BR9808676B1 - construção genética, processos para produzir um polipeptìdeo epoxigenase enzimaticamente ativo, recombinante, e, polipeptìdeo recombinante de ocorrência não natural. - Google Patents

construção genética, processos para produzir um polipeptìdeo epoxigenase enzimaticamente ativo, recombinante, e, polipeptìdeo recombinante de ocorrência não natural.

Info

Publication number
BR9808676B1
BR9808676B1 BRPI9808676-6A BR9808676A BR9808676B1 BR 9808676 B1 BR9808676 B1 BR 9808676B1 BR 9808676 A BR9808676 A BR 9808676A BR 9808676 B1 BR9808676 B1 BR 9808676B1
Authority
BR
Brazil
Prior art keywords
epoxygenase
fatty acid
present
polypeptide
genetic sequences
Prior art date
Application number
BRPI9808676-6A
Other languages
English (en)
Other versions
BR9808676A (pt
Inventor
Sten Stymne
Allan Green
Surinder Singh
Marit Lenman
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AUPO6226A external-priority patent/AUPO622697A0/en
Priority claimed from AUPO6223A external-priority patent/AUPO622397A0/en
Application filed filed Critical
Publication of BR9808676A publication Critical patent/BR9808676A/pt
Publication of BR9808676B1 publication Critical patent/BR9808676B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Lubricants (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
BRPI9808676-6A 1997-04-15 1998-04-09 construção genética, processos para produzir um polipeptìdeo epoxigenase enzimaticamente ativo, recombinante, e, polipeptìdeo recombinante de ocorrência não natural. BR9808676B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
AUPO6226A AUPO622697A0 (en) 1997-04-15 1997-04-15 Plant fatty acid epoxygenase genes and uses therefor IIa
AUPO6223A AUPO622397A0 (en) 1997-04-15 1997-04-15 Plant fatty acid epoxygenase genes and uses therefor Ia
US4370697P 1997-04-16 1997-04-16
US5040397P 1997-06-20 1997-06-20
PCT/AU1998/000246 WO1998046762A1 (en) 1997-04-15 1998-04-09 Plant fatty acid epoxygenase genes and uses therefor

Publications (2)

Publication Number Publication Date
BR9808676A BR9808676A (pt) 2000-07-11
BR9808676B1 true BR9808676B1 (pt) 2010-10-05

Family

ID=27424435

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI9808676-6A BR9808676B1 (pt) 1997-04-15 1998-04-09 construção genética, processos para produzir um polipeptìdeo epoxigenase enzimaticamente ativo, recombinante, e, polipeptìdeo recombinante de ocorrência não natural.

Country Status (18)

