BRPI0511236A - método para a produção de ácidos graxos poliinsaturados com quatro ou mais ligações duplas, saccharomyces cerevisiae geneticamente modificado que tem capacidade de produzir ácidos graxos poliinsaturados com quatro ou mais ligações duplas quando desenvolvido em um substrato que não de ácido graxo, composição, uso da composição e uso de um saccharomyces cervisiae geneticamente modificado - Google Patents

método para a produção de ácidos graxos poliinsaturados com quatro ou mais ligações duplas, saccharomyces cerevisiae geneticamente modificado que tem capacidade de produzir ácidos graxos poliinsaturados com quatro ou mais ligações duplas quando desenvolvido em um substrato que não de ácido graxo, composição, uso da composição e uso de um saccharomyces cervisiae geneticamente modificado

Info

Publication number
BRPI0511236A
BRPI0511236A BRPI0511236-2A BRPI0511236A BRPI0511236A BR PI0511236 A BRPI0511236 A BR PI0511236A BR PI0511236 A BRPI0511236 A BR PI0511236A BR PI0511236 A BRPI0511236 A BR PI0511236A
Authority
BR
Brazil
Prior art keywords
producing
fatty acids
composition
double bond
genetically modified
Prior art date
Application number
BRPI0511236-2A
Other languages
English (en)
Inventor
Jochen Forster
Nina Katarina Gunnarsson
Jeans Bredal Nielsen
Original Assignee
Fluxome Sciences As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fluxome Sciences As filed Critical Fluxome Sciences As
Publication of BRPI0511236A publication Critical patent/BRPI0511236A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115—Fatty acids or derivatives thereof; Fats or oils
    • A23L33/12—Fatty acids or derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/36—Carboxylic acids; Salts or anhydrides thereof
    • A61K8/361—Carboxylic acids having more than seven carbon atoms in an unbroken chain; Salts or anhydrides thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67—Vitamins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6431—Linoleic acids [18:2[n-6]]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6481—Phosphoglycerides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

Abstract

MéTODO PARA A PRODUçãO DE áCIDOS GRAXOS POLIINSATURADOS COM QUATRO OU MAIS LIGAçõES DUPLAS, Saccharomyces cerevisiae GENETICAMENTE MODIFICADO QUE TEM CAPACIDADE DE PRODUZIR áCIDOS GRAXOS POLIINSATURADOS COM QUATRO OU MAIS LIGAçõES DUPLAS QUANDO DESENVOLVIDO EM UM SUBSTRATO QUE NãO DE áCIDO GRAXO, COMPOSIçãO, USO DA COMPOSIçãO E USO DE UM Saccharomyces cerevisiae GENETICAMENTE MODIFICADO A presente invenção refere-se à construção e à engenharia de células, mais particularmente de microorganismos para produzir PUFAs com quatro ou mais ligações duplas a partir de substratos que não de ácido graxo através da expressão heteróloga de uma passagem que requer oxigênio. A invenção envolve especialmente o aumento do teor de PUFA no organismo hospedeiro através da otimização da fermentação, por exemplo, ao diminuir a temperatura e/ou ao projetar um meio mais favorável ou através de aumento do fluxo em direção a ácidos graxos pela engenharia metabólica, por exemplo, através da superexpressão de sintases de ácido graxo, da superexpressão de outras enzimas envolvidas na biossintese do precursor para PUFAs ou da otimização de códons dos genes heterólogos ou da a expressão das enzimas heterólogas envolvidas na biossíntese do precursor para PUFAs.
BRPI0511236-2A 2004-06-04 2005-06-03 método para a produção de ácidos graxos poliinsaturados com quatro ou mais ligações duplas, saccharomyces cerevisiae geneticamente modificado que tem capacidade de produzir ácidos graxos poliinsaturados com quatro ou mais ligações duplas quando desenvolvido em um substrato que não de ácido graxo, composição, uso da composição e uso de um saccharomyces cervisiae geneticamente modificado BRPI0511236A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US57724504P 2004-06-04 2004-06-04
PCT/DK2005/000372 WO2005118814A2 (en) 2004-06-04 2005-06-03 Metabolically engineered cells for the production of polyunsaturated fatty acids

Publications (1)

Publication Number Publication Date
BRPI0511236A true BRPI0511236A (pt) 2007-11-27

