BE903937A - Methode pour l'identification d'acides nucleiques. - Google Patents
Methode pour l'identification d'acides nucleiques.Info
- Publication number
- BE903937A BE903937A BE0/216059A BE216059A BE903937A BE 903937 A BE903937 A BE 903937A BE 0/216059 A BE0/216059 A BE 0/216059A BE 216059 A BE216059 A BE 216059A BE 903937 A BE903937 A BE 903937A
- Authority
- BE
- Belgium
- Prior art keywords
- identification
- nucleic acids
- affinity pair
- detection probe
- capture probe
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
Abstract
Méthode pour l'idenfication d'acides nucléiques, caractérisée en ce qu'on utilise au moins deux sondes qui sont dans la meme phase en solution dans une méthode d'hybridation, la sonde de détection étant marquée avec une substance de marquage détectable et un membre d'une paire d'affinité étant attaché à la sonde de capture, et qu'on isole l'hybride sonde de capture : acide nucléique cible : sonde de détection formé dans la réaction d'hybrication au moyen de l'autre membre de la paire d'affinité.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FI850004A FI72146C (fi) | 1985-01-02 | 1985-01-02 | Foerfarande foer identifiering av nukleinsyror. |
Publications (1)
Publication Number | Publication Date |
---|---|
BE903937A true BE903937A (fr) | 1986-04-16 |
Family
ID=8520136
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BE0/216059A BE903937A (fr) | 1985-01-02 | 1985-12-24 | Methode pour l'identification d'acides nucleiques. |
Country Status (21)
Country | Link |
---|---|
JP (1) | JPH0669400B2 (fr) |
AT (1) | AT397514B (fr) |
AU (1) | AU561382B2 (fr) |
BE (1) | BE903937A (fr) |
CA (1) | CA1271705A (fr) |
CH (1) | CH666696A5 (fr) |
DE (1) | DE3546312A1 (fr) |
DK (1) | DK164932C (fr) |
FI (1) | FI72146C (fr) |
FR (1) | FR2575493B1 (fr) |
GB (1) | GB2169403B (fr) |
HU (1) | HU196453B (fr) |
IE (1) | IE58290B1 (fr) |
IL (1) | IL77489A (fr) |
IT (1) | IT1201514B (fr) |
LU (1) | LU86238A1 (fr) |
NL (1) | NL189427C (fr) |
NO (1) | NO166743C (fr) |
RO (1) | RO94651B (fr) |
SE (1) | SE463212B (fr) |
ZA (1) | ZA859895B (fr) |
Families Citing this family (36)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5288609A (en) * | 1984-04-27 | 1994-02-22 | Enzo Diagnostics, Inc. | Capture sandwich hybridization method and composition |
US6060237A (en) * | 1985-02-26 | 2000-05-09 | Biostar, Inc. | Devices and methods for optical detection of nucleic acid hybridization |
ES8707343A1 (es) † | 1985-06-13 | 1987-07-16 | Amgen | Un metodo para aislar una secuencia de acido nucleico objetivo seleccionada |
US4868105A (en) * | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
US4910300A (en) * | 1985-12-11 | 1990-03-20 | Chiron Corporation | Method for making nucleic acid probes |
US4882269A (en) * | 1985-12-13 | 1989-11-21 | Princeton University | Amplified hybridization assay |
FI76119C (fi) * | 1986-02-27 | 1988-09-09 | Orion Yhtymae Oy | Kvantitativ bestaemning av nukleinsyramolekyler och reagensfoerpackning som anvaends vid foerfarandet. |
AU622104B2 (en) * | 1987-03-11 | 1992-04-02 | Sangtec Molecular Diagnostics Ab | Method of assaying of nucleic acids, a reagent combination and kit therefore |
WO1988010313A1 (fr) * | 1987-06-26 | 1988-12-29 | E.I. Du Pont De Nemours And Company | Extraction par affinite de sequences contaminantes a partir d'acides nucleiques clones recombinants au moyen de perles de capture |
ATE112804T1 (de) * | 1987-07-31 | 1994-10-15 | Gen Probe Inc | Polynukleotidentest unter benutzung von oligonukleotiden zur eliminierung von unerwünschten kreuzreaktionen. |
