BR0012963A - Engenharia metabólica papa a produção de aminoácidos - Google Patents

Engenharia metabólica papa a produção de aminoácidos

Info

Publication number
BR0012963A
BR0012963A BR0012963-1A BR0012963A BR0012963A BR 0012963 A BR0012963 A BR 0012963A BR 0012963 A BR0012963 A BR 0012963A BR 0012963 A BR0012963 A BR 0012963A
Authority
BR
Brazil
Prior art keywords
amino acids
production
mutagenesis
microorganisms
amino acid
Prior art date
Application number
BR0012963-1A
Other languages
English (en)
Other versions
BR0012963B1 (pt
Inventor
John P Rayapati
Corey M Crafton
Original Assignee
Archer Daniels Midland Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Archer Daniels Midland Co filed Critical Archer Daniels Midland Co
Publication of BR0012963A publication Critical patent/BR0012963A/pt
Publication of BR0012963B1 publication Critical patent/BR0012963B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

"ENGENHARIA METABóLICA PARA A PRODUçãO DE AMINOáCIDOS". A presente invenção é dirigida para a produção fermentativa de aminoácidos, provendo microorganismos, métodos e processos utilizáveis. Os microorganismos da invenção são capazes de converter glucose em aminoácidos diferentes de L-isoleucina, L-leucina e L-valina com maior eficiência do que a cepa parental. A invenção provê microorganismos que são tornados auxotróficos ou braditróficos para a síntese de um ou mais aminoácidos de cadeia ramificada por mutagênese e selecionados por sua capacidade de produzir maiores rendimentos percentuais do aminoácido desejado do que a cepa parental. Os microorganismos preferidos são Corynebacterium, Brevibacterium ou Escherichia coli produzindo L-lisina. A mutagênese é realizada por técnicas clássicas ou através de metodologia de rDNA. Os métodos da invenção são projetados para aumentar a produção de um aminoácido por mutagênese de uma cepa parental, selecionando células auxotróficas ou braditróficas para a síntese de um ou mais aminoácidos de cadeia ramificada e selecionando auxotrofos ou braditrofos de aminoácido de cadeia ramificada que produzem um maior rendimento percentual a partir de dextrose do desejado aminoácido do que a cepa parental. Os processos da invenção são projetados para a produção de um aminoácido compreendendo cultivar, em um meio, um microorganismo obtido por mutagênese de uma cepa parental e selecionando variantes que são capazes de converter glucose em aminoácidos diferentes de Lisoleucina, L-leucina e L-valina com maior eficiência do que a cepa parental.
BRPI0012963-1B1A 1999-08-02 2000-08-02 engenharia metabàlica papa a produÇço de aminoÁcidos BR0012963B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14637999P 1999-08-02 1999-08-02
PCT/US2000/020979 WO2001009286A1 (en) 1999-08-02 2000-08-02 Metabolic engineering of amino acid production

Publications (2)

Publication Number Publication Date
BR0012963A true BR0012963A (pt) 2002-04-23
BR0012963B1 BR0012963B1 (pt) 2013-09-24

Family

ID=22517109

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0012963-1B1A BR0012963B1 (pt) 1999-08-02 2000-08-02 engenharia metabàlica papa a produÇço de aminoÁcidos

Country Status (12)

