HUP0203259A2 - Az Escherichia Coli-ból származó fitáz helyspecifikus mutagenezise - Google Patents

Az Escherichia Coli-ból származó fitáz helyspecifikus mutagenezise

Info

Publication number
HUP0203259A2
HUP0203259A2 HU0203259A HUP0203259A HUP0203259A2 HU P0203259 A2 HUP0203259 A2 HU P0203259A2 HU 0203259 A HU0203259 A HU 0203259A HU P0203259 A HUP0203259 A HU P0203259A HU P0203259 A2 HUP0203259 A2 HU P0203259A2
Authority
HU
Hungary
Prior art keywords
site
escherichia coli
directed mutagenesis
coli phytase
phytase
Prior art date
Application number
HU0203259A
Other languages
English (en)
Inventor
Xingen Lei
Original Assignee
Cornell Research Foundation, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cornell Research Foundation, Inc. filed Critical Cornell Research Foundation, Inc.
Publication of HUP0203259A2 publication Critical patent/HUP0203259A2/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03026—4-Phytase (3.1.3.26), i.e. 6-phytase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03008—3-Phytase (3.1.3.8)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Polymers & Plastics (AREA)
  • Animal Husbandry (AREA)
  • Food Science & Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)

Abstract

A jelen találmány tárgyát egy javított enzimatikus tulajdonságokkalrendelkező, izolált, mutáns savas foszfatáz/fitáz képezi. A mutánssavas foszfatáz/fitáz különösen alkalmas a takarmánykészítményekbentörténő felhasználásra. Ó
HU0203259A 1999-11-18 2000-11-17 Az Escherichia Coli-ból származó fitáz helyspecifikus mutagenezise HUP0203259A2 (hu)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16617999P 1999-11-18 1999-11-18
PCT/US2000/031622 WO2001036607A1 (en) 1999-11-18 2000-11-17 Site-directed mutagenesis of escherichia coli phytase

Publications (1)

Publication Number Publication Date
HUP0203259A2 true HUP0203259A2 (hu) 2003-02-28

Family

ID=22602133

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0203259A HUP0203259A2 (hu) 1999-11-18 2000-11-17 Az Escherichia Coli-ból származó fitáz helyspecifikus mutagenezise

Country Status (11)

Country Link
EP (1) EP1230350B1 (hu)
JP (1) JP2003531574A (hu)
KR (1) KR100790918B1 (hu)
CN (1) CN1423694A (hu)
AU (1) AU1618801A (hu)
CA (1) CA2391739C (hu)
CZ (1) CZ20021701A3 (hu)
HU (1) HUP0203259A2 (hu)
MX (1) MXPA02004989A (hu)
PL (1) PL354805A1 (hu)
WO (1) WO2001036607A1 (hu)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
AU2002356880A1 (en) 2001-10-31 2003-05-12 Phytex, Llc Phytase-containing animal food and method
SE0200911D0 (sv) 2002-03-22 2002-03-22 Chalmers Technology Licensing Phytase active yeast
US7968342B2 (en) 2002-08-12 2011-06-28 Danisco Us Inc. Mutant E. coli appa phytase enzymes and natural variants thereof, nucleic acids encoding such phytase enzymes, vectors and host cells incorporating same and methods of making and using same
CA2498017C (en) 2002-09-13 2016-06-14 Cornell Research Foundation, Inc. Using mutations to improve aspergillus phytases
US7276362B2 (en) 2004-01-30 2007-10-02 Roche Diagnostics Operations, Inc. Recombinant histidine-tagged inosine monophosphate dehydrogenase polypeptides
DE102004050410A1 (de) 2004-10-15 2006-06-08 Ab Enzymes Gmbh Polypeptid mit Phytaseaktivität und dieses codierende Nucleotidsequenz
US7919297B2 (en) 2006-02-21 2011-04-05 Cornell Research Foundation, Inc. Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase
ES2531434T3 (es) * 2006-04-04 2015-03-16 Novozymes A/S Variantes de fitasa
WO2008017066A2 (en) 2006-08-03 2008-02-07 Cornell Research Foundation, Inc. Phytases with improved thermal stability
EP2617814B1 (en) * 2006-09-21 2015-11-18 BASF Enzymes LLC Phytases, nucleic acids encoding them and methods for making and using them
DE102006053059A1 (de) 2006-11-10 2008-05-15 Ab Enzymes Gmbh Polypeptid mit Phytaseaktivität und erhöhter Temperaturstabilität der Enzymaktivität sowie dieses codierende Nukleotidsequenz
US8192734B2 (en) 2007-07-09 2012-06-05 Cornell University Compositions and methods for bone strengthening
AU2010249500B2 (en) 2009-05-21 2016-03-24 Basf Enzymes Llc Phytases, nucleic acids encoding them and methods for making and using them
CN102102094B (zh) * 2009-12-16 2013-03-27 福建福大百特科技发展有限公司 热稳定脂肪酶,其编码基因的表达及其用途
CN104487571A (zh) * 2012-02-07 2015-04-01 丹尼斯科美国公司 糖基化作为植酸酶的稳定剂
WO2015070372A1 (zh) * 2013-11-12 2015-05-21 中国农业科学院饲料研究所 一种产生热稳定性改良的植酸酶变体的方法、以及植酸酶变体及其应用
KR101695397B1 (ko) * 2016-02-29 2017-01-13 주식회사 비즈모델라인 가맹점의 고유코드를 이용한 결제 방법
CN107353327A (zh) * 2017-03-30 2017-11-17 南京百斯杰生物工程有限公司 植酸酶在黑曲霉中表达
EP3453719A1 (en) 2017-09-07 2019-03-13 Huvepharma Eood New thermostable phytases with high catalytic efficacy
CN110484520B (zh) * 2018-05-14 2023-08-04 广东溢多利生物科技股份有限公司 比活提高的植酸酶appa突变体及其基因和应用
CN111218436B (zh) * 2018-11-27 2022-08-30 青岛蔚蓝生物集团有限公司 一种植酸酶突变体
US20230034069A1 (en) * 2019-12-23 2023-02-02 Cargill, Incorporated Fermentation method and uses thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2231948C (en) * 1997-03-25 2010-05-18 F. Hoffmann-La Roche Ag Modified phytases

