AU2012352763A1 - Enzyme cocktails prepared from mixed cultures - Google Patents
Enzyme cocktails prepared from mixed cultures Download PDFInfo
- Publication number
- AU2012352763A1 AU2012352763A1 AU2012352763A AU2012352763A AU2012352763A1 AU 2012352763 A1 AU2012352763 A1 AU 2012352763A1 AU 2012352763 A AU2012352763 A AU 2012352763A AU 2012352763 A AU2012352763 A AU 2012352763A AU 2012352763 A1 AU2012352763 A1 AU 2012352763A1
- Authority
- AU
- Australia
- Prior art keywords
- enzymes
- cell lines
- cell
- cell line
- different
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 360
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 360
- 238000000034 method Methods 0.000 claims abstract description 204
- 239000000203 mixture Substances 0.000 claims abstract description 71
- 241000499912 Trichoderma reesei Species 0.000 claims description 66
- 238000006243 chemical reaction Methods 0.000 claims description 66
- 230000012010 growth Effects 0.000 claims description 64
- 230000008569 process Effects 0.000 claims description 54
- 230000002538 fungal effect Effects 0.000 claims description 34
- 241000894007 species Species 0.000 claims description 34
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 26
- 239000000758 substrate Substances 0.000 claims description 25
- 239000008103 glucose Substances 0.000 claims description 23
- 239000007788 liquid Substances 0.000 claims description 23
- 229920002472 Starch Polymers 0.000 claims description 21
- 235000019698 starch Nutrition 0.000 claims description 20
- 239000008107 starch Substances 0.000 claims description 19
- 238000012258 culturing Methods 0.000 claims description 16
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 14
- 230000001580 bacterial effect Effects 0.000 claims description 13
- 229920002678 cellulose Polymers 0.000 claims description 13
- 230000000694 effects Effects 0.000 claims description 13
- 239000001913 cellulose Substances 0.000 claims description 12
- 229920002488 Hemicellulose Polymers 0.000 claims description 11
- 230000002708 enhancing effect Effects 0.000 claims description 11
- 235000000346 sugar Nutrition 0.000 claims description 8
- 230000000813 microbial effect Effects 0.000 claims description 6
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims description 4
- 230000003197 catalytic effect Effects 0.000 claims description 3
- 230000001902 propagating effect Effects 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 362
- 229940088598 enzyme Drugs 0.000 description 302
- 108090000623 proteins and genes Proteins 0.000 description 55
- 239000000047 product Substances 0.000 description 49
- 239000013615 primer Substances 0.000 description 41
- 230000014509 gene expression Effects 0.000 description 38
- 102000004169 proteins and genes Human genes 0.000 description 36
- 238000000855 fermentation Methods 0.000 description 31
- 238000004519 manufacturing process Methods 0.000 description 27
- 230000002441 reversible effect Effects 0.000 description 27
- 239000004382 Amylase Substances 0.000 description 26
- 108010065511 Amylases Proteins 0.000 description 26
- 102000013142 Amylases Human genes 0.000 description 26
- 235000019418 amylase Nutrition 0.000 description 25
- 238000003501 co-culture Methods 0.000 description 25
- 241000233866 Fungi Species 0.000 description 24
- DRTQHJPVMGBUCF-XVFCMESISA-N Uridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-XVFCMESISA-N 0.000 description 24
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 23
- 230000004151 fermentation Effects 0.000 description 23
- 238000004128 high performance liquid chromatography Methods 0.000 description 23
- 108010059892 Cellulase Proteins 0.000 description 21
- 239000001963 growth medium Substances 0.000 description 21
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 20
- 239000002609 medium Substances 0.000 description 20
- 108020004414 DNA Proteins 0.000 description 19
- 102100022624 Glucoamylase Human genes 0.000 description 19
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 18
- 239000013598 vector Substances 0.000 description 16
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 15
- 229940025131 amylases Drugs 0.000 description 15
- 230000004927 fusion Effects 0.000 description 15
- 241000894006 Bacteria Species 0.000 description 14
- 235000010633 broth Nutrition 0.000 description 14
- 239000012634 fragment Substances 0.000 description 13
- 239000002585 base Substances 0.000 description 12
- DRTQHJPVMGBUCF-PSQAKQOGSA-N beta-L-uridine Natural products O[C@H]1[C@@H](O)[C@H](CO)O[C@@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-PSQAKQOGSA-N 0.000 description 12
- 235000010980 cellulose Nutrition 0.000 description 12
- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 12
- 229940045145 uridine Drugs 0.000 description 12
- 241000223259 Trichoderma Species 0.000 description 11
- 241000588724 Escherichia coli Species 0.000 description 10
- HIWPGCMGAMJNRG-ACCAVRKYSA-N Sophorose Natural products O([C@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O)[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HIWPGCMGAMJNRG-ACCAVRKYSA-N 0.000 description 10
- HIWPGCMGAMJNRG-UHFFFAOYSA-N beta-sophorose Natural products OC1C(O)C(CO)OC(O)C1OC1C(O)C(O)C(O)C(CO)O1 HIWPGCMGAMJNRG-UHFFFAOYSA-N 0.000 description 10
- 229940106157 cellulase Drugs 0.000 description 10
- 239000012978 lignocellulosic material Substances 0.000 description 10
- 150000007523 nucleic acids Chemical class 0.000 description 10