Country Link
US (2) US7323321B2 (pt)
EP (1) EP1200553B1 (pt)
JP (3) JP2003506030A (pt)
KR (1) KR100815041B1 (pt)
CN (1) CN1367823A (pt)
AU (1) AU779019B2 (pt)
BR (1) BR0012963B1 (pt)
CA (1) CA2380613C (pt)
DE (1) DE60042552D1 (pt)
MX (1) MXPA02001174A (pt)
NZ (1) NZ516893A (pt)
WO (1) WO2001009286A1 (pt)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10063314A1 (de) * 2000-12-20 2002-07-04 Degussa Neue für das ilvE-Gen kodierende Nukleotidsequenzen
BR0304860A (pt) * 2002-11-11 2004-08-31 Ajinomoto Kk Método para produzir uma substância alvo pela utilização de uma-bactéria pertencente ao gênero escherichia
AR058584A1 (es) 2005-12-22 2008-02-13 Takeda Pharmaceutical Derivados de metastina y uso de los mismos
PL2262894T3 (pl) 2008-03-03 2015-02-27 Global Bio Chem Tech Group Company Limited Rekombinowany mikroorganizm i sposób wytwarzania l-lizyny
DE102010003419B4 (de) 2010-03-30 2019-09-12 Evonik Degussa Gmbh Verfahren zur fermentativen Herstellung von L-Ornithin
EP3964285A1 (en) 2011-09-26 2022-03-09 Thermo Fisher Scientific Geneart GmbH High efficiency, small volume nucleic acid synthesis
IN2014CN04002A (pt) * 2011-11-02 2015-10-23 Ajinomoto Kk
CN103946371B (zh) 2011-11-02 2016-07-06 味之素株式会社 用于分泌产生蛋白质的方法
CN102604881B (zh) * 2012-03-31 2013-12-18 福建省麦丹生物集团有限公司 生产l-异亮氨酸的工程菌及其应用
US10563240B2 (en) 2013-03-14 2020-02-18 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
KR101720836B1 (ko) * 2014-08-05 2017-04-03 씨제이제일제당 (주) 피드백 저항성 아세토하이드록시산 신타아제 변이체 및 이를 이용한 l-발린의 생산방법
WO2016094512A1 (en) 2014-12-09 2016-06-16 yLIFE TECHNOLOGIES CORPORATION High efficiency, small volume nucleic acid synthesis
JP6798976B2 (ja) * 2015-02-17 2020-12-09 味の素株式会社 有機化合物又は微生物の製造システム及び製造方法
US11021697B2 (en) 2017-07-11 2021-06-01 Cj Cheiljedang Corporation Acetohydroxy acid synthase variant, microorganism comprising the same, and method of producing L-branched-chain amino acid using the same
KR101996129B1 (ko) * 2017-07-11 2019-07-04 씨제이제일제당 (주) 아세토하이드록시산 신타아제 변이체, 이를 포함하는 미생물 또는 이를 이용하는 l-분지쇄 아미노산 생산 방법
WO2019118548A1 (en) * 2017-12-15 2019-06-20 Archer Daniels Midland Company Engineered strains of corynebacteria
AU2019243241B2 (en) * 2018-03-27 2022-12-01 Cj Cheiljedang Corporation A Novel Promoter And A Method For Producing L-Amino Acid Using The Same
US20240254526A1 (en) 2018-09-07 2024-08-01 Archer Daniels Midland Company Engineered strains of corynebacteria
KR102147381B1 (ko) * 2019-11-22 2020-08-24 씨제이제일제당 주식회사 아세토하이드록시산 신타제 신규 변이체 및 이를 포함하는 미생물

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US370057A (en) * 1887-09-20 Machine for packing cube sugar in boxes
US2979439A (en) * 1958-11-04 1961-04-11 Kyowa Hakko Kogyo Kk Method of producing l-lysine by fermentation
GB1118719A (en) * 1966-03-14 1968-07-03 Kyowa Hakko Kogyo Kk Process for producing l-lysine by fermentation
IT1045451B (it) * 1966-03-23 1980-05-10 Ajinomoto Kk Metodo per recuperare lisina da brodo di fermentazione
GB1180104A (en) * 1966-06-15 1970-02-04 Ajinomoto Kk Method of Producing L-Lysine.
JPS4828078B1 (pt) * 1969-03-20 1973-08-29
JPS5544597B1 (pt) * 1969-06-09 1980-11-13
JPS5610036B1 (pt) * 1969-07-23 1981-03-05
US3825472A (en) * 1972-04-27 1974-07-23 Ajinomoto Kk Method of producing l-lysine by fermentation
JPS4981586A (pt) 1972-12-12 1974-08-06
JPS579796B2 (pt) * 1974-03-15 1982-02-23
JPS539394A (en) * 1976-07-09 1978-01-27 Kyowa Hakko Kogyo Co Ltd Preparation of l-lysine by fermentation
JPS56160997A (en) * 1980-05-16 1981-12-11 Ajinomoto Co Inc Preparation of l-lysine by fermenaition method
JPS58893A (ja) * 1981-06-25 1983-01-06 Ajinomoto Co Inc 発酵法によるｌ−イソロイシンの製造法
US5236831A (en) * 1981-12-29 1993-08-17 Kiowa Hakko Kogyo Co., Ltd. Amino acid synthesis in corynebacteria using E. coli genes
US4601983A (en) * 1983-06-15 1986-07-22 Ajinomoto Co., Inc. Coryneform bacteria carrying recombinant plasmids and their use in the fermentative production of L-threonine and L-isoleucine
DE3585052D1 (de) * 1984-06-29 1992-02-13 Ajinomoto Kk Verfahren zur herstellung von l-isoleucin durch fermentation.
EP0205849B1 (en) * 1985-05-13 1991-12-04 Toray Industries, Inc. Process for producing l-threonine by fermentation
DE3619111A1 (de) * 1986-06-06 1987-12-17 Degussa Verfahren zur fermentativen herstellung von l-isoleucin aus d,l-(alpha)-hydroxyburyrat
JPH0644872B2 (ja) * 1987-01-14 1994-06-15 三井東圧化学株式会社 発酵法によるｌ−スレオニンの製造方法
US5188948A (en) * 1987-04-16 1993-02-23 Ajinomoto Co., Inc. Process for producing L-valine by fermentation
US6261825B1 (en) * 1989-04-10 2001-07-17 Regents Of The University Of Minnesota Production of amino acids using auxotrophic mutants of methylotrophic bacillus
JP2876739B2 (ja) * 1990-08-03 1999-03-31 味の素株式会社 発酵法によるｌ―リジンの製造法
KR950005133B1 (ko) * 1990-09-18 1995-05-18 교와학꼬고오교오 가부시끼가이샤 L-발린의 제조방법
WO1995023864A1 (fr) * 1994-03-04 1995-09-08 Ajinomoto Co., Inc. Procede de production de l-lysine
JP2926991B2 (ja) * 1994-08-19 1999-07-28 味の素株式会社 発酵法によるｌ−リジン及びｌ−グルタミン酸の製造方法
JP3704737B2 (ja) * 1995-03-30 2005-10-12 味の素株式会社 Ｌ−ロイシンの製造方法
DE19547361A1 (de) * 1995-12-19 1997-06-26 Degussa Verfahren zur Herstellung von L-Aminosäuren durch Fermentation

