AU726086B2 - Process for the production of a porous cross-linked polysaccharide gel and its use as a gel filtration media and in chromatography - Google Patents

Process for the production of a porous cross-linked polysaccharide gel and its use as a gel filtration media and in chromatography Download PDF

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Publication number
AU726086B2
AU726086B2 AU24178/97A AU2417897A AU726086B2 AU 726086 B2 AU726086 B2 AU 726086B2 AU 24178/97 A AU24178/97 A AU 24178/97A AU 2417897 A AU2417897 A AU 2417897A AU 726086 B2 AU726086 B2 AU 726086B2
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AU
Australia
Prior art keywords
cross
gel
polysaccharide
linked
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
AU24178/97A
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English (en)
Other versions
AU2417897A (en
Inventor
Hans Berg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Bioprocess R&D AB
Original Assignee
Amersham Pharmacia Biotech AB
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Filing date
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Application filed by Amersham Pharmacia Biotech AB filed Critical Amersham Pharmacia Biotech AB
Publication of AU2417897A publication Critical patent/AU2417897A/en
Application granted granted Critical
Publication of AU726086B2 publication Critical patent/AU726086B2/en
Assigned to GE HEALTHCARE BIO-SCIENCES AB reassignment GE HEALTHCARE BIO-SCIENCES AB Request to Amend Deed and Register Assignors: AMERSHAM PHARMACIA BIOTECH AB
Assigned to GE HEALTHCARE BIOPROCESS R&D AB reassignment GE HEALTHCARE BIOPROCESS R&D AB Alteration of Name(s) in Register under S187 Assignors: GE HEALTHCARE BIO-SCIENCES AB
Anticipated expiration legal-status Critical
Expired legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/291—Gel sorbents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26—Synthetic macromolecular compounds
    • B01J20/262—Synthetic macromolecular compounds obtained otherwise than by reactions only involving carbon to carbon unsaturated bonds, e.g. obtained by polycondensation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26—Synthetic macromolecular compounds
    • B01J20/265—Synthetic macromolecular compounds modified or post-treated polymers
    • B01J20/267—Cross-linked polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • B01J20/285—Porous sorbents based on polymers
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0036—Galactans; Derivatives thereof
    • C08B37/0039—Agar; Agarose, i.e. D-galactose, 3,6-anhydro-D-galactose, methylated, sulfated, e.g. from the red algae Gelidium and Gracilaria; Agaropectin; Derivatives thereof, e.g. Sepharose, i.e. crosslinked agarose
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3828—Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Materials Engineering (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
AU24178/97A 1996-04-11 1997-04-07 Process for the production of a porous cross-linked polysaccharide gel and its use as a gel filtration media and in chromatography Expired AU726086B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE9601368 1996-04-11
SE9601368A SE9601368D0 (sv) 1996-04-11 1996-04-11 Process for the production of a porous cross-linked polysaccharide gel
PCT/SE1997/000578 WO1997038018A1 (en) 1996-04-11 1997-04-07 Process for the production of a porous cross-linked polysaccharide gel and its use as a gel filtration media and in chromatography

Publications (2)

Publication Number Publication Date
AU2417897A AU2417897A (en) 1997-10-29
AU726086B2 true AU726086B2 (en) 2000-11-02

Family

ID=20402148

Family Applications (1)

Application Number Title Priority Date Filing Date
AU24178/97A Expired AU726086B2 (en) 1996-04-11 1997-04-07 Process for the production of a porous cross-linked polysaccharide gel and its use as a gel filtration media and in chromatography

Country Status (10)

Country Link
US (1) US6602990B1 (enExample)
EP (1) EP0892815B1 (enExample)
JP (1) JP4081143B2 (enExample)
AT (1) ATE261992T1 (enExample)
AU (1) AU726086B2 (enExample)
DE (1) DE69728141T2 (enExample)
DK (1) DK0892815T3 (enExample)
ES (1) ES2217413T3 (enExample)
SE (1) SE9601368D0 (enExample)
WO (1) WO1997038018A1 (enExample)

