BR0214568A - Purificação cromatográfica de eritropoietina humana recombinante - Google Patents
Purificação cromatográfica de eritropoietina humana recombinanteInfo
- Publication number
- BR0214568A BR0214568A BR0214568-5A BR0214568A BR0214568A BR 0214568 A BR0214568 A BR 0214568A BR 0214568 A BR0214568 A BR 0214568A BR 0214568 A BR0214568 A BR 0214568A
- Authority
- BR
- Brazil
- Prior art keywords
- rhepo
- column
- recombinant human
- supernatant
- culture medium
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/505—Erythropoietin [EPO]
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
"PURIFICAçãO CROMATOGRáFICA DE ERITROPOIETINA HUMANA RECOMBINANTE". A invenção fornece um método para restabelecer e purificar eritropoietina humana recombinante (rhEpo) de um meio de cultura celular compreendendo células hospedeiras cujo método compreende as etapas de: (a) remover as células hospedeiras, constituintes celulares e restos do meio de cultura celular através de centrifugação usando um separador de pilha de disco seguido por uma etapa de filtração de profundidade para obter um sobrenadante do meio de cultura clarificado; (b) ajustar a condutividade do sobrenadante para 5 mS/cm ou menos, e um pH dentre cerca de 7,0 e 8,0; (c) aplicar o sobrenadante da etapa (b) a uma coluna compreendendo um meio cromatográfico de troca aniónica, lavar a coluna, eluir a rhEpo da coluna e colher a(s) fração(ões) de pico que contêm rhEpo; (d) submeter as frações de pico combinadas da etapa (c) a uma etapa de cromatografia de fase reversa usando uma resina de poliestireno que pode ser operada sob pressão média (< 10 bar) e é resistente às concentrações altas de NaOH, como Source 30RPC, a rhEpo sendo eluída usando um gradiente linear de um solvente orgânico; (e) aplicar uma ou maís frações eluídas na etapa (d) que contêm rhEpo a uma coluna compreendendo meios cromatográficos de troca aniónica Q-Seph HP, lavar a coluna e eluir a rhEpo usando um gradiente de sal linear; (f) selecionar uma ou mais frações eluídas na etapa (e) que contêm rhEpo com base no grau de sialilação da rHepo; e (g) submeter uma ou mais frações eluídas na etapa (f) que contêm rhEpo a uma ou mais etapas cromatográficas de exclusão de tamanho usando Superdex 75 "prep grade" para remover os dímeros potenciais e agregados mais altos; e colher o eluato contendo rhEpo.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US33383901P | 2001-11-28 | 2001-11-28 | |
PCT/EP2002/013299 WO2003045996A1 (en) | 2001-11-28 | 2002-11-26 | Chromatographic purification of recombinant human erythropoietin |
Publications (1)
Publication Number | Publication Date |
---|---|
BR0214568A true BR0214568A (pt) | 2004-11-03 |
Family
ID=23304479
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR0214568-5A BR0214568A (pt) | 2001-11-28 | 2002-11-26 | Purificação cromatográfica de eritropoietina humana recombinante |
Country Status (20)
Country | Link |
---|---|
US (1) | US20060099674A1 (pt) |
EP (1) | EP1453857B1 (pt) |
JP (1) | JP4293908B2 (pt) |
KR (1) | KR20040065567A (pt) |
CN (1) | CN1596268A (pt) |
AU (1) | AU2002356733A1 (pt) |
BR (1) | BR0214568A (pt) |
CA (1) | CA2466627A1 (pt) |
ES (1) | ES2525945T3 (pt) |
HR (1) | HRP20040476A2 (pt) |
HU (1) | HUP0500061A2 (pt) |
IL (1) | IL161855A0 (pt) |
MX (1) | MXPA04005189A (pt) |
NO (1) | NO20042185L (pt) |
NZ (1) | NZ533083A (pt) |
PL (1) | PL368755A1 (pt) |
