BR9801130A - Fitases modificadas. - Google Patents

Fitases modificadas.

Info

Publication number
BR9801130A
BR9801130A BR9801130-8A BR9801130A BR9801130A BR 9801130 A BR9801130 A BR 9801130A BR 9801130 A BR9801130 A BR 9801130A BR 9801130 A BR9801130 A BR 9801130A
Authority
BR
Brazil
Prior art keywords
phytase
modified
dna sequence
host cell
active sites
Prior art date
Application number
BR9801130-8A
Other languages
English (en)
Other versions
BRPI9801130B1 (pt
Inventor
Dirk Kostrewa
Luis Pasamontes
Andrea Tomschy
Adolphus Van Loon
Kurt Vogel
Markus Wyss
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of BR9801130A publication Critical patent/BR9801130A/pt
Publication of BRPI9801130B1 publication Critical patent/BRPI9801130B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/065—Microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/913—Aspergillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/913—Aspergillus
    • Y10S435/916—Aspergillus fumigatus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/913—Aspergillus
    • Y10S435/917—Aspergillus niger

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Polymers & Plastics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

Patente de Invenção: <B>"FITASES MODIFICADAS"<D>. A presente invenção refere-se a um processo para a produção de uma fitase modificada com propriedades de atividades aperfeiçoadas, caracterizada pelo fato de que são efetuadas as seguintes etapas: a) a estrutura tridimensional da fitase a ser modificada e, opcionalmente, de outra fitase com propriedades de atividade que são mais favoráveis do que aquelas da fitase a ser modificada, é/são modelada(s) em computador com base na estrutura tridimensional da fitase de <MU>Aspergillus niger<MV>; b) a estrutura dos sítios ativos da fitase a ser modificada e da fitase com as propriedades de atividades mais favoráveis são comparadas e aqueles resíduos de aminoácidos em ambos os sítios ativos que sejam diferentes são identificados; c) uma seq³ência de ADN que codifica uma fitase modificada é construída modificando-se os nucleotídeos que codificam pelo menos um dos aminoácidos pelos quais ambos os sítios ativos se diferem; d) integrar uma tal seq³ência de ADN em um vetor capaz de expressão em uma célula de hospedeiro adequada; e) transformar uma célula de hospedeiro adequada pela seq³ência de ADN de c) ou o vetor d), crescer a dita célula de hospedeiro sob condições de crescimento adequadas e isolar a fitase modificada a partir da célula de hospedeiro ou do meio de cultura por processos conhecidos no estado da técnica. A presente invenção refere-se ainda à fitase modificada obtida ou obtenível por tal processo, à seq³ência de ADN que codifica esta fitase, ao vetor que compreende tal seq³ência de ADN, à célula de hospedeiro transformada por tal seq³ência de ADN e a um alimento ou composição alimentícia compreendendo esta fitase.
BRPI9801130A 1997-03-25 1998-03-25 "fitase modificada de aspergillus fumigatus com uma atividade específica aperfeiçoada e alimento ou composição alimentícia". BRPI9801130B1 (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP97810175 1997-03-25

Publications (2)

Publication Number Publication Date
BR9801130A true BR9801130A (pt) 2000-01-11
BRPI9801130B1 BRPI9801130B1 (pt) 2015-10-06

Family

ID=8230187

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI9801130A BRPI9801130B1 (pt) 1997-03-25 1998-03-25 "fitase modificada de aspergillus fumigatus com uma atividade específica aperfeiçoada e alimento ou composição alimentícia".

Country Status (10)

Country Link
US (3) US6391605B1 (pt)
EP (1) EP0897010B1 (pt)
JP (1) JP4097315B2 (pt)
KR (1) KR100498206B1 (pt)
CN (1) CN1146658C (pt)
AU (1) AU746874B2 (pt)
BR (1) BRPI9801130B1 (pt)
CA (1) CA2231948C (pt)
DE (1) DE69840617D1 (pt)
ES (1) ES2323819T3 (pt)

