BR9912680A - Método de produção de uma cultura de ceral transformada, variedadde de milho, amido isolado do grão de uma variedade de milho, farinha preparada a partir de uma variedade de cultura de cereal, método de preparação de um composto alimentar espessado, variedade de milho transformada com um gene quimérico e amido - Google Patents

Método de produção de uma cultura de ceral transformada, variedadde de milho, amido isolado do grão de uma variedade de milho, farinha preparada a partir de uma variedade de cultura de cereal, método de preparação de um composto alimentar espessado, variedade de milho transformada com um gene quimérico e amido

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Publication number
BR9912680A
BR9912680A BR9912680-0A BR9912680A BR9912680A BR 9912680 A BR9912680 A BR 9912680A BR 9912680 A BR9912680 A BR 9912680A BR 9912680 A BR9912680 A BR 9912680A
Authority
BR
Brazil
Prior art keywords
variety
corn
starch
cereal
grain
Prior art date
Application number
BR9912680-0A
Other languages
English (en)
Inventor
Karem E Broglie
Jonathan Edward Lightner
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of BR9912680A publication Critical patent/BR9912680A/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B30/00—Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Polymers & Plastics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Materials Engineering (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

<B>"MéTODO DE PRODUçãO DE UMA CULTURA DE CEREAL TRANSFORMADA, VARIEDADE DE MILHO, AMIDO ISOLADO DO GRãO DE UMA VARIEDADE DE MILHO, FARINHA PREPARADA A PARTIR DE UMA VARIEDADE DE CULTURA DE CEREAL, MéTODO DE PREPARAçãO DE UM COMPOSTO ALIMENTAR ESPESSADO, VARIEDADE DE MILHO TRANSFORMADA COM UM GENE QUIMéRICO E AMIDO"<D> A presente invenção apresenta a utilização de um clone de cDNA para construir genes com sentido e anti-sentido para a inibição da atividade enzimática da amido sintase no milho. Mais especificamente, a presente invenção se refere a um método de controle da estrutura fina do amido derivado do grão de culturas de cereais, o qual compreende: (1) a preparação de um gene quimérico que compreende um fragmento de ácido nucléico que codifica um gene estrutural de amido sintase ou um fragmento dele, operacionalmente ligado em orientação com sentido ou anti-sentido no lado anterior a um fragmento de ácido nucléico que codifica um promotor que dirige a expressão do gene em tecido de endosperma de milho, e operacionalmente ligado no lado posterior a um fragmento de ácido nucléico que codifica uma sequência reguladora adequada para a terminação da transcrição; e (2) a transformação de culturas de cereais com o dito gene quimérico, em que a expressão do dito gene quimérico resulta na alteração da estrutura fina do amido derivado do grão das ditas culturas de cereais transformadas, em comparação com a estrutura fina do amido derivado de culturas de cereais que não possuam o dito gene quimérico.
BR9912680-0A 1998-07-28 1999-07-26 Método de produção de uma cultura de ceral transformada, variedadde de milho, amido isolado do grão de uma variedade de milho, farinha preparada a partir de uma variedade de cultura de cereal, método de preparação de um composto alimentar espessado, variedade de milho transformada com um gene quimérico e amido BR9912680A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9443698P 1998-07-28 1998-07-28
PCT/US1999/016296 WO2000006755A2 (en) 1998-07-28 1999-07-26 Modification of starch biosynthetic enzyme gene expression to produce starches in grain crops

Publications (1)

Publication Number Publication Date
BR9912680A true BR9912680A (pt) 2001-10-09

Family

ID=22245195

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9912680-0A BR9912680A (pt) 1998-07-28 1999-07-26 Método de produção de uma cultura de ceral transformada, variedadde de milho, amido isolado do grão de uma variedade de milho, farinha preparada a partir de uma variedade de cultura de cereal, método de preparação de um composto alimentar espessado, variedade de milho transformada com um gene quimérico e amido

Country Status (11)

Country Link
US (2) US6392120B1 (pt)
EP (1) EP1100938A2 (pt)
JP (1) JP2002525029A (pt)
CN (1) CN1314946A (pt)
AR (1) AR020642A1 (pt)
AU (1) AU765823B2 (pt)
BR (1) BR9912680A (pt)
CA (1) CA2330213C (pt)
HU (1) HUP0104208A3 (pt)
RO (1) RO120921B1 (pt)
WO (1) WO2000006755A2 (pt)

