CN115137020A - Anti-inflammatory feed composition for ruminants and preparation method thereof - Google Patents
Anti-inflammatory feed composition for ruminants and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
- A23K30/10—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D1/00—Evaporating
- B01D1/22—Evaporating by bringing a thin layer of the liquid into contact with a heated surface
- B01D1/222—In rotating vessels; vessels with movable parts
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
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- B01D11/02—Solvent extraction of solids
- B01D11/0288—Applications, solvents
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/0292—Treatment of the solvent
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
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- Health & Medical Sciences (AREA)
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Abstract
The invention discloses an anti-inflammatory feed composition for ruminants and a preparation method thereof, wherein the anti-inflammatory feed composition comprises the following components in parts by weight: 80-100 parts of whole ramie silage; 30-50 parts of purslane whole-plant micro powder; 10-15 parts of plant cake; 8-15 parts of compound anti-inflammatory active plant extract. The invention belongs to the field of ruminant feed compositions, and particularly relates to an anti-inflammatory feed composition for ruminants and a preparation method thereof; the plant extracts rich in various organic acidic active ingredients are used for replacing antibiotics, and a synergistic interaction effect is formed among the plant extracts, so that the dual technical effects of antagonizing pathogenic microorganisms to achieve the anti-inflammatory effect and reducing the damage to beneficial microorganisms are achieved, the problem that the prior art is difficult to solve, namely the problem that inflammation needs to be reduced and the problem that the digestion is difficult needs to be avoided is solved, and the anti-inflammatory feed composition for the ruminants, provided by the invention, has no residue, is safe and environment-friendly, and has an important application value.
Description
Technical Field
The invention belongs to the technical field of ruminant feed compositions, and particularly relates to an anti-inflammatory feed composition for ruminants and a preparation method thereof.
Background
The antibiotic is a substance which is obtained by microbial culture or chemical synthesis and has the function of specifically killing microorganisms, mainly comprises a feed antibiotic and a therapeutic antibiotic, the feed antibiotic is added into a premix by a certain technical means and plays a role in epidemic disease prevention and growth promotion in production, the feed antibiotic plays an important contribution in the development process of feed industry of China for 30 years, can play an immune role on ruminants, and effectively reduces diseases such as mastitis, metritis, rumen bloating and the like.
However, in recent years, the emergence of antibiotic residues and superbacteria in animal food has caused concern and apprehension about feed safety and food safety problems worldwide, and long-term use of feed antibiotics can destroy the steady state of microorganisms in the digestive tract, improve the drug resistance of antibiotics of bacterial pathogens and reduce the immunity of organisms, but the antibiotics are remained in ruminant products (meat and milk) and harm human health, so that the antibiotic is announced by the ministry of agricultural rural areas and is prohibited from being used in feed comprehensively in China to 2020, and the search for effective antibiotic substitutes which do not threaten public health is urgent for ensuring food safety and production benefit.
The natural plant resources in China are rich, the pharmacological activity is various, the plant extract has a plurality of health care effects of bacteriostasis, antitoxic property enhancement, stress capability improvement and the like, so the plant extract can be applied to the preparation of feed, for example, patent number 201910864954.X discloses a natural plant extract for a feed additive for poultry, the composite anti-inflammatory active plant extract composition is prepared from a sabina chinensis ethanol extract, a Chinese actinidia root water extract, an allium macrostemon cacumen extract, allium macrostemon volatile oil and cacumen biotae volatile oil according to the proportion of 5-9.
Unlike the animal with hindgut digestion, the rumen of the ruminant forms a unique digestion mode in the long-term selective evolution, can efficiently utilize structural carbohydrates such as cellulose and hemicellulose to generate nutrients required by the body, and is important for reducing the use of antibiotics by developing plant extracts applicable to the ruminant, but the main problems of the prior art are that: the single plant extract has low effect and cannot be compared with antibiotics, so the single plant extract has no applicability; the added plant extract is too acidic, so that the vitamin in the feed is lost; the active ingredients in the plant extract kill beneficial microorganisms while quickly killing pathogenic microorganisms in rumen, so that the decomposition of high cellulose substances is not facilitated, and in addition, the antibiotic has selectivity on the antagonism of the microorganisms, so that the application range of the antibiotic is limited.
