BRPI0716427A2 - Recombinação homóloga mediada por nuclease de apêndice de zinco - Google Patents

Recombinação homóloga mediada por nuclease de apêndice de zinco

Info

Publication number
BRPI0716427A2
BRPI0716427A2 BRPI0716427-0A2A BRPI0716427A BRPI0716427A2 BR PI0716427 A2 BRPI0716427 A2 BR PI0716427A2 BR PI0716427 A BRPI0716427 A BR PI0716427A BR PI0716427 A2 BRPI0716427 A2 BR PI0716427A2
Authority
BR
Brazil
Prior art keywords
nuclease
appendix
zinc
recombination mediated
homological recombination
Prior art date
Application number
BRPI0716427-0A2A
Other languages
English (en)
Portuguese (pt)
Inventor
Qihua Cai
Jeffrey Miller
William Michael Ainley
Robbi Janette Garrison
Joseph F Petolino
Beth Cori Rubin-Wilson
Lisa Lynn Schulenberg
Andrew Frederick Worden
Original Assignee
Dow Agrosciences Llc
Sangamo Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc, Sangamo Biosciences Inc filed Critical Dow Agrosciences Llc
Publication of BRPI0716427A2 publication Critical patent/BRPI0716427A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/14—Plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
BRPI0716427-0A2A 2006-08-11 2007-08-09 Recombinação homóloga mediada por nuclease de apêndice de zinco BRPI0716427A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US83714706P 2006-08-11 2006-08-11
PCT/US2007/017748 WO2008021207A2 (en) 2006-08-11 2007-08-09 Zinc finger nuclease-mediated homologous recombination

Publications (1)

Publication Number Publication Date
BRPI0716427A2 true BRPI0716427A2 (pt) 2014-03-11

Family

ID=38624400

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0716427-0A2A BRPI0716427A2 (pt) 2006-08-11 2007-08-09 Recombinação homóloga mediada por nuclease de apêndice de zinco

Country Status (19)

