AU2020102547A4

AU2020102547A4 - A Natural Fodder Additive with Antidiarrheal, Antibacterial and Anti-inflammatory Effects and Its Preparation Method

Info

Publication number: AU2020102547A4
Application number: AU2020102547A
Authority: AU
Inventors: Xiangqian Liu
Original assignee: Changsha Broad Ocean Bio Science And Technique Co Ltd
Current assignee: Changsha Broad-Ocean Bio-Science And Technique Co Ltd
Priority date: 2020-10-01
Filing date: 2020-10-01
Publication date: 2020-11-19
Anticipated expiration: 2028-10-01

Abstract

The invention provides a natural fodder additive with antidiarrheal, antibacterial and anti inflammatory effects, which is composed of main drug and optional existing adjuvant composition. The composition of the main drug, which is divided by weight, includes 5~40 parts of Herba Erodii seu Geranii, 5~40 parts of Herba Houttuyniae, 2-15 parts of Radix Sanguisorbae, 2~35 parts of Galla Chinensis, 2~20 parts of Radix Berberidis or Cortex Phellodendri, and 1-20 parts of Radix Glycyrrhizae, and might also includes 0.5~10 parts of Radix Scutellariae, 1-8 parts of Herba Polygoni Perfoliati, 5~10 parts of Pileostegia viburnoides Hook. f. et Thoms., 2~30 parts of Herba Portulacae, and 2~18 parts of Cortex Acanthopanax Radicis (root, stem and/or leaf). The fodder additive can be used to prevent or treat respiratory or intestinal diseases of domestic fowl and livestock, especially pneumonia or dysentery.

Description

A Natural Fodder Additive with Antidiarrheal, Antibacterial and Anti-inflammatory Effects and Its Preparation Method

TECHNICAL FIELD

[0001] The patent for invention relates to the field of fodder additive. In particular, it provides a natural fodder additive with antidiarrheal, antibacterial and anti inflammatory effects. The patent for invention also relates to the preparation method of this natural fodder additive.

BACKGROUND

[0002] If domestic fowl and livestock are infected with respiratory diseases, pneumonia, enteritis, dysentery and other diseases, it will cause great economic losses to farmers. The traditional fodder additive is usually added the enrofloxacin, ciprofloxacin or ofloxacin and other antibiotics to prevent and control fowl and livestock diseases, which is easy to remain in meat, therefore having a harmful influence on the human body. With the improvement of people's living standards, pork, chicken, beef with antibiotic residues are increasingly rejected by the world. Many countries in the world have proposed provisions to gradually eliminate or prohibit the application of antibiotics and chemical additives. What the public need is high quality meat product which is safe, health and has no antibiotics. Therefore, it is urgent to figure out the new fodder additive that is green and healthy without antibiotics or residues, and has antidiarrheal, antibacterial and anti-inflammatory effects.

[0003] The immunologic active materials of Chinese herbs and natural plants can enhance the body immune function of human body, and have thousands of years of clinical application basis. With the further in-depth work of modem scientific research, it is found that Chinese herbs and some natural products have the ability to improve anti-stress and anti-virus functions of animals, improve animal meat quality and production performance and is non-toxic and non-residue. The use of Chinese herbs and natural products has a good theoretical basis and great application prospects for the development of new fodder additives without antibiotics. At present, however, the development of Chinese herbal fodder additives are mostly based on ancient Chinese medicine, which is directly crushed and simply processed before application, having the disadvantages of large dosage, and lack of theoretical basis of farm feeding.

[0004] Although the development of pure Chinese herbal fodder additives has made a definite progress at present, each product has both the advantages and disadvantages, and in comparison with antibiotics, pure Chinese herbal fodder additives generally appear to take effect slowly, the disease-resistant effect is not idea, and the potency ratio has no advantages. In the current field of fodder additives, is has not established an irreplaceable position.

SUMMARY

[0005] The purpose of the invention is to provide a natural fodder additive with advantages of high bioavailability, low dosage, quick effect, high stability, clear mode of effect, and antidiarrheal, antibacterial and anti-inflammatory effects for the prevention and treatment of respiratory diseases, pneumonia, diarrhea and dysentery in domestic fowl and livestock, which contains no antibiotics, hormones or chemicals, so as to solve the disadvantages of the above background technology.

[0006] Another purpose of the invention is to provide the preparation method and use of the fodder additive.

[0007] The technical scheme of the invention is as follows:

[0008] The invention provides a natural fodder additive with antidiarrheal, antibacterial and anti-inflammatory effects, which is composed of main drug and optional existing adjuvant composition, and is characterized in that the main drug is composed of Herba Erodii seu Geranii, Herba Houttuyniae, Radix Sanguisorbae, Galla Chinensis, Radix Berberidis or Cortex Phellodendri, and Radix Glycyrrhizae. Divided by weight, it contains 5~40 parts of Herba Erodii seu Geranii, 5~40 parts of Herba Houttuyniae, 2-15 parts of Radix Sanguisorbae, 2-35 parts of Galla Chinensis, 2-20 parts of Radix Berberidis or Cortex Phellodendri, and 1-20 parts of Radix Glycyrrhizae.

