DE69840617D1 - Modifizierte Phytasen - Google Patents

Modifizierte Phytasen

Info

Publication number
DE69840617D1
DE69840617D1 DE69840617T DE69840617T DE69840617D1 DE 69840617 D1 DE69840617 D1 DE 69840617D1 DE 69840617 T DE69840617 T DE 69840617T DE 69840617 T DE69840617 T DE 69840617T DE 69840617 D1 DE69840617 D1 DE 69840617D1
Authority
DE
Germany
Prior art keywords
modified phytases
phytases
modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69840617T
Other languages
English (en)
Inventor
Dirk Kostrewa
Luis Pasamontes
Andrea Tomschy
Loon Adolphus Van
Kurt Vogel
Markus Wyss
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Application granted granted Critical
Publication of DE69840617D1 publication Critical patent/DE69840617D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/065—Microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/913—Aspergillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/913—Aspergillus
    • Y10S435/916—Aspergillus fumigatus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/913—Aspergillus
    • Y10S435/917—Aspergillus niger

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Polymers & Plastics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Fodder In General (AREA)
DE69840617T 1997-03-25 1998-03-18 Modifizierte Phytasen Expired - Lifetime DE69840617D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP97810175 1997-03-25

Publications (1)

Publication Number Publication Date
DE69840617D1 true DE69840617D1 (de) 2009-04-16

Family

ID=8230187

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69840617T Expired - Lifetime DE69840617D1 (de) 1997-03-25 1998-03-18 Modifizierte Phytasen

Country Status (10)