Country Link
US (2) US9428756B2 (cg-RX-API-DMAC7.html)
EP (2) EP2049663B1 (cg-RX-API-DMAC7.html)
JP (4) JP2010500029A (cg-RX-API-DMAC7.html)
KR (1) KR101439568B1 (cg-RX-API-DMAC7.html)
CN (2) CN105296527B (cg-RX-API-DMAC7.html)
AR (3) AR062336A1 (cg-RX-API-DMAC7.html)
AU (1) AU2007284748B2 (cg-RX-API-DMAC7.html)
BR (1) BRPI0716427A2 (cg-RX-API-DMAC7.html)
CA (1) CA2660485C (cg-RX-API-DMAC7.html)
DK (1) DK2049663T3 (cg-RX-API-DMAC7.html)
ES (1) ES2532002T3 (cg-RX-API-DMAC7.html)
HK (1) HK1221251A1 (cg-RX-API-DMAC7.html)
IL (1) IL196942A (cg-RX-API-DMAC7.html)
PL (1) PL2049663T3 (cg-RX-API-DMAC7.html)
SI (1) SI2049663T1 (cg-RX-API-DMAC7.html)
TW (1) TWI424061B (cg-RX-API-DMAC7.html)
UA (1) UA106193C2 (cg-RX-API-DMAC7.html)
WO (1) WO2008021207A2 (cg-RX-API-DMAC7.html)
ZA (1) ZA200900787B (cg-RX-API-DMAC7.html)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4968498B2 (ja) 2002-01-23 2012-07-04 ユニバーシティ オブ ユタ リサーチ ファウンデーション ジンクフィンガーヌクレアーゼを用いる、標的化された染色体変異誘発
US7528643B2 (en) * 2003-02-12 2009-05-05 Semiconductor Energy Laboratory Co., Ltd. Semiconductor device, electronic device having the same, and driving method of the same
EP2412812B1 (en) * 2006-12-14 2015-02-11 Dow AgroSciences LLC Optimized non-canonical zinc finger proteins
EP2568048A1 (en) 2007-06-29 2013-03-13 Pioneer Hi-Bred International, Inc. Methods for altering the genome of a monocot plant cell
US9506120B2 (en) * 2007-09-27 2016-11-29 Sangamo Biosciences, Inc. Rapid in vivo identification of biologically active nucleases
DK2247736T3 (da) 2008-02-29 2013-08-05 Monsanto Technology Llc Majsplanteevent mon87460 og sammensætninger og fremgangsmåder til detektion deraf
WO2009114321A2 (en) 2008-03-11 2009-09-17 Precision Biosciencs, Inc. Rationally-designed meganucleases for maize genome engineering
EP2281050B1 (en) * 2008-04-14 2014-04-02 Sangamo BioSciences, Inc. Linear donor constructs for targeted integration
CA2734235C (en) * 2008-08-22 2019-03-26 Sangamo Biosciences, Inc. Methods and compositions for targeted single-stranded cleavage and targeted integration
RU2556376C2 (ru) * 2009-04-07 2015-07-10 ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи Опосредованная наночастицами доставка сиквенс-специфичных нуклеаз
NZ598599A (en) * 2009-08-19 2014-06-27 Dow Agrosciences Llc Aad-1 event das-40278-9, related transgenic corn lines, and event-specific identification thereof
US8598413B2 (en) 2009-08-19 2013-12-03 Dow AgroSciecnes, LLC. AAD-1 event DAS-40278-9, related transgenic corn lines, event-specific identification thereof, and methods of weed control involving AAD-1
CN102639695B (zh) * 2009-10-26 2015-01-21 独立行政法人农业生物资源研究所 转基因植物细胞的制造方法
EP2319872A1 (en) 2009-11-04 2011-05-11 BASF Plant Science GmbH Amylopectin type starch with enhanced retrogradation stability
RU2603252C2 (ru) 2009-11-24 2016-11-27 ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи Событие 416 aad-12, родственные линии трансгенной сои и их событиеспецифичная идентификация
MX336846B (es) * 2010-01-22 2016-02-03 Sangamo Biosciences Inc Alteracion genomica dirigida.
WO2011117249A1 (en) * 2010-03-22 2011-09-29 Philip Morris Products S.A. Modifying enzyme activity in plants
WO2011139336A1 (en) * 2010-04-26 2011-11-10 Sangamo Biosciences, Inc. Genome editing of a rosa locus using nucleases
ES2562421T3 (es) 2010-10-12 2016-03-04 The Children's Hospital Of Philadelphia Métodos y composiciones para tratar la hemofilia B
JP6111200B2 (ja) 2010-12-03 2017-04-05 ダウ アグロサイエンシィズ エルエルシー 除草剤耐性イベント８２６４．４４．０６．１のスタック、関連するトランスジェニックダイズ系統、およびその検出