- PZDOWFGHCNHPQD-VNNZMYODSA-N sophorose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](C=O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O PZDOWFGHCNHPQD-VNNZMYODSA-N 0.000 description 10
- 238000012360 testing method Methods 0.000 description 10
- 241000196324 Embryophyta Species 0.000 description 9
- 241000193385 Geobacillus stearothermophilus Species 0.000 description 9
- 238000004458 analytical method Methods 0.000 description 9
- 239000011521 glass Substances 0.000 description 9
- 108020004707 nucleic acids Proteins 0.000 description 9
- 102000039446 nucleic acids Human genes 0.000 description 9
- 239000013587 production medium Substances 0.000 description 9
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 9
- 239000006228 supernatant Substances 0.000 description 9
- 241000194108 Bacillus licheniformis Species 0.000 description 8
- 108010008885 Cellulose 1,4-beta-Cellobiosidase Proteins 0.000 description 8
- 108091071558 GH3 family Proteins 0.000 description 8
- 102000040594 GH3 family Human genes 0.000 description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 8
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 8
- 229910052799 carbon Inorganic materials 0.000 description 8
- 239000002773 nucleotide Substances 0.000 description 8
- 125000003729 nucleotide group Chemical group 0.000 description 8
- 239000007858 starting material Substances 0.000 description 8
- 229920001221 xylan Polymers 0.000 description 8
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 7
- 241000223218 Fusarium Species 0.000 description 7
- 240000008042 Zea mays Species 0.000 description 7
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 7
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 7
- 101150100570 bglA gene Proteins 0.000 description 7
- 229940041514 candida albicans extract Drugs 0.000 description 7
- 235000013339 cereals Nutrition 0.000 description 7
- 235000005822 corn Nutrition 0.000 description 7
- -1 e.g. Polymers 0.000 description 7
- 238000002203 pretreatment Methods 0.000 description 7
- 238000005215 recombination Methods 0.000 description 7
- 230000006798 recombination Effects 0.000 description 7
- 239000007787 solid Substances 0.000 description 7
- 239000012138 yeast extract Substances 0.000 description 7
- 108010011619 6-Phytase Proteins 0.000 description 6
- DLFVBJFMPXGRIB-UHFFFAOYSA-N Acetamide Chemical compound CC(N)=O DLFVBJFMPXGRIB-UHFFFAOYSA-N 0.000 description 6
- 241000228245 Aspergillus niger Species 0.000 description 6
- 241000195493 Cryptophyta Species 0.000 description 6
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 6
- 108700026244 Open Reading Frames Proteins 0.000 description 6
- 108010076504 Protein Sorting Signals Proteins 0.000 description 6
- 229920002684 Sepharose Polymers 0.000 description 6
- 108090000637 alpha-Amylases Proteins 0.000 description 6
- 238000004587 chromatography analysis Methods 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 238000002156 mixing Methods 0.000 description 6
- 230000004048 modification Effects 0.000 description 6
- 238000012986 modification Methods 0.000 description 6
- 239000013612 plasmid Substances 0.000 description 6
- 238000011218 seed culture Methods 0.000 description 6
- 239000011573 trace mineral Substances 0.000 description 6
- 235000013619 trace mineral Nutrition 0.000 description 6
- PDLPTSJWDUCMKS-UHFFFAOYSA-N 3-[4-(3-sulfopropyl)piperazin-1-yl]propane-1-sulfonic acid Chemical compound OS(=O)(=O)CCCN1CCN(CCCS(O)(=O)=O)CC1 PDLPTSJWDUCMKS-UHFFFAOYSA-N 0.000 description 5
- 241000228212 Aspergillus Species 0.000 description 5
- 235000014469 Bacillus subtilis Nutrition 0.000 description 5
- 108010084185 Cellulases Proteins 0.000 description 5
- 102000005575 Cellulases Human genes 0.000 description 5
- 102000004366 Glucosidases Human genes 0.000 description 5
- 108010056771 Glucosidases Proteins 0.000 description 5
- 239000004471 Glycine Substances 0.000 description 5
- 108091005804 Peptidases Proteins 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 5
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 5
- 238000010367 cloning Methods 0.000 description 5
- 238000010276 construction Methods 0.000 description 5
- 235000013305 food Nutrition 0.000 description 5
- 108010002430 hemicellulase Proteins 0.000 description 5
- 230000007062 hydrolysis Effects 0.000 description 5
- 238000006460 hydrolysis reaction Methods 0.000 description 5
- 239000006151 minimal media Substances 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 238000012545 processing Methods 0.000 description 5
- 230000000644 propagated effect Effects 0.000 description 5
- 150000008163 sugars Chemical class 0.000 description 5
- 230000009466 transformation Effects 0.000 description 5
- 150000004823 xylans Chemical class 0.000 description 5
- 240000006439 Aspergillus oryzae Species 0.000 description 4
- 239000002028 Biomass Substances 0.000 description 4
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 4
- 229920001503 Glucan Polymers 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 4
- 239000004365 Protease Substances 0.000 description 4
- 241000235070 Saccharomyces Species 0.000 description 4
- 241000187747 Streptomyces Species 0.000 description 4
- 241000209140 Triticum Species 0.000 description 4
- 235000021307 Triticum Nutrition 0.000 description 4
- 230000000593 degrading effect Effects 0.000 description 4
- 230000002255 enzymatic effect Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 238000010899 nucleation Methods 0.000 description 4