Country Link
US (3) US6391605B1 (de)
EP (1) EP0897010B1 (de)
JP (1) JP4097315B2 (de)
KR (1) KR100498206B1 (de)
CN (1) CN1146658C (de)
AU (1) AU746874B2 (de)
BR (1) BRPI9801130B1 (de)
CA (1) CA2231948C (de)
DE (1) DE69840617D1 (de)
ES (1) ES2323819T3 (de)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6699704B1 (en) 1994-04-25 2004-03-02 Roche Vitamins Inc. Heat tolerant phytases
CA2231948C (en) 1997-03-25 2010-05-18 F. Hoffmann-La Roche Ag Modified phytases
NZ330940A (en) * 1997-07-24 2000-02-28 F Production of consensus phytases from fungal origin using computer programmes
ES2264181T3 (es) 1997-10-01 2006-12-16 Dsm Ip Assets B.V. Procedimiento para la produccion de proteinas.
ES2321047T3 (es) * 1998-03-23 2009-06-01 Novozymes A/S Variantes de fitasa.
AU2003270969B2 (en) * 1998-03-23 2006-08-03 Novozymes A/S Phytase Variants
US6514495B1 (en) * 1998-03-23 2003-02-04 Novozymes A/S Phytase varinats
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
US6610519B1 (en) 1998-10-02 2003-08-26 Novozymes A/S Solid phytase composition stabilized with lactic acid provided by corn steep liquor
US6720174B1 (en) 1999-01-28 2004-04-13 Novozymes A/S Phytases
AU4056700A (en) 1999-03-31 2000-10-16 Cornell Research Foundation Inc. Phosphatases with improved phytase activity
US6303766B1 (en) 1999-05-14 2001-10-16 Virginia Tech Intellectual Properties, Inc. Soybean phytase and nucleic acid encoding the same
KR100351620B1 (ko) * 1999-07-09 2002-09-10 주식회사대성미생물연구소 3차 결정구조의 내열성 효소
US6475762B1 (en) * 1999-08-13 2002-11-05 Genencor International, Inc. Phytase enzymes nucleic acids encoding phytase enzymes and vectors and host cells incorporating same
WO2001025411A1 (en) 1999-10-01 2001-04-12 Novozymes A/S Spray dried enzyme product
ATE454442T1 (de) * 1999-10-11 2010-01-15 Dsm Ip Assets Bv Kontinuierliche fermentation
PL354805A1 (en) 1999-11-18 2004-02-23 Cornell Research Foundation, Inc. Site-directed mutagenesis of escherichia coli
US6841370B1 (en) 1999-11-18 2005-01-11 Cornell Research Foundation, Inc. Site-directed mutagenesis of Escherichia coli phytase
DK1438417T3 (da) 2001-10-26 2014-08-04 Danisco Us Inc Phytase-enzymer, nukleinsyresekvenser, der koder for fytase-enzymer ogvektorer og værtsceller, der inkorporerer disse
CA2465202C (en) 2001-10-31 2014-01-21 Phytex, Llc Phytase-containing animal food and method
EP1474508B1 (de) 2002-02-08 2011-11-16 Novozymes A/S Phytasenvarianten
US7238378B2 (en) 2002-02-08 2007-07-03 Novozymes A/S Phytase variants
ATE492631T1 (de) 2002-09-13 2011-01-15 Cornell Res Foundation Inc Verwendung von mutationen zur verbesserung von aspergillus-phytasen
US20040063184A1 (en) 2002-09-26 2004-04-01 Novozymes North America, Inc. Fermentation processes and compositions
US20040253696A1 (en) 2003-06-10 2004-12-16 Novozymes North America, Inc. Fermentation processes and compositions
EP2160950B1 (de) 2004-09-27 2015-05-13 Novozymes A/S Enzymgranulat
US7587376B2 (en) * 2005-05-26 2009-09-08 International Business Machines Corporation Reformulation of constraint satisfaction problems for stochastic search
US7919297B2 (en) 2006-02-21 2011-04-05 Cornell Research Foundation, Inc. Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase
US7968318B2 (en) 2006-06-06 2011-06-28 Genencor International, Inc. Process for conversion of granular starch to ethanol
WO2008017066A2 (en) 2006-08-03 2008-02-07 Cornell Research Foundation, Inc. Phytases with improved thermal stability
CN101500430B (zh) 2006-08-07 2014-02-19 诺维信公司 用于动物饲料的酶团粒
CN103168928A (zh) 2006-08-07 2013-06-26 诺维信公司 用于动物饲料的酶团粒
CN101144072B (zh) * 2006-09-13 2012-05-02 广东中大南海海洋生物技术工程中心有限公司 定点突变改造的植酸酶
US8192734B2 (en) 2007-07-09 2012-06-05 Cornell University Compositions and methods for bone strengthening
EP2116136A1 (de) 2008-05-08 2009-11-11 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO Neue Phytasen
CN103849607B (zh) * 2013-03-21 2016-04-20 成都国锦生物科技有限公司 耐高温植酸酶及其用途
EP3712274A1 (de) 2013-09-11 2020-09-23 Novozymes A/S Verfahren zur herstellung von gärungsprodukten
EP3394275A1 (de) 2015-12-22 2018-10-31 Novozymes A/S Verfahren zur extraktion von öl aus dünnsilage
CN111164214A (zh) 2017-09-15 2020-05-15 诺维信公司 用于改进动物饲料营养质量的酶共混物和方法
WO2019083831A1 (en) 2017-10-23 2019-05-02 Novozymes A/S METHODS FOR LACTIC ACID REDUCTION IN A BIOCARBURIZER FERMENTATION SYSTEM
CN108251439B (zh) * 2018-01-11 2021-03-30 山西大学 一种人工改造的耐胰蛋白酶的植酸酶及其制备方法和应用
CN112166197A (zh) 2018-05-31 2021-01-01 诺维信公司 用于增强酵母生长和生产力的方法
CN113226049A (zh) 2018-12-05 2021-08-06 诺维信公司 酶颗粒的用途
WO2020160126A1 (en) 2019-01-31 2020-08-06 Novozymes A/S Polypeptides having xylanase activity and use thereof for improving the nutritional quality of animal feed
WO2021026201A1 (en) 2019-08-05 2021-02-11 Novozymes A/S Enzyme blends and processes for producing a high protein feed ingredient from a whole stillage byproduct
MX2022006438A (es) 2019-12-16 2022-06-22 Novozymes As Procesos para obtener productos de fermentacion.
CN113862237B (zh) * 2021-12-02 2022-03-25 中国农业科学院北京畜牧兽医研究所 提高植酸酶的热稳定性的方法及突变体、基因和应用
CN115094049B (zh) * 2022-07-26 2024-05-28 青岛蔚蓝生物集团有限公司 耐高温中性植酸酶突变体
WO2024134574A1 (en) * 2022-12-23 2024-06-27 Basf Se Phytase variants with improved properties