CA2820706C (en) 2010-12-03 2018-05-22 Ms Technologies, Llc Optimized expression of glyphosate resistance encoding nucleic acid molecules in plant cells
CN102174649B (zh) * 2011-01-18 2013-06-12 陕西师范大学 快速检测锌指核酸酶介导基因定点整合的方法
SG194089A1 (en) 2011-04-27 2013-11-29 Amyris Inc Methods for genomic modification
WO2012151254A1 (en) 2011-05-02 2012-11-08 Board Of Regents Of The University Of Nebraska Plants with useful traits and related methods
WO2012159079A1 (en) 2011-05-18 2012-11-22 The Parkinson's Institute Assay to determine lrrk2 activity in parkinson's disease
EP2535416A1 (en) 2011-05-24 2012-12-19 BASF Plant Science Company GmbH Development of phytophthora resistant potato with increased yield
BR102012019434B1 (pt) 2011-07-26 2021-11-09 Dow Agrosciences Llc Métodos de controle de pestes, de insetos, molécula e sequência de dna diagnóstica para o evento de soja 9582.814.19.1
IL293650B2 (en) 2011-09-21 2024-10-01 Sangamo Therapeutics Inc Methods and compositions for regulation of transgene expression
EP2612918A1 (en) 2012-01-06 2013-07-10 BASF Plant Science Company GmbH In planta recombination
MX349180B (es) 2012-01-23 2017-07-17 Dow Agrosciences Llc Algodón tolerante a herbicidas evento pdab4468.19-10.3.
AR089873A1 (es) 2012-02-01 2014-09-24 Dow Agrosciences Llc Vegetales resistentes al glifosato y metodos asociados
EP2861737B1 (en) 2012-06-19 2019-04-17 Regents Of The University Of Minnesota Gene targeting in plants using dna viruses
UA118090C2 (uk) 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив
UA119135C2 (uk) 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
US20140075593A1 (en) * 2012-09-07 2014-03-13 Dow Agrosciences Llc Fluorescence activated cell sorting (facs) enrichment to generate plants
CN105264067B (zh) 2012-09-07 2020-11-10 美国陶氏益农公司 Fad3性能基因座及相应的能够诱导靶向断裂的靶位点特异性结合蛋白
EP3679785A3 (en) 2013-04-05 2020-09-16 Dow AgroSciences LLC Methods and compositions for integration of an exogenous sequence within the genome of plants
EP3008186B1 (en) 2013-06-14 2018-11-28 Cellectis Methods for non-transgenic genome editing in plants
US10767188B2 (en) 2013-09-25 2020-09-08 Nutech Ventures Methods and compositions for obtaining useful plant traits
CA2928855A1 (en) 2013-11-04 2015-05-07 Dow Agrosciences Llc Optimal maize loci
WO2015066637A1 (en) * 2013-11-04 2015-05-07 Dow Agrosciences Llc A universal donor system for gene targeting
EP3862434A1 (en) 2013-11-04 2021-08-11 Dow AgroSciences LLC Optimal soybean loci
UY35812A (es) 2013-11-04 2015-05-29 Dow Agrosciences Llc ?loci de maíz óptimos?.
MX2016007797A (es) 2013-12-19 2016-09-07 Amyris Inc Metodos para integracion genomica.
US10683513B2 (en) 2013-12-31 2020-06-16 Dow Agrosciences Llc Tissue-specific expression and hybrid plant production
FI3169778T3 (fi) 2014-07-14 2023-12-13 Univ Washington State Ituradan soluja ablatoiva nanos-geeninpoisto
WO2016098078A2 (en) 2014-12-19 2016-06-23 Novartis Ag Dimerization switches and uses thereof
US20160281055A1 (en) 2015-03-20 2016-09-29 Dow Agrosciences Llc Incorporation of phosphatidylcholine in a media composition
MX382223B (es) * 2015-05-12 2025-03-13 Sangamo Therapeutics Inc Regulacion de expresion genica mediada por nucleasa.
CN109475107A (zh) 2015-08-06 2019-03-15 密苏里大学管理者 具有修饰的cd163基因的抗病原体动物
RU2021114137A (ru) 2016-01-27 2021-05-25 Онкорус, Инк. Онколитические вирусные векторы и их применение
WO2017143071A1 (en) 2016-02-18 2017-08-24 The Regents Of The University Of California Methods and compositions for gene editing in stem cells
EP3429567B1 (en) 2016-03-16 2024-01-10 The J. David Gladstone Institutes Methods and compositions for treating obesity and/or diabetes and for identifying candidate treatment agents