Disclosure of Invention
Aiming at the situation and overcoming the defects of the prior art, the invention realizes the dual technical effects of realizing the antagonistic action of pathogenic microorganisms to realize the anti-inflammatory effect and reducing the damage to beneficial microorganisms by using plant extracts rich in various organic acid active ingredients to replace antibiotics according to the plant species of homology of medicine and food specified by the national ministry of health and forming the synergistic effect among the plant extracts, and solves the contradiction problem which is difficult to solve by the prior art that the anti-inflammatory effect is realized (the inflammation is reduced) and the anti-bacterial effect is not realized (the digestion difficulty is avoided); meanwhile, the plant extract containing the alkaloid active substances reduces the absorption of organic acid active ingredients in the plant extract to vitamins before the feed is ingested, so that the loss of the vitamins is reduced; the anti-inflammatory feed composition for the ruminants, provided by the invention, has the characteristics of no residue, no public nuisance, safety and environmental protection, has important economic significance and social benefit for developing green animal husbandry in China, reducing the gap with developed countries, meeting the requirements of people on food safety and improving the international competitiveness of products.
In order to achieve the above object, the present invention provides an anti-inflammatory feed composition for ruminants, comprising the following components in parts by weight:
80-100 parts of whole ramie silage;
30-50 parts of purslane whole-plant micro powder;
10-15 parts of plant cake;
8-15 parts of compound anti-inflammatory active plant extract.
Preferably, the plant cake comprises one or more of flaxseed cake, rapeseed cake, and soybean cake.
Further, in order to improve the anti-inflammatory and anti-inflammatory capability of ruminants, the composite anti-inflammatory active plant extract comprises the following components in parts by weight:
3-9 parts of honeysuckle crude extract;
2-8 parts of a licorice crude extract;
2-5 parts of oroxylum indicum extract;
1-5 parts of dandelion extract;
1-4 parts of dysentery stopping grass extract.
Preferably, the composite anti-inflammatory active plant extract comprises the following components in parts by weight:
5-9 parts of honeysuckle crude extract;
4-8 parts of a licorice crude extract;
3-5 parts of oroxylum indicum extract;
2-5 parts of dandelion extract;
1-2 parts of an antidiarrheal extract.
The invention also provides a preparation method of the anti-inflammatory feed composition for the ruminants, which comprises a preparation method of a honeysuckle crude extract, a preparation method of a licorice crude extract, a preparation method of a oroxylum indicum extract, a preparation method of a dandelion extract and a preparation method of an anti-dysentery grass extract.
Preferably, the preparation method of the honeysuckle crude extract comprises the following steps:
(1) Removing impurities from flos Lonicerae raw material, grinding, and sieving with 50 mesh and 100 mesh sieve to obtain flos Lonicerae fine powder;
(2) Adding the honeysuckle fine powder into the mixture according to the volume ratio of 1: refluxing with 10 times of water for 2-3 hr, filtering, and collecting primary filtrate and residue of flos Lonicerae;
(3) Adding the filter residue obtained in the primary honeysuckle coarse extraction into the mixture again, wherein the volume ratio of the filter residue is 1: continuously refluxing with 5 times of water for 1-2 hours, filtering, collecting crude extract of flos Lonicerae, and collecting secondary filtrate;
(4) Mixing the primary honeysuckle crude extraction filtrate and the secondary honeysuckle crude extraction filtrate, concentrating by using a reduced pressure rotary evaporator and recovering the solvent.
Preferably, the preparation method of the glycyrrhiza coarse extract comprises the following steps:
(1) Removing impurities from Glycyrrhrizae radix, grinding, and sieving with 50-mesh and 100-mesh sieve to obtain Glycyrrhrizae radix fine powder;
(2) Adding the liquorice fine powder into the mixture according to the volume ratio of 1: refluxing 8 times of water for 2-3 hours, filtering and collecting primary filtrate of crude extraction of liquorice and primary filter residue of crude extraction of liquorice;
(3) And adding the filter residue obtained in the primary licorice crude extraction into the mixture again, wherein the volume ratio of the filter residue is 1: refluxing 4 times of water for 1-2 hr, filtering, and collecting the secondary filtrate;
(4) Mixing the primary licorice crude extraction filtrate and the secondary licorice crude extraction filtrate, concentrating by using a reduced pressure rotary evaporator, and recovering the solvent.