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CA2231948C (en) 1997-03-25 2010-05-18 F. Hoffmann-La Roche Ag Modified phytases
NZ330940A (en) 1997-07-24 2000-02-28 F Production of consensus phytases from fungal origin using computer programmes
EP1659173A1 (en) 1997-10-01 2006-05-24 DSMIP Assets B.V. Protein production process
ES2321047T3 (es) * 1998-03-23 2009-06-01 Novozymes A/S Variantes de fitasa.
AU2003270969B2 (en) * 1998-03-23 2006-08-03 Novozymes A/S Phytase Variants
US6514495B1 (en) 1998-03-23 2003-02-04 Novozymes A/S Phytase varinats
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US6610519B1 (en) 1998-10-02 2003-08-26 Novozymes A/S Solid phytase composition stabilized with lactic acid provided by corn steep liquor
US6720174B1 (en) 1999-01-28 2004-04-13 Novozymes A/S Phytases
CA2365418C (en) 1999-03-31 2009-05-26 Cornell Research Foundation, Inc. Phosphatases with improved phytase activity
US6303766B1 (en) 1999-05-14 2001-10-16 Virginia Tech Intellectual Properties, Inc. Soybean phytase and nucleic acid encoding the same
KR100351620B1 (ko) * 1999-07-09 2002-09-10 주식회사대성미생물연구소 3차 결정구조의 내열성 효소
BR0013549A (pt) 1999-08-13 2003-07-15 Univ Manchester Enzimas fitase, ácidos nucléicos codificando enzimas fitase e vetores e células hospedeiras incorporando as mesmas
EP1889904A3 (en) 1999-10-01 2008-07-09 Novozymes A/S Enzyme granulate
DE60043640D1 (de) 1999-10-11 2010-02-25 Dsm Ip Assets Bv Kontinuierliche Fermentation
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HUP0203259A2 (hu) * 1999-11-18 2003-02-28 Cornell Research Foundation, Inc. Az Escherichia Coli-ból származó fitáz helyspecifikus mutagenezise
AU2002356855A1 (en) 2001-10-26 2003-05-12 Genencor International, Inc. Phytase enzymes, nucleic acid sequences encoding phytase enzymes and vectors and host cells incorporating same
CN100475051C (zh) 2001-10-31 2009-04-08 菲特克斯有限责任公司 含有肌醇六磷酸酶的动物食物和方法
US7238378B2 (en) 2002-02-08 2007-07-03 Novozymes A/S Phytase variants
BRPI0307086B1 (pt) 2002-02-08 2015-12-15 Novozymes As variante de uma fitase parental, métodos para melhorar o valor nutritivo de uma ração animal, e para tratar proteínas vegetais, composição, processo para reduzir os níveis de fitato em um esterco animal, e, uso da variante
EP1604008B1 (en) 2002-09-13 2010-12-22 Cornell Research Foundation, Inc. Using mutations to improve aspergillus phytases
US20040063184A1 (en) 2002-09-26 2004-04-01 Novozymes North America, Inc. Fermentation processes and compositions
US20040253696A1 (en) 2003-06-10 2004-12-16 Novozymes North America, Inc. Fermentation processes and compositions
DE202005021811U1 (de) 2004-09-27 2010-04-15 Novozymes A/S Körnchen mit einem Kern und einer Beschichtung
US7587376B2 (en) * 2005-05-26 2009-09-08 International Business Machines Corporation Reformulation of constraint satisfaction problems for stochastic search
US7919297B2 (en) 2006-02-21 2011-04-05 Cornell Research Foundation, Inc. Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase
US7968318B2 (en) * 2006-06-06 2011-06-28 Genencor International, Inc. Process for conversion of granular starch to ethanol
US8540984B2 (en) 2006-08-03 2013-09-24 Cornell Research Foundation, Inc. Phytases with improved thermal stability
CN103168928A (zh) 2006-08-07 2013-06-26 诺维信公司 用于动物饲料的酶团粒
US9578891B2 (en) 2006-08-07 2017-02-28 Novozymes A/S Enzyme granules for animal feed
CN101144072B (zh) * 2006-09-13 2012-05-02 广东中大南海海洋生物技术工程中心有限公司 定点突变改造的植酸酶
US8192734B2 (en) 2007-07-09 2012-06-05 Cornell University Compositions and methods for bone strengthening
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CN103849607B (zh) * 2013-03-21 2016-04-20 成都国锦生物科技有限公司 耐高温植酸酶及其用途
EP3063282B1 (en) 2013-09-11 2020-03-25 Novozymes A/S Processes for producing fermentation products
WO2017112533A1 (en) 2015-12-22 2017-06-29 Novozymes A/S Process of extracting oil from thin stillage
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CN108251439B (zh) * 2018-01-11 2021-03-30 山西大学 一种人工改造的耐胰蛋白酶的植酸酶及其制备方法和应用
MX2020012754A (es) 2018-05-31 2021-02-26 Novozymes As Procesos para mejorar el crecimiento y la productividad de la levadura.
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Also Published As

Publication number Publication date
US20040142424A1 (en) 2004-07-22
AU746874B2 (en) 2002-05-02
DE69840617D1 (de) 2009-04-16
US20030092155A1 (en) 2003-05-15
KR19980080603A (ko) 1998-11-25
CA2231948C (en) 2010-05-18
EP0897010A2 (en) 1999-02-17
US6734004B2 (en) 2004-05-11
JPH10276789A (ja) 1998-10-20
EP0897010A3 (en) 1999-03-17
US7078183B2 (en) 2006-07-18
AU5954398A (en) 1998-10-01
JP4097315B2 (ja) 2008-06-11
CN1146658C (zh) 2004-04-21
EP0897010B1 (en) 2009-03-04
ES2323819T3 (es) 2009-07-24
KR100498206B1 (ko) 2005-09-09
US6391605B1 (en) 2002-05-21
CN1194305A (zh) 1998-09-30
BRPI9801130B1 (pt) 2015-10-06
CA2231948A1 (en) 1998-09-25

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Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 06/10/2015, OBSERVADAS AS CONDICOES LEGAIS.

B15K Others concerning applications: alteration of classification

Ipc: C12N 9/16 (2006.01), A23K 20/189 (2016.0

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

Free format text: REFERENTE A 23A ANUIDADE.

B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2622 DE 06-04-2021 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.