[0009] The main drug of the fodder additive mentioned above can also be added 0.5-10 parts of Radix Scutellariae, 1-8 parts of Herba Polygoni Perfoliati, 5-10 parts of Pileostegia viburnoides Hook. f. et Thoms., 2-30 parts of Herba Portulacae and 2-18 parts of Cortex Acanthopanax Radicis (root, stem and/or leaf), which is divided by weight.

[0010] Preferably, the invention provides a fodder additive mentioned above, of which the main drugs include: 14 parts of Herba Erodii seu Geranii, 23 parts of Herba Houttuyniae, 20 parts of Radix Sanguisorbae, 17 parts of Galla Chinensis, 20 parts of Radix Berberidis or Cortex Phellodendri, and 6 parts of Radix Glycyrrhizae.

[0011] Preferably, the invention provides a fodder additive mentioned above, of which the main drugs include: 10 parts of Herba Erodii seu Geranii, 12 parts of Herba Houttuyniae , 14 parts of Radix Sanguisorbae, 12 parts of Galla Chinensis, 12 parts of Cortex Phellodendri, 4 parts of Radix Glycyrrhizae, 4 parts of Radix Scutellariae, 6 parts of Herba Polygoni Perfoliati, 6 parts of Pileostegia viburnoides Hook. f. et Thoms., 12 parts of Herba Portulacae and 8 parts of Cortex Acanthopanax Radicis (root, stem and/or leaf).

[0012] The fodder additive described above optionally comprises adjuvant composition, namely 0-52 parts of adjuvant composition, which is one or more of wheat bran, rice bran, dextrin, starch and wheat middlings.

[0013] The fodder additive mentioned in the invention has definite antidiarrheal, antibacterial or anti-inflammatory effects and can be used to prevent or treat respiratory or intestinal diseases of domestic fowl and livestock, especially pneumonia or dysentery. The fowl might be laying hen, dorking, duck, goose, or pigeon; The livestock might be pig, cattle, or sheep.

[0014] The invention also provides the preparation method of the natural fodder additive mentioned above, which is characterized in that:

[0015] a. Herba Erodii seu Geranii, Radix Sanguisorbae, Galla Chinensis, Radix Berberidis, Radix Glycyrrhizae, Radix Scutellariae, Herba Polygoni Perfoliati, Pileostegia viburnoides Hook. f. et Thoms., Herba Portulacae, Cortex Acanthopanax Radicis (root, stem and/or leaf) and Cortex Phellodendri are extracted with solvent respectively or in mixture. The extracting solution is collected, and the extractive is obtained by removing the solvent;

[0016] b. Herba Houttuyniae is extracted with water flash method. The extracting solution is collected, and the extractive is obtained by removing the solvent;

[0017] c. The extractive obtained from step a and b is dried into the dry paste, and then crushed and optionally added the adjuvant composition followed by uniform mixing.

[0018] Or the natural fodder additive mentioned in the invention is prepared in the method of:

[0019] a. Extracting the volatile oil from Herba Houttuyniae, making the volatile oil into cyclodextrin inclusion complex, keeping the dregs of decoction in reserve;

[0020] b. Herba Erodii seu Geranii, Radix Sanguisorbae, Galla Chinensis, Radix Berberidis, Radix Glycyrrhizae, Radix Scutellariae, Herba Polygoni Perfoliati, Pileostegia viburnoides Hook. f. et Thoms., Herba Portulacae, Cortex Acanthopanax Radicis (root, stem and/or leaf) and Cortex Phellodendri, together with the dregs of decoction obtained from step a, are extracted with solvent respectively or in mixture. The extracting solution is collected, and the extractive is obtained by removing the solvent;

[0021] c. The extractive obtained from step b is dried into the dry paste, and then crushed and optionally added the adjuvant composition and cyclodextrin inclusion complex obtained in step a followed by uniform mixing.

[0022] The natural fodder additive mentioned in the invention can also be used directly after the raw material of the medicine material is crushed, namely the main drug materials are mixed separately or in mixture, and then crushed and optionally added the adjuvant composition followed by uniform mixing.

[0023] The Pileostegia viburnoides used in the fodder additive of the invention is Pileostegia vibumoides Hook. f. et Thoms., a plant of the genus Polygonaceae, alias green cotton rattan, bamboo hemp, monkey head rattan, paper kato, monkey big rope. The distribution in China is mainly in Anhui, Zhejiang, Jiangxi, Hunan, Hubei, Sichuan, Guizhou, Fujian, Taiwan, Guangdong, Guangxi, Yunnan, and Hainan areas. The function of the Pileostegia viburnoides Hook. f. et Thoms. is mainly for getting rid of rheumatism, promoting blood circulation and dispersing blood stasis, eliminating swelling and relieving pain and bone grafting; The root and stem are used for waist and leg pain, rheumatism numbness. External use to treat injury, fracture, traumatic bleeding, and sore poison. The applied part of Pileostegia viburnoides Hook. f. et Thoms. of the invention might be part of or the whole of root, stem and leaf.

[0024] In the preparation method of the fodder additive mentioned above, the solvent used for solvent extraction may be water or ethanol of different concentrations, for example, the ethanol solution with a concentration of 50%.

[0025] In the preparation method of the fodder additive mentioned above, the extraction may be one or more of the methods of heating reflux extraction, ultrasonic extraction, microwave extraction and flash extraction.