Family

ID=34956349

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0511236-2A BRPI0511236A (pt) 2004-06-04 2005-06-03 método para a produção de ácidos graxos poliinsaturados com quatro ou mais ligações duplas, saccharomyces cerevisiae geneticamente modificado que tem capacidade de produzir ácidos graxos poliinsaturados com quatro ou mais ligações duplas quando desenvolvido em um substrato que não de ácido graxo, composição, uso da composição e uso de um saccharomyces cervisiae geneticamente modificado

Country Status (14)

Country Link
US (1) US7736884B2 (pt)
EP (1) EP1766023B1 (pt)
JP (1) JP4955540B2 (pt)
AT (1) ATE479757T1 (pt)
AU (1) AU2005250074B2 (pt)
BR (1) BRPI0511236A (pt)
CA (1) CA2568689A1 (pt)
DE (1) DE602005023308D1 (pt)
DK (1) DK1766023T3 (pt)
ES (1) ES2351973T3 (pt)
NZ (1) NZ552254A (pt)
PL (1) PL1766023T3 (pt)
PT (1) PT1766023E (pt)
WO (1) WO2005118814A2 (pt)

Families Citing this family (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2534961A1 (en) * 2003-03-10 2012-12-19 POET Research, Inc. Method for producing ethanol using raw starch
US20050233030A1 (en) 2004-03-10 2005-10-20 Broin And Associates, Inc. Methods and systems for producing ethanol using raw starch and fractionation
CA2535310C (en) 2003-08-21 2015-06-09 Monsanto Technology Llc Fatty acid desaturases from primula
CN1968688A (zh) 2004-04-16 2007-05-23 孟山都技术有限公司 脂肪酸去饱和酶在玉米中的表达
EP2357244A3 (en) 2004-04-22 2011-11-23 Commonwealth Scientific and Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells.
ES2529572T3 (es) 2004-04-22 2015-02-23 Commonwealth Scientific And Industrial Research Organisation Síntesis de ácidos grasos poliinsaturados de cadena larga por células recombinantes
CA2566252A1 (en) * 2004-05-13 2005-12-01 Novozymes North America, Inc. A process of producing a fermentation product
JP4587451B2 (ja) * 2004-08-20 2010-11-24 サントリーホールディングス株式会社 ω３脂肪酸不飽和化活性を有するポリペプチドおよびそのポリペプチドをコードするポリヌクレオチドならびにそれらの利用
GB0503657D0 (en) 2005-02-22 2005-03-30 Fluxome Sciences As Metabolically engineered cells for the production of resveratrol or an oligomeric or glycosidically-bound derivative thereof
DE102005038036A1 (de) 2005-08-09 2007-02-15 Basf Plant Science Gmbh Verfahren zur Herstellung von Arachidonsäure und/oder Eicosapentaensäure in transgenen Nutzpflanzen
US7919289B2 (en) 2005-10-10 2011-04-05 Poet Research, Inc. Methods and systems for producing ethanol using raw starch and selecting plant material
GB0603160D0 (en) * 2006-02-16 2006-03-29 Rothamsted Res Ltd Nucleic acid
AU2013219144B2 (en) * 2006-02-16 2016-08-04 Basf Plant Science Gmbh Nucleic acid
US7695950B2 (en) * 2006-05-17 2010-04-13 E. I. Du Pont De Nemours And Company Δ5 desaturase and its use in making polyunsaturated fatty acids
WO2008000277A2 (en) * 2006-06-29 2008-01-03 Fluxome Sciences A/S Metabolically engineered fungal cells with increased content of polyunsaturated fatty acids
AU2007275316B2 (en) * 2006-07-19 2012-09-06 Monsanto Technology Llc Fatty acid desaturases from Tetraselmis suecica
GB0614442D0 (en) 2006-07-20 2006-08-30 Fluxome Sciences As Metabolically engineered cells for the production of pinosylvin
SG10201600157QA (en) 2006-07-21 2016-02-26 Xyleco Inc Conversion systems for biomass
US9029111B2 (en) 2006-08-24 2015-05-12 Basf Plant Science Gmbh Isolation and characterization of a novel pythium omega 3 desaturase with specificity to all omega 6 fatty acids longer than 18 carbon chains
CA2661697A1 (en) 2006-08-29 2008-03-06 Commonwealth Scientific And Industrial Research Organisation Synthesis of fatty acids