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US7033627B2 (en) * 1990-03-23 2006-04-25 Syngenta Mogen B.V. Production of enzymes in seeds and their use
US6392120B1 (en) 1998-07-28 2002-05-21 E. I. Du Pont De Nemours And Company Modification of starch biosynthetic enzyme gene expression to produce starches in grain crops
AUPQ005299A0 (en) * 1999-04-29 1999-05-27 Commonwealth Scientific And Industrial Research Organisation Novel genes encoding wheat starch synthases and uses therefor
MXPA03001678A (es) * 2000-08-28 2003-09-10 Du Pont Nuevos almidones producidos mediante la expresion de genes de la sintasa de almidon enlazados a granulos heterologos.
US7244839B2 (en) 2002-03-27 2007-07-17 E. I. Du Pont De Nemours And Company Maize starch containing elevated amounts of actual amylose
US7812221B2 (en) 2003-06-30 2010-10-12 Commonwealth Scientific And Industrial Research Organization Wheat with altered branching enzyme activity and starch and starch containing products derived therefrom
CN1886507B (zh) 2003-10-27 2010-12-29 联邦科技产业研究组织 淀粉中具有增加了直链淀粉含量的水稻及水稻制品
GB0328154D0 (en) * 2003-12-04 2004-01-07 Plant Bioscience Ltd Nucleic acids having utility in seeds
CN100402664C (zh) * 2005-03-21 2008-07-16 中国科学院成都生物研究所 一种通过特异性dna片段识别水稻糯性基因的分子标记方法
AR054174A1 (es) * 2005-07-22 2007-06-06 Bayer Cropscience Gmbh Sobreexpresion de sintasa de almidon en vegetales
US7674897B2 (en) * 2005-09-09 2010-03-09 Tate & Lyle Ingredients Americas, Inc. Production of crystalline short chain amylose
AR064557A1 (es) * 2006-12-29 2009-04-08 Bayer Cropscience Ag Almidon de maiz y harinas y alimentos de maiz que comprenden este almidon de maiz
AU2008238018A1 (en) * 2007-04-13 2008-10-23 Basf Plant Science Gmbh Polynucleotides for regulation of high level tissue-preferred expression in crop plants
CN101665786B (zh) * 2008-09-05 2012-07-18 中国科学院上海生命科学研究院 调节块根植物淀粉组成的方法
WO2014066497A2 (en) * 2012-10-23 2014-05-01 Montana State University Production of high quality durum wheat having increased amylose content
US9988641B2 (en) * 2016-04-05 2018-06-05 Corn Products Development, Inc. Compositions and methods for producing starch with novel functionality
CN106337056B (zh) * 2016-09-23 2018-10-02 中国热带农业科学院热带生物技术研究所 一种改良植物果实支链淀粉品质的基因及其编码产物与应用

Family Cites Families (11)

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Publication number Priority date Publication date Assignee Title
US5300145B1 (en) * 1992-08-28 1995-11-28 Nat Starch Chem Invest Low amylopectin starch
CA2146998A1 (en) 1992-10-14 1994-04-28 Colin Roger Bird Novel plants and processes for obtaining them
US5824790A (en) 1994-06-21 1998-10-20 Zeneca Limited Modification of starch synthesis in plants
GB9524938D0 (en) 1995-12-06 1996-02-07 Zeneca Ltd Modification of starch synthesis in plants
AU1684697A (en) 1995-12-20 1997-07-14 E.I. Du Pont De Nemours And Company Novel starches via modification of expression of starch biosynthetic enzyme genes
DE19601365A1 (de) 1996-01-16 1997-07-17 Planttec Biotechnologie Gmbh Nucleinsäuremoleküle aus Pflanzen codierend Enzyme, die an der Stärkesynthese beteiligt sind
DE19619918A1 (de) 1996-05-17 1997-11-20 Planttec Biotechnologie Gmbh Nucleinsäuremoleküle codierend lösliche Stärkesynthasen aus Mais
DE69737448T2 (de) 1996-05-29 2007-11-15 Bayer Cropscience Ag Nukleinsäuremoleküle, die für enzyme aus weizen kodieren, welche an der stärkesynthese beteiligt sind
EP0935665B1 (en) 1996-09-30 2008-03-19 BASF Plant Science GmbH Encapsulation of polypeptides within the starch matrix
WO1998044780A1 (en) 1997-04-04 1998-10-15 Exseed Genetics, Llc Plant like starches and the method of making them in hosts
US6392120B1 (en) 1998-07-28 2002-05-21 E. I. Du Pont De Nemours And Company Modification of starch biosynthetic enzyme gene expression to produce starches in grain crops

Also Published As

Publication number Publication date
US6392120B1 (en) 2002-05-21
CA2330213C (en) 2012-01-03
JP2002525029A (ja) 2002-08-13
CA2330213A1 (en) 2000-02-10
AU765823B2 (en) 2003-10-02
WO2000006755A2 (en) 2000-02-10
HUP0104208A3 (en) 2003-12-29
HUP0104208A2 (hu) 2002-03-28
RO120921B1 (ro) 2006-09-29
AR020642A1 (es) 2002-05-22
EP1100938A2 (en) 2001-05-23
CN1314946A (zh) 2001-09-26
US6570008B1 (en) 2003-05-27
WO2000006755A3 (en) 2000-09-08
AU5217499A (en) 2000-02-21

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1911 DE 21/08/2007.