- 239000001965 potato dextrose agar Substances 0.000 description 4
- 239000002243 precursor Substances 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 230000014616 translation Effects 0.000 description 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 241000203069 Archaea Species 0.000 description 3
- 241000228215 Aspergillus aculeatus Species 0.000 description 3
- 241000351920 Aspergillus nidulans Species 0.000 description 3
- 241000193744 Bacillus amyloliquefaciens Species 0.000 description 3
- 102100032487 Beta-mannosidase Human genes 0.000 description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
- 101710112457 Exoglucanase Proteins 0.000 description 3
- 230000005526 G1 to G0 transition Effects 0.000 description 3
- 108010060309 Glucuronidase Proteins 0.000 description 3
- 102000053187 Glucuronidase Human genes 0.000 description 3
- 240000005979 Hordeum vulgare Species 0.000 description 3
- 235000007340 Hordeum vulgare Nutrition 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 102000004316 Oxidoreductases Human genes 0.000 description 3
- 108090000854 Oxidoreductases Proteins 0.000 description 3
- 102000035195 Peptidases Human genes 0.000 description 3
- 241000959173 Rasamsonia emersonii Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 241000223261 Trichoderma viride Species 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 239000003513 alkali Substances 0.000 description 3
- 108010084650 alpha-N-arabinofuranosidase Proteins 0.000 description 3
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 3
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 3
- 108010055059 beta-Mannosidase Proteins 0.000 description 3
- 210000004899 c-terminal region Anatomy 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 230000004186 co-expression Effects 0.000 description 3
- 239000002299 complementary DNA Substances 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 239000008121 dextrose Substances 0.000 description 3
- 239000013604 expression vector Substances 0.000 description 3
- 238000010353 genetic engineering Methods 0.000 description 3
- 238000003306 harvesting Methods 0.000 description 3
- 229940059442 hemicellulase Drugs 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 238000004949 mass spectrometry Methods 0.000 description 3
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 3
- 210000001938 protoplast Anatomy 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000010902 straw Substances 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 229920000945 Amylopectin Polymers 0.000 description 2
- 241001513093 Aspergillus awamori Species 0.000 description 2
- 241001480052 Aspergillus japonicus Species 0.000 description 2
- 101710099628 Beta-glucosidase 1 Proteins 0.000 description 2
- 206010004542 Bezoar Diseases 0.000 description 2
- 101001047514 Bos taurus Lethal(2) giant larvae protein homolog 1 Proteins 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 108091026890 Coding region Proteins 0.000 description 2
- 241000192700 Cyanobacteria Species 0.000 description 2
- 239000003155 DNA primer Substances 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 241000206602 Eukaryota Species 0.000 description 2
- 241000233732 Fusarium verticillioides Species 0.000 description 2
- 108010093031 Galactosidases Proteins 0.000 description 2
- 102000002464 Galactosidases Human genes 0.000 description 2
- 102000004157 Hydrolases Human genes 0.000 description 2
- 108090000604 Hydrolases Proteins 0.000 description 2
- 108090000769 Isomerases Proteins 0.000 description 2
- 102000004195 Isomerases Human genes 0.000 description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 2
- 240000003183 Manihot esculenta Species 0.000 description 2
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 2
- 108010054377 Mannosidases Proteins 0.000 description 2
- 102000001696 Mannosidases Human genes 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- ZRWPUFFVAOMMNM-UHFFFAOYSA-N Patulin Chemical compound OC1OCC=C2OC(=O)C=C12 ZRWPUFFVAOMMNM-UHFFFAOYSA-N 0.000 description 2
- 241000228168 Penicillium sp. Species 0.000 description 2
- KFSLWBXXFJQRDL-UHFFFAOYSA-N Peracetic acid Chemical compound CC(=O)OO KFSLWBXXFJQRDL-UHFFFAOYSA-N 0.000 description 2
- 102000003992 Peroxidases Human genes 0.000 description 2
- 241000235648 Pichia Species 0.000 description 2
- 108010059820 Polygalacturonase Proteins 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- 240000000111 Saccharum officinarum Species 0.000 description 2
- 235000007201 Saccharum officinarum Nutrition 0.000 description 2
- 108010022999 Serine Proteases Proteins 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 2
- 241000187398 Streptomyces lividans Species 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 241000223260 Trichoderma harzianum Species 0.000 description 2
- 241000378866 Trichoderma koningii Species 0.000 description 2
- 241001557886 Trichoderma sp. Species 0.000 description 2
- 241001659629 Virgibacillus Species 0.000 description 2
- MZVQCMJNVPIDEA-UHFFFAOYSA-N [CH2]CN(CC)CC Chemical group [CH2]CN(CC)CC MZVQCMJNVPIDEA-UHFFFAOYSA-N 0.000 description 2
- 238000010306 acid treatment Methods 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- 150000001413 amino acids Chemical group 0.000 description 2
- 229960000723 ampicillin Drugs 0.000 description 2
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
- 210000004102 animal cell Anatomy 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000013354 cell banking Methods 0.000 description 2