RU (1) | RU2004119818A (pt) |
SI (1) | SI1453857T1 (pt) |
WO (1) | WO2003045996A1 (pt) |
ZA (1) | ZA200403461B (pt) |
Families Citing this family (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003080852A1 (en) * | 2002-03-26 | 2003-10-02 | Lek Pharmaceutical And Chemical Company D.D. | Process for the preparation of a desired erythropoietin glyco-isoform profile |
DE60228460D1 (de) * | 2002-12-13 | 2008-10-02 | Bioceuticals Arzneimittel Ag | Verfahren zur Herstellung und Reinigung von Erythropoietin |
US7157276B2 (en) * | 2003-06-20 | 2007-01-02 | Biogen Idec Inc. | Use of depth filtration in series with continuous centrifugation to clarify mammalian cell cultures |
KR100567448B1 (ko) * | 2003-11-05 | 2006-04-04 | 주식회사 인투젠 | 형질전환 동물의 유즙 전처리방법 및 이를 통한 인체단백질의 정제방법 |
DE102004027816A1 (de) | 2004-06-08 | 2006-01-05 | Bioceuticals Arzneimittel Ag | Verfahren zur Reinigung von Erythropoietin |
WO2006003926A1 (ja) * | 2004-06-30 | 2006-01-12 | Terumo Kabushiki Kaisha | ウィルス不活化ヘモグロビンおよびその製造方法 |
US7531632B2 (en) * | 2006-02-16 | 2009-05-12 | Gtc Biotherapeutics, Inc. | Clarification of transgenic milk using depth filtration |
KR100988706B1 (ko) * | 2007-03-07 | 2010-10-18 | 한미홀딩스 주식회사 | 재조합 에리트로포이에틴의 신규한 정제방법 |
KR100900033B1 (ko) * | 2007-11-21 | 2009-06-01 | 한국생명공학연구원 | 인간 에리스로포이에틴의 정제방법 |
DE102008002209A1 (de) | 2008-06-04 | 2009-12-10 | Evonik Degussa Gmbh | Verfahren zur Aufreinigung von Erythropoietin |
US8779110B2 (en) | 2008-06-24 | 2014-07-15 | Dr. Reddy's Laboratories Limited | Purification of low isoelectric point isoforms of darbepoietin |
CA2739392A1 (en) * | 2008-10-17 | 2010-04-22 | Percivia Llc | Clarification process |
DE102008054716A1 (de) * | 2008-12-16 | 2010-06-17 | Evonik Degussa Gmbh | Inprozesskontrolle in einem Verfahren zur Herstellung von EPO |
JP5728016B2 (ja) | 2009-09-23 | 2015-06-03 | ラチオファーマ ゲーエムベーハー | 組換えヒトエリスロポエチン(epo)を精製するためのプロセス、このようにして精製されたepoおよびこれを含む医薬組成物 |
WO2011063195A2 (en) * | 2009-11-20 | 2011-05-26 | Dr. Reddy's Laboratories Ltd. | Purification of modified cytokines |
CN102453087B (zh) * | 2010-10-22 | 2013-12-25 | 广东赛保尔生物医药技术有限公司 | 一种单取代peg-epo的纯化及制备方法 |
AU2011329053A1 (en) * | 2010-11-15 | 2013-06-06 | Biogen Inc. | Enrichment and concentration of select product isoforms by overloaded bind and elute chromatography |
WO2012069474A2 (en) * | 2010-11-24 | 2012-05-31 | H. Lundbeck A/S | A method for reducing potential virus burden in a sample by cyanate treatment |
KR101443257B1 (ko) | 2011-10-18 | 2014-09-19 | 주식회사 종근당 | 낮은 등전점을 갖는 에리스로포이에틴 유사체의 정제방법 |
WO2014138921A1 (en) * | 2013-03-15 | 2014-09-18 | Apotex Inc. | Enhanced liquid formulation stability of erythropoietin alpha through purification processing |
CN105358228A (zh) | 2013-07-05 | 2016-02-24 | 法国血液分割暨生化制品实验室 | 亲和层析基质 |
KR101414897B1 (ko) | 2013-11-29 | 2014-07-04 | 씨제이헬스케어 주식회사 | 다베포에틴 알파의 정제 방법 |
WO2015134640A1 (en) * | 2014-03-05 | 2015-09-11 | GlycoScientific LLC | Isotopically labeled glycans and methods for producing the same |
CA2970732C (en) | 2014-12-15 | 2023-05-16 | Merck Patent Gmbh | Target molecule capture from crude solutions |
EP3153522A1 (en) | 2015-10-09 | 2017-04-12 | Hetero Drugs Limited | Process for the purification of erythropoietin and darbepoetin alfa |
CN106770597A (zh) * | 2017-03-01 | 2017-05-31 | 上海博威生物医药有限公司 | 用于检测组合物中促红细胞生成素电荷异质体的cze检测方法 |
