895 dilution Substances 0.000 claims description 10
- 210000002966 serum Anatomy 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims 1
- 235000018102 proteins Nutrition 0.000 abstract 1
- 235000021119 whey protein Nutrition 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 18
- 239000000203 mixture Substances 0.000 description 15
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 11
- 239000008101 lactose Substances 0.000 description 11
- 239000000047 product Substances 0.000 description 11
- 229910052757 nitrogen Inorganic materials 0.000 description 9
- 235000013351 cheese Nutrition 0.000 description 8
- 238000010793 Steam injection (oil industry) Methods 0.000 description 7
- 239000002253 acid Substances 0.000 description 6
- 235000005911 diet Nutrition 0.000 description 6
- 229910052500 inorganic mineral Inorganic materials 0.000 description 6
- 239000011707 mineral Substances 0.000 description 6
- 239000006071 cream Substances 0.000 description 5
- 235000013365 dairy product Nutrition 0.000 description 5
- 150000003839 salts Chemical class 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 4
- 235000009508 confectionery Nutrition 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 235000013336 milk Nutrition 0.000 description 4
- 239000008267 milk Substances 0.000 description 4
- 210000004080 milk Anatomy 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 102000004506 Blood Proteins Human genes 0.000 description 3
- 108010017384 Blood Proteins Proteins 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 230000037213 diet Effects 0.000 description 3
- 230000000378 dietary effect Effects 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 235000013312 flour Nutrition 0.000 description 2
- 239000004519 grease Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- 241000237074 Centris Species 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 101000937129 Drosophila melanogaster Cadherin-related tumor suppressor Proteins 0.000 description 1
- 102000004407 Lactalbumin Human genes 0.000 description 1
- 108090000942 Lactalbumin Proteins 0.000 description 1
- 241000186167 Lophira alata Species 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 239000005913 Maltodextrin Substances 0.000 description 1
- 240000002129 Malva sylvestris Species 0.000 description 1
- 235000006770 Malva sylvestris Nutrition 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000000721 bacterilogical effect Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 235000013877 carbamide Nutrition 0.000 description 1
- 229910052729 chemical element Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000001805 chlorine compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000005115 demineralization Methods 0.000 description 1
- 230000002328 demineralizing effect Effects 0.000 description 1
- 238000011026 diafiltration Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 235000013861 fat-free Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000003505 heat denaturation Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 235000015250 liver sausages Nutrition 0.000 description 1
- 229940035034 maltodextrin Drugs 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 238000009928 pasteurization Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000001012 protector Effects 0.000 description 1
- NXLOLUFNDSBYTP-UHFFFAOYSA-N retene Chemical compound C1=CC=C2C3=CC=C(C(C)C)C=C3C=CC2=C1C NXLOLUFNDSBYTP-UHFFFAOYSA-N 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 150000003672 ureas Chemical class 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/20—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from milk, e.g. casein; from whey
- A23J1/205—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from milk, e.g. casein; from whey from whey, e.g. lactalbumine
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C19/00—Cheese; Cheese preparations; Making thereof
- A23C19/02—Making cheese curd
- A23C19/05—Treating milk before coagulation; Separating whey from curd
- A23C19/053—Enrichment of milk with whey, whey components, substances recovered from separated whey, isolated or concentrated proteins from milk
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/14—Milk preparations; Milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment
- A23C9/142—Milk preparations; Milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment by dialysis, reverse osmosis or ultrafiltration
- A23C9/1425—Milk preparations; Milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment by dialysis, reverse osmosis or ultrafiltration by ultrafiltration, microfiltration or diafiltration of whey, e.g. treatment of the UF permeate
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/827—Proteins from mammals or birds
- Y10S530/833—Whey; cheese
Landscapes
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Water Supply & Treatment (AREA)
- Biochemistry (AREA)
- Dairy Products (AREA)
- Peptides Or Proteins (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CH513478A CH630243A5 (fr) | 1978-05-11 | 1978-05-11 | Procede de recuperation des proteines du lactoserum. |
CH5134/78-6 | 1978-05-11 |
Publications (1)
Publication Number | Publication Date |
---|---|
CA1119166A true CA1119166A (fr) | 1982-03-02 |
Family
ID=4288510
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA000327208A Expired CA1119166A (fr) | 1978-05-11 | 1979-05-08 | Procede de recuperation des proteines du lactoserum |
Country Status (17)
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2459619B1 (fr) * | 1979-06-26 | 1983-07-29 | Agronomique Inst Nat Rech | Procede pour l'obtention a partir de lactoserum, d'un produit enrichi en alpha-lactalbumine et applications dudit procede |
FR2483748A1 (fr) * | 1980-06-06 | 1981-12-11 | Corning Glass Works | Procede d'hydrolyse du lactose contenu dans les lactoserums a l'aide de lactase immobilisee |
AT376549B (de) * | 1982-05-17 | 1984-11-26 | Molkereigenossenschaft St Joha | Verfahren zur herstellung eines eiweisshaltigen nahrungs- oder futtermittels |
US5102681A (en) * | 1984-05-04 | 1992-04-07 | John Labatt Limited/John Labatt Limitee | Reduced fat salad dressing |
US4734287A (en) * | 1986-06-20 | 1988-03-29 | John Labatt Limited | Protein product base |
US5098728A (en) * | 1989-06-16 | 1992-03-24 | John Labatt Limited/John Labbat Limitee | Reduced fat food product |
US5139811A (en) * | 1984-05-04 | 1992-08-18 | John Labatt Limited | Viscous salad dressing |
US4961953A (en) * | 1986-06-20 | 1990-10-09 | John Labatt Limited/John Labatt Limitee | Fat emulating protein products and process |
DE3432718C1 (de) * | 1984-09-06 | 1986-05-22 | Biotest Pharma GmbH, 6000 Frankfurt | Verfahren zur Herstellung einer Loesung von Milch- und/oder Kolostralimmunglobulinen |
NL8403701A (nl) * | 1984-12-05 | 1986-07-01 | Holland Melkunie | Werkwijze voor het verwerken van bij ultrafiltratie van wei verkregen produkten in zuivelprodukten alsmede zuivelprodukten, die uit wei afkomstige produkten bevatten. |
US5096730A (en) * | 1986-06-20 | 1992-03-17 | John Labatt Limited/John Labatt Limitee | Reduced fat sour cream |
US4749783A (en) * | 1986-07-11 | 1988-06-07 | Miles Laboratories, Inc. | Viral inactivation and purification of active proteins |
GB8628104D0 (en) * | 1986-11-25 | 1986-12-31 | Connaught Lab | Pasteurization of immunoglobin solutions |
JPH0279940A (ja) * | 1988-06-16 | 1990-03-20 | Unilever Nv | 食用可塑性組成物 |
US5096731A (en) * | 1989-06-16 | 1992-03-17 | John Labatt Limited/John Labatt Limitee | Reduced fat yogurt |
DE4002204A1 (de) * | 1990-01-26 | 1991-08-01 | Westfalen Milchwerke | Diaetetisches nahrungsmittel fuer patienten mit niereninsuffizienz |
US5080921A (en) * | 1990-02-05 | 1992-01-14 | Pfizer Inc. | Low calorie fat substitute |
NL9001692A (nl) * | 1990-04-26 | 1991-11-18 | Cooeperatieve Melkproductenbed | Werkwijze voor de bereiding van een olie-in-water emulsie. |
DK0468560T4 (da) * | 1990-07-23 | 1998-11-23 | Unilever Plc | Proteindispersioner i næringsmiddelprodukter |
CA2046741C (en) * | 1991-05-16 | 1999-06-29 | Yashavantkumar Jayasinh Asher | Whey protein concentrate and its use in ice cream |
AU2394792A (en) * | 1991-08-01 | 1993-03-02 | Ault Foods Limited | Whey and ice cream products and processes |
CA2094570A1 (en) * | 1992-05-27 | 1993-11-28 | Jerome F. Trumbetas | Enzymatic protein process and product |
US5707678A (en) * | 1995-04-12 | 1998-01-13 | Galagen Inc. | Method for microfiltration of milk or colostral whey |
US5670196A (en) * | 1995-04-12 | 1997-09-23 | Galagen Inc. | Method for microfiltration of milk or colostral whey |
DE19906379B4 (de) * | 1999-02-16 | 2006-05-18 | Huss, Manfred | Herstellung eines aggregierten Molkenproteinprodukts |
