HUP0104208A2 - Keményítő bioszintetikus enzim génexpressziójának megváltoztatása szemes terménynövényekben keményítő előállítása céljából - Google Patents

Keményítő bioszintetikus enzim génexpressziójának megváltoztatása szemes terménynövényekben keményítő előállítása céljából

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Publication number
HUP0104208A2
HUP0104208A2 HU0104208A HUP0104208A HUP0104208A2 HU P0104208 A2 HUP0104208 A2 HU P0104208A2 HU 0104208 A HU0104208 A HU 0104208A HU P0104208 A HUP0104208 A HU P0104208A HU P0104208 A2 HUP0104208 A2 HU P0104208A2
Authority
HU
Hungary
Prior art keywords
starch
cereal
chimeric gene
transformed
modification
Prior art date
Application number
HU0104208A
Other languages
English (en)
Inventor
Karen E. Broglie
Jonathan Edward Lightner
Original Assignee
E.I.Du Pont De Nemours And Co.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by E.I.Du Pont De Nemours And Co. filed Critical E.I.Du Pont De Nemours And Co.
Publication of HUP0104208A2 publication Critical patent/HUP0104208A2/hu
Publication of HUP0104208A3 publication Critical patent/HUP0104208A3/hu

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B30/00—Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Polymers & Plastics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Materials Engineering (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

lign=J;>(57) A találmány tárgyát képezi eljárás transzformált gabona-terménynövény előállítására, amely eljárás során (1) kiméra géntkészítenek, amely egy nem GBSSI keményítőszintáz-enzim szerkezetigénjét kódoló nukleinsavfragmenst vagy ennek egy fragmenséttartalmazza, és (2) a kiméra génnel gabona-terménynövényekettranszformálnak, ahol a kiméra gén expressziója a transzformáltgabona-terménynövények magjából származó keményítő finomszerkezetébenváltozást okoz összehasonlítva a kiméra gént nem tartalmazó gabona-terménynövénnyel. Az eljárás szerinti változás a keményítőfinomszerkezetében az amilóz-amilopektin relatív arányában, akeményítő amilopektinkomponense molekulatömeg-eloszlásában vagy akeményítő amilózkomponense polimerizációfokában nyilvánulhat meg. Atalálmány tárgyát képezik továbbá az eljárás alkalmazásávalelőállított gabonaterménynövény-változatok, a gabonaterménynövény-változatok magjából izolált keményítő és/vagy liszt, valamint eljárássűrített élelmiszerek előállítására. A találmány szerinti eljárássalelőállított keményítő élelmiszerként és ipari célokra alkalmazható. Ó
HU0104208A 1998-07-28 1999-07-26 Modification of starch biosynthetic enzyme gene expression to produce starches in grain crops HUP0104208A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9443698P 1998-07-28 1998-07-28
PCT/US1999/016296 WO2000006755A2 (en) 1998-07-28 1999-07-26 Modification of starch biosynthetic enzyme gene expression to produce starches in grain crops

Publications (2)

Publication Number Publication Date
HUP0104208A2 true HUP0104208A2 (hu) 2002-03-28
HUP0104208A3 HUP0104208A3 (en) 2003-12-29

Family

ID=22245195

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0104208A HUP0104208A3 (en) 1998-07-28 1999-07-26 Modification of starch biosynthetic enzyme gene expression to produce starches in grain crops

Country Status (11)

Country Link
US (2) US6392120B1 (hu)
EP (1) EP1100938A2 (hu)
JP (1) JP2002525029A (hu)
CN (1) CN1314946A (hu)
AR (1) AR020642A1 (hu)
AU (1) AU765823B2 (hu)
BR (1) BR9912680A (hu)
CA (1) CA2330213C (hu)
HU (1) HUP0104208A3 (hu)
RO (1) RO120921B1 (hu)
WO (1) WO2000006755A2 (hu)