US11293033B2 (en) 2016-05-18 2022-04-05 Amyris, Inc. Compositions and methods for genomic integration of nucleic acids into exogenous landing pads
US11078481B1 (en) 2016-08-03 2021-08-03 KSQ Therapeutics, Inc. Methods for screening for cancer targets
BR112019003327A2 (pt) * 2016-08-24 2019-07-02 Sangamo Therapeutics, Inc. nucleases manipuladas de alvo específico
CA3034882A1 (en) 2016-08-24 2018-03-01 Sangamo Therapeutics, Inc. Regulation of gene expression using engineered nucleases
US11078483B1 (en) 2016-09-02 2021-08-03 KSQ Therapeutics, Inc. Methods for measuring and improving CRISPR reagent function
KR20240172763A (ko) 2016-12-14 2024-12-10 리간달 인코포레이티드 핵산 및/또는 단백질 적재물 전달을 위한 조성물 및 방법
MX2019015188A (es) 2017-06-15 2020-08-03 Univ California Inserciones de adn no virales orientadas.
US11612625B2 (en) 2017-07-26 2023-03-28 Oncorus, Inc. Oncolytic viral vectors and uses thereof
MX2020004325A (es) 2017-10-27 2020-11-09 Univ California Reemplazo dirigido de receptores de células t endógenos.
EP3728587A4 (en) 2017-12-18 2022-01-26 Syngenta Participations Ag SITES FOR TARGETED INSERTION IN THE MAIZE GENOME
KR20210069692A (ko) * 2018-10-02 2021-06-11 상가모 테라퓨틱스, 인코포레이티드 조작된 유전자 조정제
WO2020072677A1 (en) 2018-10-02 2020-04-09 Sangamo Therapeutics, Inc. Methods and compositions for modulation of tau proteins
US20200157531A1 (en) * 2018-11-16 2020-05-21 Wisconsin Alumni Research Foundation Method to enhance screening for homologous recombination in genome edited cells using recombination-activated fluorescent donor delivery vector
WO2020163856A1 (en) 2019-02-10 2020-08-13 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Modified mitochondrion and methods of use thereof
WO2021011936A2 (en) 2019-07-18 2021-01-21 University Of Rochester Cell-type selective immunoprotection of cells
WO2021028359A1 (en) 2019-08-09 2021-02-18 Sangamo Therapeutics France Controlled expression of chimeric antigen receptors in t cells
CN111462823B (zh) * 2020-04-08 2022-07-12 西安交通大学 一种基于dna测序数据的同源重组缺陷判定方法
MX2023005665A (es) 2020-11-16 2023-08-01 Pig Improvement Co Uk Ltd Animales resistentes a la influenza a que tienen genes de anp32 editados.
WO2022173531A1 (en) 2021-02-10 2022-08-18 Oncorus, Inc. Compounds, compositions, and methods of using thereof
WO2023064928A2 (en) 2021-10-14 2023-04-20 Arsenal Biosciences, Inc. Immune cells having co-expressed shrnas and logic gate systems
WO2023081756A1 (en) 2021-11-03 2023-05-11 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Precise genome editing using retrons
US20250064032A1 (en) 2021-12-10 2025-02-27 Pig Improvement Company Uk Limited Editing tmprss2/4 for disease resistance in livestock
WO2023141602A2 (en) 2022-01-21 2023-07-27 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
WO2024013514A2 (en) 2022-07-15 2024-01-18 Pig Improvement Company Uk Limited Gene edited livestock animals having coronavirus resistance
WO2024044723A1 (en) 2022-08-25 2024-02-29 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
AU2024246543A1 (en) 2023-03-31 2025-10-30 Briacell Therapeutics Corp. Methods for enhancing the immunogenicity of cellular vaccines
WO2025049959A2 (en) 2023-09-01 2025-03-06 Renagade Therapeutics Management Inc. Gene editing systems, compositions, and methods for treatment of vexas syndrome
WO2025174765A1 (en) 2024-02-12 2025-08-21 Renagade Therapeutics Management Inc. Lipid nanoparticles comprising coding rna molecules for use in gene editing and as vaccines and therapeutic agents