DE50100977D1 (de) * | 2001-10-23 | 2003-12-18 | Mueller Alois Molkerei | Verfahren zur Herstellung von Molkenproteinaggregaten aus Sauermolke |
US20030166866A1 (en) * | 2002-01-28 | 2003-09-04 | Land O' Lakes, Inc. | Method of processing a proteinaceous material to recover K-casein macropeptide and polymers of a-lactalbumin and B-lactoglobulin |
MX2007003633A (es) * | 2004-09-29 | 2007-06-13 | Nestec Sa | Proteina globular activada y su uso en composiciones comestibles. |
US7687095B2 (en) * | 2005-09-30 | 2010-03-30 | Kraft Foods Global Brands Llc | High moisture, low fat cream cheese with maintained product quality and method for making same |
US20070134396A1 (en) * | 2005-12-13 | 2007-06-14 | Kraft Foods Holding, Inc. | Modified Whey Protein For Low Casein Processed Cheese |
US20090169690A1 (en) * | 2007-12-28 | 2009-07-02 | Yinqing Ma | Increasing the Firmness of Process Cheese by Utilizing Ingredient Synergism |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2602746A (en) * | 1949-07-19 | 1952-07-08 | Western Condensing Co | Milk product method of manufacture |
US3066133A (en) * | 1959-04-15 | 1962-11-27 | Laval Separator Co De | Removal of protein from cheese whey |
FR1555757A (US20020051482A1-20020502-M00057.png) * | 1967-11-30 | 1969-01-31 | ||
US3963837A (en) * | 1969-07-18 | 1976-06-15 | Maubois Jean Louis Joseph | Preparation of cheese from ultrafiltered milk |
US3914435A (en) * | 1969-07-18 | 1975-10-21 | Agronomique Inst Nat Rech | Manufacture of cheese from ultrafiltered milk |
DE2138277C2 (de) * | 1971-07-30 | 1982-09-16 | Meggle Milchindustrie Gmbh & Co Kg, 8094 Reitmehring | Verfahren zur Herstellung eines Molkeeiweißkonzentrates |
DE2155696B2 (de) * | 1971-11-09 | 1974-02-28 | Molkerei Josef Anton Meggle Milchindustrie, 8094 Reitmehring | Verfahren zur Ultrafiltration von Molke |
CH556143A (fr) * | 1972-09-11 | 1974-11-29 | Nestle Sa | Procede de preparation d'une fraction soluble des proteines du petit lait. |
FR2288477A1 (fr) * | 1974-10-22 | 1976-05-21 | Manche Union Coop Agr Laiti | Procede de production d'acide sialique et de glycoproteines a partir de lactoserum de fromagerie |
FR2288473A1 (fr) * | 1974-10-22 | 1976-05-21 | Manche Union Coop Agr Laiti | Procede de traitement du lactoserum de fromagerie, notamment en vue de l'extraction de glycoproteides et d'acide sialique |
-
1978
- 1978-05-11 CH CH513478A patent/CH630243A5/fr not_active IP Right Cessation
-
1979
- 1979-04-09 DE DE2914301A patent/DE2914301C2/de not_active Expired
- 1979-04-12 NZ NZ190180A patent/NZ190180A/xx unknown
- 1979-04-20 AU AU46301/79A patent/AU519300B2/en not_active Expired
- 1979-04-24 ZA ZA791958A patent/ZA791958B/xx unknown
- 1979-04-26 NL NLAANVRAGE7903295,A patent/NL190308C/xx not_active IP Right Cessation
- 1979-05-03 GB GB7915368A patent/GB2020667B/en not_active Expired
- 1979-05-07 FI FI791461A patent/FI64044C/fi not_active IP Right Cessation
- 1979-05-07 US US06/036,427 patent/US4265924A/en not_active Expired - Lifetime
- 1979-05-08 AR AR276443A patent/AR223486A1/es active
- 1979-05-08 CA CA000327208A patent/CA1119166A/fr not_active Expired
- 1979-05-08 SE SE7904010A patent/SE445704B/sv not_active IP Right Cessation
- 1979-05-09 MX MX797966U patent/MX5556E/es unknown
- 1979-05-10 AT AT0348779A patent/AT363767B/de not_active IP Right Cessation
- 1979-05-10 ES ES480418A patent/ES480418A1/es not_active Expired
- 1979-05-10 IT IT48995/79A patent/IT1164681B/it active
- 1979-08-08 IE IE1260/79A patent/IE48363B1/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
SE7904010L (sv) | 1979-11-12 |
AR223486A1 (es) | 1981-08-31 |
GB2020667A (en) | 1979-11-21 |
DE2914301A1 (de) | 1979-11-15 |
CH630243A5 (fr) | 1982-06-15 |
FI791461A (fi) | 1979-11-12 |
NL190308C (nl) | 1994-01-17 |
FI64044C (fi) | 1983-10-10 |
FI64044B (fi) | 1983-06-30 |
ATA348779A (de) | 1981-01-15 |
US4265924A (en) | 1981-05-05 |
SE445704B (sv) | 1986-07-14 |
AT363767B (de) | 1981-08-25 |
MX5556E (es) | 1983-10-06 |
DE2914301C2 (de) | 1987-01-02 |
IT1164681B (it) | 1987-04-15 |
NL190308B (nl) | 1993-08-16 |
ZA791958B (en) | 1980-05-28 |
IE791260L (en) | 1979-11-11 |
AU519300B2 (en) | 1981-11-19 |
NZ190180A (en) | 1980-11-28 |
IT7948995A0 (it) | 1979-05-10 |
GB2020667B (en) | 1982-09-02 |
IE48363B1 (en) | 1984-12-26 |
ES480418A1 (es) | 1979-12-16 |
NL7903295A (nl) | 1979-11-13 |
AU4630179A (en) | 1979-11-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
MKEX | Expiry |