BR0212649A - Sistema para alcançar elevada expressão gênica - Google Patents

Sistema para alcançar elevada expressão gênica

Info

Publication number
BR0212649A
BR0212649A BR0212649-4A BR0212649A BR0212649A BR 0212649 A BR0212649 A BR 0212649A BR 0212649 A BR0212649 A BR 0212649A BR 0212649 A BR0212649 A BR 0212649A
Authority
BR
Brazil
Prior art keywords
gene expression
achieve high
high gene
gene
recombinant vector
Prior art date
Application number
BR0212649-4A
Other languages
English (en)
Inventor
Satoshi Saitoh
Osamu Saotome
Noriko Yasutani
Yasuo Matsuo
Nobuhiro Ishida
Masana Hirai
Katsuhiko Kitamoto
Original Assignee
Toyota Motor Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP2002128323A external-priority patent/JP4109489B2/ja
Priority claimed from JP2002128286A external-priority patent/JP2003164294A/ja
Application filed by Toyota Motor Co Ltd filed Critical Toyota Motor Co Ltd
Publication of BR0212649A publication Critical patent/BR0212649A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

"SISTEMA PARA ALCANçAR ELEVADA EXPRESSãO GêNICA". A presente invenção refere-se a um método de expressar um gene, inserindo o genoma dentro de um organismo hospedeiro, utilizando-se de técnicas da engenharia genética. A presente invenção se refere, ainda, a um promotor novo, um vetor recombinante contendo o promotor e um gene alvo, um transformante contendo o vetor recombinante e um método de produção de um produto útil de gene ou substância útil que utiliza o transformante.
BR0212649-4A 2001-09-20 2002-09-13 Sistema para alcançar elevada expressão gênica BR0212649A (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2001287159 2001-09-20
JP2001286637 2001-09-20
JP2002128323A JP4109489B2 (ja) 2001-09-20 2002-04-30 遺伝子高発現系
JP2002128286A JP2003164294A (ja) 2001-09-20 2002-04-30 Ｐｄｃ１プロモーター
PCT/JP2002/009452 WO2003027280A1 (fr) 2001-09-20 2002-09-13 Systeme de surexpression genique

Publications (1)

Publication Number Publication Date
BR0212649A true BR0212649A (pt) 2004-08-24

Family

ID=27482568

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0212649-4A BR0212649A (pt) 2001-09-20 2002-09-13 Sistema para alcançar elevada expressão gênica

Country Status (7)

Country Link
US (1) US20050120394A1 (pt)
EP (1) EP1437405B1 (pt)
CN (1) CN1571838B (pt)
AU (1) AU2002330401B2 (pt)
BR (1) BR0212649A (pt)
DE (1) DE60234854D1 (pt)
WO (1) WO2003027280A1 (pt)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004104202A1 (ja) * 2003-05-22 2004-12-02 Kabushiki Kaisha Toyota Chuo Kenkyusho D-乳酸脱水素酵素活性を有するタンパク質をコードするdna及びその利用
US8071357B2 (en) * 2005-10-14 2011-12-06 Toray Industries, Inc. Yeast and method of producing L-lactic acid
JP2009034036A (ja) * 2007-08-01 2009-02-19 Toyota Motor Corp 形質転換用酵母、形質転換方法及び物質生産方法
CN104004761B (zh) * 2013-02-26 2017-11-21 中国科学院上海生命科学研究院 一种具有高效驱动活性的启动子
KR102245298B1 (ko) 2014-09-05 2021-04-27 삼성전자주식회사 증가된 edc 활성을 갖고 락테이트 생산능을 갖는 유전적으로 조작된 효모 세포, 그를 제조하는 방법, 그를 제조하는 방법, 및 그를 사용하여 락테이트를 생산하는 방법
KR102311681B1 (ko) 2015-07-28 2021-10-12 삼성전자주식회사 내산성을 갖는 효모 세포, 그를 이용하여 유기산을 생산하는 방법 및 상기 내산성 효모 세포를 생산하는 방법
CN109943492B (zh) * 2019-04-03 2022-08-16 广东省微生物研究所（广东省微生物分析检测中心） 一种重组酵母菌株及其应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1985000817A1 (en) 1983-08-10 1985-02-28 Amgen Microbial expression of interleukin ii
CA1324969C (en) * 1988-05-06 1993-12-07 Jeffrey R. Shuster High level expression of proteins in yeast
FR2693463B1 (fr) * 1992-07-08 1994-09-02 Rhone Poulenc Rorer Sa Promoteur de levure et son utilisation.
IT1294728B1 (it) * 1997-09-12 1999-04-12 Biopolo S C A R L Ceppi di lievito per la riproduzione di acido lattico
DE19919285C1 (de) * 1999-04-28 2000-10-19 Laue Hans Joachim Vorrichtung zur Fütterung und/oder zum Tränken von Nutztieren
FR2793805B1 (fr) * 1999-05-17 2003-05-02 Agronomique Inst Nat Rech PROMOTEUR DE LA THIOREDOXINE TATrxh2 DE BLE
US7141410B2 (en) * 2000-11-22 2006-11-28 Natureworks Llc Methods and materials for the production of organic products in cells of Candida species

Also Published As

Publication number Publication date
EP1437405A1 (en) 2004-07-14
DE60234854D1 (de) 2010-02-04
CN1571838A (zh) 2005-01-26
AU2002330401B2 (en) 2006-02-09
WO2003027280A1 (fr) 2003-04-03
EP1437405A4 (en) 2005-03-09
EP1437405B1 (en) 2009-12-23
CN1571838B (zh) 2010-05-26
US20050120394A1 (en) 2005-06-02

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B25A Requested transfer of rights approved

Owner name: TEIJIN LIMITED (JP)

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements