BRPI0612288A2 - amilase modificada de pseudomonas saccharophilia - Google Patents

amilase modificada de pseudomonas saccharophilia

Info

Publication number
BRPI0612288A2
BRPI0612288A2 BRPI0612288-4A BRPI0612288A BRPI0612288A2 BR PI0612288 A2 BRPI0612288 A2 BR PI0612288A2 BR PI0612288 A BRPI0612288 A BR PI0612288A BR PI0612288 A2 BRPI0612288 A2 BR PI0612288A2
Authority
BR
Brazil
Prior art keywords
polypeptide
amino acid
acid mutation
amylase
pseudomonas saccharophilia
Prior art date
Application number
BRPI0612288-4A
Other languages
English (en)
Inventor
Casper Tune Berg
Patrick Maria Franciscus Derkx
Carol Fioresi
Gijsbert Gerritse
Kellet-Smith Anja Hemmingen
Karsten Kragh
Wei Liu
Andrew Shaw
Bo Spange Sorensen
Thoudahl Charlotte Refdahl
Original Assignee
Danisco
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco, Genencor Int filed Critical Danisco
Publication of BRPI0612288A2 publication Critical patent/BRPI0612288A2/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D8/00—Methods for preparing or baking dough
    • A21D8/02—Methods for preparing dough; Treating dough prior to baking
    • A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2425—Beta-amylase (3.2.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Polymers & Plastics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)
  • Cereal-Derived Products (AREA)

Abstract

AMILASE MODIFICADA DE PSEUDONONAS SACCHIAROPHILIA A presente invenção descreve um polipeptídeo variante PS4 derivável de um polipeptídeo de origem com atividade amílase selecionado do grupo consistindo de: (a) um polipeptídeo compreendendo uma mutação de aminoácido em cada uma das posições 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 e 334; (b) um polipeptídeo compreendendo uma mutação de aminoácido em cada uma das posições 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 e 334; (o) um polipeptídeo compreendendo uma mutação de aminoácido em cada uma das posições 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 e 334; e (d) um polipeptídeo compreendendo uma mutação de aminoácido em cada uma das posições 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 e 334; com referência à numeração de posição de uma seqúência exoamilase de Pseudomonas saccharophilia mostrada como SEQ ID NO: 1, usos de tal polipeptídeo como um aditivo de ração ou alimentício e ácidos nuclélcos que o codificam.
BRPI0612288-4A 2005-07-07 2006-07-07 amilase modificada de pseudomonas saccharophilia BRPI0612288A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US69730205P 2005-07-07 2005-07-07
PCT/GB2006/002513 WO2007007053A1 (en) 2005-07-07 2006-07-07 Modified amylase from pseudomonas saccharophilia

Publications (1)

Publication Number Publication Date
BRPI0612288A2 true BRPI0612288A2 (pt) 2009-01-27

Family

ID=37331082

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0612288-4A BRPI0612288A2 (pt) 2005-07-07 2006-07-07 amilase modificada de pseudomonas saccharophilia

Country Status (11)