As a further preferable aspect of the present invention, the preparation method of the oroxylum indicum extract comprises the following steps:
(1) Removing impurities from semen Oroxyli raw material, grinding, and filtering with 50-mesh and 100-mesh sieve to obtain semen Oroxyli fine powder;
(2) Adding the oroxylum indicum fine powder into the mixture according to the volume ratio of 1: heating and refluxing 10 times of 75% ethanol for 3 hours, filtering and collecting filtrate and filter residue of oroxylum indicum extract;
(3) Adding the filtrate obtained by the primary oroxylum indicum extraction into the filtrate again in a volume ratio of 1: heating and refluxing 5 times of 75% ethanol for 2 hours, and collecting oroxylum indicum extract secondary filtrate;
(4) Mixing the first oroxylum indicum extraction filtrate and the second oroxylum indicum extraction filtrate, concentrating at the temperature of below 35-40 ℃ by using a reduced pressure rotary evaporator, and recovering the solvent.
Preferably, the preparation method of the dandelion extract comprises the following steps:
(1) Removing impurities from herba Taraxaci raw material, grinding, and filtering with 50 mesh and 100 mesh sieve to obtain herba Taraxaci fine powder;
(2) Adding the dandelion fine powder into a mixture with a volume ratio of 1: heating and refluxing 8 times of 75% ethanol for 2 hr, filtering, and collecting filtrate and residue;
(3) Adding the filtrate obtained by the first dandelion extraction into the filtrate again in a volume ratio of 1: heating and refluxing 5 times of 75% ethanol for 1 hr, and collecting herba Taraxaci extract secondary filtrate;
(4) Mixing the first and second herba Taraxaci extracts, concentrating at 35-40 deg.C with reduced pressure rotary evaporator, and recovering solvent.
Preferably, the preparation method of the dysentery stopping grass extract comprises the following steps:
(1) Removing impurities from the raw materials of the dysentery stopping grass, grinding, and filtering by using a sieve of 50 meshes and a sieve of 100 meshes to obtain fine powder of the dysentery stopping grass;
(2) Adding the fine powder of the dysentery stopping grass into the mixture according to the volume ratio of 1: heating 10 times of 75% ethanol at a volume ratio, refluxing for 2 hr, filtering, collecting filtrate and residue;
(3) Adding the filtrate obtained by extracting the dysentery stopping grass into the filtrate again according to the volume ratio of 1: heating and refluxing 5 times of 75% ethanol for 1 hr, collecting the filtrate, and extracting with herba Lespedezae Buergeri to obtain secondary filtrate;
(4) Mixing the first filtrate and the second filtrate, concentrating at 35-40 deg.C with reduced pressure rotary evaporator, and recovering solvent.
The invention with the structure has the following beneficial effects:
(1) According to the invention, according to the plant species of homology of medicine and food specified by the national ministry of health, by using the plant extract rich in various organic acidic active ingredients to replace antibiotics, the dual technical effects of carrying out antagonism on pathogenic microorganisms to realize anti-inflammatory efficacy and reducing damage to beneficial microorganisms are realized, and the contradiction problem that the prior art is difficult to solve, namely the contradiction problem that the prior art needs antibiosis (reduces inflammation) and cannot (avoids difficult digestion) is solved;
(2) The effects of different plant species are matched to form an interaction relation, and the synergistic interaction effect is formed among the active compositions, so that the anti-inflammatory feed composition with the characteristics of no residue, no public nuisance, safety and environmental protection is formed;
(3) The honeysuckle crude extract contains chlorogenic acid as a main active ingredient, and the liquorice crude extract contains glycyrrhizic acid as a main active ingredient, wherein the chlorogenic acid and the glycyrrhizic acid have the effects of resisting bacteria and enhancing the immunity of the organism and have an inhibiting effect on various pathogenic bacteria;
(4) The purslane whole plant micro powder has the functions of clearing away heat and toxic materials, removing dust, sterilizing, diminishing inflammation and relieving pain;
(5) The active alkaloid components in the oroxylum indicum extract and the dandelion extract and organic acid form organic acid salts, and the organic acid salts can promote the absorption of nutrients while slowly releasing in the rumen of the ruminant;
(6) The plant extract containing the alkaloid active substances reduces the absorption of the organic acid active ingredients of the plant extract to vitamins before the feed is ingested, thereby reducing the loss of the vitamins.
Drawings
FIG. 1 is a diagram showing the inhibitory effect of a composite anti-inflammatory active plant extract in an anti-inflammatory feed composition on pathogenic bacteria of ruminant inflammation.
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments; all other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
In the examples of the present invention, unless otherwise specified, it is understood that the raw materials and the treatment techniques are all conventional and commercially available raw materials and conventional treatment techniques in the art.
