AR056758A2 - Construccion de adn - Google Patents

Construccion de adn

Info

Publication number
AR056758A2
AR056758A2 ARP060104795A ARP060104795A AR056758A2 AR 056758 A2 AR056758 A2 AR 056758A2 AR P060104795 A ARP060104795 A AR P060104795A AR P060104795 A ARP060104795 A AR P060104795A AR 056758 A2 AR056758 A2 AR 056758A2
Authority
AR
Argentina
Prior art keywords
acp
medium chain
expression
production
fatty acid
Prior art date
Application number
ARP060104795A
Other languages
English (en)
Original Assignee
Calgene Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Calgene Llc filed Critical Calgene Llc
Publication of AR056758A2 publication Critical patent/AR056758A2/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)

Abstract

Construccion de ADN que comprende una secuencia codificadora para una proteína KAS que demuestra actividad preferencial por sustratos de ácidos grasos de cadena media de C6:0-ACP, C8:0-ACP y C10:0-ACP. Tales construcciones son utiles para la produccion de plantas modificadas genéticamente que tienen composiciones de ácidos grasos alteradas. Es de especial interés la expresion de factores de proteína sintasa conjuntamente con la expresion de acil-ACP tioesterasas de cadena media vegetales para la produccion de niveles incrementados y/o relaciones modificadas de ácidos grasos de cadena media en aceites de semillas vegetales transgénicas.
ARP060104795A 1997-04-11 2006-11-01 Construccion de adn AR056758A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US4181597P 1997-04-11 1997-04-11

Publications (1)

Publication Number Publication Date
AR056758A2 true AR056758A2 (es) 2007-10-24

Family

ID=21918470

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP980101624A AR013633A1 (es) 1997-04-11 1998-04-08 METODO PARA LA ALTERACIoN DE LA COMPOSICIoN DE ÁCIDOS GRASOS DE CADENA MEDIA EN SEMILLAS VEGETALES QUE EXPRESAN UNA TIOESTERASA QUE PREFIERE CADENA MEDIA VEGETAL HETERoLOGA.
ARP060104795A AR056758A2 (es) 1997-04-11 2006-11-01 Construccion de adn

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ARP980101624A AR013633A1 (es) 1997-04-11 1998-04-08 METODO PARA LA ALTERACIoN DE LA COMPOSICIoN DE ÁCIDOS GRASOS DE CADENA MEDIA EN SEMILLAS VEGETALES QUE EXPRESAN UNA TIOESTERASA QUE PREFIERE CADENA MEDIA VEGETAL HETERoLOGA.

Country Status (12)

Country Link
US (3) US6660849B1 (es)
EP (1) EP1003890B1 (es)
JP (2) JP2002515761A (es)
KR (1) KR100515123B1 (es)
AR (2) AR013633A1 (es)
AT (1) ATE421592T1 (es)
AU (1) AU735445B2 (es)
BR (1) BR9808528A (es)
CA (1) CA2284286A1 (es)
DE (1) DE69840507D1 (es)
TW (1) TW567226B (es)
WO (1) WO1998046776A2 (es)

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Also Published As

Publication number Publication date
US20080148434A1 (en) 2008-06-19
TW567226B (en) 2003-12-21
ATE421592T1 (de) 2009-02-15
WO1998046776A9 (en) 2000-07-06
EP1003890A2 (en) 2000-05-31
US20040068765A1 (en) 2004-04-08
WO1998046776A3 (en) 2000-04-06
BR9808528A (pt) 2000-05-23
DE69840507D1 (de) 2009-03-12
AU735445B2 (en) 2001-07-05
KR20010006274A (ko) 2001-01-26
KR100515123B1 (ko) 2005-09-16
JP2002515761A (ja) 2002-05-28
AR013633A1 (es) 2001-01-10
AU6961198A (en) 1998-11-11
JP2009045074A (ja) 2009-03-05
US6660849B1 (en) 2003-12-09
CA2284286A1 (en) 1998-10-22
EP1003890B1 (en) 2009-01-21
WO1998046776A2 (en) 1998-10-22
US7301070B2 (en) 2007-11-27

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