BR9912680A - Método de produção de uma cultura de ceral transformada, variedadde de milho, amido isolado do grão de uma variedade de milho, farinha preparada a partir de uma variedade de cultura de cereal, método de preparação de um composto alimentar espessado, variedade de milho transformada com um gene quimérico e amido - Google Patents
Método de produção de uma cultura de ceral transformada, variedadde de milho, amido isolado do grão de uma variedade de milho, farinha preparada a partir de uma variedade de cultura de cereal, método de preparação de um composto alimentar espessado, variedade de milho transformada com um gene quimérico e amidoInfo
- Publication number
- BR9912680A BR9912680A BR9912680-0A BR9912680A BR9912680A BR 9912680 A BR9912680 A BR 9912680A BR 9912680 A BR9912680 A BR 9912680A BR 9912680 A BR9912680 A BR 9912680A
- Authority
- BR
- Brazil
- Prior art keywords
- variety
- corn
- starch
- cereal
- grain
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B30/00—Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Nutrition Science (AREA)
- Virology (AREA)
- Cell Biology (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Polymers & Plastics (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
<B>"MéTODO DE PRODUçãO DE UMA CULTURA DE CEREAL TRANSFORMADA, VARIEDADE DE MILHO, AMIDO ISOLADO DO GRãO DE UMA VARIEDADE DE MILHO, FARINHA PREPARADA A PARTIR DE UMA VARIEDADE DE CULTURA DE CEREAL, MéTODO DE PREPARAçãO DE UM COMPOSTO ALIMENTAR ESPESSADO, VARIEDADE DE MILHO TRANSFORMADA COM UM GENE QUIMéRICO E AMIDO"<D> A presente invenção apresenta a utilização de um clone de cDNA para construir genes com sentido e anti-sentido para a inibição da atividade enzimática da amido sintase no milho. Mais especificamente, a presente invenção se refere a um método de controle da estrutura fina do amido derivado do grão de culturas de cereais, o qual compreende: (1) a preparação de um gene quimérico que compreende um fragmento de ácido nucléico que codifica um gene estrutural de amido sintase ou um fragmento dele, operacionalmente ligado em orientação com sentido ou anti-sentido no lado anterior a um fragmento de ácido nucléico que codifica um promotor que dirige a expressão do gene em tecido de endosperma de milho, e operacionalmente ligado no lado posterior a um fragmento de ácido nucléico que codifica uma sequência reguladora adequada para a terminação da transcrição; e (2) a transformação de culturas de cereais com o dito gene quimérico, em que a expressão do dito gene quimérico resulta na alteração da estrutura fina do amido derivado do grão das ditas culturas de cereais transformadas, em comparação com a estrutura fina do amido derivado de culturas de cereais que não possuam o dito gene quimérico.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US9443698P | 1998-07-28 | 1998-07-28 | |
| PCT/US1999/016296 WO2000006755A2 (en) | 1998-07-28 | 1999-07-26 | Modification of starch biosynthetic enzyme gene expression to produce starches in grain crops |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR9912680A true BR9912680A (pt) | 2001-10-09 |
Family
ID=22245195
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR9912680-0A BR9912680A (pt) | 1998-07-28 | 1999-07-26 | Método de produção de uma cultura de ceral transformada, variedadde de milho, amido isolado do grão de uma variedade de milho, farinha preparada a partir de uma variedade de cultura de cereal, método de preparação de um composto alimentar espessado, variedade de milho transformada com um gene quimérico e amido |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US6392120B1 (pt) |
| EP (1) | EP1100938A2 (pt) |
| JP (1) | JP2002525029A (pt) |
| CN (1) | CN1314946A (pt) |
| AR (1) | AR020642A1 (pt) |
| AU (1) | AU765823B2 (pt) |
| BR (1) | BR9912680A (pt) |
| CA (1) | CA2330213C (pt) |
| HU (1) | HUP0104208A3 (pt) |
| RO (1) | RO120921B1 (pt) |
| WO (1) | WO2000006755A2 (pt) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7033627B2 (en) * | 1990-03-23 | 2006-04-25 | Syngenta Mogen B.V. | Production of enzymes in seeds and their use |
| US6392120B1 (en) | 1998-07-28 | 2002-05-21 | E. I. Du Pont De Nemours And Company | Modification of starch biosynthetic enzyme gene expression to produce starches in grain crops |
| AUPQ005299A0 (en) * | 1999-04-29 | 1999-05-27 | Commonwealth Scientific And Industrial Research Organisation | Novel genes encoding wheat starch synthases and uses therefor |
| EP1313866A1 (en) | 2000-08-28 | 2003-05-28 | E.I. Dupont de Nemours and Company | Novel starches produced by the expression of heterologous granule bound starch synthase genes |
| US7244839B2 (en) | 2002-03-27 | 2007-07-17 | E. I. Du Pont De Nemours And Company | Maize starch containing elevated amounts of actual amylose |