Country Link
US (1) US6329518B1 (pt)
EP (1) EP0975765B1 (pt)
JP (1) JP4108765B2 (pt)
KR (1) KR100711074B1 (pt)
CN (1) CN100482797C (pt)
AT (1) ATE342361T1 (pt)
BR (1) BR9808676B1 (pt)
CA (1) CA2286895C (pt)
DE (1) DE69836133T2 (pt)
DK (1) DK0975765T3 (pt)
EA (1) EA004568B1 (pt)
ES (1) ES2275301T3 (pt)
IL (2) IL132393A0 (pt)
MX (1) MX224646B (pt)
NZ (1) NZ500542A (pt)
PL (1) PL194095B1 (pt)
TR (1) TR199902583T2 (pt)
WO (1) WO1998046762A1 (pt)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06240940A (ja) * 1993-02-22 1994-08-30 Takigen Seizo Kk 掛金装置
US6310194B1 (en) 1994-09-26 2001-10-30 Carnegie Institution Of Washington Plant fatty acid hydroxylases
US7589253B2 (en) * 1997-04-15 2009-09-15 Commonwealth Scientific And Industrial Research Organisation Fatty acid epoxygenase genes from plants and uses therefor in modifying fatty acid metabolism
IL139062A0 (en) * 1998-04-16 2001-11-25 Brookhaven Science Ass Llc Interconversion of plant fatty acid desaturases and hydroxylases
DE19941609A1 (de) * 1999-09-01 2001-03-08 Inst Pflanzenbiochemie Ipb Fettsäure-Desaturase-Gen aus Pflanzen
WO2001085968A2 (en) * 2000-05-09 2001-11-15 Bioriginal Food & Science Corp. Production of conjugated linoleic and linolenic acids in plants
CA2418155A1 (en) 2000-07-18 2002-01-24 National Research Council Of Canada Cloning, sequencing and expression of a comamonas cyclopentanone 1,2-monooxygenase-encoding gene in escherichia coli
PT1309698E (pt) * 2000-07-21 2007-06-22 Du Pont Uma enzima citocromo p450 associada com a síntese de grupos a12- epoxi em ácidos gordos de plantas.
US6485949B1 (en) * 2001-01-29 2002-11-26 The United States Of America As Represented By The Secretary Of Agriculture Epoxidation of carbon-carbon double bond with membrane bound peroxygenase
EP1578987A2 (en) * 2001-08-03 2005-09-28 Diversa Corporation Epoxide hydrolases, nucleic acids encoding them and methods for making and using them
US20040157803A1 (en) * 2002-03-04 2004-08-12 Williams Deryck J. Nematicidal fatty acid and fatty acid ester related compounds
US6887900B2 (en) 2002-03-04 2005-05-03 Divergence, Inc. Nematicidal compositions and methods
US7364901B2 (en) 2002-07-15 2008-04-29 University Of Kentucky Research Foundation Recombinant Stokesia epoxygenase gene
WO2004071168A2 (en) * 2003-02-05 2004-08-26 Divergence, Inc. Nucleic acids encoding anthelmintic agents and plants made therefrom
CN1310674C (zh) * 2003-04-30 2007-04-18 比奥滕普特公司 治疗重症急性呼吸综合征的药物组合物
WO2005014832A1 (en) * 2003-07-31 2005-02-17 The University Of York Fatty acid biosynthesis 4
AU2003255742A1 (en) * 2003-07-31 2005-02-25 The Universtiy Of York Fatty acid biosynthesis 1
WO2005014834A1 (en) * 2003-07-31 2005-02-17 The University Of York Fatty acid biosynthesis 3
US7368629B2 (en) * 2004-02-04 2008-05-06 Divergence, Inc. Nucleic acids encoding anthelmintic agents and plants made therefrom
CN101120091A (zh) * 2004-12-20 2008-02-06 巴斯福植物科学有限公司 编码植物脂肪酸去饱和酶基因的核酸分子及其使用方法
AU2006249983B2 (en) * 2005-05-23 2011-03-17 Arcadia Biosciences, Inc. Safflower with elevated gamma-linolenic acid
CA2722275A1 (en) * 2008-04-25 2009-10-29 Commonwealth Scientific And Industrial Research Organisation Polypeptides and methods for producing triacylglycerols comprising modified fatty acids
US8431772B1 (en) * 2008-11-19 2013-04-30 University Of Kentucky Research Foundation Diacylglycerol acyltransferase sequences and related methods
US9249082B2 (en) 2010-02-09 2016-02-02 King Abdulaziz City for Science and Technology (KACST) Synthesis of dimethyl carbonate from carbon dioxide and methanol
AU2012324024B2 (en) 2011-12-27 2016-06-16 Nuseed Global Innovation Ltd Processes for producing lipids
CA2933909A1 (en) 2013-12-18 2015-06-25 Grains Research And Development Corporation Lipid comprising long chain polyunsaturated fatty acids
CA2953008C (en) 2014-06-27 2024-03-19 Nuseed Pty Ltd Lipid comprising docosapentaenoic acid
BR112016030971B1 (pt) 2014-07-07 2022-09-27 Commonwealth Scientific And Industrial Research Organisation Processo para produzir um produto de óleo e processo para produzir um produto de óleo a partir do óleo da planta
US11913006B2 (en) 2018-03-16 2024-02-27 Nuseed Global Innovation Ltd. Plants producing modified levels of medium chain fatty acids
EP3543323A1 (de) * 2018-03-21 2019-09-25 Bergolin GmbH & Co. KG Verwendung eines insektenöls in harzen für beschichtungs- und klebstoffzusammensetzungen
CN116212948A (zh) * 2023-02-14 2023-06-06 四川九天五洋新材料有限责任公司 一种丙烯气相环氧化成型催化剂及其制备方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ227332A (en) 1987-12-15 1991-08-27 Gene Shears Pty Ltd Ribozymes, production and use
PH31293A (en) * 1991-10-10 1998-07-06 Rhone Poulenc Agrochimie Production of y-linolenic acid by a delta6-desaturage.
US5668292A (en) * 1994-09-26 1997-09-16 Carnegie Institution Of Washington Use of plant fatty acyl hydroxylases to produce hydroxylated fatty acids and derivatives in plants
US5834293A (en) * 1994-09-28 1998-11-10 Vanderbilt University Cytochrome P450 arachidonic acid epoxygenase genetic mutation associated with hypertension
SE9601236D0 (sv) 1996-03-29 1996-03-29 Sten Stymne Novel plant enzyme and use thereof

