RO94651B - Metoda pentru identificarea acizilor nucleici - Google Patents

Metoda pentru identificarea acizilor nucleici

Info

Publication number
RO94651B
RO94651B RO121637A RO12163785A RO94651B RO 94651 B RO94651 B RO 94651B RO 121637 A RO121637 A RO 121637A RO 12163785 A RO12163785 A RO 12163785A RO 94651 B RO94651 B RO 94651B
Authority
RO
Romania
Prior art keywords
sample
nucleic acids
identifying
nucleic acid
support
Prior art date
Application number
RO121637A
Other languages
English (en)
Other versions
RO94651A (ro
Inventor
Erik Soderlung Hans
Original Assignee
Orion Yhtyma Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Orion Yhtyma Oy filed Critical Orion Yhtyma Oy
Publication of RO94651A publication Critical patent/RO94651A/ro
Publication of RO94651B publication Critical patent/RO94651B/ro

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)

Abstract

Inventia se refera la o metoda pentru identificarea acizilor nucleici, prin amestecarea a cel putin doua probe de acid nucleic, o proba detectoare avînd un agent de marcare si o proba de captare avînd legat de ea un membru al unei perechi de afinitate, cu o solutie care contine acidul nucleic de identificat, ajustarea volumului amestecului obtinut cu o solutie tampon si supunerea lui hibridarii, separarea hibridului format între proba detectoare, mostra si proba de captare cu ajutorul unui alt membru al perechii de afinitate fixata pe un suport si determinarea agentului de marcare a hibridului, care adera de suport.
RO121637A 1985-01-02 1985-12-30 Metoda pentru identificarea acizilor nucleici RO94651B (ro)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FI850004A FI72146C (fi) 1985-01-02 1985-01-02 Foerfarande foer identifiering av nukleinsyror.

Publications (2)

Publication Number Publication Date
RO94651A RO94651A (ro) 1988-06-30
RO94651B true RO94651B (ro) 1988-07-01

Family

ID=8520136

Family Applications (1)

Application Number Title Priority Date Filing Date
RO121637A RO94651B (ro) 1985-01-02 1985-12-30 Metoda pentru identificarea acizilor nucleici

Country Status (21)