- 230000032823 cell division Effects 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000012790 confirmation Methods 0.000 description 2
- 230000001461 cytolytic effect Effects 0.000 description 2
- 239000013530 defoamer Substances 0.000 description 2
- 210000003527 eukaryotic cell Anatomy 0.000 description 2
- 108010038658 exo-1,4-beta-D-xylosidase Proteins 0.000 description 2
- 235000012041 food component Nutrition 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 230000013595 glycosylation Effects 0.000 description 2
- 238000006206 glycosylation reaction Methods 0.000 description 2
- 230000003301 hydrolyzing effect Effects 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 229920005610 lignin Polymers 0.000 description 2
- 239000002029 lignocellulosic biomass Substances 0.000 description 2
- 238000004811 liquid chromatography Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 229920001542 oligosaccharide Polymers 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 description 2
- 229940085127 phytase Drugs 0.000 description 2
- 229910000160 potassium phosphate Inorganic materials 0.000 description 2
- 235000011009 potassium phosphates Nutrition 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 235000019419 proteases Nutrition 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000004007 reversed phase HPLC Methods 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000010907 stover Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 2
- 235000020357 syrup Nutrition 0.000 description 2
- 239000006188 syrup Substances 0.000 description 2
- 239000004753 textile Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 210000005253 yeast cell Anatomy 0.000 description 2
- ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 description 1
- DBTMGCOVALSLOR-UHFFFAOYSA-N 32-alpha-galactosyl-3-alpha-galactosyl-galactose Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(OC2C(C(CO)OC(O)C2O)O)OC(CO)C1O DBTMGCOVALSLOR-UHFFFAOYSA-N 0.000 description 1
- 102100028220 ABI gene family member 3 Human genes 0.000 description 1
- 108091005508 Acid proteases Proteins 0.000 description 1
- 241001147780 Alicyclobacillus Species 0.000 description 1
- 241000609240 Ambelania acida Species 0.000 description 1
- 241001147782 Amphibacillus Species 0.000 description 1
- 229920000856 Amylose Polymers 0.000 description 1
- 241000555286 Aneurinibacillus Species 0.000 description 1
- 241001626813 Anoxybacillus Species 0.000 description 1
- 101710152845 Arabinogalactan endo-beta-1,4-galactanase Proteins 0.000 description 1
- 241001480043 Arthrodermataceae Species 0.000 description 1
- 241001494508 Arundo donax Species 0.000 description 1
- 241000956177 Aspergillus kawachii IFO 4308 Species 0.000 description 1
- 241000228257 Aspergillus sp. Species 0.000 description 1
- 241001465318 Aspergillus terreus Species 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 241000193752 Bacillus circulans Species 0.000 description 1
- 241001328122 Bacillus clausii Species 0.000 description 1
- 241000193749 Bacillus coagulans Species 0.000 description 1
- 241000006382 Bacillus halodurans Species 0.000 description 1
- 241000193422 Bacillus lentus Species 0.000 description 1
- 241000194107 Bacillus megaterium Species 0.000 description 1
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 241000193388 Bacillus thuringiensis Species 0.000 description 1
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 1
- 102100026189 Beta-galactosidase Human genes 0.000 description 1
- 241000555281 Brevibacillus Species 0.000 description 1
- 241000193764 Brevibacillus brevis Species 0.000 description 1
- 101150113278 CEL6A gene Proteins 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 101100016206 Cellvibrio japonicus (strain Ueda107) celC gene Proteins 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 241001147674 Chlorarachniophyceae Species 0.000 description 1
- 241000195628 Chlorophyta Species 0.000 description 1
- 108010023736 Chondroitinases and Chondroitin Lyases Proteins 0.000 description 1
- 241000192699 Chroococcales Species 0.000 description 1
- 241001674013 Chrysosporium lucknowense Species 0.000 description 1
- 241000123350 Chrysosporium sp. Species 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 108010062580 Concanavalin A Proteins 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 241000699802 Cricetulus griseus Species 0.000 description 1
- 240000005109 Cryptomeria japonica Species 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- RXVWSYJTUUKTEA-UHFFFAOYSA-N D-maltotriose Natural products OC1C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC(CO)C1OC1C(O)C(O)C(O)C(CO)O1 RXVWSYJTUUKTEA-UHFFFAOYSA-N 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 241000199914 Dinophyceae Species 0.000 description 1
- 241000088541 Emericella sp. Species 0.000 description 1
- 101710147028 Endo-beta-1,4-galactanase Proteins 0.000 description 1
- 101710126559 Endoglucanase EG-II Proteins 0.000 description 1
- 241000588698 Erwinia Species 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- 108090000371 Esterases Proteins 0.000 description 1
- 241000195623 Euglenida Species 0.000 description 1
- 101710098247 Exoglucanase 1 Proteins 0.000 description 1
- 101710098246 Exoglucanase 2 Proteins 0.000 description 1
- 108050000194 Expansin Proteins 0.000 description 1
- 241000220485 Fabaceae Species 0.000 description 1
- LLQPHQFNMLZJMP-UHFFFAOYSA-N Fentrazamide Chemical compound N1=NN(C=2C(=CC=CC=2)Cl)C(=O)N1C(=O)N(CC)C1CCCCC1 LLQPHQFNMLZJMP-UHFFFAOYSA-N 0.000 description 1