Example 1
The preparation method of the compound anti-inflammatory active plant extract comprises a preparation method of a honeysuckle crude extract, a preparation method of a liquorice crude extract, a preparation method of a oroxylum indicum extract, a preparation method of a dandelion extract and a preparation method of a dysentery stopping grass extract.
The preparation method of the honeysuckle crude extract comprises the following steps:
(1) Removing impurities from flos Lonicerae raw material, grinding, and sieving with 50 mesh and 100 mesh sieve to obtain flos Lonicerae fine powder;
(2) Adding the honeysuckle fine powder into the mixture according to the volume ratio of 1:10 times of the amount of water, and the like, refluxing for 2-3 hr, filtering, and collecting primary filtrate and residue of flos Lonicerae;
(3) Adding the mixture into the filter residue obtained in the primary honeysuckle coarse extraction in a volume ratio of 1: continuously refluxing with 5 times of water for 1-2 hours, filtering, collecting crude extract of flos Lonicerae, and collecting secondary filtrate;
(4) Mixing the primary filtrate obtained by the crude extraction of the honeysuckle and the secondary filtrate obtained by the crude extraction of the honeysuckle, concentrating by using a reduced pressure rotary evaporator and recovering the solvent.
The preparation method of the liquorice crude extract comprises the following steps:
(1) Removing impurities from Glycyrrhrizae radix, grinding, and sieving with 50-mesh and 100-mesh sieves to obtain Glycyrrhrizae radix fine powder;
(2) Adding the liquorice fine powder into the mixture according to the volume ratio of 1: refluxing 8 times of water for 2-3 hours, filtering and collecting primary filtrate of crude extraction of liquorice and primary filter residue of crude extraction of liquorice;
(3) Adding the filter residue obtained after the primary extraction of the liquorice into the mixture again, wherein the volume ratio of the filter residue is 1: refluxing 4 times of water for 1-2 hr, filtering, and collecting the secondary filtrate;
(4) Mixing the primary filtrate obtained by the crude extraction of the liquorice and the secondary filtrate obtained by the crude extraction of the liquorice, concentrating by using a reduced pressure rotary evaporator and recovering the solvent.
In addition, the preparation method of the oroxylum indicum extract comprises the following steps:
(1) Removing impurities from semen Oroxyli raw material, grinding, and filtering with 50 mesh and 100 mesh sieves to obtain semen Oroxyli fine powder;
(2) Adding the oroxylum indicum fine powder into the mixture according to the volume ratio of 1: heating and refluxing 10 times of 75% ethanol for 3 hours, filtering and collecting oroxylum indicum primary extraction filtrate and oroxylum indicum primary extraction filter residue;
(3) Adding the filtrate obtained by the primary extraction of the oroxylum indicum into the mixture again in a volume ratio of 1: heating and refluxing 5 times of 75% ethanol for 2 hours, and collecting oroxylum indicum extract secondary filtrate;
(4) Mixing the primary filtrate and the secondary filtrate, concentrating at 35-40 deg.C with reduced pressure rotary evaporator, and recovering solvent.
The preparation method of the dandelion extract comprises the following steps:
(1) Removing impurities from herba Taraxaci raw material, grinding, and filtering with 50 mesh and 100 mesh sieve to obtain herba Taraxaci fine powder;
(2) Adding the dandelion fine powder into the mixture according to the volume ratio of 1: heating and refluxing 8 times of 75% ethanol for 2 hr, filtering, and collecting filtrate and residue;
(3) Adding the mixture into the filtrate obtained by the first dandelion extraction again, wherein the volume ratio of the mixture is 1: heating and refluxing 5 times of 75% ethanol for 1 hr, and collecting herba Taraxaci extract secondary filtrate;
(4) Mixing the first and second filtrates, concentrating at 35-40 deg.C under reduced pressure, and recovering solvent.
The preparation method of the dysentery stopping grass extract comprises the following steps:
(1) Removing impurities from the raw materials of the dysentery stopping grass, grinding, and filtering by using a sieve of 50 meshes and a sieve of 100 meshes to obtain fine powder of the dysentery stopping grass;
(2) Adding the fine powder of the dysentery stopping grass into the mixture according to the volume ratio of 1: heating 10 times of 75% ethanol at a volume ratio, refluxing for 2 hr, filtering, collecting filtrate and residue;
(3) Adding the filtrate obtained by extracting the dysentery stopping grass for the first time into the filtrate again according to the volume ratio of 1: heating and refluxing 5 times of 75% ethanol for 1 hr, collecting the secondary filtrate of herba Centellae extract;
(4) Mixing the first filtrate and the second filtrate, concentrating at 35-40 deg.C under reduced pressure with rotary evaporator, and recovering solvent.
