BR112019025744A8 - Método para aperfeiçoar a eficiência de transformação de planta e método para transformar planta - Google Patents

Método para aperfeiçoar a eficiência de transformação de planta e método para transformar planta

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Publication number
BR112019025744A8
BR112019025744A8 BR112019025744A BR112019025744A BR112019025744A8 BR 112019025744 A8 BR112019025744 A8 BR 112019025744A8 BR 112019025744 A BR112019025744 A BR 112019025744A BR 112019025744 A BR112019025744 A BR 112019025744A BR 112019025744 A8 BR112019025744 A8 BR 112019025744A8
Authority
BR
Brazil
Prior art keywords
plant
transformation efficiency
transform
improve
amino acid
Prior art date
Application number
BR112019025744A
Other languages
English (en)
Other versions
BR112019025744A2 (pt
Inventor
Ye Xingguo
Wang Ke
Ishida Yuji
Yanagihara Chizu
Liu Huiyun
Wang Kunyang
Shi Lei
Du Lipu
Wang Jing
Lin Zhishan
Original Assignee
Kaneka Corp
Inst Crop Science Caas
Japan Tobacco Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kaneka Corp, Inst Crop Science Caas, Japan Tobacco Inc filed Critical Kaneka Corp
Publication of BR112019025744A2 publication Critical patent/BR112019025744A2/pt
Publication of BR112019025744A8 publication Critical patent/BR112019025744A8/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cultivation Of Plants (AREA)
  • Peptides Or Proteins (AREA)

Abstract

A presente invenção fornece um método para melhorar a eficiência de transformação de uma planta. O método de acordo com a presente invenção compreende o uso de um ácido nucleico que codifique a sequência de aminoácidos descrita na SEQ ID NO: 2 ou 4 ou um ácido nucleico que codifique um polipeptídeo que compreende uma sequência de aminoácidos que tenha pelo menos 85% de identidade com a sequência de aminoácidos descrita na SEQ ID NO: 2 ou 4 e que tenha a função de melhorar a eficiência de transformação de uma planta.
BR112019025744A 2017-06-07 2018-06-07 Método para aperfeiçoar a eficiência de transformação de planta e método para transformar planta BR112019025744A8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201710422896.6 2017-06-07
CN201710422896.6A CN108997484B (zh) 2017-06-07 2017-06-07 小麦TaWox5基因在提高小麦转化效率中的应用
PCT/CN2018/090239 WO2018224001A1 (en) 2017-06-07 2018-06-07 Method for improving transformation efficiency of plant and method for transforming plant

Publications (2)

Publication Number Publication Date
BR112019025744A2 BR112019025744A2 (pt) 2020-06-30
BR112019025744A8 true BR112019025744A8 (pt) 2022-10-25

Family

ID=64566126

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112019025744A BR112019025744A8 (pt) 2017-06-07 2018-06-07 Método para aperfeiçoar a eficiência de transformação de planta e método para transformar planta

Country Status (7)

Country Link
US (1) US11447784B2 (pt)
EP (1) EP3619225A4 (pt)
CN (2) CN108997484B (pt)
AU (1) AU2018280528B2 (pt)
BR (1) BR112019025744A8 (pt)
CA (1) CA3064981A1 (pt)
WO (1) WO2018224001A1 (pt)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112521475B (zh) * 2020-12-25 2022-11-18 山东农业大学 小麦TaLAX1-A基因及其在提高小麦幼胚再生效率中的应用
EP4268583A1 (en) 2020-12-28 2023-11-01 Kaneka Corporation Method for producing transformed cells of corn or corn plant, nucleic acid construct, and method for introducing nucleic acid to cells or corn plant
CN112680539A (zh) * 2021-01-05 2021-04-20 中国农业科学院作物科学研究所 一种通过小麦TaMTL基因敲除突变体诱导的小麦单倍体植株筛选方法
CA3240766A1 (en) * 2021-12-22 2023-06-29 Timothy James Golds Use of tawox for improving regeneration of plant cells
WO2023118136A1 (en) 2021-12-22 2023-06-29 BASF Agricultural Solutions Seed US LLC Improved screening method for genome edited events
CN114736912B (zh) * 2022-03-24 2023-05-26 华南农业大学 优化的玉米rZmG2基因及其在提高植物遗传转化效率中的应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7256322B2 (en) 1999-10-01 2007-08-14 Pioneer Hi-Bred International, Inc. Wuschel (WUS) Gene Homologs
DE60034929T2 (de) * 1999-10-01 2008-01-24 E.I. Du Pont De Nemours And Co., Wilmington Wuschel (WUS)-Proteine
US20140130203A1 (en) * 2000-04-19 2014-05-08 Thomas J. La Rosa Rice nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement
JP2005185101A (ja) * 2002-05-30 2005-07-14 National Institute Of Agrobiological Sciences 植物の全長ｃＤＮＡおよびその利用
CN1536084A (zh) * 2003-04-09 2004-10-13 中国农业科学院作物育种栽培研究所 一种用农杆菌介导对小麦进行基因转化的方法
US8586824B2 (en) 2006-02-13 2013-11-19 Temasek Life Sciences Laboratory Plant homeodomain protein-encoding genes and their uses
WO2008001414A1 (fr) 2006-06-23 2008-01-03 Japan Tobacco Inc. Vecteur cosmide destiné à transformer une plante et son procédé d'utilisation
CN104232679A (zh) 2009-01-28 2014-12-24 巴斯夫植物科学有限公司 具有增强的产量相关性状的植物及其制备方法
JP4919305B2 (ja) 2010-03-12 2012-04-18 独立行政法人農業生物資源研究所 葉特異的発現活性を有するプロモーター
CN102533845B (zh) * 2011-12-31 2014-04-16 哈尔滨师范大学 一种农杆菌介导的小麦遗传转化方法
CN103224563B (zh) * 2013-03-29 2014-09-03 中国农业科学院作物科学研究所 合成型转录因子VP64- Os01g63510的应用
CN104031936A (zh) * 2014-06-16 2014-09-10 中国热带农业科学院橡胶研究所 一种转AtWUS基因促进橡胶树侧芽发生的方法
CN104263753A (zh) * 2014-10-24 2015-01-07 扬州大学 一种提高农杆菌介导法转化小麦的转化率的方法
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Also Published As

Publication number Publication date
CN111278849A (zh) 2020-06-12
US11447784B2 (en) 2022-09-20
CN111278849B (zh) 2022-11-15
CA3064981A1 (en) 2018-12-13
AU2018280528A8 (en) 2020-08-13
BR112019025744A2 (pt) 2020-06-30
AU2018280528B2 (en) 2022-08-25
EP3619225A1 (en) 2020-03-11
EP3619225A4 (en) 2020-12-30
WO2018224001A1 (en) 2018-12-13
CN108997484A (zh) 2018-12-14
AU2018280528A1 (en) 2019-12-19
CN108997484B (zh) 2020-07-24
US20200231977A1 (en) 2020-07-23

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Owner name: INSTITUTE OF CROP SCIENCE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES (CN) ; KANEKA CORPORATION (JP)