FR3053052B1 - Microalgue modifiee pour une production enrichie en tag - Google Patents

Microalgue modifiee pour une production enrichie en tag Download PDF

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Publication number
FR3053052B1
FR3053052B1 FR1656007A FR1656007A FR3053052B1 FR 3053052 B1 FR3053052 B1 FR 3053052B1 FR 1656007 A FR1656007 A FR 1656007A FR 1656007 A FR1656007 A FR 1656007A FR 3053052 B1 FR3053052 B1 FR 3053052B1
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FR
France
Prior art keywords
production
tag
modified microalgae
enriched production
relates
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Active
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FR1656007A
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English (en)
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FR3053052A1 (fr
Inventor
Lina Juana Dolch
Camille Rak
Fabrice Rebeille
Juliette Jouhet
Marina Leterrier
Eric Marechal
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fermentalg SA
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Original Assignee
Commissariat a lEnergie Atomique CEA
Fermentalg SA
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
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Publication date
Application filed by Commissariat a lEnergie Atomique CEA, Fermentalg SA, Commissariat a lEnergie Atomique et aux Energies Alternatives CEA filed Critical Commissariat a lEnergie Atomique CEA
Priority to FR1656007A priority Critical patent/FR3053052B1/fr
Priority to US16/308,226 priority patent/US10724011B2/en
Priority to PCT/EP2017/065869 priority patent/WO2018002059A1/fr
Priority to CN201780040195.4A priority patent/CN109477060B/zh
Priority to AU2017288438A priority patent/AU2017288438B2/en
Publication of FR3053052A1 publication Critical patent/FR3053052A1/fr
Priority to IL263792A priority patent/IL263792B2/en
Application granted granted Critical
Publication of FR3053052B1 publication Critical patent/FR3053052B1/fr
Active legal-status Critical Current
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01199—Very-long-chain 3-oxoacyl-CoA synthase (2.3.1.199)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

La présente invention concerne une microalgue génétiquement modifiée par inhibition de l'activité de l'enzyme élongase-Δ0 (ou Δ0-ELO), ayant pour substrat l'acide palmitique, un acide gras saturé, et associée à la production de MGDG (Mono-Galactosyl-Diacyl-Glycerol). Elle concerne également un procédé de culture de ladite microalgue pour une production enrichie en TAG (Tri-Acyl-Glycerols ) et la récupération desdits TAG.
FR1656007A 2016-06-28 2016-06-28 Microalgue modifiee pour une production enrichie en tag Active FR3053052B1 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
FR1656007A FR3053052B1 (fr) 2016-06-28 2016-06-28 Microalgue modifiee pour une production enrichie en tag
US16/308,226 US10724011B2 (en) 2016-06-28 2017-06-27 Alga modified for increased tag production
PCT/EP2017/065869 WO2018002059A1 (fr) 2016-06-28 2017-06-27 Microalgue modifiée pour une production enrichie en tag
CN201780040195.4A CN109477060B (zh) 2016-06-28 2017-06-27 增加tag产量的修饰藻类
AU2017288438A AU2017288438B2 (en) 2016-06-28 2017-06-27 Alga modified for increased tag production
IL263792A IL263792B2 (en) 2016-06-28 2018-12-18 Algae modified for increased production of triacylglycerols

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR1656007A FR3053052B1 (fr) 2016-06-28 2016-06-28 Microalgue modifiee pour une production enrichie en tag

Publications (2)

Publication Number Publication Date
FR3053052A1 FR3053052A1 (fr) 2017-12-29
FR3053052B1 true FR3053052B1 (fr) 2021-02-12

Family

ID=57233580

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1656007A Active FR3053052B1 (fr) 2016-06-28 2016-06-28 Microalgue modifiee pour une production enrichie en tag

Country Status (6)

Country Link
US (1) US10724011B2 (fr)
CN (1) CN109477060B (fr)
AU (1) AU2017288438B2 (fr)
FR (1) FR3053052B1 (fr)
IL (1) IL263792B2 (fr)
WO (1) WO2018002059A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3053052B1 (fr) * 2016-06-28 2021-02-12 Fermentalg Microalgue modifiee pour une production enrichie en tag

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5614393A (en) 1991-10-10 1997-03-25 Rhone-Poulenc Agrochimie Production of γ-linolenic acid by a Δ6-desaturase
AU731785B2 (en) 1996-03-28 2001-04-05 Gist-Brocades B.V. Preparation of microbial polyunsaturated fatty acid containing oil from pasteurised biomass
GB0107510D0 (en) * 2001-03-26 2001-05-16 Univ Bristol New elongase gene and a process for the production of -9-polyunsaturated fatty acids
JP5123861B2 (ja) * 2005-11-23 2013-01-23 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー Δ９エロンガーゼおよびそれらの多価不飽和脂肪酸製造における使用
US7678560B2 (en) * 2006-05-17 2010-03-16 E.I. Du Pont De Nemours And Company Δ 5 desaturase and its use in making polyunsaturated fatty acids
AU2009240794A1 (en) * 2008-04-25 2009-10-29 Commonwealth Scientific Industrial Research Organisation Polypeptides and methods for producing triacylglycerols comprising modified fatty acids
US8809046B2 (en) * 2011-04-28 2014-08-19 Aurora Algae, Inc. Algal elongases
FR2964667B1 (fr) 2010-09-15 2014-08-22 Fermentalg Procede de culture d'algues unicellulaires mixotrophes en presence d'un apport lumineux discontinu sous forme de flashs
CN102226196B (zh) * 2011-05-18 2012-11-21 上海海洋大学 一种编码缺刻缘绿藻δ-6脂肪酸延长酶的dna序列及其应用
EP3013939A1 (fr) 2013-06-25 2016-05-04 Cellectis Diatomées modifiées pour production de biocarburant
FR3008422B1 (fr) 2013-07-12 2017-11-17 Fermentalg Procede de culture cellulaire decouple
FR3053052B1 (fr) * 2016-06-28 2021-02-12 Fermentalg Microalgue modifiee pour une production enrichie en tag

Also Published As

Publication number Publication date
US20190264183A1 (en) 2019-08-29
AU2017288438B2 (en) 2022-09-15
CN109477060A (zh) 2019-03-15
IL263792B2 (en) 2023-07-01
IL263792A (en) 2019-02-03
WO2018002059A1 (fr) 2018-01-04
AU2017288438A1 (en) 2019-01-03
IL263792B1 (en) 2023-03-01
CN109477060B (zh) 2022-06-17
FR3053052A1 (fr) 2017-12-29
US10724011B2 (en) 2020-07-28
WO2018002059A9 (fr) 2018-04-05

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