BRPI0806252B8 - molécula de ácido nucléico de promotor, vetor recombinante, célula de hospedeiro e método de expressão de gene alvo - Google Patents

molécula de ácido nucléico de promotor, vetor recombinante, célula de hospedeiro e método de expressão de gene alvo

Info

Publication number
BRPI0806252B8
BRPI0806252B8 BRPI0806252A BRPI0806252A BRPI0806252B8 BR PI0806252 B8 BRPI0806252 B8 BR PI0806252B8 BR PI0806252 A BRPI0806252 A BR PI0806252A BR PI0806252 A BRPI0806252 A BR PI0806252A BR PI0806252 B8 BRPI0806252 B8 BR PI0806252B8
Authority
BR
Brazil
Prior art keywords
host cell
nucleic acid
acid molecule
recombinant vector
gene expression
Prior art date
Application number
BRPI0806252A
Other languages
English (en)
Inventor
Jang Jae-Woo
Jo Lim Sang
Yeon Rah So
Lee Sun-Young
Hoon Park Young
Original Assignee
Cj Cheiljedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cj Cheiljedang Corp filed Critical Cj Cheiljedang Corp
Publication of BRPI0806252A2 publication Critical patent/BRPI0806252A2/pt
Publication of BRPI0806252B1 publication Critical patent/BRPI0806252B1/pt
Publication of BRPI0806252B8 publication Critical patent/BRPI0806252B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

molécula de ácido nucléico de promotor, vetor recombinante, célula de hospedeiro e método de expressão de gene alvo. são proporcionados uma molécula inovativa de ácido nucléico de promotor que tem uma sequência de nucleotideo de seq id no: 1 ou 2 derivada de corynebacterium glutamicum, um vetor recombinante que compreende o promotor, uma célula de hospedeiro trans- formada com o vetor e um método de expressar genes de interesse usando a célula de hospedeiro.
BRPI0806252A 2007-01-15 2008-01-15 molécula de ácido nucléico de promotor, vetor recombinante, célula de hospedeiro e método de expressão de gene alvo BRPI0806252B8 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020070004386A KR100789274B1 (ko) 2007-01-15 2007-01-15 코리네박테리움 글루타미쿰에서 유래한 신규한 프로모터핵산 분자, 그 프로모터를 포함하는 재조합 벡터, 그재조합 벡터를 포함하는 숙주 세포 및 그 숙주 세포를이용하여 유전자를 발현하는 방법
PCT/KR2008/000246 WO2008088158A1 (en) 2007-01-15 2008-01-15 Novel promoter nucleic acid molecule derived from corynebacterium glutamicum, recombinant vector comprising the promoter, host cell comprising the recombinant vector and method of expressing gene using the host cell

Publications (3)

Publication Number Publication Date
BRPI0806252A2 BRPI0806252A2 (pt) 2011-08-30
BRPI0806252B1 BRPI0806252B1 (pt) 2016-11-29
BRPI0806252B8 true BRPI0806252B8 (pt) 2017-05-02

Family

ID=39216004

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0806252A BRPI0806252B8 (pt) 2007-01-15 2008-01-15 molécula de ácido nucléico de promotor, vetor recombinante, célula de hospedeiro e método de expressão de gene alvo

Country Status (7)