Mixing the prepared honeysuckle crude extract, liquorice crude extract, oroxylum indicum extract, dandelion extract and dysentery stopping grass extract to prepare the composite anti-inflammatory active plant extract.
Example 2
Preparation of anti-inflammatory feed composition for ruminants
The invention provides an anti-inflammatory feed composition for ruminants, which comprises the following components in parts by weight:
ensiling the whole ramie by 80 parts;
30 parts of purslane whole-plant micro powder;
15 parts of plant cake;
10 parts of compound anti-inflammatory active plant extract.
Wherein the plant cake is semen Lini cake.
In addition, the composite anti-inflammatory active plant extract comprises the following components in parts by weight:
9 parts of honeysuckle crude extract;
7 parts of licorice crude extract;
4 parts of oroxylum indicum extract;
3 parts of dandelion extract;
1 part of dysentery stopping grass extract.
The components are mixed evenly to obtain the anti-inflammatory feed composition for the ruminants.
Example 3
Preparation of anti-inflammatory feed composition for ruminants
The invention provides an anti-inflammatory feed composition for ruminants, which comprises the following components in parts by weight:
ensiling the whole ramie by 80 parts;
30 parts of purslane whole-plant micro powder;
15 parts of plant cake;
10 parts of compound anti-inflammatory active plant extract.
Wherein the plant cake is linseed cake.
The composite anti-inflammatory active plant extract comprises the following components in parts by weight:
8 parts of honeysuckle crude extract;
6 parts of licorice crude extract;
3 parts of oroxylum indicum extract;
4 parts of dandelion extract;
2 parts of dysentery stopping grass extract.
The anti-inflammatory feed composition for the ruminant is obtained after the components are mixed uniformly.
Example 4
Preparation of anti-inflammatory feed composition for ruminants
The invention provides an anti-inflammatory feed composition for ruminants, which comprises the following components in parts by weight:
ensiling the whole ramie by 80 parts;
30 parts of purslane whole-plant micro powder;
15 parts of plant cake;
10 parts of compound anti-inflammatory active plant extract.
Wherein the plant cake is linseed cake.
The composite anti-inflammatory active plant extract comprises the following components in parts by weight:
7 parts of honeysuckle crude extract;
8 parts of a licorice crude extract;
3 parts of oroxylum indicum extract;
2 parts of dandelion extract;
1 part of dysentery stopping grass extract.
The components are mixed evenly to obtain the anti-inflammatory feed composition for the ruminants.
Comparative example 1
This comparative example provides an anti-inflammatory feed composition for ruminants, which is different from example 2 only in that the composite anti-inflammatory active plant extract in the components is 50% of enemin, and the remaining components, the content of the components are the same as in example 2, and the preparation method of the feed composition is as in example 2.
Comparative example 2
This comparative example provides an anti-inflammatory feed composition for ruminants, which is different from example 2 only in that the composite anti-inflammatory active plant extract in the components is 50% kitasamycin, and the contents of the remaining components and components are the same as example 2, and the preparation method of the feed composition is as in example 2.
Example 5
In-vitro antibacterial activity determination of composite anti-inflammatory active plant extract on ruminant inflammation pathogenic bacteria
Escherichia bovis enters the digestive tract through the oral cavity to cause intestinal inflammation and diarrhea in ruminants, and Salmonella enteritidis causes diarrhea by producing enterotoxin, so that Escherichia bovis and Salmonella enteritidis are used as experimental subjects for determining the inhibitory effect of the natural extract on pathogenic bacteria in the present invention, and in addition, bacillus subtilis produces cellulose-degrading enzymes in the rumen of ruminants, is a microorganism which is beneficial to the degradation of food of ruminants, so the research is carried out by using bacillus subtilis as a control, wherein, the escherichia bovis (CVCC 215) is purchased from China veterinary microbial culture collection management center, the salmonella inflammation causing bacteria (CICC 21503) is purchased from China industrial microbial culture collection management center, and the bacillus subtilis (CICC 20522) is purchased from China industrial microbial culture collection management center.