Country Link
JP (1) JPH0669400B2 (ro)
AT (1) AT397514B (ro)
AU (1) AU561382B2 (ro)
BE (1) BE903937A (ro)
CA (1) CA1271705A (ro)
CH (1) CH666696A5 (ro)
DE (1) DE3546312A1 (ro)
DK (1) DK164932C (ro)
FI (1) FI72146C (ro)
FR (1) FR2575493B1 (ro)
GB (1) GB2169403B (ro)
HU (1) HU196453B (ro)
IE (1) IE58290B1 (ro)
IL (1) IL77489A (ro)
IT (1) IT1201514B (ro)
LU (1) LU86238A1 (ro)
NL (1) NL189427C (ro)
NO (1) NO166743C (ro)
RO (1) RO94651B (ro)
SE (1) SE463212B (ro)
ZA (1) ZA859895B (ro)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5288609A (en) * 1984-04-27 1994-02-22 Enzo Diagnostics, Inc. Capture sandwich hybridization method and composition
US6060237A (en) * 1985-02-26 2000-05-09 Biostar, Inc. Devices and methods for optical detection of nucleic acid hybridization
ES8707343A1 (es) † 1985-06-13 1987-07-16 Amgen Un metodo para aislar una secuencia de acido nucleico objetivo seleccionada
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US4910300A (en) * 1985-12-11 1990-03-20 Chiron Corporation Method for making nucleic acid probes
US4882269A (en) * 1985-12-13 1989-11-21 Princeton University Amplified hybridization assay
FI76119C (fi) * 1986-02-27 1988-09-09 Orion Yhtymae Oy Kvantitativ bestaemning av nukleinsyramolekyler och reagensfoerpackning som anvaends vid foerfarandet.
AU622104B2 (en) * 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
WO1988010313A1 (en) * 1987-06-26 1988-12-29 E.I. Du Pont De Nemours And Company Affinity removal of contaminating sequences from recombinant cloned na using capture beads
ATE112804T1 (de) * 1987-07-31 1994-10-15 Gen Probe Inc Polynukleotidentest unter benutzung von oligonukleotiden zur eliminierung von unerwünschten kreuzreaktionen.
EP0305145A3 (en) * 1987-08-24 1990-05-02 Ortho Diagnostic Systems Inc. Methods and probes for detecting nucleic acids
EP0304845A3 (en) * 1987-08-28 1991-03-06 Profile Diagnostic Sciences Inc. Method and kit for assaying gene expressions
CA1325761C (en) * 1987-12-25 1994-01-04 Takanori Oka Method of detecting an intended nucleic acid in a sample
DE3800644A1 (de) * 1988-01-12 1989-07-20 Boehringer Mannheim Gmbh Verfahren zum hochspezifischen nachweis von nukleinsaeuren in loesung
US5104791A (en) * 1988-02-09 1992-04-14 E. I. Du Pont De Nemours And Company Particle counting nucleic acid hybridization assays
WO1989010979A1 (en) * 1988-05-10 1989-11-16 E.I. Du Pont De Nemours And Company Process for rapid nucleic acid detection
US5185243A (en) * 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
US5858652A (en) * 1988-08-30 1999-01-12 Abbott Laboratories Detection and amplification of target nucleic acid sequences
AU629845B2 (en) * 1988-08-30 1992-10-15 Abbott Laboratories Detection and amplification of target nucleic acid sequences
GB2225112A (en) * 1988-11-22 1990-05-23 Ici Plc Hybridisation probes
US5082935A (en) * 1988-12-15 1992-01-21 Amoco Corporation Diagnostic reagents made by attaching cytidine containing nucleic acid probes to amino functionalized solid supports by bisulfite mediated transamination
CA2025236A1 (en) * 1989-02-06 1990-08-07 Walter King Probes and methods for the detection of listeria
AU5269590A (en) 1989-03-10 1990-10-09 Gene-Trak Systems Immobilized oligonucleotide probes and uses therefor
ATE155821T1 (de) * 1989-03-10 1997-08-15 Vysis Inc Interferenzverhinderung mit affinitätseinfangschemas
JPH05501052A (ja) * 1989-07-11 1993-03-04 マイクロプローブ・コーポレーション 核酸ハイブリッド形成アッセイを使用する細菌の定量
US5334501A (en) * 1989-07-11 1994-08-02 Microprobe Corporation Quantification of bacteria using a nucleic acid hybridization assay
GB8924989D0 (en) * 1989-11-06 1989-12-28 Scotgen Ltd Method and device for the detection of antibiotic resistance
US5580970A (en) * 1989-12-01 1996-12-03 Amoco Corporation Detection of HPV transcripts
AU7429591A (en) * 1990-04-18 1991-10-24 Gene-Trak Systems Nucleic acid probes for the detection of giardia lamblia
WO1992015708A1 (en) * 1991-02-27 1992-09-17 Amoco Corporation Methods for improving the sensitivity of hybridization assays
WO1993020234A1 (en) * 1992-03-31 1993-10-14 E.I. Du Pont De Nemours And Company A rapid, high capacity nucleic acid based assay
US7713528B1 (en) 1993-02-18 2010-05-11 Enzo Therapeutics, Inc. Method for in vivo delivery of active compounds using reagent conjugate
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
GB0016833D0 (en) 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (2)
US7465540B2 (en) 2000-09-21 2008-12-16 Luminex Corporation Multiple reporter read-out for bioassays
CN1303221C (zh) * 2003-01-27 2007-03-07 英科新创(厦门)科技有限公司 使用亲和放大的集成信号基团的靶核酸检测法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI63596C (fi) * 1981-10-16 1983-07-11 Orion Yhtymae Oy Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser
FR2518755B1 (fr) * 1981-12-23 1986-04-11 Pasteur Institut Sonde contenant un acide nucleique modifie et reconnaissable par des anticorps specifiques et utilisation de cette sonde pour detecter et caracteriser une sequence d'adn homologue
GB8306426D0 (en) * 1983-03-09 1983-04-13 Malcolm A D B Detecting polynucleotide sequence
CA1228811A (en) * 1983-05-05 1987-11-03 Robert G. Pergolizzi Assay method utilizing polynucleotide sequences
NO843838L (no) * 1983-09-26 1985-03-27 Ortho Diagnostic Systems Inc Fremgangsmaate for detektering av nukleinsyre
ZA849594B (en) * 1983-12-12 1985-08-28 Miles Lab Nucleic acid hybridization assay employing antibodies to intercalation complexes
FR2558172B1 (fr) * 1984-01-16 1986-06-13 Inst Nat Sante Rech Med Sonde contenant un acide nucleique modifie et reconnaissance par des anticorps specifiques et utilisation de cette sonde et de ces anticorps specifiques pour detecter et caracteriser une sequence d'adn homologue
CA1223222A (en) * 1984-02-22 1987-06-23 Nanibhushan Dattagupta Immobilized nucleic acid-containing probes
FR2560298B1 (fr) * 1984-02-28 1988-07-15 Mors Procede et dispositif de reglage avec precision de la chute de pression d'un fluide initialement sous pression elevee, tel qu'un fluide hydraulique par exemple alimentant un groupe hydraulique, et application du procede au dispositif dans un groupe ou une centrale hydraulique, en etant integre ou non, notamment a une table de machine ou a tout dispositif de support d'outillage
NZ211453A (en) * 1984-03-22 1989-01-06 Biotechnology Research Enterpr Aryl azides, their preparation and use in the detection, localisation and isolation of polynucleotides
US4766062A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor

Also Published As

Publication number Publication date
AU561382B2 (en) 1987-05-07
AT397514B (de) 1994-04-25
SE8600011D0 (sv) 1986-01-02
GB8531414D0 (en) 1986-02-05
NO166743B (no) 1991-05-21
FR2575493A1 (fr) 1986-07-04
GB2169403B (en) 1988-06-08
DK386A (da) 1986-07-03
FI72146C (fi) 1987-04-13
LU86238A1 (fr) 1986-04-14
DK386D0 (da) 1986-01-02
BE903937A (fr) 1986-04-16
FI850004A0 (fi) 1985-01-02
FI850004L (fi) 1986-07-03
CH666696A5 (de) 1988-08-15
IE58290B1 (en) 1993-08-25
NO855308L (no) 1986-07-03
IL77489A (en) 1991-01-31
IE853333L (en) 1986-07-02
FR2575493B1 (fr) 1990-01-26
CA1271705A (en) 1990-07-17
IT8523408A0 (it) 1985-12-30
AU5174885A (en) 1986-07-17
NL189427C (nl) 1993-04-01
SE463212B (sv) 1990-10-22
DE3546312A1 (de) 1986-07-10
HUT40166A (en) 1986-11-28
JPH0669400B2 (ja) 1994-09-07
IT1201514B (it) 1989-02-02
HU196453B (en) 1988-11-28
FI72146B (fi) 1986-12-31
JPS61185200A (ja) 1986-08-18
NL8503597A (nl) 1986-08-01
GB2169403A (en) 1986-07-09
RO94651A (ro) 1988-06-30
NO166743C (no) 1991-08-28
DE3546312C2 (ro) 1992-08-06
ATA376785A (de) 1993-09-15
SE8600011L (sv) 1986-07-03
ZA859895B (en) 1986-08-27
DK164932B (da) 1992-09-07
DK164932C (da) 1993-01-25
NL189427B (nl) 1992-11-02

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