Country Link
US (1) US8426195B2 (pt)
JP (1) JP5266252B2 (pt)
KR (1) KR100789274B1 (pt)
CN (1) CN101605890B (pt)
BR (1) BRPI0806252B8 (pt)
DE (1) DE112008000181T9 (pt)
WO (1) WO2008088158A1 (pt)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101072708B1 (ko) * 2008-12-17 2011-10-11 씨제이제일제당 (주) ５＇－크산틸산 생산능이 향상된 미생물 및 이를 이용한 ５＇－크산틸산의 생산방법
KR101072720B1 (ko) * 2008-12-17 2011-10-11 씨제이제일제당 (주) ５＇－구아닐산 생산능이 향상된 미생물 및 이를 이용한 ５＇－구아닐산의 생산방법
KR101226384B1 (ko) * 2010-03-05 2013-01-25 씨제이제일제당 (주) 개량된 프로모터 및 이를 이용한 ｌ-라이신의 생산 방법
DE102010003419B4 (de) 2010-03-30 2019-09-12 Evonik Degussa Gmbh Verfahren zur fermentativen Herstellung von L-Ornithin
WO2012047076A2 (ko) 2010-10-08 2012-04-12 한국생명공학연구원 파라고무나무 유래의 라텍스 분비 조직 특이적 ｓｒｐｐ 프로모터 및 이의 용도
US8846367B2 (en) * 2010-11-18 2014-09-30 Green Phenol Development Co., Ltd. Coryneform bacterium transformant and process for producing phenol using the same
KR20140066553A (ko) 2012-11-23 2014-06-02 삼성전자주식회사 코리네박테리움 속 균주의 신규 프로모터
KR20140095850A (ko) 2013-01-25 2014-08-04 삼성전자주식회사 코리네박테리움과 대장균용 셔틀벡터
KR101677368B1 (ko) 2015-04-02 2016-11-18 씨제이제일제당 (주) 신규 프로모터 및 이의 용도
KR20180084756A (ko) 2015-12-07 2018-07-25 지머젠 인코포레이티드 코리네박테리움 글루타미컴으로부터의 프로모터
ES2856755T3 (es) * 2015-12-07 2021-09-28 Zymergen Inc Promotores de Corynebacterium glutamicum
EP3635117A2 (en) * 2017-06-07 2020-04-15 Zymergen, Inc. Promoters from corynebacterium glutamicum and uses thereof in regulating ancillary gene expression
KR101925930B1 (ko) * 2017-07-24 2019-02-26 씨제이제일제당 주식회사 Ｌ-라이신을 생산하는 미생물 및 이를 이용한 ｌ-라이신의 생산 방법
KR102016050B1 (ko) * 2018-03-20 2019-08-29 씨제이제일제당 주식회사 신규한 프로모터 및 이의 용도
WO2022152036A1 (zh) * 2021-01-12 2022-07-21 中国科学院天津工业生物技术研究所 具有启动子活性的多核苷酸及其在生产目标化合物中的用途
CN115322989A (zh) * 2021-05-10 2022-11-11 中国科学院天津工业生物技术研究所 具有启动子活性的多核苷酸及其用途和生产目标化合物的方法
CN112877352B (zh) * 2021-02-08 2023-03-21 华南理工大学 一种适用于谷氨酸棒状杆菌的cumate诱导系统及该诱导系统构建的质粒载体和应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19931314A1 (de) * 1999-07-07 2001-01-11 Degussa L-Lysin produzierende coryneforme Bakterien und Verfahren zur Herstellung von Lysin
JP4623825B2 (ja) * 1999-12-16 2011-02-02 協和発酵バイオ株式会社 新規ポリヌクレオチド
JP2003265177A (ja) 2002-03-13 2003-09-24 Gekkeikan Sake Co Ltd タンパク質の高発現システム
CA2543605A1 (en) 2003-08-27 2005-03-10 Orf Liftaekni Hf Enhancing accumulation of heterologous polypeptides in plant seeds through targeted suppression of endogenous storage proteins
DE102004035065A1 (de) * 2004-07-20 2006-02-16 Basf Ag P-ET-TS-Expressionseinheiten
KR100620092B1 (ko) 2004-12-16 2006-09-08 씨제이 주식회사 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
JP2006214933A (ja) 2005-02-04 2006-08-17 Seiko Epson Corp 測位システム、端末装置、端末装置の制御方法、端末装置の制御プログラム、端末装置の制御プログラムを記録したコンピュータ読み取り可能な記録媒体
JP4700395B2 (ja) 2005-04-13 2011-06-15 株式会社豊田中央研究所 酸性条件下で使用するためのプロモーター及びその利用
EP3170889A1 (en) 2006-09-15 2017-05-24 CJ Cheiljedang Corporation A corynebacteria having enhanced l-lysine productivity and a method of producing l-lysine using the same