CN112940101A (zh) * | 2019-12-11 | 2021-06-11 | 深圳赛保尔生物药业有限公司 | 重组人促红细胞生成素的纯化方法 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4677195A (en) * | 1985-01-11 | 1987-06-30 | Genetics Institute, Inc. | Method for the purification of erythropoietin and erythropoietin compositions |
US5856298A (en) * | 1989-10-13 | 1999-01-05 | Amgen Inc. | Erythropoietin isoforms |
DE69130990T2 (de) * | 1990-02-20 | 1999-12-02 | Baxter Int | Gereinigtes, virusfreies menschliches Thrombin |
DE19504211A1 (de) * | 1995-02-09 | 1996-08-14 | Behringwerke Ag | Entfernen von Viren durch Ultrafiltration aus Proteinlösungen |
TW442492B (en) | 1995-07-26 | 2001-06-23 | Novartis Ag | Process for the enrichment of interferon |
PT889949E (pt) * | 1996-03-13 | 2003-10-31 | Delta Biotechnology Ltd | Controlo de fermentacao |
BR9917606A (pt) | 1998-11-06 | 2002-12-31 | Bio Sidus S A | Procedimento para a purificação de eritropoetina humana recombinante a partir de sobrenadantes de cultivo de células e eritropoetina humana recombinante obtida com tal procedimento |
-
2002
- 2002-11-26 MX MXPA04005189A patent/MXPA04005189A/es not_active Application Discontinuation
- 2002-11-26 HU HU0500061A patent/HUP0500061A2/hu unknown
- 2002-11-26 NZ NZ533083A patent/NZ533083A/en unknown
- 2002-11-26 IL IL16185502A patent/IL161855A0/xx unknown
- 2002-11-26 EP EP02803796.8A patent/EP1453857B1/en not_active Expired - Lifetime
- 2002-11-26 JP JP2003547445A patent/JP4293908B2/ja not_active Expired - Lifetime
- 2002-11-26 RU RU2004119818/04A patent/RU2004119818A/ru not_active Application Discontinuation
- 2002-11-26 CA CA002466627A patent/CA2466627A1/en not_active Abandoned
- 2002-11-26 SI SI200231050T patent/SI1453857T1/sl unknown
- 2002-11-26 CN CNA028236017A patent/CN1596268A/zh active Pending
- 2002-11-26 WO PCT/EP2002/013299 patent/WO2003045996A1/en active Application Filing
- 2002-11-26 PL PL02368755A patent/PL368755A1/xx unknown
- 2002-11-26 AU AU2002356733A patent/AU2002356733A1/en not_active Abandoned
- 2002-11-26 BR BR0214568-5A patent/BR0214568A/pt not_active IP Right Cessation
- 2002-11-26 US US10/494,558 patent/US20060099674A1/en not_active Abandoned
- 2002-11-26 ES ES02803796.8T patent/ES2525945T3/es not_active Expired - Lifetime
- 2002-11-26 KR KR10-2004-7008088A patent/KR20040065567A/ko not_active Application Discontinuation
-
2004
- 2004-05-06 ZA ZA200403461A patent/ZA200403461B/en unknown
- 2004-05-26 NO NO20042185A patent/NO20042185L/no not_active Application Discontinuation
- 2004-05-27 HR HR20040476A patent/HRP20040476A2/xx not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
CA2466627A1 (en) | 2003-06-05 |
NO20042185L (no) | 2004-08-23 |
SI1453857T1 (sl) | 2015-01-30 |
EP1453857B1 (en) | 2014-08-13 |
US20060099674A1 (en) | 2006-05-11 |
PL368755A1 (en) | 2005-04-04 |
IL161855A0 (en) | 2005-11-20 |
EP1453857A1 (en) | 2004-09-08 |
RU2004119818A (ru) | 2006-01-10 |
HUP0500061A2 (hu) | 2005-04-28 |
KR20040065567A (ko) | 2004-07-22 |
NZ533083A (en) | 2006-09-29 |
HRP20040476A2 (en) | 2005-10-31 |
WO2003045996A1 (en) | 2003-06-05 |
AU2002356733A1 (en) | 2003-06-10 |
MXPA04005189A (es) | 2005-02-17 |
ZA200403461B (en) | 2006-05-31 |
ES2525945T3 (es) | 2015-01-02 |
JP2005518196A (ja) | 2005-06-23 |
CN1596268A (zh) | 2005-03-16 |
JP4293908B2 (ja) | 2009-07-08 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 6A E 7A ANUIDADES. |
|
B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO PUBLICADO NA RPI 2064 DE 27/07/2010. |