Also Published As

Publication number Publication date
IL132393A0 (en) 2001-03-19
US6329518B1 (en) 2001-12-11
WO1998046762A1 (en) 1998-10-22
DK0975765T3 (da) 2007-02-19
CN1257541A (zh) 2000-06-21
KR20010006468A (ko) 2001-01-26
EA004568B1 (ru) 2004-06-24
DE69836133T2 (de) 2007-08-16
CA2286895A1 (en) 1998-10-22
BR9808676A (pt) 2000-07-11
EP0975765B1 (en) 2006-10-11
ES2275301T3 (es) 2007-06-01
JP4108765B2 (ja) 2008-06-25
PL194095B1 (pl) 2007-04-30
EA199900933A1 (ru) 2000-04-24
CN100482797C (zh) 2009-04-29
PL336292A1 (en) 2000-06-19
CA2286895C (en) 2010-07-13
TR199902583T2 (xx) 2000-05-22
IL132393A (en) 2010-11-30
ATE342361T1 (de) 2006-11-15
EP0975765A4 (en) 2002-09-25
JP2001518797A (ja) 2001-10-16
NZ500542A (en) 2000-11-24
MX9909476A (es) 2000-04-30
MX224646B (es) 2004-12-03
EP0975765A1 (en) 2000-02-02
KR100711074B1 (ko) 2007-04-26
DE69836133D1 (de) 2006-11-23

Similar Documents

Publication Publication Date Title
BR9808676B1 (pt) construção genética, processos para produzir um polipeptìdeo epoxigenase enzimaticamente ativo, recombinante, e, polipeptìdeo recombinante de ocorrência não natural.
AR012399A1 (es) Una region regulatoria de 5' de oleosiina para la modificacion de composicion lipida de semilla de planta
BG103797A (en) Composition and method for polynonsaturated fatty acid syntheses with a long chain
DE69832283D1 (de) Herstellung von thioesterasen aus pflanzen und offenbarung von thioesterasen aus pflanzen mit neuer substratspezifität
DE69133283D1 (de) Dna, die für pflanzliche thioesterasen kodiert
AU552381B2 (en) Cocoabutter replacement fat composition
DE69334327D1 (de) Verfahren zur Herstellung sekretierter Proteine
AR056758A2 (es) Construccion de adn
WO2000066750A3 (en) Methods for producing plants with elevated oleic acid content
DE60027376D1 (de) Zusammensetzungen, enthaltend Pinolensäure sowie deren Verwendung zur Gesundheitserhaltung
CA2170761A1 (en) Lipid-Modified Serum Free Media
DE69935978D1 (de) Kulturmedium zur kultivierung tierischer zellen und verfahren zur herstellung von proteinen durch dessen verwendung
DE60024401D1 (de) Palmitoleinsäure und ihre Verwendung in Lebensmitteln
WO2001025453A3 (en) Plant fatty acid desaturases and alleles therefor
DK1122304T3 (da) Mikroorganisme der udskiller et lipid og fremgangsmåde til fremstilling af lipidet og lipidkugler med lipid indkapslet deri ved at anvende mikrororganismen
DE69837047D1 (de) Verfahren zur herstellung verzweigtkettiger fettsäuren mittels genetisch modifizierter pflanzen
BR9804026A (pt) Processo para produção de produtos da degradação de ácidos graxos.
JPS6410994A (en) Production of lower alcohol fatty acid ester
EP1637606A3 (de) Triacylglycerol-Lipasen
WO2003060092A3 (en) Modified fatty acid hydroxylase protein and genes
ATE192195T1 (de) Methode zur herstellung von peptidprodukten
DE69941849D1 (de) Herstellung eines biologischen faktors und schaffung einer immunologisch vorteilhaften umgebung unter verwendung genetisch veränderter sertolizellen
RU2002100130A (ru) $$$6-ацетиленаза и $$$6-десатураза из ceratodon purpureus
EP1624066A3 (en) Plant fatty acid synthases and use in improved methods for production of medium-chain fatty acids
AR016191A1 (es) Un procedimiento para incrementar el crecimiento y el rendimiento vegetal e incrementar la resistencia de una planta al estres y para modificar laproduccion de celulosa en una planta, dicha planta, un vector de adn de expresion y una semilla modificada geneticamente

Legal Events

Date Code Title Description
B25A Requested transfer of rights approved

Free format text: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION (AU), BASF PLANT SCIENCE GMBH (DE)

B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 05/10/2010, OBSERVADAS AS CONDICOES LEGAIS. COM RESSALVA NO QUE INCIDIR NO ARTIGO 10, INCISO IX, DA LPI 9279/96.

B24H Lapse because of non-payment of annual fees (definitively: art 78 iv lpi)
B24F Patent annual fee: publication cancelled

Free format text: ANULADA A PUBLICACAO CODIGO 24.8 NA RPI NO 2257 DE 08/04/2014 POR TER SIDO INDEVIDA.

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

Free format text: REFERENTE AS 13A, 14A, 15A, 16A, 17A, 18A, 19A, 20A, 21A E 22A ANUIDADES.

B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2606 DE 15-12-2020 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.