WO2008073275A2 (en) 2006-12-07 2008-06-19 E. I. Du Pont De Nemours And Company Mutant delta-8 desaturase genes engineered by targeted mutagensis and their use in making polyunsaturated fatty acids
US7709239B2 (en) * 2006-12-07 2010-05-04 E.I. Du Pont De Nemours And Company Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
US8957280B2 (en) 2007-05-03 2015-02-17 E. I. Du Pont De Nemours And Company Delta-5 desaturases and their use in making polyunsaturated fatty acids
JP2010533003A (ja) * 2007-07-13 2010-10-21 オーシャン ニュートリッション カナダ リミテッド Ｄ４デサチュラーゼ及びｄ５エロンガーゼ
KR101519680B1 (ko) * 2007-07-23 2015-05-12 산토리 홀딩스 가부시키가이샤 신규 지방산 조성을 갖는 지방산 조성물
AU2008314885B2 (en) * 2007-10-26 2013-11-07 Suntory Holdings Limited Novel ATP:citrate lyase genes
US8343753B2 (en) 2007-11-01 2013-01-01 Wake Forest University School Of Medicine Compositions, methods, and kits for polyunsaturated fatty acids from microalgae
CA2704371A1 (en) * 2007-11-01 2009-05-07 Wake Forest University School Of Medicine Compositions and methods for prevention and treatment of mammalian diseases
GB0725075D0 (en) * 2007-12-21 2008-01-30 Fluxome Sciences As Metabolically engineered fungal cells with increased content of polyunsaturated fatty acids
EP2108702A1 (en) * 2008-04-07 2009-10-14 Organo Balance GmbH Synthesis of polyunsaturated fatty acids in yeast
US8597931B2 (en) 2008-07-11 2013-12-03 Institut National De La Recherche Agronomique (Inra) Mutant yeast strains capable of accumulating a large quantity of lipids
AR074364A1 (es) 2008-11-18 2011-01-12 Commw Scient Ind Res Org Enzimas y metodo para producir acidos grasos omega -3
US8802924B2 (en) * 2008-12-09 2014-08-12 Ben Gurion University Of The Negev Research And Development Authority Polyunsaturated fatty acid elongase
US20100233771A1 (en) * 2009-03-03 2010-09-16 Mcdonald William F System for pre-treatment of biomass for the production of ethanol
US9068206B1 (en) 2009-03-03 2015-06-30 Poet Research, Inc. System for treatment of biomass to facilitate the production of ethanol
HUE025623T2 (en) * 2009-03-03 2016-04-28 Poet Res Inc A method of fermenting biomass for ethanol production
US8450094B1 (en) * 2009-03-03 2013-05-28 Poet Research, Inc. System for management of yeast to facilitate the production of ethanol
US9920303B2 (en) * 2009-11-20 2018-03-20 Bayer Cropscience Nv Brassica plants comprising mutant FAD3 alleles
EP2327776A1 (en) 2009-11-30 2011-06-01 Institut National De La Recherche Agronomique Method for the production of Very Long Chain Fatty Acids (VLCFA) by fermentation with a recombinant Yarrowia sp
CN102947440B (zh) 2009-12-30 2015-04-08 埃欧金能源公司 呈现增强的木质纤维素水解产物发酵的改良酵母菌株
BR112012022108B1 (pt) 2010-03-02 2022-01-18 Massachusetts Institute Technology Célula de levedura oleaginosa, cultura, bem como métodos para converter uma fonte de carboidrato em ácido graxo ou triacilglicerol e para modificar uma célula de levedura
WO2011133610A1 (en) 2010-04-22 2011-10-27 E. I. Du Pont De Nemours And Company Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass
GB201008826D0 (en) 2010-05-26 2010-07-14 Fluxome Sciences As Production of metabolites
KR20180008885A (ko) * 2010-06-24 2018-01-24 다우 아그로사이언시즈 엘엘씨 식물 종자의 포화 지방산 함량의 저하
FR2962133B1 (fr) * 2010-07-01 2014-09-12 Agronomique Inst Nat Rech Optimisation de la synthese et de l'accumulation de lipides
DK2601292T3 (en) * 2010-08-06 2018-07-16 Biopetrolia Ab METHODS AND PRODUCTS FOR MANUFACTURING GROWERS