Also Published As

Publication number Publication date
CN101605890B (zh) 2013-05-01
CN101605890A (zh) 2009-12-16
KR100789274B1 (ko) 2008-01-02
JP5266252B2 (ja) 2013-08-21
US20100261256A1 (en) 2010-10-14
DE112008000181T5 (de) 2009-12-10
WO2008088158A1 (en) 2008-07-24
JP2010515453A (ja) 2010-05-13
BRPI0806252B1 (pt) 2016-11-29
BRPI0806252A2 (pt) 2011-08-30
US8426195B2 (en) 2013-04-23
DE112008000181T9 (de) 2010-04-08

Similar Documents

Publication Publication Date Title
BRPI0806252B8 (pt) molécula de ácido nucléico de promotor, vetor recombinante, célula de hospedeiro e método de expressão de gene alvo
CN108690845B (zh) 基因组编辑系统和方法
BRPI0814781A8 (pt) ''proteína, sequência de ácido nucleico, método para a produção de ácido itacônico, vetor, célula hospedeira, e, uso de um ácido cis-aconítico descarboxilase''
EA201991442A1 (ru) ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9
Mayaux et al. Structural and transcriptional evidence for related thrS and infC expression.
NZ592082A (en) Promoters for recombinant viral expression
IL201963A (en) Vectors for multi-gene expression, host cell and pharmacy preparation containing and using them
DE50311850D1 (de) Konstrukte und verfahren zur regulation der genexpression
BRPI0519344A2 (pt) promotor induzÍvel por l-lisina
MX2020003092A (es) Vectores de adn no integrantes para la modificacion genetica de celulas.
Peacock et al. Amino acid–dependent stability of the acyl linkage in aminoacyl-tRNA
CR20200236A (es) Synpiii, un promotor para la expresión específica de genes en el epitelio pigmentario retiniano
BR9804896A (pt) Dna isolado e purificado, célula hospedeira, vetor, polinucleotídeo quimérico, plasmídeo e processo para aumentar a transcrição de um gene de interesse em uma célula hospedeira
PE20130323A1 (es) Un microorganismo que produce un amino de la familia de acidos l-glutamicos y el metodo que permite producir el amino acido
NZ609903A (en) Expression vector for high level expression of recombinant proteins
MY156855A (en) Novel regulatory elements
NZ580378A (en) Recombinant expression vector elements (reves) for enhancing expression of recombinant proteins in host cells
GB201201178D0 (en) Novel enzymes
ES2737098T3 (es) Proteína de exportación de O-fosfoserina novedosa y procedimiento para producir O-fosfoserina usando la misma
WO2009025470A3 (en) Novel corynebacterium glutamicum promoter
Graham et al. Convergent evolution of coenzyme M biosynthesis in the Methanosarcinales: cysteate synthase evolved from an ancestral threonine synthase
MX2010009071A (es) Peptidos aislados que tienen actividad inhibitoria de fosfolipasa.
BRPI0820004A8 (pt) Molécula de ácido nucléico isolada, cassete de expressão, vetor, método para obter uma célula vegetal, método para obter uma planta, método para expressar um polinucleotídeo em uma planta ou uma célula vegetal, método para expressar um polinucleotídeo preferencialmente em tecidos reprodutivos de uma planta
EP1728859A4 (en) SEQUENCE CAPABLE OF ACCELERATING GENE EXPRESSION AT A MODERATELY LOW TEMPERATURE
Liu et al. A single base substitution in the variable pocket of yeast tRNAArg eliminates species-specific aminoacylation

Legal Events

Date Code Title Description
B06A Notification to applicant to reply to the report for non-patentability or inadequacy of the application [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 15/01/2008, OBSERVADAS AS CONDICOES LEGAIS.

B16C Correction of notification of the grant