EP0305145A3 (fr) * | 1987-08-24 | 1990-05-02 | Ortho Diagnostic Systems Inc. | Méthodes et sondes pour détecter des acides nucléiques |
EP0304845A3 (fr) * | 1987-08-28 | 1991-03-06 | Profile Diagnostic Sciences Inc. | Procédé et composition pour déterminer l'expression des gènes |
CA1325761C (fr) * | 1987-12-25 | 1994-01-04 | Takanori Oka | Methode pour la detection d'un acide nucleique prevu dans un echantillon |
DE3800644A1 (de) * | 1988-01-12 | 1989-07-20 | Boehringer Mannheim Gmbh | Verfahren zum hochspezifischen nachweis von nukleinsaeuren in loesung |
US5104791A (en) * | 1988-02-09 | 1992-04-14 | E. I. Du Pont De Nemours And Company | Particle counting nucleic acid hybridization assays |
WO1989010979A1 (fr) * | 1988-05-10 | 1989-11-16 | E.I. Du Pont De Nemours And Company | Procede de detection rapide d'acide nucleique |
US5185243A (en) * | 1988-08-25 | 1993-02-09 | Syntex (U.S.A.) Inc. | Method for detection of specific nucleic acid sequences |
US5858652A (en) * | 1988-08-30 | 1999-01-12 | Abbott Laboratories | Detection and amplification of target nucleic acid sequences |
AU629845B2 (en) * | 1988-08-30 | 1992-10-15 | Abbott Laboratories | Detection and amplification of target nucleic acid sequences |
GB2225112A (en) * | 1988-11-22 | 1990-05-23 | Ici Plc | Hybridisation probes |
US5082935A (en) * | 1988-12-15 | 1992-01-21 | Amoco Corporation | Diagnostic reagents made by attaching cytidine containing nucleic acid probes to amino functionalized solid supports by bisulfite mediated transamination |
CA2025236A1 (fr) * | 1989-02-06 | 1990-08-07 | Walter King | Sondes et methodes pour la detection des listeria |
AU5269590A (en) | 1989-03-10 | 1990-10-09 | Gene-Trak Systems | Immobilized oligonucleotide probes and uses therefor |
ATE155821T1 (de) * | 1989-03-10 | 1997-08-15 | Vysis Inc | Interferenzverhinderung mit affinitätseinfangschemas |
JPH05501052A (ja) * | 1989-07-11 | 1993-03-04 | マイクロプローブ・コーポレーション | 核酸ハイブリッド形成アッセイを使用する細菌の定量 |
US5334501A (en) * | 1989-07-11 | 1994-08-02 | Microprobe Corporation | Quantification of bacteria using a nucleic acid hybridization assay |
GB8924989D0 (en) * | 1989-11-06 | 1989-12-28 | Scotgen Ltd | Method and device for the detection of antibiotic resistance |
US5580970A (en) * | 1989-12-01 | 1996-12-03 | Amoco Corporation | Detection of HPV transcripts |
AU7429591A (en) * | 1990-04-18 | 1991-10-24 | Gene-Trak Systems | Nucleic acid probes for the detection of giardia lamblia |
WO1992015708A1 (fr) * | 1991-02-27 | 1992-09-17 | Amoco Corporation | Procedes d'amelioration de la sensibilite d'essais d'hybridation |
WO1993020234A1 (fr) * | 1992-03-31 | 1993-10-14 | E.I. Du Pont De Nemours And Company | Dosage rapide, a haute capacite, fonde sur l'acide nucleique |
US7713528B1 (en) | 1993-02-18 | 2010-05-11 | Enzo Therapeutics, Inc. | Method for in vivo delivery of active compounds using reagent conjugate |
US5853993A (en) * | 1996-10-21 | 1998-12-29 | Hewlett-Packard Company | Signal enhancement method and kit |
GB0016833D0 (en) | 2000-07-07 | 2000-08-30 | Lee Helen | Improved dipstick assays (2) |
US7465540B2 (en) | 2000-09-21 | 2008-12-16 | Luminex Corporation | Multiple reporter read-out for bioassays |
CN1303221C (zh) * | 2003-01-27 | 2007-03-07 | 英科新创(厦门)科技有限公司 | 使用亲和放大的集成信号基团的靶核酸检测法 |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FI63596C (fi) * | 1981-10-16 | 1983-07-11 | Orion Yhtymae Oy | Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser |
FR2518755B1 (fr) * | 1981-12-23 | 1986-04-11 | Pasteur Institut | Sonde contenant un acide nucleique modifie et reconnaissable par des anticorps specifiques et utilisation de cette sonde pour detecter et caracteriser une sequence d'adn homologue |
GB8306426D0 (en) * | 1983-03-09 | 1983-04-13 | Malcolm A D B | Detecting polynucleotide sequence |
CA1228811A (fr) * | 1983-05-05 | 1987-11-03 | Robert G. Pergolizzi | Methode d'analyse utilisant des sequences de polynucleotides |
NO843838L (no) * | 1983-09-26 | 1985-03-27 | Ortho Diagnostic Systems Inc | Fremgangsmaate for detektering av nukleinsyre |
ZA849594B (en) * | 1983-12-12 | 1985-08-28 | Miles Lab | Nucleic acid hybridization assay employing antibodies to intercalation complexes |
FR2558172B1 (fr) * | 1984-01-16 | 1986-06-13 | Inst Nat Sante Rech Med | Sonde contenant un acide nucleique modifie et reconnaissance par des anticorps specifiques et utilisation de cette sonde et de ces anticorps specifiques pour detecter et caracteriser une sequence d'adn homologue |
CA1223222A (fr) * | 1984-02-22 | 1987-06-23 | Nanibhushan Dattagupta | Sonde moleculaire contenant un acide nucleique immobilise |
FR2560298B1 (fr) * | 1984-02-28 | 1988-07-15 | Mors | Procede et dispositif de reglage avec precision de la chute de pression d'un fluide initialement sous pression elevee, tel qu'un fluide hydraulique par exemple alimentant un groupe hydraulique, et application du procede au dispositif dans un groupe ou une centrale hydraulique, en etant integre ou non, notamment a une table de machine ou a tout dispositif de support d'outillage |
NZ211453A (en) * | 1984-03-22 | 1989-01-06 | Biotechnology Research Enterpr | Aryl azides, their preparation and use in the detection, localisation and isolation of polynucleotides |
US4766062A (en) * | 1984-05-07 | 1988-08-23 | Allied Corporation | Displacement polynucleotide assay method and polynucleotide complex reagent therefor |
-
1985
- 1985-01-02 FI FI850004A patent/FI72146C/fi not_active IP Right Cessation
- 1985-12-17 CH CH5378/85A patent/CH666696A5/de not_active IP Right Cessation
- 1985-12-20 GB GB08531414A patent/GB2169403B/en not_active Expired
- 1985-12-24 BE BE0/216059A patent/BE903937A/fr not_active IP Right Cessation
- 1985-12-27 NO NO855308A patent/NO166743C/no not_active IP Right Cessation
- 1985-12-27 JP JP60299797A patent/JPH0669400B2/ja not_active Expired - Lifetime
- 1985-12-29 HU HU855030A patent/HU196453B/hu unknown
- 1985-12-30 RO RO121637A patent/RO94651B/ro unknown
- 1985-12-30 IT IT23408/85A patent/IT1201514B/it active
- 1985-12-30 DE DE19853546312 patent/DE3546312A1/de active Granted
- 1985-12-30 FR FR858519394A patent/FR2575493B1/fr not_active Expired - Lifetime
- 1985-12-30 LU LU86238A patent/LU86238A1/fr unknown
- 1985-12-30 ZA ZA859895A patent/ZA859895B/xx unknown
- 1985-12-30 AT AT0376785A patent/AT397514B/de not_active IP Right Cessation
- 1985-12-31 CA CA000498834A patent/CA1271705A/fr not_active Expired - Lifetime
- 1985-12-31 NL NLAANVRAGE8503597,A patent/NL189427C/xx not_active IP Right Cessation
- 1985-12-31 AU AU51748/85A patent/AU561382B2/en not_active Expired
- 1985-12-31 IE IE333385A patent/IE58290B1/en not_active IP Right Cessation
- 1985-12-31 IL IL77489A patent/IL77489A/xx not_active IP Right Cessation
-
1986
- 1986-01-02 DK DK000386A patent/DK164932C/da not_active IP Right Cessation
- 1986-01-02 SE SE8600011A patent/SE463212B/sv not_active IP Right Cessation
Also Published As
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
RE20 | Patent expired |
Owner name: *SANGTEC MOLECULAR DIAGNOSTICS AB Effective date: 20051224 |