[0026] Flash extraction is a well-known extraction technology in the field of extraction of traditional Chinese medicine. It relies on high-speed mechanical shear force and ultra-active molecular filtration technology to crush the plant material into fine particles at room temperature and in the presence of solvent within a few seconds, and the effective components can quickly achieve the goal of preventing internal and external balance, and the extraction can be achieved through filtration. The extraction method can keep the effective components of the plant to the maximum extent, and will not be destroyed by heat. It has the advantages of normal temperature extraction, less solvent dosage, short extraction time, high efficiency and simple operation steps.

[0027] In the preparation method of the fodder additive of the invention, the extractive is dried into the dry paste, wherein drying can be one or more of baking, spray drying and microwave vacuum drying.

[0028] Microwave vacuum drying is a drying method in which the material is placed under negative pressure and the boiling point under negative pressure is reached by microwave heating, or through melting point after solidification by cooling so as to dry materials. Because the microwave heating used in this technology is a kind of radiation heating, it is the direct action of microwave on the object, the material is heated inside and outside at the same time, and the heat is not transmitted by convection or conduction. So the heating speed is fast, the heat efficiency is high, the treatment time is short, the temperature inside and outside of the material is uniform, and it has the advantages of saving energy, high drying efficiency, high drying quality and does not break the effective constituent.

[0029] The recommended dose of the fodder additive used in domestic fowl and livestock is 0.1-0.3% of the proportion of the daily ration.

[0030] The advantages of this patent for invention: The raw materials of this patent for invention for natural fodder additive are all from natural Chinese herbs, which maintain the natural state and biological activity of various composition structures, are harmless to people and animals, and are scientifically processed before application to remove harmful parts, maintain pure nature, and have unique advantages in the prevention and treatment of fowl and livestock diseases. The toxic side effects and drug resistance of traditional chemicals, antibiotics and hormones are easy to cause drug residues in animal products, which has become a concern of the whole society. The toxic side effects of this natural fodder additive are small, which can avoid "3 causes" (mutation, teratogenicity carcinoma), residual and drug resistance problems, which can specifically or non-specifically enhance the immunity of animal body. It is the unique advantage of this additive, which existing products can not be matched with. The invention can not only prevent diarrheal and dysentery diseases, but also make all-round coordination of Chinese herbal effects and the whole mobilization of the beneficial factors to the body, and also can improve the lean meat rate of the pig, reduce the water loss rate and increase the tenderness of the meat. The preparation process of the invention is relatively simple, the production does not pollute the environment, the various chemical structures and biological activities are stable, the storage and transportation are convenient, and it is not easy to deteriorate. In addition to direct crushing, the preparation method provided in the invention can reduce the dosage of the additive. It has the advantages of high bioavailability, quick effect, good absorption and good stability, and can be used for direct mixing feeding or drinking water feeding, and is convenient to use.

DESCRIPTION OF THE INVENTION

[0031] The invention is further described through specific embodiments.

[0032] EMBODIMENT 1

[0033] Raw material (Kg) shall be weighed according to the following weight ratios:

[0034] Herba Erodii seu Geranii 14 Herba Houttuyniae 23 Radix Sanguisorbae 20

[0035] Galla Chinensis 17 Radix Berberidis 20 Radix Glycyrrhizae 6

[0036] The preparation method is as follows:

[0037] (1) Herba Erodii seu Geranii, Radix Sanguisorbae, Galla Chinensis, Radix Berberidis, and Radix Glycyrrhizae were mixed and extracted 2 times by heating with % ethanol solution.

Each time for 2 hours, the extraction solid-liquid ratio was 8:1. Collect the extracting solution to recycle ethanol to get the mixed alcohol extractive, the alcohol extractive continued to decompress and concentrate, drying at 60 °C to get the mixed extractum, and crushed to 80 mesh micro-powder;

[0038] (2) Herba Houttuyniae was taken and extracted by water ultrasonic twice, each time for 30 minutes, the extraction solid-fluid ratio was 10:1. The extracting solution was collected and concentrated under reduced pressure. Herba Houttuyniae extractive dry extractum was obtained through microwave vacuum drying, and it was crushed to mesh micro-power.

[0039] (3) The raw materials obtained from the above two steps were mixed evenly and tested to be qualified for subpackage.

[0040] EMBODIMENT 2

[0041] Raw material (Kg) shall be weighed according to the following weight ratios:

[0042] Herba Erodii seu Geranii 14 Herba Houttuyniae 23 Radix Sanguisorbae 20

[0043] Galla Chinensis 17 Cortex Phellodendri 20 Radix Glycyrrhizae 6

[0044] The preparation method is as follows:

[0045] (1). Herba Erodii seu, Radix Sanguisorbae, Galla Chinensis, and Cortex Phellodendri were mixed and extracted 2 times by heating with 50% ethanol solution. Each time for 2 hours, the extraction solid-liquid ratio was 8:1. Collect the extracting solution to recycle ethanol to get the mixed alcohol extractive, the alcohol extractive continued to decompress and concentrate, spray drying to get the mixed extractum, and crushed to 80 mesh micro-powder;

[0046] (2) Herba Houttuyniae was taken and extracted by water flash 3 times, each time for 3 minutes, the extraction solid-fluid ratio was 12:1. The extracting solution was collected and concentrated under reduced pressure. Herba Houttuyniae extractive dry extractum was obtained through microwave vacuum drying, and it was crushed to 80 mesh micro-power;

[0047] (3) Radix Glycyrrhizae was crushed and dried to 80 mesh micro-power;

[0048] (4) The raw materials obtained from the above-mentioned 3 steps were mixed evenly and tested to be qualified for subpackage.