The used Oxford cup and glass were first culturedSterilizing the dishes at 121 deg.C for 15-20 min, vertically placing Oxford cup in a sterile operating table with sterilized clamp, preparing MHA culture medium (company name: solarbio; product number: M8550) according to the instruction, pouring into glass culture dish, cooling to 35-40 deg.C, adding test bacteria solution, mixing completely to obtain bacteria solution with concentration of 1.0 × 10 6 CFU/mL; then after the medium solidified, the oxford cup was pulled out and 200 μ L of the complex anti-inflammatory active plant extract solution prepared in example 2, example 3, example 4, comparative example 1 and comparative example 2 was added, and escherichia coli was treated with sterile water as a blank, with 3 replicates in a single treatment setup and 3 replicates in the experiment; sealing the culture medium after the plant extract solution is completely air-dried, placing the culture medium in a constant temperature incubator at 37 ℃ for 24 h, and measuring the diameter (mm) of the inhibition zone.
TABLE 1 size of in vitro inhibition zone of complex anti-inflammatory active plant extracts against pathogenic bacteria of ruminant inflammation
As shown in FIG. 1, A, B and C show inhibition zones generated by treating Escherichia coli, salmonella enteritidis and Bacillus subtilis with the composite anti-inflammatory active plant extract of example 2, and D shows inhibition zones generated by treating Escherichia coli with sterile water, and it can be seen from Table 1 that the composite anti-inflammatory active plant extracts prepared in examples 2, 3 and 4 all have an inhibitory effect on pathogenic bacteria causing inflammation in ruminants, but have partial inhibition on the beneficial bacteria, but are significantly smaller than common antibiotics, so that the situation that the microbial environment inside the ruminants is damaged due to long-term use of antibiotics and the self-growth and development are influenced is avoided.
Example 6
The anti-inflammatory feed composition for the ruminants prepared by the invention is used for feeding experiments of cattle
300 grams of the feed compositions prepared in example 2, example 3, example 4, comparative example 1 and comparative example 2 were mixed with one ton of basic ration and fed to ruminants (beef cattle were used as experimental subjects) with a concentrate feed as a blank control, wherein the concentrate feed was fed in an amount of 0.80% of the weight of beef cattle, the daily average weight gain of beef cattle was measured for 7 days and 14 days and the diarrhea rate was calculated as a percentage of the number of beef cattle with diarrhea for 7 days and 14 days, based on the number of all beef cattle tested.
TABLE 2 anti-inflammatory feed composition for ruminants feeding experimental results
As shown in Table 2, after the anti-inflammatory feed composition for the ruminants prepared by the invention is fed, the diarrhea rate of the ruminants is reduced and the weight of the ruminants is increased due to inflammation, and the anti-inflammatory feed composition for the ruminants provided by the invention has the characteristics of no residue, no public hazard, safety and environmental protection, has important economic significance and social benefits for developing green animal husbandry, narrowing the gap with developed countries, meeting the requirements of people on food safety and improving the international competitiveness of products in China.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
The present invention and its embodiments have been described above, and the description is not intended to be limiting, and what is shown in the drawings is only one embodiment of the present invention, and the practical application is not limited thereto. In summary, those skilled in the art should appreciate that combinations and embodiments similar to those of the above-described embodiments can be devised without departing from the spirit of the invention and the scope of the invention.
Claims (10)
1. An anti-inflammatory feed composition for ruminants, characterized in that: the anti-inflammatory feed composition comprises the following components in parts by weight:
80-100 parts of whole ramie silage;
30-50 parts of purslane whole-plant micro powder;
10-15 parts of plant cake;
8-15 parts of compound anti-inflammatory active plant extract.
2. The anti-inflammatory feed composition for ruminants as claimed in claim 1, wherein: the composite anti-inflammatory active plant extract comprises the following components in parts by weight:
3-9 parts of honeysuckle crude extract;
2-8 parts of a licorice crude extract;
2-5 parts of oroxylum indicum extract;
1-5 parts of dandelion extract;
1-4 parts of dysentery stopping grass extract.
3. The anti-inflammatory feed composition for ruminants as claimed in claim 2, wherein: the composite anti-inflammatory active plant extract comprises the following components in parts by weight:
5-9 parts of honeysuckle crude extract;
4-8 parts of a licorice crude extract;
3-5 parts of oroxylum indicum extract;
2-5 parts of dandelion extract;
1-2 parts of an antidiarrheal extract.
4. The anti-inflammatory feed composition for ruminants as claimed in claim 3, wherein:
the plant cake comprises one or more of linseed cake, rapeseed cake and soybean cake.