WO2012037215A2 (en) * 2010-09-15 2012-03-22 Wisconsin Alumni Research Foundation Recombinant yeast with improved ethanol tolerance and related methods of use
BR112013023060A2 (pt) * 2011-03-08 2016-12-13 Poet Res Inc sistemas e métodos para o melhoramento da destilação
US20120247066A1 (en) 2011-04-01 2012-10-04 Ice House America, Llc Ice bagging apparatus and methods
CN102220351A (zh) * 2011-04-04 2011-10-19 山东农业大学 马铃薯致病疫霉△5去饱和酶基因PinD5的功能鉴定与应用
US8785164B2 (en) 2011-05-26 2014-07-22 E I Du Pont De Nemours And Company Expression of caleosin in recombinant oleaginous microorganisms to increase oil content therein
US8951776B2 (en) * 2011-10-19 2015-02-10 Massachusetts Institute Of Technology Engineered microbes and methods for microbial oil production
US8790901B2 (en) 2011-12-14 2014-07-29 Pronutria, Inc. Microorganisms and methods for producing unsaturated fatty acids
CN102660576B (zh) * 2012-04-12 2014-06-11 中国人民解放军军事医学科学院生物工程研究所 一种在哺乳动物细胞中生产dha的方法
SG11201408362SA (en) 2012-06-15 2015-01-29 Commw Scient Ind Res Org Production of long chain polyunsaturated fatty acids in plant cells
CN102787128A (zh) * 2012-08-28 2012-11-21 江南大学 一种用于多不饱和脂肪酸生物合成的脱饱和酶Δ9 Des
NZ709083A (en) 2012-12-24 2020-07-31 Qualitas Health Inc Eicosapentaenoic acid (epa) formulations
US9340767B2 (en) 2013-03-13 2016-05-17 Poet Research, Inc. Propagating an organism and related methods and compositions
US9034631B2 (en) 2013-03-14 2015-05-19 Poet Research, Inc. Systems and methods for yeast propagation
EP3004325A4 (en) * 2013-05-28 2017-01-18 POET Research, Inc. System for management of yeast to facilitate the production of ethanol
BR112016003256B1 (pt) 2013-08-27 2022-10-11 Ginkgo Bioworks, Inc. Microorganismo geneticamente modificado que metaboliza fontes não convencionais de fósforo ou enxofre e método para converter um subtrato em um produto
KR20210134820A (ko) 2013-12-05 2021-11-10 게노마티카 인코포레이티드 지방족 아민의 미생물 생성
CN111154724B (zh) 2013-12-18 2024-02-06 联邦科学技术研究组织 包含二十二碳六烯酸的提取的植物脂质
US9752165B2 (en) * 2014-02-10 2017-09-05 Cellulosic Ethanol Technologies, Llc Processes and systems for recovering oil from fermentation products
SG11201610596PA (en) 2014-06-27 2017-01-27 Commw Scient Ind Res Org Lipid comprising docosapentaenoic acid
EP3218499B1 (en) 2014-11-14 2021-03-10 Basf Plant Science Company GmbH Brassica events lbflfk and lbfdau and methods for detection thereof
CA2987508A1 (en) 2015-05-28 2016-12-01 Evolva Sa Biosynthesis of phenylpropanoids and phenylpropanoid derivatives
US10513718B2 (en) 2017-06-06 2019-12-24 City University Of Hong Kong Method of producing polyunsaturated fatty acid
WO2019118869A1 (en) 2017-12-14 2019-06-20 Poet Research, Inc. Method for propagating microorganisms on a medium comprising stillage
CN109234179B (zh) * 2018-10-29 2021-11-12 贵州茅台酒股份有限公司 一种酒醅中酵母菌分离培养基及其制备方法
BR112021008842A2 (pt) * 2018-11-09 2021-08-17 Evonik Operations Gmbh método para produzir uma biomassa com um teor aumentado de ácidos graxos poli-insaturados
CN109929870B (zh) * 2019-02-20 2021-03-16 天津大学 糖代谢与脂质代谢协同提高解脂耶氏酵母合成脂肪酸衍生物的产量的应用
CN111218409A (zh) * 2019-11-27 2020-06-02 江西科技师范大学 一种耐高盐的酿酒酵母菌株、其构建方法及应用
CN116034164A (zh) * 2020-03-31 2023-04-28 倍耐力轮胎股份公司 工程改造含油微生物的方法
CN112481147B (zh) * 2020-12-10 2022-04-29 科稷达隆(北京)生物技术有限公司 一种谷氨酰胺合成酶基因功能缺失的酵母突变体及其制备方法和应用
US20240117377A1 (en) * 2021-02-02 2024-04-11 Allen Institute Artificial expression constructs for modulating gene expression in gabaergic neurons and astrocytes
CN116121092A (zh) * 2022-11-01 2023-05-16 四川大学 多重胁迫抗性增强的重组酿酒酵母菌及其构建方法与应用

