CY1107811T1 - Τροποποιημενες μονοoξυγενασες κυτοχρωματος p450 - Google Patents

Τροποποιημενες μονοoξυγενασες κυτοχρωματος p450

Info

Publication number
CY1107811T1
CY1107811T1 CY20071101499T CY071101499T CY1107811T1 CY 1107811 T1 CY1107811 T1 CY 1107811T1 CY 20071101499 T CY20071101499 T CY 20071101499T CY 071101499 T CY071101499 T CY 071101499T CY 1107811 T1 CY1107811 T1 CY 1107811T1
Authority
CY
Cyprus
Prior art keywords
modules
vectors
modified
modified watch
watch
Prior art date
Application number
CY20071101499T
Other languages
English (en)
Inventor
Bernhard Hauer
Juergen Pleiss
Ulrich Schwaneberg
Jutta Schmitt
Markus Fischer
Rolf Schmid
Qing-Shan Li
Original Assignee
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19935115A external-priority patent/DE19935115A1/de
Priority claimed from DE10011723A external-priority patent/DE10011723A1/de
Application filed by Basf Se filed Critical Basf Se
Publication of CY1107811T1 publication Critical patent/CY1107811T1/el

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/16—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing two or more hetero rings
    • C12P17/165—Heterorings having nitrogen atoms as the only ring heteroatoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/22—Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/90245—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Macromonomer-Based Addition Polymer (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Η παρούσα εφεύρεση αφορά τροποποιημένες μονοοξυγενάσες κυτοχρώματος Ρ450 με τροποποιημένο προφίλ υποστρώματος, ακολουθίες νουκλεϊνικού οξέος που κωδικεύουν κατασκευάσματα έκφρασης και φορείς, ανασυνδυασμένους μικροοργανισμούς, οι οποίοι περιέχουν τους φορείς αυτούς καθώς και μεθόδους για την μικροβιολογική παρασκευή ακροτελικά ή υπο-ακροτελικά υδροξυλιωμένων αλειφατικών καρβονικών οξέων.
CY20071101499T 1999-07-27 2007-11-22 Τροποποιημενες μονοoξυγενασες κυτοχρωματος p450 CY1107811T1 (el)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19935115A DE19935115A1 (de) 1999-07-27 1999-07-27 Elektronendonorsystem für Enzyme
DE10011723A DE10011723A1 (de) 2000-03-10 2000-03-10 Modifizierte Cytochrom P450-Monooxygenasen
EP00951461A EP1196603B1 (de) 1999-07-27 2000-07-27 Modifizierte cytochrom p450-monooxygenasen

Publications (1)

Publication Number Publication Date
CY1107811T1 true CY1107811T1 (el) 2013-06-19

Family

ID=26004785

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20071101499T CY1107811T1 (el) 1999-07-27 2007-11-22 Τροποποιημενες μονοoξυγενασες κυτοχρωματος p450

Country Status (14)