Country Link
US (2) US20070141693A1 (pt)
EP (1) EP1907538A1 (pt)
JP (1) JP2008544751A (pt)
KR (1) KR20080023746A (pt)
CN (1) CN101238210A (pt)
AU (1) AU2006268418A1 (pt)
BR (1) BRPI0612288A2 (pt)
CA (1) CA2614274A1 (pt)
MX (1) MX2008000374A (pt)
RU (1) RU2008104637A (pt)
WO (1) WO2007007053A1 (pt)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1654355T3 (da) * 2003-06-13 2010-08-09 Danisco Pseudomonas polypeptidvarianter med ikke-maltogen exoamylaseaktivitet og deres anvendelse til fremstilling af fødevarer
CN102796717A (zh) * 2003-07-07 2012-11-28 金克克国际有限公司 外切特异性的淀粉酶多肽、编码那些多肽的核酸及其应用
US8143048B2 (en) * 2003-07-07 2012-03-27 Danisco A/S Exo-specific amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof
DK2295557T3 (en) * 2004-07-07 2017-12-11 Genencor Int EXOAMYLASE VARIANTS
US8030050B2 (en) * 2005-07-07 2011-10-04 Danisco A/S Modified amylases from Pseudomonas species
KR20080023746A (ko) * 2005-07-07 2008-03-14 대니스코 에이/에스 슈도모나스 사카로필리아에 의해 변형된 아밀라아제
ES2644745T3 (es) * 2006-06-19 2017-11-30 Dupont Nutrition Biosciences Aps Polipéptido
US7666637B2 (en) * 2006-09-05 2010-02-23 Xuan Nghinh Nguyen Integrated process for separation of lignocellulosic components to fermentable sugars for production of ethanol and chemicals
EP2238244A1 (en) * 2008-01-02 2010-10-13 Danisco A/S Pseudomonas saccharophila g4-amylase variants and uses thereof
JP2010148488A (ja) * 2008-12-26 2010-07-08 Biomaterial In Tokyo Co Ltd カンジダ・グラブラータ（Ｃ．ｇｌａｂｒａｔａ）を用いるエタノール製造方法
US20120301927A1 (en) * 2009-04-10 2012-11-29 Danisco Us Inc. Production Of Maltotetraose Syrup Using A Pseudomonas Saccharophila Maltotetraohydrolase Variant
EP3591046B1 (en) 2009-05-19 2021-04-21 DuPont Nutrition Biosciences ApS Amylase polypeptide
EP3392268B1 (en) 2010-03-29 2021-06-30 DuPont Nutrition Biosciences ApS Polypeptides having transgalactosylating activity
MX355356B (es) 2011-10-17 2018-04-17 Novozymes As Variantes de alfa-amilasa y polinucleotidos que codifican las mismas.
CN103890170B (zh) 2011-10-17 2018-02-02 诺维信公司 α‑淀粉酶变体以及对其进行编码的多核苷酸
ES2650444T3 (es) 2012-06-08 2018-01-18 Dupont Nutrition Biosciences Aps Polipéptidos que tienen actividad transgalactosilante
US9850512B2 (en) 2013-03-15 2017-12-26 The Research Foundation For The State University Of New York Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield
WO2015086027A1 (en) 2013-12-09 2015-06-18 Carlsberg A/S Stable haze for beverages
EP3082456A1 (en) 2013-12-11 2016-10-26 DuPont Nutrition Biosciences ApS A method for preparing a dairy product having a stable content of galacto-oligosaccharide(s)
US9951363B2 (en) 2014-03-14 2018-04-24 The Research Foundation for the State University of New York College of Environmental Science and Forestry Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects
MX2017005981A (es) 2014-11-07 2017-06-29 Dupont Nutrition Biosci Aps Celula hospedadora recombinante que expresa actividad de beta-galactosidasa y/o transgalactosidante deficiente en mananasa, celulasa y pectinasa.
DK3607097T3 (da) 2017-04-07 2023-09-18 Dupont Nutrition Biosci Aps Bacillus-værtsceller, der danner beta-galactosidaser og lactaser i fravær af p-nitrobenzylesterase-sideaktivitet
EP4071242A1 (en) * 2021-04-06 2022-10-12 DuPont Nutrition Biosciences ApS Amylase polypeptides with improved properties

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DE3260491D1 (en) * 1981-04-13 1984-09-06 Takeda Chemical Industries Ltd Pseudo-aminosugars, their production and use
JP2660836B2 (ja) * 1987-07-08 1997-10-08 株式会社 林原生物化学研究所 マルトテトラオース生成アミラーゼ活性を有するポリペプチドとその用途
DE4017595A1 (de) * 1990-05-31 1991-12-05 Consortium Elektrochem Ind Maltopentaose produzierende amylasen
JP3533239B2 (ja) * 1994-03-01 2004-05-31 株式会社林原生物化学研究所 マルトヘキサオース・マルトヘプタオース生成アミラーゼとその製造方法並びに用途
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US6093562A (en) * 1996-02-05 2000-07-25 Novo Nordisk A/S Amylase variants
ATE490312T1 (de) * 1998-02-27 2010-12-15 Novozymes As Maltogene alpha-amylase varianten
BR9909280A (pt) * 1998-04-01 2000-11-21 Danisco Exoamilases não maltogênicas e sua utilização no retardo de retrogradação de amido
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US8143048B2 (en) * 2003-07-07 2012-03-27 Danisco A/S Exo-specific amylase polypeptides, nucleic acids encoding those polypeptides and uses thereof
CN102796717A (zh) * 2003-07-07 2012-11-28 金克克国际有限公司 外切特异性的淀粉酶多肽、编码那些多肽的核酸及其应用
US20060008890A1 (en) * 2004-07-07 2006-01-12 Kragh Karsten M Polypeptide
US20060008888A1 (en) * 2004-07-07 2006-01-12 Kragh Karsten M Polypeptide
US20060018997A1 (en) * 2004-07-07 2006-01-26 Kragh Karsten M Polypeptide
US20060073583A1 (en) * 2004-09-22 2006-04-06 Kragh Karsten M Polypeptide
US8030050B2 (en) * 2005-07-07 2011-10-04 Danisco A/S Modified amylases from Pseudomonas species
KR20080023746A (ko) * 2005-07-07 2008-03-14 대니스코 에이/에스 슈도모나스 사카로필리아에 의해 변형된 아밀라아제

Also Published As

Publication number Publication date
EP1907538A1 (en) 2008-04-09
JP2008544751A (ja) 2008-12-11
WO2007007053A1 (en) 2007-01-18
KR20080023746A (ko) 2008-03-14
CA2614274A1 (en) 2007-01-18
RU2008104637A (ru) 2009-08-20
CN101238210A (zh) 2008-08-06
AU2006268418A1 (en) 2007-01-18
MX2008000374A (es) 2008-03-07
US20080292747A1 (en) 2008-11-27
US20070141693A1 (en) 2007-06-21

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Legal Events

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 6A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: NAO APRESENTADA A GUIA DE CUMPRIMENTO DE EXIGENCIA. REFERENTE A 6A ANUIDADE.