BR112018077303A2 - planta transgênica com resistência a herbicida - Google Patents

planta transgênica com resistência a herbicida

Info

Publication number
BR112018077303A2
BR112018077303A2 BR112018077303-4A BR112018077303A BR112018077303A2 BR 112018077303 A2 BR112018077303 A2 BR 112018077303A2 BR 112018077303 A BR112018077303 A BR 112018077303A BR 112018077303 A2 BR112018077303 A2 BR 112018077303A2
Authority
BR
Brazil
Prior art keywords
transgenic plant
protein
herbicide
amino acid
acid sequence
Prior art date
Application number
BR112018077303-4A
Other languages
English (en)
Inventor
Tanetani Yoshitaka
Kawai Kiyoshi
Original Assignee
Kumiai Chemical Industry Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kumiai Chemical Industry Co., Ltd. filed Critical Kumiai Chemical Industry Co., Ltd.
Publication of BR112018077303A2 publication Critical patent/BR112018077303A2/pt

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1085Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1088Glutathione transferase (2.5.1.18)
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/20Brassicaceae, e.g. canola, broccoli or rucola
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/80Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y205/00Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01018Glutathione transferase (2.5.1.18)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Developmental Biology & Embryology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Physiology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

a presente invenção refere-se a identificar glutationa-s-transferase que exibe as atividades para metabolizar e desintoxicar um derivado de isoxazolina, tal como piroxassulfona. a invenção proporciona um método para cultivar uma planta transgênica na qual foi introduzido um ácido nucleico que codifica uma proteína (a ou b) abaixo na presença de derivados de isoxazolina: (a) uma proteína compreendendo a sequência de aminoácidos como mostrada na seq id no: 2; ou (b) uma proteína compreendendo uma sequência de aminoácidos tendo 80% ou mais de identidade com a sequência de amino-ácidos, como mostrada na seq id no: 2 e tendo a atividade da glutationa-s-transferase.
BR112018077303-4A 2016-07-04 2017-07-04 planta transgênica com resistência a herbicida BR112018077303A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2016-132689 2016-07-04
JP2016132689 2016-07-04
PCT/JP2017/024421 WO2018008617A1 (ja) 2016-07-04 2017-07-04 除草剤耐性を有する形質転換植物

Publications (1)

Publication Number Publication Date
BR112018077303A2 true BR112018077303A2 (pt) 2019-04-24

Family

ID=60912721

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018077303-4A BR112018077303A2 (pt) 2016-07-04 2017-07-04 planta transgênica com resistência a herbicida

Country Status (13)

Country Link
US (2) US11198710B2 (pt)
EP (2) EP3479685A4 (pt)
JP (1) JP7010819B2 (pt)
KR (2) KR102383502B1 (pt)
CN (1) CN109661172A (pt)
AR (1) AR108940A1 (pt)
AU (1) AU2017291543B2 (pt)
BR (1) BR112018077303A2 (pt)
CA (2) CA3029805A1 (pt)
MX (1) MX2018015720A (pt)
PH (1) PH12018502662A1 (pt)
TW (2) TWI812592B (pt)
WO (1) WO2018008617A1 (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR108940A1 (es) * 2016-07-04 2018-10-10 Kumiai Chemical Industry Co Planta transgénica que tiene resistencia a herbicidas
CN112939886B (zh) * 2021-02-04 2022-05-31 山西大学 一种可检测GSTs含量的近红外荧光探针及其合成方法和应用
CN113604488B (zh) * 2021-07-27 2023-03-14 中国林业科学研究院林业研究所 一种牡丹中参与花青苷转运和积累的gst基因及其应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MA20977A1 (fr) * 1986-05-19 1987-12-31 Ciba Geigy Ag Plantes tolerant les herbicides contenant le gene de gluthathione S-Transferase
US5073677A (en) * 1986-09-26 1991-12-17 Ciba-Geigy Corporation Herbicidal tolerant plants containing rat glutathione S-transferase gene
JP3810838B2 (ja) 1994-12-12 2006-08-16 三共アグロ株式会社 除草性イソオキサゾリン誘導体
JPH09328477A (ja) 1996-06-10 1997-12-22 Sankyo Co Ltd 除草性イソオキサゾリン誘導体
JPH09328483A (ja) 1996-06-11 1997-12-22 Sankyo Co Ltd 除草性イソオキサゾリン誘導体
GB9719727D0 (en) * 1997-09-16 1997-11-19 Rhone Poulenc Agriculture New plant genes
AU2924800A (en) * 1999-03-03 2000-09-21 Syngenta Limited Use of glutathione-s-transferase to increase stress tolerance in plants
US6841519B1 (en) 1999-08-10 2005-01-11 Kumiai Chemical Industry Co., Ltd. Isoxazoline derivatives and herbicides containing the same as the active ingredient
US7214786B2 (en) * 2000-12-14 2007-05-08 Kovalic David K Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement
JP4465133B2 (ja) 2001-02-08 2010-05-19 クミアイ化学工業株式会社 イソオキサゾリン誘導体及びこれを有効成分とする除草剤
NZ630693A (en) * 2013-02-22 2018-06-29 Dairy Australia Ltd Manipulation of self-incompatibility in plants
JP2016132689A (ja) 2015-01-16 2016-07-25 花王株式会社 手洗い用食器洗浄剤組成物
AR108940A1 (es) * 2016-07-04 2018-10-10 Kumiai Chemical Industry Co Planta transgénica que tiene resistencia a herbicidas

Also Published As

Publication number Publication date
JP7010819B2 (ja) 2022-02-10
CA3208377A1 (en) 2018-01-11
EP3479685A1 (en) 2019-05-08
KR20190024972A (ko) 2019-03-08
KR102383508B1 (ko) 2022-04-06
KR20220011813A (ko) 2022-01-28
US20190263874A1 (en) 2019-08-29
MX2018015720A (es) 2019-05-27
WO2018008617A1 (ja) 2018-01-11
CA3029805A1 (en) 2018-01-11
AU2017291543A1 (en) 2019-02-21
TW201803443A (zh) 2018-02-01
EP3479685A4 (en) 2020-07-29
CN109661172A (zh) 2019-04-19
JPWO2018008617A1 (ja) 2019-05-09
TWI812592B (zh) 2023-08-21
KR102383502B1 (ko) 2022-04-08
AU2017291543B2 (en) 2023-08-17
US20210395316A1 (en) 2021-12-23
US11198710B2 (en) 2021-12-14
TW202339612A (zh) 2023-10-16
AR108940A1 (es) 2018-10-10
EP4198128A1 (en) 2023-06-21
PH12018502662A1 (en) 2019-10-07

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