5. A method for preparing an anti-inflammatory feed composition for ruminants according to claim 1, wherein: the preparation method comprises a honeysuckle crude extract preparation method, a licorice crude extract preparation method, an oroxylum indicum extract preparation method, a dandelion extract preparation method and an anti-dysentery grass extract preparation method, the honeysuckle crude extract, the licorice crude extract, the oroxylum indicum extract, the dandelion extract and the anti-dysentery grass extract are mixed to prepare a composite anti-inflammatory active plant extract, and the composite anti-inflammatory active plant extract is mixed with the whole ramie silage, the purslane whole grass micro powder and the plant cake to prepare the anti-inflammatory feed composition.
6. The method of claim 5, wherein the feed composition is prepared by mixing the following ingredients: the preparation method of the honeysuckle crude extract comprises the following steps:
(1) Removing impurities from flos Lonicerae raw material, grinding, and sieving with 50 mesh and 100 mesh sieve to obtain flos Lonicerae fine powder;
(2) Adding the honeysuckle fine powder into the mixture according to the volume ratio of 1: refluxing with 10 times of water for 2-3 hr, filtering, and collecting primary filtrate and residue of flos Lonicerae;
(3) Adding the filter residue obtained in the primary honeysuckle coarse extraction into the mixture again, wherein the volume ratio of the filter residue is 1: continuously refluxing with 5 times of water for 1-2 hours, filtering, collecting crude extract of flos Lonicerae, and collecting secondary filtrate;
(4) Mixing the primary honeysuckle crude extraction filtrate and the secondary honeysuckle crude extraction filtrate, concentrating by using a reduced pressure rotary evaporator and recovering the solvent.
7. The method of claim 6, wherein the feed composition is prepared by mixing the following ingredients: the preparation method of the licorice crude extract comprises the following steps:
(1) Removing impurities from Glycyrrhrizae radix, grinding, and sieving with 50-mesh and 100-mesh sieve to obtain Glycyrrhrizae radix fine powder;
(2) Adding the liquorice fine powder into the mixture according to the volume ratio of 1: refluxing 8 times of water for 2-3 hours, filtering and collecting primary filtrate of crude extraction of liquorice and primary filter residue of crude extraction of liquorice;
(3) And adding the filter residue obtained after the primary extraction of the liquorice into the mixture again, wherein the volume ratio of the filter residue to the primary extraction of the liquorice is 1: refluxing 4 times of water for 1-2 hr, filtering, and collecting the secondary filtrate;
(4) Mixing the primary licorice crude extraction filtrate and the secondary licorice crude extraction filtrate, concentrating by using a reduced pressure rotary evaporator, and recovering the solvent.
8. The method of claim 7, wherein the feed composition is prepared by mixing the following ingredients: the preparation method of the oroxylum indicum extract comprises the following steps:
(1) Removing impurities from semen Oroxyli raw material, grinding, and filtering with 50-mesh and 100-mesh sieve to obtain semen Oroxyli fine powder;
(2) Adding the oroxylum indicum fine powder into the mixture according to the volume ratio of 1: heating and refluxing 10 times of 75% ethanol for 3 hours, filtering and collecting oroxylum indicum primary extraction filtrate and oroxylum indicum primary extraction filter residue;
(3) Adding the filtrate obtained by the primary oroxylum indicum extraction into the filtrate again in a volume ratio of 1: heating and refluxing 5 times of 75% ethanol for 2 hours, and collecting oroxylum indicum extract secondary filtrate;
(4) And mixing the primary oroxylum indicum extraction filtrate and the secondary oroxylum indicum extraction filtrate, concentrating by using a reduced pressure rotary evaporator, and recovering the solvent.
9. The method of claim 8, wherein the feed composition is prepared by mixing the following ingredients: the preparation method of the dandelion extract comprises the following steps:
(1) Removing impurities from herba Taraxaci raw material, grinding, and filtering with 50 mesh and 100 mesh sieve to obtain herba Taraxaci fine powder;
(2) Adding the dandelion fine powder into a mixture with a volume ratio of 1: heating and refluxing 8 times of 75% ethanol for 2 hours, filtering, collecting the first filtrate and the first residue of herba Taraxaci extraction;
(3) Adding the filtrate obtained by the first dandelion extraction into the filtrate again in a volume ratio of 1: heating and refluxing 5 times of 75% ethanol for 1 hr, collecting herba Taraxaci extract and filtering to obtain secondary filtrate;
(4) Mixing the first herba Taraxaci extracting filtrate and the second herba Taraxaci extracting filtrate, concentrating with a reduced pressure rotary evaporator, and recovering solvent.