Families Citing this family (88)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5998606A (en) * 1997-11-10 1999-12-07 Grandics; Peter Mn(IV)-mediated crosslinking and functionalization of chromatography media
DE19803415A1 (de) * 1998-01-29 1999-08-05 Aventis Res & Tech Gmbh & Co Trennung von Stoffgemischen unter Einsatz von Polysacchariden
SE9901825D0 (sv) 1999-05-20 1999-05-20 Amersham Pharm Biotech Ab Foamed material filled with inner material
SE0004929D0 (sv) * 2000-12-29 2000-12-29 Apbiotech Ab A method for producing liquid chromatography matrices
SE0004928D0 (sv) 2000-12-29 2000-12-29 Apbiotech Ab A method for the manufacturing of porous material
CN1774292B (zh) * 2003-03-26 2012-02-22 大赛璐化学工业株式会社 色谱分离剂及其生产方法
SE0400490D0 (sv) 2004-02-26 2004-02-26 Amersham Biosciences Ab Plasmid purification
SE0402322D0 (sv) * 2004-09-22 2004-09-22 Amersham Biosciences Ab Method of preparing a chromatography matrix
JP5122958B2 (ja) * 2004-09-22 2013-01-16 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ クロマトグラフィーマトリックスの製造方法
AU2005296352B2 (en) * 2004-10-21 2011-01-06 Cytiva Bioprocess R&D Ab Chromatography ligand
AU2005317279C1 (en) * 2004-12-14 2014-07-17 Cytiva Bioprocess R&D Ab Purification of immunoglobulins
US8728828B2 (en) 2004-12-22 2014-05-20 Ge Healthcare Bio-Sciences Ab Purification of immunoglobulins
EP1848529A1 (en) * 2005-02-14 2007-10-31 GE Healthcare Bio-Sciences AB Liquid chromatography method
CA2609093C (en) * 2005-07-06 2014-12-30 Ge Healthcare Bio-Sciences Ab Method of preparing a separation matrix
SE529259C2 (sv) * 2005-08-31 2007-06-12 Ge Healthcare Bio Sciences Ab Tillverkning av kromarografimatriser, en kromarografimatris, en vätskekromatografikolonn, prcess för att isolera målföreningar och användning av en kromarografimatris för vätskekromatografi
SG131016A1 (en) * 2005-09-19 2007-04-26 Millipore Corp Asymmetric porous adsorptive bead
WO2007058599A1 (en) * 2005-11-21 2007-05-24 Ge Healthcare Bio-Sciences Ab Manufacture of a chromatography matrix
WO2007067539A2 (en) * 2005-12-05 2007-06-14 Clontech Laboratories, Inc. High density metal ion affinity compositions and methods for making and using the same
US8481298B2 (en) * 2006-09-29 2013-07-09 Ge Healthcare Bio-Sciences Ab Separation matrix for viral purification
WO2008136742A1 (en) * 2007-05-04 2008-11-13 Bio-Works Company Limited Method for the manufacture of agarose gels
WO2008146906A1 (ja) 2007-05-30 2008-12-04 Kaneka Corporation ホルミル基含有多孔質担体、それを用いた吸着体、およびそれらの製造方法
SG149759A1 (en) 2007-07-10 2009-02-27 Millipore Corp Media for affinity chromatography
AU2008279841B2 (en) * 2007-07-25 2013-05-02 Cytiva Bioprocess R&D Ab Separation matrix
US8664152B2 (en) 2007-08-31 2014-03-04 Jnc Corporation Porous cellulose gel, method for producing the same and use thereof
WO2009099375A1 (en) * 2008-02-05 2009-08-13 Ge Healthcare Bio-Sciences Ab Method for production of separation media
CN102574101A (zh) * 2009-10-12 2012-07-11 通用电气健康护理生物科学股份公司 分离基质
US9352298B2 (en) 2010-12-14 2016-05-31 Jsr Corporation Method for producing polymer particles, and polymer particles
EP2831096B1 (en) 2012-03-28 2020-04-29 GE Healthcare BioProcess R&D AB Affinity chromatography matrix