- 241000321606 Filobacillus Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- 241000146406 Fusarium heterosporum Species 0.000 description 1
- 241000223221 Fusarium oxysporum Species 0.000 description 1
- 241000221779 Fusarium sambucinum Species 0.000 description 1
- 241000427940 Fusarium solani Species 0.000 description 1
- 241001149959 Fusarium sp. Species 0.000 description 1
- 241000567178 Fusarium venenatum Species 0.000 description 1
- 241000896533 Gliocladium Species 0.000 description 1
- 241000768015 Gliocladium sp. Species 0.000 description 1
- 108050008938 Glucoamylases Proteins 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- 102000005744 Glycoside Hydrolases Human genes 0.000 description 1
- 108010031186 Glycoside Hydrolases Proteins 0.000 description 1
- 241001261512 Gracilibacillus Species 0.000 description 1
- HVLSXIKZNLPZJJ-TXZCQADKSA-N HA peptide Chemical compound C([C@@H](C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](C)C(O)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](N)CC=1C=CC(O)=CC=1)C1=CC=C(O)C=C1 HVLSXIKZNLPZJJ-TXZCQADKSA-N 0.000 description 1
- 241000193004 Halobacillus Species 0.000 description 1
- 101000724234 Homo sapiens ABI gene family member 3 Proteins 0.000 description 1
- 241000223198 Humicola Species 0.000 description 1
- 241000223199 Humicola grisea Species 0.000 description 1
- 241001480714 Humicola insolens Species 0.000 description 1
- 241001373560 Humicola sp. Species 0.000 description 1
- 108010003272 Hyaluronate lyase Proteins 0.000 description 1
- 102000009066 Hyaluronoglucosaminidase Human genes 0.000 description 1
- 101001035456 Hypocrea jecorina Endoglucanase-4 Proteins 0.000 description 1
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 235000014663 Kluyveromyces fragilis Nutrition 0.000 description 1
- 241000170280 Kluyveromyces sp. Species 0.000 description 1
- 241000235058 Komagataella pastoris Species 0.000 description 1
- 108010029541 Laccase Proteins 0.000 description 1
- 108010059881 Lactase Proteins 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 235000014647 Lens culinaris subsp culinaris Nutrition 0.000 description 1
- 244000043158 Lens esculenta Species 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 102000003820 Lipoxygenases Human genes 0.000 description 1
- 108090000128 Lipoxygenases Proteins 0.000 description 1
- 102000004317 Lyases Human genes 0.000 description 1
- 108090000856 Lyases Proteins 0.000 description 1
- 239000007993 MOPS buffer Substances 0.000 description 1
- 101100166758 Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) cel12A gene Proteins 0.000 description 1
- 101100166798 Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) cel3A gene Proteins 0.000 description 1
- 101710117655 Maltogenic alpha-amylase Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 108010006035 Metalloproteases Proteins 0.000 description 1
- 241000187267 Microtetraspora Species 0.000 description 1
- 240000003433 Miscanthus floridulus Species 0.000 description 1
- 241001558145 Mucor sp. Species 0.000 description 1
- 102100031688 N-acetylgalactosamine-6-sulfatase Human genes 0.000 description 1
- 230000004988 N-glycosylation Effects 0.000 description 1
- 241000221960 Neurospora Species 0.000 description 1
- 241000221961 Neurospora crassa Species 0.000 description 1
- 241000088436 Neurospora sp. Species 0.000 description 1
- 241000192522 Nostocales Species 0.000 description 1
- 102000007999 Nuclear Proteins Human genes 0.000 description 1
- 108010089610 Nuclear Proteins Proteins 0.000 description 1
- 241000320412 Ogataea angusta Species 0.000 description 1
- 241000192494 Oscillatoriales Species 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241000179039 Paenibacillus Species 0.000 description 1
- 241000194109 Paenibacillus lautus Species 0.000 description 1
- 241001520808 Panicum virgatum Species 0.000 description 1
- 241000520272 Pantoea Species 0.000 description 1
- 241000220435 Papilionoideae Species 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- 241000081271 Phaffia rhodozyma Species 0.000 description 1
- 101100172078 Phanerodontia chrysosporium Eg5A gene Proteins 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 108010064785 Phospholipases Proteins 0.000 description 1
- 102000015439 Phospholipases Human genes 0.000 description 1
- 235000014676 Phragmites communis Nutrition 0.000 description 1
- 240000000020 Picea glauca Species 0.000 description 1
- 241000235061 Pichia sp. Species 0.000 description 1
- 241000511381 Pleurocapsales Species 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- 241000168036 Populus alba Species 0.000 description 1
- 241000183024 Populus tremula Species 0.000 description 1
- 241001600128 Populus tremula x Populus alba Species 0.000 description 1
- 240000004923 Populus tremuloides Species 0.000 description 1
- 235000011263 Populus tremuloides Nutrition 0.000 description 1
- 235000015696 Portulacaria afra Nutrition 0.000 description 1
- 241000157935 Promicromonospora citrea Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000168225 Pseudomonas alcaligenes Species 0.000 description 1
- 241000589774 Pseudomonas sp. Species 0.000 description 1
- 244000046146 Pueraria lobata Species 0.000 description 1
- 235000010575 Pueraria lobata Nutrition 0.000 description 1
- 229920001218 Pullulan Polymers 0.000 description 1
- 239000004373 Pullulan Substances 0.000 description 1
- 240000009089 Quercus robur Species 0.000 description 1
