EA199900933A1 - Гены эпоксигеназ жирных кислот растений и их использование - Google Patents

Гены эпоксигеназ жирных кислот растений и их использование

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Publication number
EA199900933A1
EA199900933A1 EA199900933A EA199900933A EA199900933A1 EA 199900933 A1 EA199900933 A1 EA 199900933A1 EA 199900933 A EA199900933 A EA 199900933A EA 199900933 A EA199900933 A EA 199900933A EA 199900933 A1 EA199900933 A1 EA 199900933A1
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EA
Eurasian Patent Office
Prior art keywords
fatty acid
present
genetic sequences
fatty acids
encode
Prior art date
Application number
EA199900933A
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English (en)
Other versions
EA004568B1 (ru
Inventor
Стен Стюмне
Аллан Грин
Суриндер Сингх
Марит Ленман
Original Assignee
Коммонвелт Сайентифик Энд Индастриал Рисерч Организейшн
Стен Стюмне
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AUPO6226A external-priority patent/AUPO622697A0/en
Priority claimed from AUPO6223A external-priority patent/AUPO622397A0/en
Application filed by Коммонвелт Сайентифик Энд Индастриал Рисерч Организейшн, Стен Стюмне filed Critical Коммонвелт Сайентифик Энд Индастриал Рисерч Организейшн
Publication of EA199900933A1 publication Critical patent/EA199900933A1/ru
Publication of EA004568B1 publication Critical patent/EA004568B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Lubricants (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

Настоящее изобретение касается главным образом новых генетических последовательностей, которые кодируют эпоксигеназные ферменты жирных кислот. В частности, настоящее изобретение касается генетических последовательностей, которые кодируют Δ12-эпоксигеназные ферменты жирных кислот, включающие монооксигеназные ферменты со смешанными функциями. Более предпочтительно, настоящее изобретение касается последовательностей кДНК, которые кодируют эпоксигеназы жирных кислот растений, в частности, Δ12-эпоксигеназу Crepis palaestina и ее гомологи, аналоги и производные. С помощью генетических последовательностей по настоящему изобретению можно изменять метаболизм жирных кислот или манипулировать этим метаболизмом в организмах, таких как дрожжи, плесневые грибы, бактерии, насекомые, птицы, млекопитающие и растения, в частности, преобразовывать в них ненасыщенные жирные кислоты в эпоксигенизированные жирные кислоты. Изобретение распространяется на генетически модифицированные накапливающие масла организмы, трансформированные генетическими последовательностями по настоящему изобретению, и на масла, получаемые от таких организмов. Вырабатываемые таким образом масла представляют собой экономически выгодный источник сырья для использования в эффективном производстве покрытий, смол, клеев, пластмасс, поверхностно-активных и смазочных веществ, среди прочего.Международная заявка была опубликована вместе с отчетом о международном поиске.
EA199900933A 1997-04-15 1998-04-09 Полипептиды монооксигеназы со смешанными функциями, способные катализировать эпоксигенизацию углеродной связи в молекуле жирной кислоты, кодирующие их нуклеиновые кислоты, способы применения полипептидов и нуклеиновых кислот и трансгенные растения EA004568B1 (ru)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
AUPO6226A AUPO622697A0 (en) 1997-04-15 1997-04-15 Plant fatty acid epoxygenase genes and uses therefor IIa
AUPO6223A AUPO622397A0 (en) 1997-04-15 1997-04-15 Plant fatty acid epoxygenase genes and uses therefor Ia
US4370697P 1997-04-16 1997-04-16
US5040397P 1997-06-20 1997-06-20
PCT/AU1998/000246 WO1998046762A1 (en) 1997-04-15 1998-04-09 Plant fatty acid epoxygenase genes and uses therefor

Publications (2)

Publication Number Publication Date
EA199900933A1 true EA199900933A1 (ru) 2000-04-24
EA004568B1 EA004568B1 (ru) 2004-06-24

Family

ID=27424435

Family Applications (1)