Family Cites Families (82)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1982001011A1 (en) 1980-09-24 1982-04-01 Corp Cetus Diagnostic method and probe
US4582788A (en) * 1982-01-22 1986-04-15 Cetus Corporation HLA typing method and cDNA probes used therein
EP0084796B1 (en) 1982-01-22 1990-05-02 Cetus Corporation Hla typing method and cdna probes used therein
US4683194A (en) * 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
CA1338457C (en) 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US5057419A (en) * 1988-09-22 1991-10-15 Rutgers University Genetically engineered plasmid and organisms for the production of specialized oils
US6355861B1 (en) * 1991-10-10 2002-03-12 Rhone-Poulenc Agrochimie Production of gamma linolenic acid by a Δ6-desaturase
CA2092661C (en) * 1992-03-13 2006-11-07 Candace Gloria Poutre Modification of vegetable oils using desaturase
US6872872B1 (en) * 1992-11-17 2005-03-29 E. I. Du Pont De Nemours And Company Genes for microsomal delta-12 fatty acid desaturases and related enzymes from plants
US6372965B1 (en) * 1992-11-17 2002-04-16 E.I. Du Pont De Nemours And Company Genes for microsomal delta-12 fatty acid desaturases and hydroxylases from plants
DE69333025T2 (de) 1992-11-17 2004-04-29 E.I. Du Pont De Nemours And Co., Wilmington Für mikrosom delta 12 fettsäuredesaturase kodierende gene und verwandte enzyme von pflanzen
US5869304A (en) * 1994-10-12 1999-02-09 University Of Kentucky Research Foundation Technique for specifying the fatty acid at the sn2 position of acylglycerol lipids
GB9510927D0 (en) * 1995-05-31 1995-07-26 Ca Nat Research Council Plant and seed oil modification
EP0794250A1 (en) * 1996-03-04 1997-09-10 Soremartec S.A. Isolation and sequencing of the hazel FAd2-N gene
US5850026A (en) * 1996-07-03 1998-12-15 Cargill, Incorporated Canola oil having increased oleic acid and decreased linolenic acid content
US6194167B1 (en) * 1997-02-18 2001-02-27 Washington State University Research Foundation ω-3 fatty acid desaturase
US6432684B1 (en) 1997-04-11 2002-08-13 Abbott Laboratories Human desaturase gene and uses thereof
US5968809A (en) * 1997-04-11 1999-10-19 Abbot Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US6428990B1 (en) * 1997-04-11 2002-08-06 Abbott Laboratories Human desaturase gene and uses thereof
DK0996732T3 (da) 1997-04-11 2005-10-17 Calgene Llc Fremgangsmåder og sammensætninger til syntese af langkædede polyumættede fedtsyrer i planter
US5972664A (en) 1997-04-11 1999-10-26 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
WO1999011806A1 (en) * 1997-09-04 1999-03-11 Rutgers, The State University Of New Jersey Pathogen-resistant transgenic plants and methods of making
GB9724783D0 (en) 1997-11-24 1998-01-21 Inst Arable Crops Research Novel polypeptides
CN1283230A (zh) 1997-12-23 2001-02-07 布利斯脱大学 去饱和酶
US6492108B1 (en) * 1998-03-26 2002-12-10 Incyte Genomics, Inc. Delta-6 desaturase homologs
US6825335B1 (en) * 1998-08-24 2004-11-30 Rutgers, The State University Of New Jersey Synthetic fatty acid desaturase gene for expression in plants
US6677145B2 (en) * 1998-09-02 2004-01-13 Abbott Laboratories Elongase genes and uses thereof
US20030163845A1 (en) 1998-09-02 2003-08-28 Pradip Mukerji Elongase genes and uses thereof
US6403349B1 (en) * 1998-09-02 2002-06-11 Abbott Laboratories Elongase gene and uses thereof
US6913916B1 (en) * 1998-09-02 2005-07-05 Abbott Laboratories Elongase genes and uses thereof