- 235000011471 Quercus robur Nutrition 0.000 description 1
- 241000235403 Rhizomucor miehei Species 0.000 description 1
- 241000952054 Rhizopus sp. Species 0.000 description 1
- 241000206572 Rhodophyta Species 0.000 description 1
- 244000253911 Saccharomyces fragilis Species 0.000 description 1
- 235000018368 Saccharomyces fragilis Nutrition 0.000 description 1
- 101000906479 Saitozyma flava Endoglucanase 1 Proteins 0.000 description 1
- 101100284150 Salipaludibacillus agaradhaerens cel5A gene Proteins 0.000 description 1
- 241000235346 Schizosaccharomyces Species 0.000 description 1
- 241000235347 Schizosaccharomyces pombe Species 0.000 description 1
- 241000720795 Schizosaccharomyces sp. Species 0.000 description 1
- 244000136421 Scirpus acutus Species 0.000 description 1
- 241000209056 Secale Species 0.000 description 1
- 235000007238 Secale cereale Nutrition 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 102000012479 Serine Proteases Human genes 0.000 description 1
- 108020004459 Small interfering RNA Proteins 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 244000138286 Sorghum saccharatum Species 0.000 description 1
- 241000063122 Streptacidiphilus griseus Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 241000187759 Streptomyces albus Species 0.000 description 1
- 241000187432 Streptomyces coelicolor Species 0.000 description 1
- 241001468239 Streptomyces murinus Species 0.000 description 1
- 241000187412 Streptomyces plicatus Species 0.000 description 1
- 108090000787 Subtilisin Proteins 0.000 description 1
- 235000021536 Sugar beet Nutrition 0.000 description 1
- 241001291204 Thermobacillus Species 0.000 description 1
- 241000203640 Thermomonospora Species 0.000 description 1
- 241000204652 Thermotoga Species 0.000 description 1
- 101710097834 Thiol protease Proteins 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- 241000223262 Trichoderma longibrachiatum Species 0.000 description 1
- 241000355691 Trichoderma reesei QM9414 Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 244000177175 Typha elephantina Species 0.000 description 1
- 235000018747 Typha elephantina Nutrition 0.000 description 1
- 102000003425 Tyrosinase Human genes 0.000 description 1
- 108060008724 Tyrosinase Proteins 0.000 description 1
- 241000321595 Ureibacillus Species 0.000 description 1
- 235000006085 Vigna mungo var mungo Nutrition 0.000 description 1
- 240000005616 Vigna mungo var. mungo Species 0.000 description 1
- 108700040099 Xylose isomerases Proteins 0.000 description 1
- 108010027199 Xylosidases Proteins 0.000 description 1
- 101710185494 Zinc finger protein Proteins 0.000 description 1
- 102100023597 Zinc finger protein 816 Human genes 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 108010048241 acetamidase Proteins 0.000 description 1
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 108010028144 alpha-Glucosidases Proteins 0.000 description 1
- 102000016679 alpha-Glucosidases Human genes 0.000 description 1
- 101150069003 amdS gene Proteins 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 238000012870 ammonium sulfate precipitation Methods 0.000 description 1
- 239000001166 ammonium sulphate Substances 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000003625 amylolytic effect Effects 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000002518 antifoaming agent Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 239000010905 bagasse Substances 0.000 description 1
- 238000000498 ball milling Methods 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 108010047754 beta-Glucosidase Proteins 0.000 description 1
- 102000006995 beta-Glucosidase Human genes 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 238000010364 biochemical engineering Methods 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 239000002551 biofuel Substances 0.000 description 1
- OWMVSZAMULFTJU-UHFFFAOYSA-N bis-tris Chemical compound OCCN(CCO)C(CO)(CO)CO OWMVSZAMULFTJU-UHFFFAOYSA-N 0.000 description 1
- 235000021152 breakfast Nutrition 0.000 description 1
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 229940095731 candida albicans Drugs 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 235000020434 chocolate syrup Nutrition 0.000 description 1
- 238000011098 chromatofocusing Methods 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 108010005400 cutinase Proteins 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000037304 dermatophytes Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- YERABYSOHUZTPQ-UHFFFAOYSA-P endo-1,4-beta-Xylanase Chemical compound C=1C=CC=CC=1C[N+](CC)(CC)CCCNC(C(C=1)=O)=CC(=O)C=1NCCC[N+](CC)(CC)CC1=CC=CC=C1 YERABYSOHUZTPQ-UHFFFAOYSA-P 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 108010093305 exopolygalacturonase Proteins 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 239000013613 expression plasmid Substances 0.000 description 1
- 230000002550 fecal effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 235000021550 forms of sugar Nutrition 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 235000011868 grain product Nutrition 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000007773 growth pattern Effects 0.000 description 1
- 239000012145 high-salt buffer Substances 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 229960002773 hyaluronidase Drugs 0.000 description 1