Application Number Title Priority Date Filing Date
EA199900933A EA004568B1 (ru) 1997-04-15 1998-04-09 Полипептиды монооксигеназы со смешанными функциями, способные катализировать эпоксигенизацию углеродной связи в молекуле жирной кислоты, кодирующие их нуклеиновые кислоты, способы применения полипептидов и нуклеиновых кислот и трансгенные растения

Country Status (18)

Country Link
US (1) US6329518B1 (ru)
EP (1) EP0975765B1 (ru)
JP (1) JP4108765B2 (ru)
KR (1) KR100711074B1 (ru)
CN (1) CN100482797C (ru)
AT (1) ATE342361T1 (ru)
BR (1) BR9808676B1 (ru)
CA (1) CA2286895C (ru)
DE (1) DE69836133T2 (ru)
DK (1) DK0975765T3 (ru)
EA (1) EA004568B1 (ru)
ES (1) ES2275301T3 (ru)
IL (2) IL132393A0 (ru)
MX (1) MX224646B (ru)
NZ (1) NZ500542A (ru)
PL (1) PL194095B1 (ru)
TR (1) TR199902583T2 (ru)
WO (1) WO1998046762A1 (ru)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06240940A (ja) * 1993-02-22 1994-08-30 Takigen Seizo Kk 掛金装置
US6310194B1 (en) 1994-09-26 2001-10-30 Carnegie Institution Of Washington Plant fatty acid hydroxylases
US7589253B2 (en) * 1997-04-15 2009-09-15 Commonwealth Scientific And Industrial Research Organisation Fatty acid epoxygenase genes from plants and uses therefor in modifying fatty acid metabolism
AU3862099A (en) * 1998-04-16 1999-11-01 Brookhaven Science Associates Llc Interconversion of plant fatty acid desaturases and hydroxylases
DE19941609A1 (de) * 1999-09-01 2001-03-08 Inst Pflanzenbiochemie Ipb Fettsäure-Desaturase-Gen aus Pflanzen
CA2408357A1 (en) * 2000-05-09 2001-11-15 Bioriginal Food & Science Corp. Production of conjugated linoleic and linolenic acids in plants
WO2002006452A2 (en) 2000-07-18 2002-01-24 National Research Council Of Canada Cloning, sequencing and expression of a comamonas cyclopentanone 1,2-monooxygenase-encoding gene in escherichia coli
JP2004513623A (ja) * 2000-07-21 2004-05-13 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 植物の脂肪酸におけるδ１２−エポキシ基の合成に関連するシトクロムｐ４５０酵素
US6485949B1 (en) * 2001-01-29 2002-11-26 The United States Of America As Represented By The Secretary Of Agriculture Epoxidation of carbon-carbon double bond with membrane bound peroxygenase
EP1578987A2 (en) * 2001-08-03 2005-09-28 Diversa Corporation Epoxide hydrolases, nucleic acids encoding them and methods for making and using them
US20040157803A1 (en) * 2002-03-04 2004-08-12 Williams Deryck J. Nematicidal fatty acid and fatty acid ester related compounds
US6887900B2 (en) 2002-03-04 2005-05-03 Divergence, Inc. Nematicidal compositions and methods
US7364901B2 (en) 2002-07-15 2008-04-29 University Of Kentucky Research Foundation Recombinant Stokesia epoxygenase gene
US7365240B2 (en) 2003-02-05 2008-04-29 Divergence, Inc. Nucleic acids encoding anthelmintic agents and plants made therefrom
CN1310674C (zh) * 2003-04-30 2007-04-18 比奥滕普特公司 治疗重症急性呼吸综合征的药物组合物
WO2005014834A1 (en) * 2003-07-31 2005-02-17 The University Of York Fatty acid biosynthesis 3
AU2003255742A1 (en) * 2003-07-31 2005-02-25 The Universtiy Of York Fatty acid biosynthesis 1
AU2003252954A1 (en) * 2003-07-31 2005-02-25 The University Of York Fatty acid biosynthesis 4
US7368629B2 (en) 2004-02-04 2008-05-06 Divergence, Inc. Nucleic acids encoding anthelmintic agents and plants made therefrom
EP1831380A2 (en) * 2004-12-20 2007-09-12 BASF Plant Science GmbH Nucleic acid molecules encoding fatty acid desaturase genes from plants and methods of use
AR053477A1 (es) * 2005-05-23 2007-05-09 Arcadia Biosciences Inc Cartamo con acido gamma - linolenico elevado
BRPI0911606A2 (pt) * 2008-04-25 2015-07-28 Commw Scient Ind Res Org Polipeptídeo e métodos para produzir triacilgliceróis compreendendo ácidos graxos modificados
US8431772B1 (en) * 2008-11-19 2013-04-30 University Of Kentucky Research Foundation Diacylglycerol acyltransferase sequences and related methods
US9249082B2 (en) 2010-02-09 2016-02-02 King Abdulaziz City for Science and Technology (KACST) Synthesis of dimethyl carbonate from carbon dioxide and methanol
MY196561A (en) 2011-12-27 2023-04-19 Commw Scient Ind Res Org Processes for Producing Lipids
CN104726473B (zh) 2013-12-18 2020-02-14 联邦科学技术研究组织 包含二十二碳六烯酸的提取的植物脂质
CN105219789B (zh) 2014-06-27 2023-04-07 联邦科学技术研究组织 包含二十二碳五烯酸的提取的植物脂质
EP4303288A3 (en) 2014-07-07 2024-03-06 Nuseed Global Innovation Ltd Processes for producing industrial products from plant lipids
US11913006B2 (en) 2018-03-16 2024-02-27 Nuseed Global Innovation Ltd. Plants producing modified levels of medium chain fatty acids
EP3543323A1 (de) * 2018-03-21 2019-09-25 Bergolin GmbH & Co. KG Verwendung eines insektenöls in harzen für beschichtungs- und klebstoffzusammensetzungen
CN116212948A (zh) * 2023-02-14 2023-06-06 四川九天五洋新材料有限责任公司 一种丙烯气相环氧化成型催化剂及其制备方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MC2115A1 (fr) 1987-12-15 1991-07-05 Gene Shears Pty Ltd Ribozynes
PH31293A (en) * 1991-10-10 1998-07-06 Rhone Poulenc Agrochimie Production of y-linolenic acid by a delta6-desaturage.
US5668292A (en) * 1994-09-26 1997-09-16 Carnegie Institution Of Washington Use of plant fatty acyl hydroxylases to produce hydroxylated fatty acids and derivatives in plants
US5834293A (en) * 1994-09-28 1998-11-10 Vanderbilt University Cytochrome P450 arachidonic acid epoxygenase genetic mutation associated with hypertension
SE9601236D0 (sv) 1996-03-29 1996-03-29 Sten Stymne Novel plant enzyme and use thereof