[0049] EMBODIMENT 3

[0050] Raw material (Kg) shall be weighed according to the following weight ratios:

Herba Erodii seu Geranii Herba Radix Sanguisorbae

[0051] 14 Houttuyniae 23 20

Radix Radix Glycyrrhizae

[0052] Galla Chinensis 17 Berberidis 20 6

[0053] The preparation method is as follows:

[0054] (1) Screening: Removing impurities from raw materials;

[0055] (2) Dosing: Raw materials were weighed according to the above ratio;

[0056] (3) Crushing: Raw materials which were tested qualified were crushed into fine powder;

[0057] (4) Mixing: Mix the crushed powder evenly according to the dosage;

[0058] (5) Tested to be qualified for subpackage.

[0059] EMBODIMENT 4

[0060] Raw material (Kg) shall be weighed according to the following weight ratios:

[0061] Herba Erodii seu Geranii Herba Houttuyniae 12 Radix Sanguisorbae 10 14

[0062] Galla Chinensis 12 Cortex Phellodendri 12 Radix Glycyrrhizae 4

[0063] Radix Scutellariae 4 Herba Polygoni Herba Portulacae 12 Perfoliati 6

[0064] Pileostegia viburnoides Cortex Acanthopanax Radicis (root, stem, leaf) Hook. f. et Thorns. 6 8

[0065] The preparation method is as follows:

[0066] (1) Herba Houttuyniae was taken according to the ratio and crushed into coarse powder. Extracting the volatile oil from, making the volatile oil into cyclodextrin inclusion complex, keeping the dregs of decoction in reserve;

[0067] (2) Herba Erodii seu Geranii, Radix Sanguisorbae, Galla Chinensis, Radix Berberidis, Radix Glycyrrhizae, Radix Scutellariae, Herba Polygoni Perfoliati, Pileostegia viburnoides Hook. f. et Thoms., Herba Portulacae, Cortex Acanthopanax Radicis (root, stem and/or leaf) and Cortex Phellodendri were crushed into coarse power, and together with the dregs of decoction obtained from step (1), were extracted with solvent respectively or in mixture. The extracting solution was collected, and the extractive was obtained by recycling the solvent;

[0068] (3) The extractive continued to be decompressed, concentrated and dried to obtain dry paste;

[0069] (4) The dry paste was crushed, and together with the cyclodextrin inclusion complex in step (1) was mixed evenly. Passing through 80 mesh sieve, it was tested to be qualified for subpackage, so that the finished product was obtained.

[0070] The beneficial effects of the invention are illustrated by clinical animal experiments and standardized farm feeding. The antibacterial, anti-inflammatory and antidiarrheal effects of the invention on the prevention and treatment of porcine diarrhea, enteritis, respiratory infection and other diseases based on pigs of different growing period as research objects, as well as its effects on the growth performance and immunity of fowl and livestock.

[0071] EXPERIMENTAL CASE 1 Effect on suckling sows and piglets

[0072] Taking suckling sows as the object of study, and through comparison with colistin sulfate and some disclosed inventions, the effects of the invention on weight gain, feed intake, survival rate, diarrheal index and blood biochemical indexes of suckling were investigated, further illustrating the beneficial effects of the invention on anti-bacterial and anti-diarrheal functions.

[0073] Materials and methods

[0074] 1. Experimental animals and grouping

[0075] Sows (Landrace xYorkshire) were divided into totaling 5 groups, each group was repeated 10 times for the experiment feeding of 50 piglets from 10 days after parturition and feeding experiment after parturition to 1 week after weaning. The experiment was designed as follows:(1) Normal control group (CON, basic diet), (2) positive control group (PC, basic diet +0.01% colistin sulfate (antibiotics)), (3) control group HEl (basic diet +2% Chinese herbal recipe 1 (CN101066313B, 2011.04.20)),(4) control group HE2 (basic diet +2% Chinese herbal recipe 2 (CN101584745A, 2009.11.25)), (5) experiment group HE (basic diet + 0.2% fodder additive of Embodiment 1 of the invention). The piglets were distributed uniformly at the amount of 10 to each repeated sows after parturition.

[0076] 2. Experiment feed and feeding management

[0077] In accordance with the requirements of the NRC (1998) [Nutrient requirements of domestic animals, NO. 2. Nutrient requisitions of swine. Ninth revised edition. National Academy of Science. Washington D.C.], the diet was based on corn - soybean meal. According to the feeding program given by suckling sows, it was given in the morning and evening restrictively and respectively, and the water could be freely acquired.

[0078] 3. Research indicators and methods

[0079] (1) Weight gain, feed intake, survival rate, diarrheal index (suckling sows). Weight gain were weighed and calculated at the beginning (the time of parturition) and the end, respectively. In terms of feed intake, when determining body weight, it was obtained by subtracting the residue from the given amount. In terms of survival rate, the total number of piglets determined daily from the beginning for calculation. In terms of diarrheal occurrence, it was determined on the day of the trial, 3, 6, 9 and 12 days, and 1 week after weaning.