JP6397422B2 (ja) 2012-11-13 2018-09-26 ジーイー・ヘルスケア・バイオプロセス・アールアンドディ・アクチボラグ マルチモーダルアニオン交換マトリックス
JP6348507B2 (ja) 2012-12-14 2018-06-27 ジーイー・ヘルスケア・バイオプロセス・アールアンドディ・アクチボラグ 充填ベッドクロマトグラフィーカラムの洗浄方法
AU2013368247B2 (en) * 2012-12-28 2017-03-09 Nalco Company Chemical treatment to improve red mud separation and washing in the Bayer Process
JPWO2014157606A1 (ja) * 2013-03-29 2017-02-16 国立大学法人京都大学 架橋された疎水化多糖ナノゲル粒子とその製造方法
EP3459631A1 (en) 2013-09-27 2019-03-27 Kaneka Corporation Process for producing porous cellulose beads using alkali aqueous solution and porous cellulose beads produced by this process
EP3055060A4 (en) * 2013-10-10 2017-05-24 GE Healthcare BioProcess R&D AB Method for production of a chromatography material
JP5883068B2 (ja) * 2014-05-19 2016-03-09 株式会社カネカ 多孔質担体、およびそれを用いた精製用吸着体、およびそれらの製造方法、およびそれらを用いた精製方法
EP3160982B1 (en) 2014-06-24 2020-10-28 Cytiva BioProcess R&D AB Chromatography method
US11566082B2 (en) 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
CN106140038B (zh) * 2015-04-08 2019-07-12 通用电气公司 制备聚合物微粒的方法和装置
GB201509677D0 (en) * 2015-06-04 2015-07-22 Ge Healthcare Bio Sciences Ab Manufacturing process for polysaccharide beads
GB201515339D0 (en) 2015-08-28 2015-10-14 Ge Healthcare Bio Sciences Ab A seperation matrix and a method of seperating antibodies
US10427950B2 (en) 2015-12-04 2019-10-01 Ecolab Usa Inc. Recovery of mining processing product using boronic acid-containing polymers
WO2017174422A1 (en) 2016-04-06 2017-10-12 Ge Healthcare Bioprocess R&D Ab Chromatography matrix
JP7031934B2 (ja) 2016-05-11 2022-03-08 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックス
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
US10654887B2 (en) * 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
CN109311949B (zh) 2016-05-11 2022-09-16 思拓凡生物工艺研发有限公司 储存分离基质的方法
JP6987424B2 (ja) 2016-05-11 2022-01-05 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックスを洗浄および／または消毒する方法
US10703774B2 (en) * 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
WO2017194597A1 (en) 2016-05-11 2017-11-16 Ge Healthcare Bioprocess R&D Ab Separation matrix
CN107537412B (zh) 2016-06-27 2021-06-01 思拓凡生物工艺研发有限公司 制备聚合物微球的方法和系统
US12448411B2 (en) 2016-09-30 2025-10-21 Cytiva Bioprocess R&D Ab Separation method
GB201703116D0 (en) 2017-02-27 2017-04-12 Ge Healthcare Bioprocess R&D Ab A seperation matrix and a method of seperating antibodies
GB201708277D0 (en) 2017-05-24 2017-07-05 Ge Healthcare A Recombinant protein
GB201710130D0 (en) 2017-06-26 2017-08-09 Ge Healthcare Bioprocess R & D Ab A Method of seperation
CN111148753B (zh) 2017-09-25 2024-02-23 Jsr株式会社 免疫球蛋白结合蛋白质和使用其的亲和载体
TW201928059A (zh) 2017-09-25 2019-07-16 日商Ｊｓｒ股份有限公司 免疫球蛋白結合蛋白質，及使用其之親和性載體
GB2573133A (en) * 2018-04-25 2019-10-30 Ge Healthcare Bioprocess R&D Ab Separation matrix and method of separation
GB201810690D0 (en) 2018-06-29 2018-08-15 Ge Healthcare Bioprocess R&D Ab Chromatography beads, production and use thereof
WO2020040307A1 (ja) 2018-08-24 2020-02-27 Jsr株式会社 イムノグロブリン結合タンパク質、及びそれを用いたアフィニティー担体
GB201820806D0 (en) 2018-12-20 2019-02-06 Ge Healthcare Bioprocess R&D Ab Large pore agarose
JP7715446B2 (ja) 2019-04-29 2025-07-30 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ プロテインＡと結合する能力があるＦｃ領域を欠いている抗体又は抗体断片の分離のための方法