Also Published As

Publication number Publication date
KR20020033749A (ko) 2002-05-07
AU6617400A (en) 2001-02-19
US20060154345A1 (en) 2006-07-13
US20080090272A1 (en) 2008-04-17
CA2380613C (en) 2014-03-25
CN1367823A (zh) 2002-09-04
NZ516893A (en) 2003-02-28
JP5319489B2 (ja) 2013-10-16
WO2001009286A1 (en) 2001-02-08
US7635579B2 (en) 2009-12-22
CA2380613A1 (en) 2001-02-08
DE60042552D1 (de) 2009-08-27
US7323321B2 (en) 2008-01-29
KR100815041B1 (ko) 2008-03-18
EP1200553A1 (en) 2002-05-02
JP2003506030A (ja) 2003-02-18
BR0012963B1 (pt) 2013-09-24
MXPA02001174A (es) 2002-07-30
AU779019B2 (en) 2005-01-06
JP2010022374A (ja) 2010-02-04
JP2006280383A (ja) 2006-10-19
EP1200553B1 (en) 2009-07-15

Similar Documents

Publication Publication Date Title
BR0012963A (pt) Engenharia metabólica papa a produção de aminoácidos
Ivanov et al. Biotechnology in the production of pharmaceutical industry ingredients: amino acids
Hirasawa et al. Recent advances in amino acid production by microbial cells
Sanchez et al. Our microbes not only produce antibiotics, they also overproduce amino acids
RU2140450C1 (ru) Штамм бактерий escherichia coli продуцент l-лейцина (варианты)
Lütke-Eversloh et al. Perspectives of biotechnological production of L-tyrosine and its applications
Wang et al. Metabolic engineering of l-leucine production in Escherichia coli and Corynebacterium glutamicum: a review
RU99121636A (ru) Способ получения l-аминокислот методом ферментации
JP6246894B2 (ja) Ｌ−イソロイシンを生産する微生物及びこれを用いたｌ−イソロイシン製造方法
PE20000342A1 (es) Bacteria para producir acido l-glutamico y metodo para la produccion del acido l-glutamico
Ke et al. Whole-cell conversion of l-glutamic acid into gamma-aminobutyric acid by metabolically engineered Escherichia coli
BRPI0416546A (pt) produção de ácido 3-hidroxipropiÈnico usando beta-alanina/piruvato aminotransferase
RU96110201A (ru) Способ получения веществ
RU2004106276A (ru) Способ получения целевого вещества
Wendisch Microbial production of amino acid-related compounds
CN110494553A (zh) 茶氨酸的制造方法
ES2322193T3 (es) Microorganismo del genero corynebacterium que tiene resistencia a kanamicina y productividad de l-lisina potenciada y metodo de produccion de l-lisina usando el mismo.
Enei et al. Enzymatic preparation of L-tyrosine or 3, 4-dihydroxyphenyl-L-alanine from pyruvate, ammonia and phenol or pyrocatechol
JPS62195293A (ja) 発酵法によるｌ−イソロイシンの製造法
GB2147579A (en) Production of L-amino acids from alpha-keto acids by fermentation
Ohnishi et al. Comparisons of potentials for L-lysine production among different Corynebacterium glutamicum strains
CN1053221C (zh) 用发酵法生产ｌ－苏氨酸的方法
Çalık et al. Product and by-product distributions in glutamic acid fermentation by Brevibacterium flavum: effects of the oxygen transfer
JPS63248392A (ja) 発酵法によるｌ−ロイシンの製造法
FR2601035A1 (fr) Procede de preparation de l-lysine

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06G Technical and formal requirements: other requirements [chapter 6.7 patent gazette]

Free format text: O CODIGO DE CONTROLE APRESENTADO NA PETICAO 020120033084 DE 16/04/2012 NAO CONFERE COM O GRAVADO NO CD APRESENTADO JUNTO A PETICAO.

B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 24/09/2013, OBSERVADAS AS CONDICOES LEGAIS.

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

Free format text: REFERENTE A 19A ANUIDADE.

B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2525 DE 28-05-2019 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.