[0080] (2) Biochemical indicators of blood (suckling sows)

[0081] In terms of the blood biochemical indexes of suckling piglets, any 3 were repeatedly selected at the end (at the time of weaning), and 2 mL of blood was collected from the jugular vein, and white blood cells (WBC), red blood cells (RBC), immunoglobulin IgG and lymphocytes were studied by automatic blood analyzer.

[0082] (3) Temperature of rectum, back waist and estrus after weaning of suckling sows

[0083] For all sows, the back waist thickness was measured at the beginning (parturition) and the end (at the time of weaning), and the back waist thickness at 3 positions of the midline 5 cm away from the 1" and 01 th ribs and the lumbar vertebra with digital backfat meter, and the 3 values were averaged; The temperature of the rectum, at the beginning (at the time of parturition) and at the end (at the time of weaning), was measured with a rectal thermometer; the days from the day of weaning to the day of estrus were calculated.

[0084] (4) Biochemical indexes of blood of suckling sows

[0085] The levels of relevant hormone in the blood of suckling sows were measured at the beginning (parturition) and the end (at the time of weaning). The blood was taken from all sows with blood collection tube at 2 mL, and centrifuged at 4 °C for 15 minutes at 3000 rpm. Cortisol, epinephrine and norepinephrine levels of the obtained serum were studied with the automatic biochemical analyzer.

[0086] (5) Nutrient digestibility rate (suckling sows)

[0087] In order to determine the digestibility rate of nutrients, excrement was taken at the end as the marking material, 0.2% Cr203 was added to the feed 5 days before the excrement was collected. The excrement was dried at 60 °C with hot air drier for 72 hours and then crushed. feed general component mixed with the marking substance Cr was analyzed according to AOAC standard.

[0088] 4. Statistical analysis

[0089] By using the normal linear model of SAS and using Duncan's new multiple range method, the mean value difference was obtained.

[0090] Results and discussion

[0091] 1. Production performance

[0092] When the feed is added that of the invention, the effect on the performance of suckling piglets is shown in Table 1.

[0093] For suckling piglets, the diarrhea index of CON group was significantly higher than that of HE1, HE2 and HE group (P <0.05), the diarrhea index at weaning period of CON group and HEl was significantly higher than that of HE2 and HE group (P <0.05). The invention has significant improvement in weight, daily weight gain, diet digestibility rate and survival rate.

[0094] Table 1 Effects of fodder additives on growth performance and anti-diarrheal function of piglets

[0095]

CON PC HEl HE2 HE SE

Initial weight/kg 1.32 1.45 1.34 1.42 1.57 0.11

Weaning weight/kg 5.64 6.37 6.21 6.35 6.59 0.38

Daily weight gain/g 206 234 232 235 239 15

Daily feed intake/g 105 115 96 104 89 18

Survival rate/% 92.96 97.72 95.63 95.42 97.23 3.10

Diarrhea rate at suckling9.75 5.00 2.75 3.25 2.25 3.30 period/%

Diarrheal rate at6.00 4.00 5.75 2.75 1.75 1.92 weaning period/%

Incidence of disease7.15 3.24 6.58 6.29 3.71 1.43 during the trial period

[0096] 2. There were no significant differences in blood biochemical indexes, rectum temperature, back waist and post-weaning estrus, nutrient digestibility in dry matter, nitrogen and energy digestion in suckling sows (P> 0.05).

[0097] Comprehensive analysis of the experimental results shows that the additive of the invention can enhance the body immunity of the sow, obviously promote the growth of the piglet, increase the daily weight gain, reduce the daily feed intake, greatly improve the feed efficiency, improve the survival rate, reduce the incidence of diarrheal occurrence during suckling and weaning period, and have obvious inhibitory effect on the occurrence of other diseases such as respiratory tract and pneumonia.

[0098] EXPERIMENTAL CASE 2: Effect on weaning piglets

[0099] Materials and methods

[0100] 1. Experimental animals and grouping

[0101] 250 ternary hybrid (Landrace xYorkshire xDuroc) weanling piglets were selected. At the beginning of the experiment, the weight was 10.03 0.5, the feeding experiment was carried out for 4 weeks. The experimental design was (1) The control group (CON, basic diet), (2) the positive control group (PC, basic diet +0.01% colistin sulfate (antibiotics)), (3) the control group HEl (basic diet +2% Chinese herbal recipe 1 (CN101066313B, 2011.04.20)), (4) control group HE2 (basic diet +2% Chinese herbal recipe 2 (CN101584745A, 2009.11.25)), (5) experiment group HE (basic diet +

0.2% fodder additive of the invention), totaling 5 groups. Each group was repeated 5 times, each containing 10 piglets.

[0102] 2. Experiment feed and feeding management

[0103] In the feed test, the feed was limited according to the standard, and the water could be drunk freely through the automatic feed machine.

[0104] 3. Research indicators and methods

[0105] (1) Weight gain method, feed intake and feed efficiency

[0106] In terms of weight gain, the weight of each group was measured at the beginning (14 days) and the end (28 days), respectively.

[0107] In terms of feed intake, when measuring body weight, it was obtained by subtracting the residue from the given feed amount.

