BR112014012910B1 - Método para produção de uma mistura de enzimas que catalisam um processo de conversão e método para preparação de um banco de células - Google Patents

Método para produção de uma mistura de enzimas que catalisam um processo de conversão e método para preparação de um banco de células Download PDF

Info

Publication number
BR112014012910B1
BR112014012910B1 BR112014012910-0A BR112014012910A BR112014012910B1 BR 112014012910 B1 BR112014012910 B1 BR 112014012910B1 BR 112014012910 A BR112014012910 A BR 112014012910A BR 112014012910 B1 BR112014012910 B1 BR 112014012910B1
Authority
BR
Brazil
Prior art keywords
enzymes
cell lines
cell
cell line
enzyme
Prior art date
Application number
BR112014012910-0A
Other languages
English (en)
Portuguese (pt)
Other versions
BR112014012910A2 (pt
Inventor
Suzanne E. Lantz
George England
Original Assignee
Danisco Us Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc. filed Critical Danisco Us Inc.
Publication of BR112014012910A2 publication Critical patent/BR112014012910A2/pt
Publication of BR112014012910B1 publication Critical patent/BR112014012910B1/pt

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
BR112014012910-0A 2011-12-13 2012-12-04 Método para produção de uma mistura de enzimas que catalisam um processo de conversão e método para preparação de um banco de células BR112014012910B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161570243P 2011-12-13 2011-12-13
US61/570,243 2011-12-13
PCT/US2012/067717 WO2013090053A1 (en) 2011-12-13 2012-12-04 Enzyme cocktails prepared from mixed cultures

Publications (2)

Publication Number Publication Date
BR112014012910A2 BR112014012910A2 (pt) 2020-10-20
BR112014012910B1 true BR112014012910B1 (pt) 2021-06-29

Family

ID=47352051

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014012910-0A BR112014012910B1 (pt) 2011-12-13 2012-12-04 Método para produção de uma mistura de enzimas que catalisam um processo de conversão e método para preparação de um banco de células

Country Status (14)

Country Link
US (1) US20140295475A1 (https=)
EP (1) EP2791350B1 (https=)
JP (1) JP2015500041A (https=)
KR (1) KR20140122704A (https=)
CN (1) CN104011222A (https=)
AU (1) AU2012352763A1 (https=)
BR (1) BR112014012910B1 (https=)
CA (1) CA2858373A1 (https=)
DK (1) DK2791350T3 (https=)
HK (1) HK1203083A1 (https=)
MX (1) MX367078B (https=)
PH (1) PH12014500771A1 (https=)
RU (1) RU2014128577A (https=)
WO (1) WO2013090053A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2480660T5 (da) 2009-09-23 2020-11-09 Danisco Us Inc Hidtil ukendte glycosylhydrolaseenzymer og anvendelser heraf
AU2010333801B2 (en) 2009-12-23 2015-12-17 Danisco Us Inc. Methods for improving the efficiency of simultaneous saccharification and fermentation reactions
JP6290626B2 (ja) 2011-03-17 2018-03-14 ダニスコ・ユーエス・インク 糖化プロセスにおける粘度を低下させる方法
US20170183691A1 (en) * 2014-04-21 2017-06-29 Danisco Us Inc. Simultaneous saccharification and co-fermentation of glucoamylase-expressing fungal strains with an ethanologen to produce alcohol from corn
CN105695439B (zh) * 2014-11-27 2023-08-01 丰益(上海)生物技术研发中心有限公司 一种β-葡萄糖苷酶基因的重组表达方法
CN108026559A (zh) * 2015-10-21 2018-05-11 诺维信公司 直接接种
WO2018106656A1 (en) 2016-12-06 2018-06-14 Danisco Us Inc Truncated lpmo enzymes and use thereof
JP7316758B2 (ja) * 2018-03-02 2023-07-28 合同酒精株式会社 オフフレーバーのリスクを低減できるラクターゼ調製物
CN109852557B (zh) * 2018-11-30 2021-03-26 北京国环清华环境工程设计研究院有限公司 一种用于城镇存量垃圾稳定化处理的复合菌剂及其制备方法与应用
US12416026B2 (en) 2019-08-29 2025-09-16 Danisco Us Inc. Expression of beta-glucosidase in yeast for improved ethanol production
US11597950B1 (en) 2020-03-11 2023-03-07 Poet Research, Inc. Systems, compositions, and methods of fermentation with Z. mobilis
US11732278B1 (en) 2020-03-11 2023-08-22 Poet Research, Inc. Systems and methods for co-culture of oxygen sensitive bacteria and yeast