- 238000004191 hydrophobic interaction chromatography Methods 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000003262 industrial enzyme Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 229910052816 inorganic phosphate Inorganic materials 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 108010059345 keratinase Proteins 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 229940031154 kluyveromyces marxianus Drugs 0.000 description 1
- 229940116108 lactase Drugs 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 108010062085 ligninase Proteins 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- FYGDTMLNYKFZSV-UHFFFAOYSA-N mannotriose Natural products OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(OC2C(OC(O)C(O)C2O)CO)C(O)C1O FYGDTMLNYKFZSV-UHFFFAOYSA-N 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229940127554 medical product Drugs 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 230000008986 metabolic interaction Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000003801 milling Methods 0.000 description 1
- 238000001823 molecular biology technique Methods 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 239000010813 municipal solid waste Substances 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 235000002949 phytic acid Nutrition 0.000 description 1
- 239000010908 plant waste Substances 0.000 description 1
- 238000013492 plasmid preparation Methods 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000004804 polysaccharides Chemical class 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 235000019423 pullulan Nutrition 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000008521 reorganization Effects 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 238000010563 solid-state fermentation Methods 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 108010038851 tannase Proteins 0.000 description 1
- 125000003396 thiol group Chemical class [H]S* 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 230000009105 vegetative growth Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000001238 wet grinding Methods 0.000 description 1
- 238000009279 wet oxidation reaction Methods 0.000 description 1
- 229940100445 wheat starch Drugs 0.000 description 1
- 239000010925 yard waste Substances 0.000 description 1
- FYGDTMLNYKFZSV-BYLHFPJWSA-N β-1,4-galactotrioside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@H](CO)O[C@@H](O[C@@H]2[C@@H](O[C@@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-BYLHFPJWSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161570243P | 2011-12-13 | 2011-12-13 | |
| US61/570,243 | 2011-12-13 | ||
| PCT/US2012/067717 WO2013090053A1 (en) | 2011-12-13 | 2012-12-04 | Enzyme cocktails prepared from mixed cultures |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2012352763A1 true AU2012352763A1 (en) | 2014-04-24 |
Family
ID=47352051
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2012352763A Abandoned AU2012352763A1 (en) | 2011-12-13 | 2012-12-04 | Enzyme cocktails prepared from mixed cultures |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US20140295475A1 (https=) |
| EP (1) | EP2791350B1 (https=) |
| JP (1) | JP2015500041A (https=) |
| KR (1) | KR20140122704A (https=) |
| CN (1) | CN104011222A (https=) |
| AU (1) | AU2012352763A1 (https=) |
| BR (1) | BR112014012910B1 (https=) |
| CA (1) | CA2858373A1 (https=) |
| DK (1) | DK2791350T3 (https=) |
| HK (1) | HK1203083A1 (https=) |
| MX (1) | MX367078B (https=) |
| PH (1) | PH12014500771A1 (https=) |
| RU (1) | RU2014128577A (https=) |
| WO (1) | WO2013090053A1 (https=) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX353237B (es) | 2009-09-23 | 2018-01-08 | Danisco Us Inc | Enzimas de glicosil hidrolasa novedosas y usos de las mismas. |
| RU2012131405A (ru) | 2009-12-23 | 2014-01-27 | ДАНИСКО ЮЭс ИНК. | Способы улучшения эффективности одновременно протекающих реакций осахаривания и ферментации |
| US20140134677A1 (en) | 2011-03-17 | 2014-05-15 | Danisco Us Inc. | Method for reducing viscosity in saccharification process |
| US20170183691A1 (en) * | 2014-04-21 | 2017-06-29 | Danisco Us Inc. | Simultaneous saccharification and co-fermentation of glucoamylase-expressing fungal strains with an ethanologen to produce alcohol from corn |
| CN105695439B (zh) * | 2014-11-27 | 2023-08-01 | 丰益(上海)生物技术研发中心有限公司 | 一种β-葡萄糖苷酶基因的重组表达方法 |
| EP3365458A1 (en) * | 2015-10-21 | 2018-08-29 | Novozymes A/S | Direct inoculation |
| WO2018106656A1 (en) | 2016-12-06 | 2018-06-14 | Danisco Us Inc | Truncated lpmo enzymes and use thereof |
| JP7316758B2 (ja) * | 2018-03-02 | 2023-07-28 | 合同酒精株式会社 | オフフレーバーのリスクを低減できるラクターゼ調製物 |
| CN109852557B (zh) * | 2018-11-30 | 2021-03-26 | 北京国环清华环境工程设计研究院有限公司 | 一种用于城镇存量垃圾稳定化处理的复合菌剂及其制备方法与应用 |
| MX2022002310A (es) * | 2019-08-29 | 2022-06-02 | Danisco Us Inc | Expresión de beta-glucosidasa en levadura para producción mejorada de etanol. |
| US11732278B1 (en) | 2020-03-11 | 2023-08-22 | Poet Research, Inc. | Systems and methods for co-culture of oxygen sensitive bacteria and yeast |
| US11597950B1 (en) | 2020-03-11 | 2023-03-07 | Poet Research, Inc. | Systems, compositions, and methods of fermentation with Z. mobilis |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4760025A (en) | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
| US5182204A (en) | 1984-05-29 | 1993-01-26 | Genencor International, Inc. | Non-human carbonyl hydrolase mutants, vectors encoding same and hosts transformed with said vectors |
| FR2625295B1 (fr) | 1987-12-24 | 1990-04-13 | Gaz De France | Procede et appareil destines a assurer la combustion etagee d'un melange combustible-comburant diminuant la production d'oxydes d'azote |