| AU2004252186B8 (en) | 2003-06-30 | 2010-06-24 | Commonwealth Scientific And Industrial Research Organisation | Wheat with altered branching enzyme activity and starch and starch containing products derived therefrom |
| CN1886507B (zh) | 2003-10-27 | 2010-12-29 | 联邦科技产业研究组织 | 淀粉中具有增加了直链淀粉含量的水稻及水稻制品 |
| GB0328154D0 (en) * | 2003-12-04 | 2004-01-07 | Plant Bioscience Ltd | Nucleic acids having utility in seeds |
| CN100402664C (zh) * | 2005-03-21 | 2008-07-16 | 中国科学院成都生物研究所 | 一种通过特异性dna片段识别水稻糯性基因的分子标记方法 |
| AR054174A1 (es) | 2005-07-22 | 2007-06-06 | Bayer Cropscience Gmbh | Sobreexpresion de sintasa de almidon en vegetales |
| US7674897B2 (en) * | 2005-09-09 | 2010-03-09 | Tate & Lyle Ingredients Americas, Inc. | Production of crystalline short chain amylose |
| AR064557A1 (es) * | 2006-12-29 | 2009-04-08 | Bayer Cropscience Ag | Almidon de maiz y harinas y alimentos de maiz que comprenden este almidon de maiz |
| WO2008125528A2 (en) * | 2007-04-13 | 2008-10-23 | Basf Plant Science Gmbh | Polynucleotides for regulation of high level tissue-preferred expression in crop plants |
| CN101665786B (zh) * | 2008-09-05 | 2012-07-18 | 中国科学院上海生命科学研究院 | 调节块根植物淀粉组成的方法 |
| EP2911519A4 (en) * | 2012-10-23 | 2016-06-01 | Univ Montana State | PRODUCTION OF HIGH QUALITY HARD WHEAT WITH INCREASED AMYLOSIS CONTENT |
| US9988641B2 (en) * | 2016-04-05 | 2018-06-05 | Corn Products Development, Inc. | Compositions and methods for producing starch with novel functionality |
| CN106337056B (zh) * | 2016-09-23 | 2018-10-02 | 中国热带农业科学院热带生物技术研究所 | 一种改良植物果实支链淀粉品质的基因及其编码产物与应用 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5300145B1 (en) * | 1992-08-28 | 1995-11-28 | Nat Starch Chem Invest | Low amylopectin starch |
| ES2217254T3 (es) | 1992-10-14 | 2004-11-01 | Syngenta Limited | Nuevas plantas y procesos para obtenerlas. |
| US5824790A (en) | 1994-06-21 | 1998-10-20 | Zeneca Limited | Modification of starch synthesis in plants |
| GB9524938D0 (en) | 1995-12-06 | 1996-02-07 | Zeneca Ltd | Modification of starch synthesis in plants |
| AU1684697A (en) | 1995-12-20 | 1997-07-14 | E.I. Du Pont De Nemours And Company | Novel starches via modification of expression of starch biosynthetic enzyme genes |
| DE19601365A1 (de) | 1996-01-16 | 1997-07-17 | Planttec Biotechnologie Gmbh | Nucleinsäuremoleküle aus Pflanzen codierend Enzyme, die an der Stärkesynthese beteiligt sind |
| DE19619918A1 (de) | 1996-05-17 | 1997-11-20 | Planttec Biotechnologie Gmbh | Nucleinsäuremoleküle codierend lösliche Stärkesynthasen aus Mais |
| CZ389098A3 (cs) | 1996-05-29 | 1999-02-17 | Hoechst Schering Agrevo Gmbh | Molekuly nukleové kyseliny kódující enzymy z pšenice účastnící se syntézy škrobu |
| JP2001505412A (ja) | 1996-09-30 | 2001-04-24 | エクシード・ジェネティックス・エル・エル・シー | ポリペプチドのデンプンマトリックスへの被包 |
| WO1998044780A1 (en) | 1997-04-04 | 1998-10-15 | Exseed Genetics, Llc | Plant like starches and the method of making them in hosts |
| US6392120B1 (en) | 1998-07-28 | 2002-05-21 | E. I. Du Pont De Nemours And Company | Modification of starch biosynthetic enzyme gene expression to produce starches in grain crops |
-
1999
- 1999-06-30 US US09/345,214 patent/US6392120B1/en not_active Expired - Lifetime
- 1999-07-26 JP JP2000562537A patent/JP2002525029A/ja active Pending
- 1999-07-26 WO PCT/US1999/016296 patent/WO2000006755A2/en active IP Right Grant
- 1999-07-26 CN CN99809049A patent/CN1314946A/zh active Pending
- 1999-07-26 AU AU52174/99A patent/AU765823B2/en not_active Ceased
- 1999-07-26 US US09/743,980 patent/US6570008B1/en not_active Expired - Lifetime
- 1999-07-26 HU HU0104208A patent/HUP0104208A3/hu unknown
- 1999-07-26 EP EP99937313A patent/EP1100938A2/en not_active Withdrawn
- 1999-07-26 CA CA2330213A patent/CA2330213C/en not_active Expired - Fee Related
- 1999-07-26 RO ROA200100080A patent/RO120921B1/ro unknown
- 1999-07-26 BR BR9912680-0A patent/BR9912680A/pt not_active IP Right Cessation
- 1999-07-28 AR ARP990103716A patent/AR020642A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US6570008B1 (en) | 2003-05-27 |
| CA2330213A1 (en) | 2000-02-10 |
| HUP0104208A2 (hu) | 2002-03-28 |
| AU5217499A (en) | 2000-02-21 |
| RO120921B1 (ro) | 2006-09-29 |
| CA2330213C (en) | 2012-01-03 |
| EP1100938A2 (en) | 2001-05-23 |
| CN1314946A (zh) | 2001-09-26 |
| WO2000006755A2 (en) | 2000-02-10 |
| WO2000006755A3 (en) | 2000-09-08 |
| HUP0104208A3 (en) | 2003-12-29 |
| AU765823B2 (en) | 2003-10-02 |
| US6392120B1 (en) | 2002-05-21 |
| JP2002525029A (ja) | 2002-08-13 |
| AR020642A1 (es) | 2002-05-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 4A, 5A, 6A, 7A E 8A ANUIDADES |
|
| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1911 DE 21/08/2007. |