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5182204A (en) 1984-05-29 1993-01-26 Genencor International, Inc. Non-human carbonyl hydrolase mutants, vectors encoding same and hosts transformed with said vectors
FR2625295B1 (fr) 1987-12-24 1990-04-13 Gaz De France Procede et appareil destines a assurer la combustion etagee d'un melange combustible-comburant diminuant la production d'oxydes d'azote
US5958739A (en) 1996-06-06 1999-09-28 Genencor International Inc. Mutant α-amylase
AR016969A1 (es) 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
US20030180900A1 (en) * 2002-02-08 2003-09-25 Oreste Lantero Methods for producing ethanol from carbon substrates
ATE524491T1 (de) 2003-05-29 2011-09-15 Genencor Int Neue trichoderma-gene
WO2004113490A2 (en) * 2003-06-24 2004-12-29 HöFer Bioreact GmbH Directed selective solid-phase culturing of stable microbial mixed populations for the continuous preparation of defined enzyme and metabolite mixture and bioreactor
US20090246844A1 (en) * 2007-10-26 2009-10-01 Arbor Fuel Inc. Methods for the production of ethanol
CN101918572A (zh) * 2007-10-26 2010-12-15 阿伯燃料公司 正丁醇的生产方法
US8652819B2 (en) * 2008-05-23 2014-02-18 University Of Georgia Research Foundation, Inc. Paenibacillus spp. and methods for fermentation of lignocellulosic materials
CN102057040A (zh) 2008-06-06 2011-05-11 丹尼斯科美国公司 具有改良特性的嗜热脂肪土芽孢杆菌α-淀粉酶(AMYS)变体
CA2744495C (en) * 2008-11-21 2017-05-30 Mascoma Corporation Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose
WO2010141779A1 (en) 2009-06-03 2010-12-09 Danisco Us Inc. Cellulase variants with improved expression, activity and/or stability, and use thereof
DK2292731T3 (en) 2009-08-13 2018-07-23 Dupont Nutrition Biosci Aps Process for producing complex cultures

Also Published As

Publication number Publication date
US20140295475A1 (en) 2014-10-02
CA2858373A1 (en) 2013-06-20
CN104011222A (zh) 2014-08-27
KR20140122704A (ko) 2014-10-20
DK2791350T3 (da) 2019-05-06
AU2012352763A1 (en) 2014-04-24
MX367078B (es) 2019-08-05
WO2013090053A1 (en) 2013-06-20
MX2014006570A (es) 2014-08-21
HK1203083A1 (en) 2015-10-16
BR112014012910A2 (pt) 2020-10-20
EP2791350A1 (en) 2014-10-22
EP2791350B1 (en) 2019-01-23
JP2015500041A (ja) 2015-01-05
RU2014128577A (ru) 2016-02-10
PH12014500771A1 (en) 2021-08-09

Similar Documents

Publication Publication Date Title
BR112014012910B1 (pt) Método para produção de uma mistura de enzimas que catalisam um processo de conversão e método para preparação de um banco de células
Manavalan et al. Secretome analysis of Ganoderma lucidum cultivated in sugarcane bagasse
CA2657684C (en) Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US8580536B2 (en) Compositions for saccharification of cellulosic material
Chen et al. Isolation and characterization of a β-glucosidase from Penicillium decumbens and improving hydrolysis of corncob residue by using it as cellulase supplementation
JP5785976B2 (ja) ベータ‐グルコシダーゼ強化糸状菌全セルラーゼ組成物及び使用方法
KR20140017612A (ko) 당화 공정에서 점도를 감소시키는 방법
KR20120106774A (ko) 동시 당화 발효 반응의 효율을 향상시키는 방법
Ueda et al. Cloning and expression of the cold-adapted endo-1, 4-β-glucanase gene from Eisenia fetida
KR20140027154A (ko) 글리코실 가수분해효소 및 바이오매스 가수분해를 위한 그의 용도
CN103890168A (zh) 用于蛋白质分泌和木质纤维素降解的突变细胞
CN111094562A (zh) 具有海藻糖酶活性的多肽及其在产生发酵产物的方法中的用途
CN110997701A (zh) 具有海藻糖酶活性的多肽以及编码其的多核苷酸
Gao et al. The production of β-glucosidases by Fusarium proliferatum NBRC109045 isolated from Vietnamese forest
CN103562402A (zh) 热稳定性木霉属纤维素酶
Raheja et al. Combination of system biology and classical approaches for developing biorefinery relevant lignocellulolytic Rasamsonia emersonii strain
Sinitsyn et al. The production of highly effective enzyme complexes of cellulases and hemicellulases based on the Penicillium verruculosum strain for the hydrolysis of plant raw materials
KR20120013958A (ko) 발효 조건을 통한 효소 균형 변경
Semenova et al. Isolation and properties of extracellular β-xylosidases from fungi Aspergillus japonicus and Trichoderma reesei
US20170183691A1 (en) Simultaneous saccharification and co-fermentation of glucoamylase-expressing fungal strains with an ethanologen to produce alcohol from corn
CN103562216A (zh) 纤维糊精和β-D-葡萄糖的增强型发酵
CN112771069B (zh) 表达外源葡糖淀粉酶和木聚糖酶的酿酒酵母菌株及其在生产生物乙醇中的用途
Imangholiloo et al. Expressed cellobiohydrolase enzyme of thermobifidia fusca in Pichia pastoris as host can act on cotton substrate
CN105950591A (zh) 一种中性低温木聚糖酶CaXyn10A及其基因和应用
EP4638768A2 (en) Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast

Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 04/12/2012, OBSERVADAS AS CONDICOES LEGAIS.

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

Free format text: REFERENTE A 12A ANUIDADE.

B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2804 DE 01-10-2024 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.