[0108] In terms of feed efficiency, weight gain is divided by feed intake.

[0109] (2) Nutrient digestibility

[0110] In order to determine the digestibility rate of nutrients, excrement was taken at the experiment (14 days) and the end as the marking material, 0.2% Cr203 was added to the feed 5 days before the excrement was collected. The excrement was dried at 60 °C with hot air drier for 72 hours and then crushed. feed general component mixed with the marking substance Cr was analyzed according to AOAC standard.

[0111] (3) Biochemical indexes of blood

[0112] For blood collection, 6 pigs were randomly selected from each group, 2mL blood was taken from the jugular vein at the beginning and the end of the study, and white blood cells, red blood cells and IgG and lymphocytes were studied with the automatic blood analyzer. In addition, the serum biochemical examination was carried out at the beginning and the end of the experiment. The blood was taken with the blood collection tube, and then centrifuged at 5mL, 4 °C and 3000rpm for 30 minutes, and the serum was applied for analysis. The total protein and albumin in the serum were analyzed by Biuret method and BCG with the automatic biochemical analyzer.

[0113] (4) Statistical analysis (the same as Experiment Case 1)

[0114] II Results and discussion

[0115] 1 Production performance

[0116] When the feed is added that of the invention, the effect on the performance of weaning piglets is shown in Table 2. For piglets at the end of weaning, the weight of PC group and HE group was significantly higher than that of CON, HE1, and HE2 group (P <0.05), the daily weight gain of PC group was significantly higher than that of CON, HE l, and HE2 group. The comparison of HE group with CON, HE l and HE2 group has statistical meaning.

[0117] Table 2 Effect of adding different additives in feed on weaning piglet

[0118]

CON PC HEI HE2 HE SE Weaning weightkg 9.18 9.48 8.74 8.70 9.54 0.27 Wei ht at the end of the 23.04 27.79 22.57 22.73 26.17 0.62 test kg Daily weight gain 4 495 654 494 501 594 45 Daily intake g 716 733 711 721 704 11 Diarhealindex 33 19 27 19 20 6 Disease index 46 24 36 39 25 7

[0119] 2 Nutrient digestibility rate

[0120] There was no significant difference in nutrient digestibility of weaning piglets of dry matter, nitrogen and energy digestibility among groups when feed that prevented diarrheal occurrence was added (P> 0.05). PC and HE group was increased by about 3%.

[0121] 3 Blood biochemical index

[0122] There was no significant difference of blood biochemical indexes of weaning piglets in white blood cells, red blood cells, IgG and lymphocytes between groups when feed was added to prevent diarrheal occurrence (P> 0.05).

[0123] Therefore, by applying on the weaning piglets, the invention can increase daily weight gain, reduce feed intake, greatly improve feed efficiency, improve nutrient digestibility, prevent and reduce the occurrence of diseases such as diarrheal disease, and has a good effect of anti-bacterial, anti-inflammatory and anti-diarrheal functions.

[0124] EXPERIMENTAL CASE 3: Effect on growth ability of weaning piglets growing pigs

[0125] Materials and methods

[0126] 1. Experimental animals and grouping

[0127] 200 ternary hybrid (Landrace xYorkshire xDuroc) weanling piglets were selected as the experimental animals. At the beginning of the experiment, the average weight was 7 kg. The experiment was carried out at the ordinary farm for 10 weeks (piglet period 5 weeks + growth period 5 weeks).

[0128] Treatment group:(1) control group (CON, basic diet), (2), traditional Chinese medicine control group (HE, basic diet +0.5% Chinese medicine fodder additive (CN10106313B, 2011.04.20)), (3) experimental group HEl (basic diet +0.2% fodder additive of Embodiment 3 of the invention), (4) experimental group HE2 (basic diet +0.2% fodder additive of Embodiment 1 of the invention), (5) experimental group HE3 (basic diet +0.2% fodder additive of Embodiment 2 of the invention). Each group was repeated 2 times, each containing 20 piglets.

[0129] 2. Determining indicators and methods

[0130] (1) Productivity

[0131] Weight and feed intake were measured at the beginning, 5 weeks and the end (10 weeks), respectively, and their daily weight gain, daily feed intake and feed efficiency were calculated.

[0132] (2) Incidence of diarrheal occurrence

[0133] In terms of the incidence of diarrheal occurrence, the number of individuals with diarrheal occurrence was recorded with observation and judgement by anus massage from 1 stage feed (1 stage feed for weaning piglets) to 1-6 days after 2 stage feed (2 stage feed for weaning piglets), and 5 weeks after starting from 3 stage feed to 1-6 days after growing pig feed.

[0134] 3. Statistical processing

[0135] By using the normal linear model of SAS (1996) of all the data and using new multiple range method, the mean value difference was obtained.

[0136] II Results and discussion

[0137] 1. Production performance

[0138] When the piglet feed is added that of the invention, the effect on the production performance is shown in Table 3. Compared with control group and HE group, HEl, HE2 and HE3 increased significantly in weight on 5 th week, daily weight gain and feed efficiency(P <0.05). The daily feed intake of HE3 group, control group and HE group was significantly improved (P <0.05). At the end of the experiment (10 weeks), the weight, daily weight gain and feed intake of HE, HE2, HE3 group were significantly higher than that of control group and HE group (P <0.05). During the whole trial period, the daily weight gain of HEl, HE2, HE3 was higher than that of control group and HE group (P <0.05).