10. The method of claim 9, wherein the feed composition is prepared by mixing the following ingredients: the preparation method of the dysentery stopping grass extract comprises the following steps:
(1) Removing impurities from the raw materials of the dysentery stopping grass, grinding, and filtering by using a sieve of 50 meshes and a sieve of 100 meshes to obtain fine powder of the dysentery stopping grass;
(2) Adding the fine powder of the dysentery stopping grass into the mixture according to the volume ratio of 1: heating 10 times of 75% ethanol at a volume ratio, refluxing for 2 hr, filtering, collecting filtrate and residue;
(3) Adding the filtrate obtained by extracting the dysentery stopping grass into the filtrate again according to the volume ratio of 1: heating and refluxing 5 times of 75% ethanol for 1 hr, collecting the filtrate, and extracting with herba Lespedezae Buergeri to obtain secondary filtrate;
(4) And mixing the primary extract filtrate of the dysentery stopping grass and the secondary extract filtrate of the dysentery stopping grass, concentrating by using a reduced pressure rotary evaporator, and recovering the solvent.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103816270A (en) * | 2014-02-24 | 2014-05-28 | 毛玉麟 | Plant active composition for drug-free breeding and preparation method thereof |
| CN109170224A (en) * | 2018-11-28 | 2019-01-11 | 江西中成人药业有限公司 | A kind of natural compounding type Chinese medicine fodder of substitute antibiotics |
| CN109907166A (en) * | 2019-01-31 | 2019-06-21 | 中国农业科学院麻类研究所 | A kind of fodder compound reducing intestine of ruminants methane and ammonia emission, preparation method and feeding method |
| CN111213791A (en) * | 2020-03-12 | 2020-06-02 | 广西壮族自治区水牛研究所 | Licking brick for buffalo in lactation period and preparation method thereof |
| AU2020102547A4 (en) * | 2020-10-01 | 2020-11-19 | Changsha Broad-Ocean Bio-Science And Technique Co., Ltd. | A Natural Fodder Additive with Antidiarrheal, Antibacterial and Anti-inflammatory Effects and Its Preparation Method |
| CN112641686A (en) * | 2020-12-24 | 2021-04-13 | 天津国瑞蓝天科技有限公司 | Plant extract composition with antibacterial and anti-inflammatory functions and preparation method thereof |
| CN113142398A (en) * | 2021-04-27 | 2021-07-23 | 张自力 | Functional feed additive for ruminants and preparation method thereof |
-
2022
- 2022-06-27 CN CN202210732023.6A patent/CN115137020B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103816270A (en) * | 2014-02-24 | 2014-05-28 | 毛玉麟 | Plant active composition for drug-free breeding and preparation method thereof |
| CN109170224A (en) * | 2018-11-28 | 2019-01-11 | 江西中成人药业有限公司 | A kind of natural compounding type Chinese medicine fodder of substitute antibiotics |
| CN109907166A (en) * | 2019-01-31 | 2019-06-21 | 中国农业科学院麻类研究所 | A kind of fodder compound reducing intestine of ruminants methane and ammonia emission, preparation method and feeding method |
| CN111213791A (en) * | 2020-03-12 | 2020-06-02 | 广西壮族自治区水牛研究所 | Licking brick for buffalo in lactation period and preparation method thereof |
| AU2020102547A4 (en) * | 2020-10-01 | 2020-11-19 | Changsha Broad-Ocean Bio-Science And Technique Co., Ltd. | A Natural Fodder Additive with Antidiarrheal, Antibacterial and Anti-inflammatory Effects and Its Preparation Method |
| CN112641686A (en) * | 2020-12-24 | 2021-04-13 | 天津国瑞蓝天科技有限公司 | Plant extract composition with antibacterial and anti-inflammatory functions and preparation method thereof |
| CN113142398A (en) * | 2021-04-27 | 2021-07-23 | 张自力 | Functional feed additive for ruminants and preparation method thereof |
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Address after: 112000 fan he Zhen Hong Guang Cun, Tieling County, Tieling City, Liaoning Province Patentee after: Liaoning Shengtai Agriculture and Animal Husbandry Technology Co.,Ltd. Address before: 112000 fan he Zhen Hong Guang Cun, Tieling County, Tieling City, Liaoning Province Patentee before: Tieling Shengtai Animal Husbandry Feed Co.,Ltd. |