Also Published As

Publication number Publication date
PL354805A1 (en) 2004-02-23
KR100790918B1 (ko) 2008-01-03
EP1230350B1 (en) 2014-01-22
WO2001036607A1 (en) 2001-05-25
CA2391739A1 (en) 2001-05-25
AU1618801A (en) 2001-05-30
CA2391739C (en) 2013-04-09
JP2003531574A (ja) 2003-10-28
KR20020061619A (ko) 2002-07-24
EP1230350A1 (en) 2002-08-14
EP1230350A4 (en) 2003-02-19
CZ20021701A3 (cs) 2003-06-18
MXPA02004989A (es) 2003-01-28
CN1423694A (zh) 2003-06-11

Similar Documents

Publication Publication Date Title
HUP0203259A2 (hu) Az Escherichia Coli-ból származó fitáz helyspecifikus mutagenezise
AU2002347019A1 (en) Polypeptides having protease activity and nucleic acids encoding same
HUP0202759A2 (hu) Táptalaj sejtek fehérjementes és szérummentes tenyésztéséhez
WO2001051621A3 (en) Dna polymerase mutant having one or more mutations in the active site
AU5242000A (en) Nematicidal trifluorobutenes
AU2003302626A1 (en) 1, 2, 5-thiadiazolidin-3-one 1, 1 dioxide derivatives as inhibitors of protein tyrosine phosphatase ptp1b
AU2001237425A1 (en) Rna polymerase mutants with increased thermostability
NO20003482L (no) Fremgangsmåte for utvikling av vitalt hvetegluten i ikke- vandige media
HU9601087D0 (en) Arabinoxylane degrading enzymes
AU5186799A (en) Peptides of il-2 and derivatives thereof and their use as therapeutic agents
IL129055A (en) Mutant penicillin g acylases
AU2003272410A1 (en) Using mutations to improve aspergillus phytases
SE9604251D0 (sv) Födoämne med ny funktion
NO20051148L (no) Formulering for nyfodte inneholdene delvis hydrolysert isolert soya protein med redusert fytatinnhold
RS20050294A (en) Novel pyrimidine-4,6-dicarboxamides for the selective inhibition of collagenases
AU2002220697A1 (en) Isolated luciferases lu164, lual and lu22, and the use of the same
WO2003070229A3 (de) Verwendung von proteinaseinhibitoren zur behandlung von autoimmunerkrankungen
WO2003029469A1 (fr) Nouvelles proteines contenant de la selenocysteine
SI1276495T1 (sl) Uporaba rekombinantnega pljucnega surfaktanta za zgodnje zdravljenje akutnih pljucnih bolezni
AU2003287945A1 (en) Mutant protein and refolding method
ES2156812A1 (es) Una proteasa extracelular de acremonium chrysogenum con actividad cpc-acetilhidrolasa y su utilizacion para la sintesis de derivados desacetilados de cefalosporina c e inactivacion del gen para aumentar la produccion de cefalosporina.
AU8011498A (en) Novel ppgpp synthetase and expression systems for improved production of proteinof interest
DE60004362D1 (de) Thiazolopyrimidine verwendbar als TNF-Alpha Inhibitoren
DE69938427D1 (de) Tes gen und dessen verwendung
AU6601000A (en) 3-oxopropane-1-sulphonic acids and sulphonates