[0139] Table 3 Effect of adding the invention in piglet feed on growth ability

[0140]

CON HE HEl HE2 HE3 SE Initial weight/kg 7.17 7.34 7.21 7.33 7.19 0.13 Weight after 5 weeks/kg 24.26 24.82 28.29 27.10 29.20 1.65 Weightafter 10 weeksg46.94 47.25 53.25 52.36 54.33 2.14

0-5 weeks Daily weight gan/g 488 499 602 593 629 17 Daily intake/g 613 658 681 673 731 53 Feed eEciency 0.796 0.758 0.884 0.881 0.861 0.06 0-5 weeks Daily weight gainig 648 641 713 693 718 38 Daily intake/g 1.436 1.424 1.562 1.602 1.549 56 Feed efficiency 0.451 0.450 0.457 0.433 0.464 0.03

Whole trial period Daily weight gaing 568 570 658 643 673 25 Daly intake/g 1.025 1.041 1.122 1.138 1.140 51 Feed effciency 0.554 0.548 0.587 0.565 0.590 0.07

[0141] 2. Diarrheal index

[0142] The number of piglets with diarrheal occurrence when adding the invention to the piglet feed is shown in Table 4. They were fed at the parturition place after 4-5 days of weaning with No.1 feed (weaning pig 1 stage feed), and 2 stage feed after moving, No. 2 feed (weaning pig 2 stage feed), and 1 day later, 4-7 pigs of each treatment group showed diarrheal occurrence, but HE2 and HE3 showed no diarrheal occurrence from the 3 rd day from the starting of the experiment, while diarrheal occurrence of H group and HEl group stopped at the 4 thday of the experiment, and CON group at the 6th day. No. 3 feed (weaning pig 3 stage feed) was given at the 3 rd week of the experiment, and % of No. 3 feed together with 50% of growth pig feed (piglet feed) was provided at the 4th week. After the provision of the feed, 2-3 pigs of CON group and HE group showed diarrheal occurrence, which went stable after 1-2 days, while HE1, HE2, and HE3 groups showed no diarrheal occurrence after the replacement of feed.

[0143] Table 4 Effect of the invention on diarrheal occurrence of weaning piglets

[0144]

CON HE HEl HE2 HE3 I Day 5 6 7 4 5 2Day 7 2 2 1 1 3 Day 5 1 1 -

4 Day 3 Day I - 6 Day -

[0145] Table 5 Effect of the invention on diarrheal occurrence after replacement of growth pig feed

[0146]

CON HE IE1 HE2 HE3 I Day 3 2 -

2 Day 2 1 -

3 Day 1 1 -

4 Day -

5 Day -- 6 Day - - -

[0147] EXPERIMENTAL CASE 4: Influence on growth ability of fattening pigs

[0148] Research 1. Experimental animals and experiment design

[0149] 144 ternary hybrid (Landrace xYorkshire xDuroc) weanling piglets were selected as the experimental animals. At the beginning of the experiment, the average weight was 50.09 kg. The experiment was carried out at the farm for 10 weeks of feed experiment.

[0150] The treatment group was (1) CON (Diet), (2) A (Diet + the embodiment of the invention 10.1%), (3) B (Diet + the embodiment of the invention 20.1%), totaling 3 groups. Each group was repeated 12 times, each containing 4 piglets.

[0151] 2. Determining indicators and methods

[0152] Weight and feed intake were measured at the beginning (5 weeks) and the end (10 weeks), respectively, and their daily weight gain, daily feed intake and feed efficiency were calculated.

[0153] 3. Statistical processing

[0154] By using the normal linear model of SAS (1996) of all the data and using new multiple range method, the mean value difference was obtained.

[0155] II Results and discussion

[0156] 1. Production performance

[0157] When the feed of fattening pig is added the Chinese herbs of the invention, the effect on the production performance is shown in Table 6. In 0-5 weeks and the whole trial period, the daily increment of adding group (A and B) was significantly higher than that of CON group (P <0.05), and the daily increment in 5-10 weeks was significantly higher in group A than that of control (CON) group (P <0.05). There was no significant difference in daily feed intake and feed efficiency among each treatment group in 0-5 weeks, 5-10 weeks and the whole trial period.

[0158] Table 6 Effect of adding the invention to the feed on the performance of fattening

[0159]

CON A B SE 0-5 weeks

Daily weight gadng 751 837 819 12

Daily sitake/kg 1.972 2.107 2.075 0.058 Feed efficiency 0.381 0.397 0.395 0.01 0-5 weeks Daly weight gain/g 858 933 914 22 Daily stake/kg 2.372 2.564 2.531 79 Feedeffciency 0.362 0.365 0.361 0.01 Whole trial period

Daily weight gain 805 885 867 12

Daiy intake 2172 2336 2303 54 Feed efficiency 0.371 0.379 0.377 0.01

[0160] The addition of the invention can increase the daily weight gain and the daily feed intake of the fattening pig, and improve the feed efficiency to a certain extent. From the results of the experiment, the feed efficiency has not been improved significantly, while the increment of the daily weight gain and the daily feed intake is obvious. The conclusion shows that the mechanism of the invention that promotes the growth may be to prevent the disease of the body, improve the immunity of the body, and increase the palatability of the feed.