| US5958739A (en) | 1996-06-06 | 1999-09-28 | Genencor International Inc. | Mutant α-amylase |
| AR015977A1 (es) | 1997-10-23 | 2001-05-30 | Genencor Int | Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes |
| WO2003066826A2 (en) * | 2002-02-08 | 2003-08-14 | Genencor International, Inc. | Methods for producing ethanol from carbon substrates |
| EP1627049B1 (en) | 2003-05-29 | 2010-02-17 | Genencor International, Inc. | Novel trichoderma genes |
| CA2530641C (en) * | 2003-06-24 | 2017-02-28 | Hofer Bioreact Gmbh | Semisterile culturing of microbial mixed populations for the preparation of enzyme and metabolite mixtures |
| CN101918572A (zh) * | 2007-10-26 | 2010-12-15 | 阿伯燃料公司 | 正丁醇的生产方法 |
| US20090246844A1 (en) * | 2007-10-26 | 2009-10-01 | Arbor Fuel Inc. | Methods for the production of ethanol |
| WO2009143481A1 (en) * | 2008-05-23 | 2009-11-26 | University Of Georgia Research Foundation, Inc. | Paenibacillus ssp. and methods for fermentation of lignocellulosic materials |
| MX2010013113A (es) | 2008-06-06 | 2010-12-21 | Danisco Inc | Variantes de alfa-amilasa geobacillus stearothermophilus con propiedades mejoradas. |
| ES2621181T3 (es) * | 2008-11-21 | 2017-07-03 | Lallemand Hungary Liquidity Management Llc | Levaduras que expresan celulasas para la sacarificación y fermentación simultáneas utilizando celulosa |
| MX2011012841A (es) | 2009-06-03 | 2012-01-27 | Danisco Inc | Variantes de celulasa con expresion, actividad y/o estabilidad mejoradas y usos de las mismas. |
| EP2292731B1 (en) | 2009-08-13 | 2018-04-04 | DuPont Nutrition Biosciences ApS | Method for preparing complex cultures |
-
2012
- 2012-12-04 CA CA2858373A patent/CA2858373A1/en not_active Abandoned
- 2012-12-04 WO PCT/US2012/067717 patent/WO2013090053A1/en not_active Ceased
- 2012-12-04 RU RU2014128577A patent/RU2014128577A/ru not_active Application Discontinuation
- 2012-12-04 MX MX2014006570A patent/MX367078B/es active IP Right Grant
- 2012-12-04 BR BR112014012910-0A patent/BR112014012910B1/pt not_active IP Right Cessation
- 2012-12-04 AU AU2012352763A patent/AU2012352763A1/en not_active Abandoned
- 2012-12-04 US US14/355,835 patent/US20140295475A1/en not_active Abandoned
- 2012-12-04 CN CN201280061537.8A patent/CN104011222A/zh active Pending
- 2012-12-04 EP EP12799484.6A patent/EP2791350B1/en not_active Not-in-force
- 2012-12-04 KR KR1020147016268A patent/KR20140122704A/ko not_active Withdrawn
- 2012-12-04 PH PH1/2014/500771A patent/PH12014500771A1/en unknown
- 2012-12-04 JP JP2014547276A patent/JP2015500041A/ja not_active Withdrawn
- 2012-12-04 DK DK12799484.6T patent/DK2791350T3/da active
- 2012-12-04 HK HK15103550.9A patent/HK1203083A1/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA2858373A1 (en) | 2013-06-20 |
| EP2791350B1 (en) | 2019-01-23 |
| DK2791350T3 (da) | 2019-05-06 |
| CN104011222A (zh) | 2014-08-27 |
| JP2015500041A (ja) | 2015-01-05 |
| BR112014012910A2 (pt) | 2020-10-20 |
| WO2013090053A1 (en) | 2013-06-20 |
| KR20140122704A (ko) | 2014-10-20 |
| BR112014012910B1 (pt) | 2021-06-29 |
| MX367078B (es) | 2019-08-05 |
| RU2014128577A (ru) | 2016-02-10 |
| MX2014006570A (es) | 2014-08-21 |
| PH12014500771A1 (en) | 2021-08-09 |
| EP2791350A1 (en) | 2014-10-22 |
| US20140295475A1 (en) | 2014-10-02 |
| HK1203083A1 (en) | 2015-10-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2791350B1 (en) | Enzyme cocktails prepared from mixed cultures | |
| Manavalan et al. | Secretome analysis of Ganoderma lucidum cultivated in sugarcane bagasse | |
| Kovács et al. | Comparative enzymatic hydrolysis of pretreated spruce by supernatants, whole fermentation broths and washed mycelia of Trichoderma reesei and Trichoderma atroviride | |
| EP2598629B1 (en) | Aspergillus containing beta-glucosidase, beta-glucosidases and nucleic acids encoding the same | |
| KR20140017612A (ko) | 당화 공정에서 점도를 감소시키는 방법 | |
| KR20120106774A (ko) | 동시 당화 발효 반응의 효율을 향상시키는 방법 | |
| AU2011317171B2 (en) | Thermostable Trichoderma cellulase | |
| CN111094562A (zh) | 具有海藻糖酶活性的多肽及其在产生发酵产物的方法中的用途 | |
| CN110997701A (zh) | 具有海藻糖酶活性的多肽以及编码其的多核苷酸 | |
| Adav et al. | Proteomic analysis of temperature dependent extracellular proteins from Aspergillus fumigatus grown under solid-state culture condition | |
| EP2855673B1 (en) | Improved endoglucanases for treatment of cellulosic material | |
| Kavitha et al. | Genetic improvement of Aspergillus carbonarius for pectinase overproduction during solid state growth | |
| US20170183691A1 (en) | Simultaneous saccharification and co-fermentation of glucoamylase-expressing fungal strains with an ethanologen to produce alcohol from corn | |
| CN112771069B (zh) | 表达外源葡糖淀粉酶和木聚糖酶的酿酒酵母菌株及其在生产生物乙醇中的用途 | |
| Ilić et al. | The agricultural waste utilization for exploration of the extracellular enzyme potential of three novel white-rot Basidiomycetes: Fomes fomentarius TMF2, Schizophyllum commune TMF3 and Bjerkandera adusta TMF1 | |
| Lotfi et al. | Screening of some Zygomycetes for cellulase activity | |
| KR101650892B1 (ko) | 신규 당화효소 고활성 곰팡이 tg2 균주 및 이를 이용한 바이오에탄올 생산 방법 | |
| EP2408907B1 (en) | Use of cellulose and ligno-cellulose active proteins | |
| WO2024137704A2 (en) | Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast | |
| US9988658B2 (en) | Process for the hydrolysis of biomass | |
| KR20110070834A (ko) | 귤 표면에서 분리한 신규한 포마 종 균주 및 이로부터 생산되는 섬유소 분해 효소 및 베타글루코시다아제 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MK5 | Application lapsed section 142(2)(e) - patent request and compl. specification not accepted |