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1492060C3 (de) 1965-09-08 1974-02-21 Nordmark-Werke Gmbh, 2000 Hamburg Verfahren zur Stabilisierung wäßriger Pepsinlösungen
EP0035204B2 (de) 1980-03-05 1991-05-22 Miles Inc. Pasteurisierte therapeutisch aktive Proteinzusammensetzungen
US6936694B1 (en) 1982-05-06 2005-08-30 Intermune, Inc. Manufacture and expression of large structural genes
US5780292A (en) 1987-04-29 1998-07-14 Alko Group Ltd. Production of phytate degrading enzymes in trichoderma
EP0299108B1 (de) 1987-07-17 1994-05-18 Rhein Biotech Gesellschaft für biotechnologische Prozesse und Produkte mbH DNA-Moleküle, die für FMDH-Kontrollabschnitte und Strukturgene für ein Protein mit FMDH-Aktivität kodieren, sowie deren Anwendung
CZ289014B6 (cs) 1989-09-27 2001-10-17 Dsm N. V. Vyčiątěná a izolovaná sekvence DNA kódující fungální fytázu, konstrukt pro expresi, vektory a transformované hostitelské buňky a způsob produkce fytázy
UA27702C2 (uk) 1989-09-27 2000-10-16 Гіст-Брокейдс Н.В. Фрагмент геномної днк, що кодує фітазу aspergillus niger,фрагмент кднк, що кодує фітазу aspergillus niger, рекомбінантна плазмідна днк для експресії фітази в aspergillus (варіанти), штам aspergillus-продуцент фітази aspergillus (варіанти), спосіб одержання фітази, рекомбінанта фітаза aspergillus niger
GB9006642D0 (en) 1990-03-24 1990-05-23 Gibson Timothy D Enzyme stabilisation
EP0626010A1 (de) 1992-02-13 1994-11-30 Gist-Brocades N.V. Stabilisierte, wässrige, flüssige, phytase enthaltende formulierungen
DE69333071T2 (de) 1993-04-05 2004-05-06 Aveve N.V. Phytate-Hydrolyse und enzymatische Zusammensetzung für die Hydrolyse von Phytat
DE705348T1 (de) 1993-06-21 2000-10-05 Roche Diagnostics Corp., Indianapolis Stabilisator für diagnostische reagentien
US6291221B1 (en) 1994-04-25 2001-09-18 Roche Vitamins Inc. Heat tolerant phytases
EP0684313B1 (de) * 1994-04-25 2006-07-05 DSM IP Assets B.V. Polypeptide mit phytase Wirkung
US6358722B1 (en) 1994-04-25 2002-03-19 Roche Vitamins, Inc. Heat tolerant phytases
EP0747483B1 (de) 1995-06-09 2004-03-24 DSM IP Assets B.V. Fermentative Herstellung von Carotenoiden
ES2220958T3 (es) 1995-07-28 2004-12-16 Basf Aktiengesellschaft Preparados enzimaticos estabilizados con una sal.
CA2231948C (en) 1997-03-25 2010-05-18 F. Hoffmann-La Roche Ag Modified phytases
ID24116A (id) 1997-06-04 2000-07-06 Dsm Nv Komposisi-komposisi fitase aktivitas tinggi
NZ330940A (en) 1997-07-24 2000-02-28 F Production of consensus phytases from fungal origin using computer programmes

Also Published As

Publication number Publication date
AU5954398A (en) 1998-10-01
US7078183B2 (en) 2006-07-18
KR100498206B1 (ko) 2005-09-09
BRPI9801130B1 (pt) 2015-10-06
CA2231948C (en) 2010-05-18
CN1194305A (zh) 1998-09-30
EP0897010A2 (de) 1999-02-17
EP0897010B1 (de) 2009-03-04
BR9801130A (pt) 2000-01-11
CA2231948A1 (en) 1998-09-25
EP0897010A3 (de) 1999-03-17
CN1146658C (zh) 2004-04-21
ES2323819T3 (es) 2009-07-24
JP4097315B2 (ja) 2008-06-11
KR19980080603A (ko) 1998-11-25
US6391605B1 (en) 2002-05-21
AU746874B2 (en) 2002-05-02
JPH10276789A (ja) 1998-10-20
US20030092155A1 (en) 2003-05-15
US6734004B2 (en) 2004-05-11
US20040142424A1 (en) 2004-07-22

Similar Documents

Publication Publication Date Title
DE69840617D1 (de) Modifizierte Phytasen
DE69826944D1 (de) Konsensus-Phytasen
DE19810476B4 (de) Laryngomaske
ATA93198A (de) Fluoxetinpellets
DE69841884D1 (de) Kabinenkommunikationsssystem
DE59804631D1 (de) Diketopyrrolopyrrolzusammensetzungen
DE59802506D1 (de) Weichspülmittelzusammemsetzung
DE59710388D1 (de) Mikropolarimeter
DE59803185D1 (de) Baulaser
DE59801498D1 (de) Dioxacycloalkan-8-one
DE59806387D1 (de) Azofarbstoffmischungen
DE59805979D1 (de) Organosilan-oligomere
ATA111897A (de) Kimme
ATE236897T1 (de) 3-benzylpiperidine
ATA112597A (de) Snowboard - bindung
DE59804377D1 (de) Kryoventil
DE59806025D1 (de) Normalsäschar
DE59805053D1 (de) Bogenflexodruckmaschine
DE59805424D1 (de) Transversalmodenresonatorfilter
ATA86797A (de) Verschleissschuh
DE59805451D1 (de) Coilboxofen
DE59800820D1 (de) Stiellose kniegelenkendoprothese
BR9701194A (pt) Hidrociclo
ATA46498A (de) Kunststoffgleitbahn
SE9704532D0 (sv) Kortställ

Legal Events

Date Code Title Description
8364 No opposition during term of opposition