GB201905919D0 (en) 2019-04-29 2019-06-12 Ge Healthcare Bioprocess R & D Ab Method of manufacturing agar or agarose beads
GB202018588D0 (en) 2020-11-26 2021-01-13 Cytiva Bioprocess R & D Ab Separation matrix
WO2022174005A1 (en) 2021-02-12 2022-08-18 Ecolab Usa Inc. Purification of bauxite ores using boronic acid-functional compounds
GB202109246D0 (en) 2021-06-28 2021-08-11 Cytiva Bioprocess R & D Ab A method of separating bispecific antibodies
GB202110014D0 (en) 2021-07-12 2021-08-25 Cytiva Bioprocess R & D Ab A method for separating adeno-associated virus capsids, compositions obtained by said method and uses thereof
SE545486C2 (en) 2021-07-15 2023-09-26 Bio Works Tech Ab Method for the manufacture of agar or agarose beads using vegetable oil
GB202113626D0 (en) 2021-09-24 2021-11-10 Cytiva Bioprocess R & D Ab FC binding polypeptides
EP4444462A1 (en) 2021-12-07 2024-10-16 Cytiva BioProcess R&D AB Separation matrix and methods for separating target molecules
GB202202777D0 (en) 2022-03-01 2022-04-13 Cytiva Bioprocess R & D Ab A method for separating supercoiled plasmid DNA
GB202203478D0 (en) 2022-03-14 2022-04-27 Cytiva Bioprocess R & D Ab Vh3 binding polypeptides
GB202203640D0 (en) 2022-03-16 2022-04-27 Cytiva Bioprocess R & D Ab Alkali-stabilized kappa light chain-binding separation matrix
GB202206123D0 (en) 2022-04-27 2022-06-08 Cytiva Bioprocess R & D Ab A pre-screening method and a method for separating adeno-associated virus capsids
GB202214280D0 (en) 2022-09-29 2022-11-16 Cytiva Vioprocess R&D Ab Chromatography ligand and chromatography material, and uses thereof
GB202215814D0 (en) 2022-10-26 2022-12-07 Cytiva Bioprocess R & D Ab A chromatography device, system, and use thereof for analytic separation
WO2024217979A1 (en) 2023-04-19 2024-10-24 Cytiva Bioprocess R&D Ab A method for purification of extracellular vesicles or enveloped viruses by means of size exclusion chromatography
WO2024256192A1 (en) 2023-06-13 2024-12-19 Cytiva Bioprocess R&D Ab Purification of enveloped or membranous biological particles
US12312447B2 (en) 2023-06-19 2025-05-27 Zhejiang Triple Win New Material Co., Ltd. Preparation method for polysaccharide spherical gel resin
WO2025036605A1 (en) 2023-08-14 2025-02-20 Cytiva Bioprocess R&D Ab Vh3 binding separation matrix and methods for using the same
WO2025049869A1 (en) 2023-09-01 2025-03-06 Purolite Llc Separation matrices and methods for liquid chromatography with improved linear flow velocity
WO2025056387A1 (en) 2023-09-12 2025-03-20 Cytiva Bioprocess R&D Ab A support material for solid-phase synthesis of oligonucleotides and peptides
KR102741170B1 (ko) * 2023-10-13 2024-12-10 퓨리오젠 주식회사 다공성 아가 또는 아가로스 비드 및 이의 제조방법
EP4541444A1 (en) 2023-10-20 2025-04-23 Cytiva BioProcess R&D AB Fusion protein for affinity capture
WO2025083267A1 (en) 2023-10-20 2025-04-24 Cytiva Bioprocess R&D Ab Aav9 binding polypeptides
EP4541814A1 (en) 2023-10-20 2025-04-23 Cytiva BioProcess R&D AB Stabilized polypeptides
WO2025186218A1 (en) 2024-03-04 2025-09-12 Cytiva Bioprocess R&D Ab Purification and analysis of rna
WO2025202371A1 (en) 2024-03-27 2025-10-02 Cytiva Bioprocess R&D Ab Preparation of circular rna