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US7033627B2 (en) * 1990-03-23 2006-04-25 Syngenta Mogen B.V. Production of enzymes in seeds and their use
US6392120B1 (en) 1998-07-28 2002-05-21 E. I. Du Pont De Nemours And Company Modification of starch biosynthetic enzyme gene expression to produce starches in grain crops
AUPQ005299A0 (en) * 1999-04-29 1999-05-27 Commonwealth Scientific And Industrial Research Organisation Novel genes encoding wheat starch synthases and uses therefor
MXPA03001678A (es) * 2000-08-28 2003-09-10 Du Pont Nuevos almidones producidos mediante la expresion de genes de la sintasa de almidon enlazados a granulos heterologos.
US7244839B2 (en) 2002-03-27 2007-07-17 E. I. Du Pont De Nemours And Company Maize starch containing elevated amounts of actual amylose
WO2005001098A1 (en) 2003-06-30 2005-01-06 Commonwealth Scientific And Industrial Research Organisation Wheat with altered branching enzyme activity and starch and starch containing products derived thereform
AU2004284112B2 (en) 2003-10-27 2010-07-08 Commonwealth Scientific And Industrial Research Organisation Rice and products thereof having starch with an increased proportion of amylose
GB0328154D0 (en) * 2003-12-04 2004-01-07 Plant Bioscience Ltd Nucleic acids having utility in seeds
CN100402664C (zh) * 2005-03-21 2008-07-16 中国科学院成都生物研究所 一种通过特异性dna片段识别水稻糯性基因的分子标记方法
AR054174A1 (es) * 2005-07-22 2007-06-06 Bayer Cropscience Gmbh Sobreexpresion de sintasa de almidon en vegetales
US7674897B2 (en) * 2005-09-09 2010-03-09 Tate & Lyle Ingredients Americas, Inc. Production of crystalline short chain amylose
AR064557A1 (es) * 2006-12-29 2009-04-08 Bayer Cropscience Ag Almidon de maiz y harinas y alimentos de maiz que comprenden este almidon de maiz
EP2134845A2 (en) * 2007-04-13 2009-12-23 BASF Plant Science GmbH Polynucleotides for regulation of high level tissue-preferred expression in crop plants
CN101665786B (zh) * 2008-09-05 2012-07-18 中国科学院上海生命科学研究院 调节块根植物淀粉组成的方法
JP2015532124A (ja) 2012-10-23 2015-11-09 モンタナ ステート ユニバーシティ アミロース含量が増加した高品質デュラムコムギの生成
US9988641B2 (en) * 2016-04-05 2018-06-05 Corn Products Development, Inc. Compositions and methods for producing starch with novel functionality
CN106337056B (zh) * 2016-09-23 2018-10-02 中国热带农业科学院热带生物技术研究所 一种改良植物果实支链淀粉品质的基因及其编码产物与应用

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US5300145B1 (en) * 1992-08-28 1995-11-28 Nat Starch Chem Invest Low amylopectin starch
DE69233352T2 (de) 1992-10-14 2004-10-07 Syngenta Ltd Pflanzen und verfahren zu ihrer herstellung
US5824790A (en) 1994-06-21 1998-10-20 Zeneca Limited Modification of starch synthesis in plants
GB9524938D0 (en) 1995-12-06 1996-02-07 Zeneca Ltd Modification of starch synthesis in plants
HUP9902112A3 (en) 1995-12-20 2001-11-28 Du Pont Novel starches via modification of expression of starch biosynthetic enzyme genes
DE19601365A1 (de) 1996-01-16 1997-07-17 Planttec Biotechnologie Gmbh Nucleinsäuremoleküle aus Pflanzen codierend Enzyme, die an der Stärkesynthese beteiligt sind
DE19619918A1 (de) 1996-05-17 1997-11-20 Planttec Biotechnologie Gmbh Nucleinsäuremoleküle codierend lösliche Stärkesynthasen aus Mais
DE69737448T2 (de) 1996-05-29 2007-11-15 Bayer Cropscience Ag Nukleinsäuremoleküle, die für enzyme aus weizen kodieren, welche an der stärkesynthese beteiligt sind
IL129158A0 (en) 1996-09-30 2000-02-17 Exseed Genetics L L C Encapsulation of polypeptides within the starch matrix
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US6392120B1 (en) 1998-07-28 2002-05-21 E. I. Du Pont De Nemours And Company Modification of starch biosynthetic enzyme gene expression to produce starches in grain crops

Also Published As

Publication number Publication date
WO2000006755A2 (en) 2000-02-10
WO2000006755A3 (en) 2000-09-08
BR9912680A (pt) 2001-10-09
US6392120B1 (en) 2002-05-21
AU5217499A (en) 2000-02-21
AR020642A1 (es) 2002-05-22
HUP0104208A3 (en) 2003-12-29
US6570008B1 (en) 2003-05-27
EP1100938A2 (en) 2001-05-23
CA2330213A1 (en) 2000-02-10
AU765823B2 (en) 2003-10-02
CA2330213C (en) 2012-01-03
CN1314946A (zh) 2001-09-26
RO120921B1 (ro) 2006-09-29
JP2002525029A (ja) 2002-08-13

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