WO2000034439A1 (en) 1998-12-07 2000-06-15 Washington State University Research Foundation Desaturases and methods of using them for synthesis of polyunsaturated fatty acids
US6686186B2 (en) * 1999-01-19 2004-02-03 Brookhaven Science Associates Llc Mutant fatty acid desaturase
US20020151019A1 (en) * 1999-01-19 2002-10-17 John Shanklin Mutant fatty acid desaturase and methods for directed mutagenesis
EP1161542A1 (en) * 1999-03-18 2001-12-12 University Of Bristol Polysaturated fatty acid (pufa) elongase from caenorhabditis elegans
HUP0201204A3 (en) * 1999-05-21 2004-07-28 Cargill Dow Llc Minnetonka Methods and materials for synthesis of organic products
WO2001002591A1 (de) 1999-07-06 2001-01-11 Basf Plant Science Gmbh Δ6-desaturasegene exprimierende pflanzen und pufas enthaltende öle aus diesen pflanzen und ein verfahren zur herstellung ungesättigter fettsäuren
WO2001004636A1 (en) 1999-07-12 2001-01-18 Ohio University Mammalian cells expressing desaturases and elongases
US7070970B2 (en) * 1999-08-23 2006-07-04 Abbott Laboratories Elongase genes and uses thereof
DE60037735T2 (de) 1999-08-26 2009-01-02 Calgene Llc, Davis Pflanzen mit veränderten mehrfachungesättigten fettsäuren
FR2798391B1 (fr) 1999-09-15 2001-12-14 Inst Nat Sante Rech Med Enzyme thyroidienne nadph oxydase, acide nucleique codant pour cette enzyme et leurs applications
DE19950589A1 (de) * 1999-10-20 2001-05-23 Gvs Ges Fuer Erwerb Und Verwer Elongasepromotoren für gewebespezifische Expression von Transgenen in Pflanzen
GB9929897D0 (en) 1999-12-18 2000-02-09 Slabas Antoni R Improvements in or relating to conjugated fatty acids and related compounds
US20030172398A1 (en) * 1999-12-21 2003-09-11 Browse John A. Novel delta-12 desaturase and methods of using it for synthesis of polyunsaturated fatty acids
EE200200443A (et) * 2000-02-09 2003-12-15 Basf Aktiengesellschaft Uus elongaasigeen ning polüküllastumata rasvhapete saamise meetod
AU2000234588A1 (en) 2000-03-31 2001-10-15 Idemitsu Petrochemical Co. Ltd. Process for producing lipids and lipid-secreting microorganisms
US6974898B2 (en) 2000-04-18 2005-12-13 Commonwealth Scientific And Industrial Research Organisation Method of modifying the content of cottonseed oil
WO2001085968A2 (en) 2000-05-09 2001-11-15 Bioriginal Food & Science Corp. Production of conjugated linoleic and linolenic acids in plants
DK1911837T3 (da) 2000-09-28 2011-08-29 Bioriginal Food & Science Corp FAD5-2 fedtsyredesaturasefamiliemedlem og anvendelser deraf
JP2004512049A (ja) 2000-10-26 2004-04-22 キセノン　ジェネティックス　インコーポレイテッド 脂質代謝を調節する化合物をスクリーニングする方法
WO2002042471A2 (en) * 2000-11-22 2002-05-30 Cargill Dow Polymers Llc Methods and materials for the synthesis of organic products
AU2002221404B2 (en) 2000-11-29 2008-01-03 Xenon Genetics Inc Human elongase genes and uses thereof
DE10102337A1 (de) * 2001-01-19 2002-07-25 Basf Plant Science Gmbh Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren, neue Biosynthesegene sowie neue pflanzliche Expressionskonstrukte
DE10102338A1 (de) * 2001-01-19 2002-07-25 Basf Plant Science Gmbh Verfahren zur Expression von Biosynthesegenen in pflanzlichen Samen unter Verwendung von neuen multiplen Expressionskonstrukten
CA2435685C (en) 2001-01-25 2011-06-07 Abbott Laboratories Desaturase genes and uses thereof
US6635451B2 (en) * 2001-01-25 2003-10-21 Abbott Laboratories Desaturase genes and uses thereof