[0161] The above embodiments show and describe the basic principles, main features and advantages of the invention. The technical personnel of the industry shall understand that the invention is not limited by the above embodiments, that the foregoing embodiments and instructions describe only the principles of the invention, and that the scope of the invention is not limited by any means, and that, without breaking away from the scope of the invention, the invention will be subject to changes and improvements which shall all fall within the scope of the requested protection.

[162] Although the invention has been described with reference to specific examples, it will be appreciated by those skilled in the art that the invention may be embodied in many other forms, in keeping with the broad principles and the spirit of the invention described herein.

[163] The present invention and the described embodiments specifically include the best method known to the applicant of performing the invention. The present invention and the described preferred embodiments specifically include at least one feature that is industrially applicable

Claims

THE CLAIMS DEFINING THE INVENTION ARE AS FOLLOWS:

1. A natural fodder additive with antidiarrheal, antibacterial and anti inflammatory effects, which is composed of main drug and optional existing adjuvant composition, is characterized in that the main drug is composed of Herba Erodii seu Geranii, Herba Houttuyniae, Radix Sanguisorbae, Galla Chinensis, Radix Berberidis or Cortex Phellodendri, Radix Glycyrrhizae, Radix Scutellariae, Herba Polygoni Perfoliati, Pileostegia viburnoides Hook. f. et Thoms., Herba Portulacae, and Cortex Acanthopanax Radicis. Divided by weight, it contains 5~40 parts of Herba Erodii seu Geranii, 5~40 parts of Herba Houttuyniae, 2-15 parts of Radix Sanguisorbae, 2-35 parts of Galla Chinensis, 2~20 parts of Radix Berberidis or Cortex Phellodendri, 1-20 parts of Radix Glycyrrhizae, 0.5~10 parts of Radix Scutellariae, 1-8 parts of Herba Polygoni Perfoliati, 5~10 parts of Pileostegia viburnoides Hook. f. et Thoms., 2~30 parts of Herba Portulacae and 2-18 parts of Cortex Acanthopanax Radicis.

2. The fodder additive described in Claim 1 comprises the main drugs of: 10 parts of Herba Erodii seu Geranii, 12 parts of Herba Houttuyniae , 14 parts of Radix Sanguisorbae, 12 parts of Galla Chinensis, 12 parts of Cortex Phellodendri, 4 parts of Radix Glycyrrhizae, 4 parts of Radix Scutellariae, 6 parts of Herba Polygoni Perfoliati, 6 parts of Pileostegia viburnoides Hook. f. et Thoms., 12 parts of Herba Portulacae and 8 parts of Cortex Acanthopanax Radicis.

3. The fodder additive described in Claim lor 2 comprises 0-52 parts of adjuvant composition, which is one or more of wheat bran, rice bran, dextrin, starch and wheat middlings.

4. The use of fodder additive described in any of Claims 1-3 in the preparation of fowl and livestock feed with antidiarrheal, antibacterial or anti-inflammatory effects.

5. According to the purpose described in Claim 4, the fowl and livestock fodder is used for the prevention or treatment of respiratory or intestinal diseases of domestic fowl and livestock.

6. According to the purpose described in Claim 5, the disease described therein is pneumonia or dysentery.

7. The method for preparing the natural fodder additive described in any of Claims 1-3 is characterized in that:

a. Herba Erodii seu Geranii, Radix Sanguisorbae, Galla Chinensis, Radix Berberidis, Radix Glycyrrhizae, Radix Scutellariae, Herba Polygoni Perfoliati, Pileostegia viburnoides Hook. f. et Thoms., Herba Portulacae, Cortex Phellodendri and Cortex Acanthopanax Radicis are extracted with solvent respectively or in mixture. The extracting solution is collected, and the extractive is obtained by removing the solvent;

b. Herba Houttuyniae is extracted with water flash method. The extracting solution is collected, and the extractive is obtained by removing the solvent;

c. The extractive obtained from step a and b is dried into the dry paste, and then crushed and optionally added the adjuvant composition followed by uniform mixing.

8. The method for preparing the natural fodder additive described in any of Claims 1-3 is characterized by: a. extracting the volatile oil from Herba Houttuyniae, making the volatile oil into cyclodextrin inclusion complex, keeping the dregs of decoction in reserve; b. Herba Erodii seu Geranii, Radix Sanguisorbae, Galla Chinensis, Radix Berberidis, Radix Glycyrrhizae, Radix Scutellariae, Herba Polygoni Perfoliati, Pileostegia viburnoides Hook. f. et Thoms., Herba Portulacae, Cortex Phellodendri and Cortex Acanthopanax Radicis, together with the dregs of decoction obtained from step a, are extracted with solvent respectively or in mixture. The extracting solution is collected, and the extractive is obtained by removing the solvent;

c. The extractive obtained from step b is dried into the dry paste, and then crushed and optionally added the adjuvant composition and cyclodextrin inclusion complex obtained in step a followed by uniform mixing.

9. The method for preparing the natural fodder additive described in any of Claims 1-3 is characterized in that the main drug materials are mixed separately or in mixture, and then crushed and optionally added the adjuvant composition followed by uniform mixing.