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2247943A1 (de) * 1971-10-01 1973-04-26 Orion Yhtymae Oy Verfahren zum vernetzen von agarose oder agar
US3873514A (en) 1974-05-17 1975-03-25 Bio Rad Laboratories Preparation of gel for affinity chromatography
SE449225B (sv) * 1980-12-18 1987-04-13 Gelinnovation Hb Gelprodukt baserad pa partiklar innehallande galaktan for separationsendamal, molekyl- och partikelimmobilisering
SE8205908D0 (sv) * 1982-10-18 1982-10-18 Pharmacia Diagnostics Ab Sett att bilda polymerskikt
SE437270B (sv) * 1983-07-19 1985-02-18 Pharmacia Ab Separationsmaterial av tverbunden agaros och dess framstellning separationsmaterial av tverbunden agaros och dess framstellning
US4582865A (en) * 1984-12-06 1986-04-15 Biomatrix, Inc. Cross-linked gels of hyaluronic acid and products containing such gels
SE458525B (sv) * 1985-05-23 1989-04-10 Pharmacia Ab Foerfarande foer tvaerbindning av en poroes agar- eller agarosgel
CA1329800C (en) * 1987-12-29 1994-05-24 Hiroaki Takayanagi Composite separating agent
AU3124793A (en) * 1991-10-29 1993-06-07 Clover Consolidated, Limited Crosslinkable polysaccharides, polycations and lipids useful for encapsulation and drug release
SE9200827D0 (sv) * 1992-03-18 1992-03-18 Pharmacia Lkb Biotech Supet porous polysaccharide gels
US5371208A (en) * 1992-12-30 1994-12-06 Guest Elchrom Scientific Ltd. Preparation of cross-linked linear polysaccharide polymers as gels for electrophoresis
US5785832A (en) * 1995-07-26 1998-07-28 Chiari; Marcella Covalently cross-linked, mixed-bed agarose-polyacrylamide matrices for electrophoresis and chromatography
US5840877A (en) * 1996-09-05 1998-11-24 Guest Elchrom Scientific Electrophoresis gels of enhanced selectivity
SE9700769D0 (sv) * 1997-03-04 1997-03-04 Pharmacia Biotech Ab Matriser för separation och separation som utnyttjar matriserna

Also Published As

Publication number Publication date
JP4081143B2 (ja) 2008-04-23
WO1997038018A1 (en) 1997-10-16
ES2217413T3 (es) 2004-11-01
US6602990B1 (en) 2003-08-05
ATE261992T1 (de) 2004-04-15
AU2417897A (en) 1997-10-29
DE69728141D1 (de) 2004-04-22
SE9601368D0 (sv) 1996-04-11
JP2000508361A (ja) 2000-07-04
EP0892815B1 (en) 2004-03-17
DE69728141T2 (de) 2005-02-17
EP0892815A1 (en) 1999-01-27
DK0892815T3 (da) 2004-06-21

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