Also Published As

Publication number Publication date
BR9808676A (pt) 2000-07-11
DK0975765T3 (da) 2007-02-19
MX224646B (es) 2004-12-03
DE69836133D1 (de) 2006-11-23
KR20010006468A (ko) 2001-01-26
EP0975765B1 (en) 2006-10-11
CA2286895A1 (en) 1998-10-22
KR100711074B1 (ko) 2007-04-26
MX9909476A (es) 2000-04-30
NZ500542A (en) 2000-11-24
CN1257541A (zh) 2000-06-21
ATE342361T1 (de) 2006-11-15
EA004568B1 (ru) 2004-06-24
JP4108765B2 (ja) 2008-06-25
US6329518B1 (en) 2001-12-11
IL132393A (en) 2010-11-30
JP2001518797A (ja) 2001-10-16
EP0975765A4 (en) 2002-09-25
TR199902583T2 (xx) 2000-05-22
WO1998046762A1 (en) 1998-10-22
CN100482797C (zh) 2009-04-29
IL132393A0 (en) 2001-03-19
EP0975765A1 (en) 2000-02-02
DE69836133T2 (de) 2007-08-16
BR9808676B1 (pt) 2010-10-05
PL194095B1 (pl) 2007-04-30
CA2286895C (en) 2010-07-13
ES2275301T3 (es) 2007-06-01
PL336292A1 (en) 2000-06-19

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