Family Cites Families (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0222493A1 (en) 1985-10-04 1987-05-20 Lubrizol Genetics Inc. TR-based sub-TI plasmids
US5422251A (en) 1986-11-26 1995-06-06 Princeton University Triple-stranded nucleic acids
EP0342926B1 (en) 1988-05-17 1994-09-28 Mycogen Plant Science, Inc. Plant ubiquitin promoter system
US5176996A (en) 1988-12-20 1993-01-05 Baylor College Of Medicine Method for making synthetic oligonucleotides which bind specifically to target sites on duplex DNA molecules, by forming a colinear triplex, the synthetic oligonucleotides and methods of use
FR2646438B1 (fr) 1989-03-20 2007-11-02 Pasteur Institut Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration
US5420032A (en) 1991-12-23 1995-05-30 Universitge Laval Homing endonuclease which originates from chlamydomonas eugametos and recognizes and cleaves a 15, 17 or 19 degenerate double stranded nucleotide sequence
US5436150A (en) 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
US5487994A (en) 1992-04-03 1996-01-30 The Johns Hopkins University Insertion and deletion mutants of FokI restriction endonuclease
US5356802A (en) 1992-04-03 1994-10-18 The Johns Hopkins University Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease
US5792632A (en) 1992-05-05 1998-08-11 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
EP1983056A1 (en) 1992-07-07 2008-10-22 Japan Tobacco Inc. Method for transforming monocotyledons
US6140466A (en) 1994-01-18 2000-10-31 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
US6242568B1 (en) 1994-01-18 2001-06-05 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
AU704601B2 (en) 1994-01-18 1999-04-29 Scripps Research Institute, The Zinc finger protein derivatives and methods therefor
US5585245A (en) 1994-04-22 1996-12-17 California Institute Of Technology Ubiquitin-based split protein sensor
USRE39229E1 (en) 1994-08-20 2006-08-08 Gendaq Limited Binding proteins for recognition of DNA
GB9824544D0 (en) 1998-11-09 1999-01-06 Medical Res Council Screening system
US5789538A (en) 1995-02-03 1998-08-04 Massachusetts Institute Of Technology Zinc finger proteins with high affinity new DNA binding specificities
US5773695A (en) 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
US5925523A (en) 1996-08-23 1999-07-20 President & Fellows Of Harvard College Intraction trap assay, reagents and uses thereof
GB2338237B (en) 1997-02-18 2001-02-28 Actinova Ltd In vitro peptide or protein expression library
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
US6342345B1 (en) 1997-04-02 2002-01-29 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by reporter subunit complementation
GB9710807D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
GB9710809D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
AU755063B2 (en) 1997-06-12 2002-12-05 Dow Agrosciences Llc Regulatory sequences for transgenic plants
AU760113C (en) * 1997-11-18 2004-04-22 Pioneer Hi-Bred International, Inc. Compositions and methods for genetic modification of plants
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
AR014491A1 (es) 1998-01-29 2001-02-28 Dow Agrosciences Llc Metodo para obtener plantas transgenicas fertiles de gossypium hirsutum.
US6410248B1 (en) 1998-01-30 2002-06-25 Massachusetts Institute Of Technology General strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites
US6479626B1 (en) 1998-03-02 2002-11-12 Massachusetts Institute Of Technology Poly zinc finger proteins with improved linkers
US6140081A (en) 1998-10-16 2000-10-31 The Scripps Research Institute Zinc finger binding domains for GNN
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6453242B1 (en) 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
US7030215B2 (en) 1999-03-24 2006-04-18 Sangamo Biosciences, Inc. Position dependent recognition of GNN nucleotide triplets by zinc fingers
US6794136B1 (en) 2000-11-20 2004-09-21 Sangamo Biosciences, Inc. Iterative optimization in the design of binding proteins
EP1236045B1 (en) 1999-12-06 2005-11-09 Sangamo Biosciences Inc. Methods of using randomized libraries of zinc finger proteins for the identification of gene function
AU2001226935B2 (en) 2000-01-24 2006-06-22 Gendaq Limited Nucleic acid binding polypeptides characterized by flexible linkers connected nucleic acid binding modules
US20020061512A1 (en) 2000-02-18 2002-05-23 Kim Jin-Soo Zinc finger domains and methods of identifying same
AU2001253914B2 (en) 2000-04-28 2006-06-08 Sangamo Therapeutics, Inc. Targeted modification of chromatin structure
WO2001088197A2 (en) 2000-05-16 2001-11-22 Massachusetts Institute Of Technology Methods and compositions for interaction trap assays
JP2002060786A (ja) 2000-08-23 2002-02-26 Kao Corp 硬質表面用殺菌防汚剤
EP1353941B1 (en) 2001-01-22 2013-03-13 Sangamo BioSciences, Inc. Modified zinc finger binding proteins
GB0108491D0 (en) 2001-04-04 2001-05-23 Gendaq Ltd Engineering zinc fingers
EP1377248A2 (en) 2001-04-05 2004-01-07 The Regents of the University of California Robotic device for locomotor training
WO2003016496A2 (en) 2001-08-20 2003-02-27 The Scripps Research Institute Zinc finger binding domains for cnn
AU2002360986A1 (en) 2001-12-20 2003-07-09 Sungene Gmbh And Co. Kgaa Methods for the transformation of vegetal plastids
US7262054B2 (en) 2002-01-22 2007-08-28 Sangamo Biosciences, Inc. Zinc finger proteins for DNA binding and gene regulation in plants
JP4968498B2 (ja) 2002-01-23 2012-07-04 ユニバーシティ オブ ユタ リサーチ ファウンデーション ジンクフィンガーヌクレアーゼを用いる、標的化された染色体変異誘発
EP2368982A3 (en) 2002-03-21 2011-10-12 Sangamo BioSciences, Inc. Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
US9447434B2 (en) 2002-09-05 2016-09-20 California Institute Of Technology Use of chimeric nucleases to stimulate gene targeting
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
CA2534296C (en) 2003-08-08 2013-03-26 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US20050102713A1 (en) 2003-11-07 2005-05-12 Merlo Donald J. Use of untranslated region of osmotin gene to enhance transgene expression in plants
AU2005224324B2 (en) 2004-03-17 2009-12-24 Basf Plant Science Gmbh Improved constructs for marker excision based on dual-function selection marker
AU2005287547B2 (en) 2004-09-23 2010-09-02 Basf Plant Science Gmbh Recombination cassettes and methods for sequence excision in plants
SI1869186T1 (sl) 2005-04-04 2011-02-28 Bayer Bioscience Nv Postopki in sredstva za odstranitev izbranega DNA zaporedja
SG10201508995QA (en) 2005-07-26 2015-11-27 Sangamo Biosciences Inc Targeted integration and expression of exogenous nucleic acid sequences

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