WO2002070659A2 (en) * 2001-03-02 2002-09-12 Regents Of The University Of Minnesota Production of polyhydroxyalkanoates
AU2002258505A1 (en) 2001-03-12 2002-09-24 The General Hospital Corporation Compositions and methods for modifying the content of polyunsaturated fatty acids in mammalian cells
GB0107510D0 (en) 2001-03-26 2001-05-16 Univ Bristol New elongase gene and a process for the production of -9-polyunsaturated fatty acids
WO2002081702A1 (en) 2001-04-05 2002-10-17 Idemitsu Technofine Co., Ltd. Fatty acid δ6-position desaturase genes and plasmids and tra nsformant containing these genes
US7045683B2 (en) 2001-05-04 2006-05-16 Abbott Laboratories Δ4-desaturase genes and uses thereof
WO2002102970A2 (en) * 2001-06-06 2002-12-27 Bioriginal Food & Science Corporation Flax (linum usitatissimim l.) seed-specific promoters
DE10137374A1 (de) 2001-07-31 2003-02-27 Ipk Inst Fuer Pflanzengenetik Verfahren zur Herstellung von Arachidonsäure in transgenen Organismen
US6875595B2 (en) * 2001-09-13 2005-04-05 Divergence, Inc. Nematode fatty acid desaturase-like sequences
US7211656B2 (en) * 2002-01-30 2007-05-01 Abbott Laboratories Desaturase genes, enzymes encoded thereby, and uses thereof
BR0306883A (pt) 2002-01-30 2004-12-07 Basf Plant Science Gmbh ácido nucléico isolado, sequências de ácido nucléico isolado e de aminoácido, construção de gene, vetor, organismo, processo para a produção de pufas, óleo, lipìdeo ou ácido graxo ou uma fração destes, composição de óleo, lipìdeo ou de ácido graxo, e, uso da mesma
GB2385852A (en) 2002-02-27 2003-09-03 Rothamsted Ex Station Delta 6-desaturases from Primulaceae
US7700833B2 (en) * 2002-03-01 2010-04-20 Cornell University Process for the production of unsaturated fatty acids
WO2004005442A1 (de) * 2002-07-03 2004-01-15 Basf Plant Science Gmbh Verfahren zur herstellung von konjugierten mehrfach ungesättigten fettsäuren mit mindestens zwei doppelbindungen in pflanzen
ES2204328B1 (es) * 2002-10-04 2005-09-01 Consejo Sup. De Invest. Cientificas Procedimiento de produccion de acidos grasos polinsaturados con levaduras por incorporacion de sustratos olefinicos o acetilenicos.
AU2003296638B2 (en) * 2002-12-19 2009-06-11 University Of Bristol Method for the production of polyunsaturated fatty acids
AU2004215705B2 (en) * 2003-02-27 2009-11-12 Basf Plant Science Gmbh Method for the production of polyunsaturated fatty acids
US7125672B2 (en) * 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7214491B2 (en) * 2003-05-07 2007-05-08 E. I. Du Pont De Nemours And Company Δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts
US7238482B2 (en) * 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7259255B2 (en) * 2003-06-25 2007-08-21 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US7267976B2 (en) * 2003-07-02 2007-09-11 E.I. Du Pont De Nemours And Company Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts
US7504259B2 (en) * 2003-11-12 2009-03-17 E. I. Du Pont De Nemours And Company Δ12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast
US7465564B2 (en) * 2004-08-10 2008-12-16 E.I. Du Pont De Nemours And Company Production of γ-linolenic acid in oleaginous yeast
US7198937B2 (en) * 2004-11-04 2007-04-03 E. I. Du Pont De Nemours And Company Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7550286B2 (en) * 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
US7273746B2 (en) * 2004-11-04 2007-09-25 E.I. Dupont De Nemours And Company Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms

