BRPI0620552A2 - polinucleotìdeo isolado, polipeptìdeo delta-9 elongase, construção recombinante, célula vegetal, método para transformar uma célula, método para produção de uma planta transgênica, sementes transgênicas, método para fabricar ácidos graxos poliinsaturados de cadeia longa, óleos, método para produzir pelo menos um ácido graxo poliinsaturado, plantas de semente oleaginosa, sementes, alimentos, fragmento de ácido nucléico isolado e progênies de plantas - Google Patents

polinucleotìdeo isolado, polipeptìdeo delta-9 elongase, construção recombinante, célula vegetal, método para transformar uma célula, método para produção de uma planta transgênica, sementes transgênicas, método para fabricar ácidos graxos poliinsaturados de cadeia longa, óleos, método para produzir pelo menos um ácido graxo poliinsaturado, plantas de semente oleaginosa, sementes, alimentos, fragmento de ácido nucléico isolado e progênies de plantas Download PDF

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Publication number
BRPI0620552A2
BRPI0620552A2 BRPI0620552-6A BRPI0620552A BRPI0620552A2 BR PI0620552 A2 BRPI0620552 A2 BR PI0620552A2 BR PI0620552 A BRPI0620552 A BR PI0620552A BR PI0620552 A2 BRPI0620552 A2 BR PI0620552A2
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Brazil
Prior art keywords
delta
seq
elongase
desaturase
plant
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Application number
BRPI0620552-6A
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English (en)
Portuguese (pt)
Inventor
Howard Glenn Damude
Brian Macgonigle
Quinn Qun Zhu
Zhixiong Xue
Original Assignee
Du Pont
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Publication of BRPI0620552A2 publication Critical patent/BRPI0620552A2/pt

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11BPRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00Production of fats or fatty oils from raw materials
    • C11B1/02Pretreatment
    • C11B1/025Pretreatment by enzymes or microorganisms, living or dead
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1025Acyltransferases (2.3)
    • C12N9/1029Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409Fatty acids
    • C12P7/6427Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436Fatty acid esters
    • C12P7/6445Glycerides
    • C12P7/6458Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436Fatty acid esters
    • C12P7/6445Glycerides
    • C12P7/6463Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436Fatty acid esters
    • C12P7/6445Glycerides
    • C12P7/6472Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Feed For Specific Animals (AREA)
  • Fodder In General (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
BRPI0620552-6A 2005-11-23 2006-11-16 polinucleotìdeo isolado, polipeptìdeo delta-9 elongase, construção recombinante, célula vegetal, método para transformar uma célula, método para produção de uma planta transgênica, sementes transgênicas, método para fabricar ácidos graxos poliinsaturados de cadeia longa, óleos, método para produzir pelo menos um ácido graxo poliinsaturado, plantas de semente oleaginosa, sementes, alimentos, fragmento de ácido nucléico isolado e progênies de plantas BRPI0620552A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US73998905P 2005-11-23 2005-11-23
US60/739,989 2005-11-23
PCT/US2006/044676 WO2007061845A2 (en) 2005-11-23 2006-11-16 Delta-9 elongases and their use in making polyunsaturated fatty acids

Publications (1)

Publication Number Publication Date
BRPI0620552A2 true BRPI0620552A2 (pt) 2011-11-22

Family

ID=37836626

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0620552-6A BRPI0620552A2 (pt) 2005-11-23 2006-11-16 polinucleotìdeo isolado, polipeptìdeo delta-9 elongase, construção recombinante, célula vegetal, método para transformar uma célula, método para produção de uma planta transgênica, sementes transgênicas, método para fabricar ácidos graxos poliinsaturados de cadeia longa, óleos, método para produzir pelo menos um ácido graxo poliinsaturado, plantas de semente oleaginosa, sementes, alimentos, fragmento de ácido nucléico isolado e progênies de plantas

Country Status (11)

Country Link
US (6) US20070118929A1 (enExample)
EP (2) EP1957641A2 (enExample)
JP (1) JP5123861B2 (enExample)
KR (1) KR20080071190A (enExample)
CN (1) CN101365788B (enExample)
AU (2) AU2006318738B2 (enExample)
BR (1) BRPI0620552A2 (enExample)
CA (2) CA2625855C (enExample)
DK (1) DK1951866T3 (enExample)
NO (1) NO20082466L (enExample)
WO (2) WO2007061742A1 (enExample)

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BR112012015641A2 (pt) * 2009-12-24 2015-11-24 Du Pont "polinucleotídeo isolado, construtor de dna recombinante, célula hospedeira, método para produzir uma planta transformada, para aumentar o teor de pelo menos um um ácido graxo, para aumentar pelo menos uma eficiencia de conversão, semente transgênica, plantas, óleos, alimento humano ou de animal e plantas de progênie"
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CN103282474A (zh) 2010-04-22 2013-09-04 纳幕尔杜邦公司 从微生物生物质中获得包含多不饱和脂肪酸的组合物的方法
BR112012033668A2 (pt) 2010-07-01 2017-06-13 Du Pont planta e semente transgênica, método para a produção de um planta transgênica, produto e/ou subproduto obtido a partir da semente transgênica, polinucleotídeo isolado e planta ou semente compreendendo uma cosntrução de dna recombinante
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US8703473B2 (en) * 2010-08-26 2014-04-22 E I Du Pont De Nemours And Company Recombinant microbial host cells for high eicosapentaenoic acid production
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BR112013016769A2 (pt) 2010-12-30 2016-10-11 Du Pont yarrowia lipolytica transformada e método para produzir pelo menos um produto não nativo de interesse
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US7645604B2 (en) 2010-01-12
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CN101365788B (zh) 2012-11-14
US8048653B2 (en) 2011-11-01
CN101365788A (zh) 2009-02-11
JP5123861B2 (ja) 2013-01-23
CA2625855A1 (en) 2007-05-31
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