Country Link
US (2) US7531335B1 (el)
EP (2) EP1196545B1 (el)
JP (2) JP2003505066A (el)
CN (2) CN1196789C (el)
AT (2) ATE342351T1 (el)
AU (2) AU784049B2 (el)
CA (2) CA2379518A1 (el)
CY (1) CY1107811T1 (el)
DE (2) DE50013609D1 (el)
DK (1) DK1196603T3 (el)
ES (2) ES2273716T3 (el)
NO (2) NO20020350L (el)
PT (1) PT1196603E (el)
WO (2) WO2001007573A1 (el)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY126592A (en) * 1999-07-27 2006-10-31 Basf Ag Novel cytochrome p450 monooxygenases and their use for the oxidation of organic compounds
DE10051175A1 (de) 2000-10-16 2002-05-02 Basf Ag Cytochrom P450 Monoxygenasen aus thermophilen Bakterien
US7524664B2 (en) 2003-06-17 2009-04-28 California Institute Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome P450
CN100406557C (zh) * 2004-07-22 2008-07-30 浙江大学 固定化细胞色素p450 bm－3的制备方法
GB0719620D0 (en) * 2007-10-08 2007-11-14 Isis Innovation Mutant Enzymes
KR101048475B1 (ko) 2008-12-03 2011-07-11 전남대학교산학협력단 박테리아 사이토크롬 피450을 이용한 피세아타놀의 신규한 생산방법 및 이를 위한 조성물
DE102008054918A1 (de) * 2008-12-18 2010-07-01 Evonik Degussa Gmbh Verfahren zur enzymatischen Umsetzung von Alkanen
WO2011055715A1 (ja) * 2009-11-05 2011-05-12 国立大学法人神戸大学 ヒドロキシ脂肪酸類縁体およびそれらの製造方法
EP2426198A1 (en) * 2010-09-03 2012-03-07 B.R.A.I.N. Biotechnology Research And Information Network AG Cytochrome P450 monooxygenase variants
CN102399796B (zh) * 2011-11-09 2013-04-17 南京林业大学 巨大芽孢杆菌ala2细胞色素p450酶基因及其重组质粒的构建及酶的纯化方法
KR101401468B1 (ko) * 2011-11-15 2014-05-30 전남대학교산학협력단 박테리아 시토크롬 ｐ450을 이용한 아토르바스타틴으로부터 대사산물의 신규한 생산방법 및 이를 위한 조성물
US20160152957A1 (en) * 2014-11-26 2016-06-02 Invista North America S.á.r.l. Methods of Producing 6-Carbon Monomers From 8-Carbon Compounds
CN107429272A (zh) * 2014-11-26 2017-12-01 英威达纺织（英国）有限公司 用于通过c9途径产生7‑碳化合物的方法和材料
CN108004277B (zh) * 2018-01-24 2021-04-02 贵州大学 一种利用巨大芽孢杆菌制备亚油酸的方法
EP3818164B1 (en) 2018-07-03 2023-08-02 Ruhr-Universität Bochum Plasma-driven biocatalysis
US11674161B2 (en) * 2019-06-27 2023-06-13 Covestro Intellectual Property Gmbh & Co. Kg Modified monooxygenases for the manufacture of hydroxylated hydrocarbons
CN110669743A (zh) * 2019-11-08 2020-01-10 遵义医科大学 来源于抗辐射奇异球菌p450单加氧酶突变体及其应用
EP3858986A1 (en) 2020-02-03 2021-08-04 Bayer Aktiengesellschaft P450 bm3 monooxygenase variants for c19-hydroxylation of steroids
CN112359027B (zh) * 2021-01-13 2021-04-13 凯莱英生命科学技术(天津)有限公司 细胞色素p450酶突变体及其应用
GB202211941D0 (en) * 2022-08-16 2022-09-28 Univ Oxford Innovation Ltd Oxidation of steroids
CN116790529A (zh) * 2023-05-24 2023-09-22 天津大学 P450 bm3蛋白突变体及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9422205D0 (en) * 1994-11-03 1994-12-21 British Gas Plc Enzyme mutant and method
US6231746B1 (en) * 1996-05-10 2001-05-15 Bg Plc Direct electrochemistry of enzymes
WO1997049815A1 (en) * 1996-06-21 1997-12-31 Queen's University At Kingston Retinoid metabolizing protein
GB9825421D0 (en) 1998-11-19 1999-01-13 Isis Innovation Process for oxidising terpenes
MY126592A (en) * 1999-07-27 2006-10-31 Basf Ag Novel cytochrome p450 monooxygenases and their use for the oxidation of organic compounds

Also Published As

Publication number Publication date
DK1196603T3 (da) 2007-12-17
NO20020350D0 (no) 2002-01-23
AU6439000A (en) 2001-02-13
WO2001007573A1 (de) 2001-02-01
WO2001007573A8 (de) 2001-04-05
JP2003517815A (ja) 2003-06-03
AU784049B2 (en) 2006-01-19
AU6438900A (en) 2001-02-13
ES2273716T3 (es) 2007-05-16
JP2003505066A (ja) 2003-02-12
ES2291219T3 (es) 2008-03-01
CN1196789C (zh) 2005-04-13
DE50013609D1 (de) 2006-11-23
CA2379518A1 (en) 2001-02-01
EP1196603A2 (de) 2002-04-17
NO20020376L (no) 2002-03-22
DE50014590D1 (de) 2007-10-04
US7981652B2 (en) 2011-07-19
PT1196603E (pt) 2007-11-23
NO20020376D0 (no) 2002-01-24
CA2378615C (en) 2011-11-29
WO2001007574A2 (de) 2001-02-01
ATE371028T1 (de) 2007-09-15
WO2001007574A3 (de) 2001-08-09
ATE342351T1 (de) 2006-11-15
EP1196603B1 (de) 2007-08-22
CN1376194A (zh) 2002-10-23
CA2378615A1 (en) 2001-02-01
CN100482789C (zh) 2009-04-29
US20100267083A1 (en) 2010-10-21
EP1196545A1 (de) 2002-04-17
EP1196545B1 (de) 2006-10-11
CN1367835A (zh) 2002-09-04
JP4824887B2 (ja) 2011-11-30
NO20020350L (no) 2002-01-23
AU780315B2 (en) 2005-03-17
US7531335B1 (en) 2009-05-12

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