Also Published As

Publication number Publication date
EP1766023B1 (en) 2010-09-01
PL1766023T3 (pl) 2011-04-29
DE602005023308D1 (de) 2010-10-14
JP4955540B2 (ja) 2012-06-20
ES2351973T3 (es) 2011-02-14
NZ552254A (en) 2008-12-24
WO2005118814A3 (en) 2006-03-09
US7736884B2 (en) 2010-06-15
ATE479757T1 (de) 2010-09-15
AU2005250074A1 (en) 2005-12-15
US20060051847A1 (en) 2006-03-09
PT1766023E (pt) 2010-12-09
EP1766023A2 (en) 2007-03-28
CA2568689A1 (en) 2005-12-15
AU2005250074B2 (en) 2011-04-14
WO2005118814A2 (en) 2005-12-15
WO2005118814A9 (en) 2006-02-09
JP2008501323A (ja) 2008-01-24
DK1766023T3 (da) 2010-12-13

Similar Documents

Publication Publication Date Title
BRPI0511236A (pt) método para a produção de ácidos graxos poliinsaturados com quatro ou mais ligações duplas, saccharomyces cerevisiae geneticamente modificado que tem capacidade de produzir ácidos graxos poliinsaturados com quatro ou mais ligações duplas quando desenvolvido em um substrato que não de ácido graxo, composição, uso da composição e uso de um saccharomyces cervisiae geneticamente modificado
Spagnuolo et al. Oleaginous yeast for biofuel and oleochemical production
Athenaki et al. Lipids from yeasts and fungi: physiology, production and analytical considerations
Zhang et al. Enhanced lipid accumulation in the yeast Yarrowia lipolytica by over-expression of ATP: citrate lyase from Mus musculus
Kamisaka et al. DGA1 (diacylglycerol acyltransferase gene) overexpression and leucine biosynthesis significantly increase lipid accumulation in the Δ snf2 disruptant of Saccharomyces cerevisiae
Liu et al. Frontiers of yeast metabolic engineering: diversifying beyond ethanol and Saccharomyces
Liu et al. Sesamol enhances cell growth and the biosynthesis and accumulation of docosahexaenoic acid in the microalga Crypthecodinium cohnii
Huang et al. Expression and characterization of CYP52 genes involved in the biosynthesis of sophorolipid and alkane metabolism from Starmerella bombicola
Kamisaka et al. Overexpression of the active diacylglycerol acyltransferase variant transforms Saccharomyces cerevisiae into an oleaginous yeast
Guo et al. Improvement of lipid production in oleaginous yeast Rhodosporidium toruloides by ultraviolet mutagenesis
Werner et al. Biotechnological production of bio-based long-chain dicarboxylic acids with oleogenious yeasts
JP2008530994A5 (pt)
Zhang et al. Effects of carbon dioxide on cell growth and propionic acid production from glycerol and glucose by Propionibacterium acidipropionici
Shah et al. Microbes: A hidden treasure of polyunsaturated fatty acids
Huang et al. Combined “de novo” and “ex novo” lipid fermentation in a mix-medium of corncob acid hydrolysate and soybean oil by Trichosporon dermatis
Shi et al. Identification of a novel C22-∆ 4-producing docosahexaenoic acid (DHA) specific polyunsaturated fatty acid desaturase gene from Isochrysis galbana and its expression in Saccharomyces cerevisiae
Nozawa Adaptive regulation of membrane lipids and fluidity during thermal acclimation in Tetrahymena
Yilmaz et al. Determination of substrate preferences for desaturases and elongases for production of docosahexaenoic acid from oleic acid in engineered canola
Liu et al. Engineering yeast phospholipid metabolism for de novo oleoylethanolamide production
Wen et al. Continuous cultivation of the diatom Nitzschia laevis for eicosapentaenoic acid production: physiological study and process optimization
Lee et al. Ether phospholipids govern ferroptosis
Livore et al. Elongation of polyunsaturated fatty acids in trypanosomatids
Zhao et al. Very long chain polyunsaturated fatty acids accumulated in triacylglycerol are channeled from phosphatidylcholine in Thraustochytrium
Ishibashi et al. PUFA synthase-independent DHA synthesis pathway in Parietichytrium sp. and its modification to produce EPA and n-3DPA
Cheawchanlertfa et al. Up-regulated expression of desaturase genes of Mucor rouxii in response to low temperature associates with pre-existing cellular fatty acid constituents

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 9A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2257 DE 08/04/2014.