CN107549464A - A kind of feed addictive with preventing and treating rainbow trout vibriosis effect and application thereof - Google Patents
A kind of feed addictive with preventing and treating rainbow trout vibriosis effect and application thereof Download PDFInfo
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- CN107549464A CN107549464A CN201710595386.9A CN201710595386A CN107549464A CN 107549464 A CN107549464 A CN 107549464A CN 201710595386 A CN201710595386 A CN 201710595386A CN 107549464 A CN107549464 A CN 107549464A
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- vibriosis
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- rainbow trout
- feed addictive
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a kind of feed addictive with preventing and treating rainbow trout vibriosis effect and application thereof, the composition and its parts by weight of the additive are:15 20 parts of rheum officinale, 12 16 parts of radix scutellariae, 18 23 parts of myrobalan, 35 parts of galangal, 13 18 parts of garden burnet, 57 parts of radix glycyrrhizae, 15 20 parts of corydlis bungeana, 59 parts of granatum, 0.003 0.005 parts of sulfanilamide (SN), 0.002 0.004 parts of furazolidone, 0.05 0.07 parts of chloramphenicol.Have the beneficial effect that:Compatibility is reasonable between each composition of additive of the present invention, Chinese and Western medicine is combined, with significant cooperative synergism effect, the effect of anti-inflammation, strengthen immunity, is notable, there is significant antibiotic and sterilizing to act on the pathogen of vibriosis, quick, curative effect really, reach the purpose of preventing and treating and prevention rainbow trout vibriosis, be not likely to produce drug resistance, without any side effects.
Description
Technical field
The present invention relates to aquaculture feeds processing technique field, has preventing and treating rainbow trout vibriosis effect more particularly to one kind
Feed addictive and application thereof.
Background technology
Rainbow trout(Oncorhynchus mykiss)It is under the jurisdiction of Clupeiformes, salmon trout suborder on class taxology, it is carnivorous, it is suitable
A kind of wider cold water fishes of answering property, while be also to be distributed one of widest cultured fishes in the world.Because it has thorn
The characteristics such as less, meat is more, fine and tender taste, delicious flavour, nutritious, growth is fast, yield is high, production economy is profitable, into
Most to cultivate one of popularization object of future extensively at present.But rainbow trout often infects vibriosis during cultivating.Suffer from
The rainbow trout of vibriosis often shows as feeding rate decreased, and body colour blacks, and skin surface ulcer, anus is rubescent, gill filament anaemia hair
The symptom such as white, is observed, it is seen that liver, enteron aisle have inflammation symptom after dissection.
The pathogen for causing rainbow trout vibriosis is Vibrio anguillarum, is had the reason for morbidity a lot, it may be possible to which cultivation density is excessive, institute
Feeding bait is inferior, and food nutrition is unbalanced, and water pollution is more serious, or is infected by ingesting by the bait of pathogen contamination
This disease.Once infecting this disease in breeding process, the huge economic loss that can not be retrieved can be caused.Currently for rainbow trout vibriosis
Medicine is mostly to treat, and can be used for preventing and treating without effective medicine.Therefore, it is necessary to provide a kind of with preventing and treating vibriosis effect
Rainbow trout feed addictive, prevent and treat in rainbow trout breeding process and suffer from economic loss caused by vibriosis.At present, do not have also both at home and abroad
There is the relevant report for disclosing any rainbow trout feed addictive on preventing and treating vibriosis effect and its application.
Prior art such as Authorization Notice No. is the B of CN 102919248 Chinese invention patent, discloses vibrio bacteria and kills
Microbial inoculum, it is made up of the raw material of following percentage by weight:Minocycline 10~20%, vancomycin 5~15%, 5,6,7- tri-
Flavonol 15~25% and water surplus.Above-mentioned vibrio fischeri bactericide is reasonable in design, by minocycline, mould through the ages
Together with element reasonably compounds with Noroxylin,5,6,7-Trihydroxy flavone, there is very strong synergistic effect, it is good to control of vibriosis effect, prevent
Cure rate can reach more than 95%.But the component of vibrio fischeri bactericide is all antibiotic agents, dosage is larger, exists for a long time
Animal autogenous infection caused by antibiotic is added in feed, medicament residue, drug-fast bacteria pearl is produced, declines immunologic function of livestock and birds
The problems such as.
The content of the invention
It is an object of the invention to provide a kind of raw material sources are extensive, material effective component utilization rate is high, can prevent and
Rainbow trout vibriosis is treated, quick, curative effect really, is not likely to produce drug resistance, preparation method is simple, and easy to use has preventing and treating
Feed addictive of rainbow trout vibriosis effect and application thereof.
The present invention is directed to the problem of being mentioned in above-mentioned technology, and the technical scheme taken is:One kind has preventing and treating rainbow trout vibrios
The feed addictive of sick effect, composition and its parts by weight are:Rheum officinale 15-20 parts, radix scutellariae 12-16 parts, myrobalan 18-23 parts, Gao Liang
Ginger 3-5 parts, garden burnet 13-18 parts, radix glycyrrhizae 5-7 parts, corydlis bungeana 15-20 parts, granatum 5-9 parts, sulfanilamide (SN) 0.003-0.005 parts, furan
Mutter oxazolone 0.002-0.004 parts, chloramphenicol 0.05-0.07 parts.Compatibility is reasonable between above-mentioned each composition of additive, Chinese and Western medicine phases knot
Close, have the effect of significant cooperative synergism effect, anti-inflammation, strengthen immunity notable, one is provided for rainbow trout aquaculture
Kind has quick, curative effect really, no drug residue, is not likely to produce drug resistance, is secondary without any poison to effective medicine of vibriosis
The characteristics of effect.
A kind of preparation method with the feed addictive for preventing and treating rainbow trout vibriosis effect, specifically includes following steps:
Rheum officinale, radix scutellariae, myrobalan, galangal, garden burnet, radix glycyrrhizae, corydlis bungeana, granatum are measured by formula, medicine is added after ultramicro grinding
The water and medicinal material gross weight 0.04-0.06% active peptides that 3-6 times of material gross weight is measured, 60-80min is decocted, filtered, filtrate is dense
It is 1-1.2 to be reduced to relative density, produces concentrate, standby, and the amino acid sequence of active peptides is
HSHVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR, the active peptides are adsorbed in medicinal powder cell membrane surface by electrostatic interaction,
Being inserted into cell membrane causes cell membrane penetration to sexually revise, and forms transmembrane channel, and the ion channel of formation can make external environment condition
Moisture is penetrated into, and internal active ingredient is oozed out, and cell membrane disintegration, the active drug composition quick release for making medicinal powder intracellular goes out
Come;
By in concentrate using alcohol liquor ratio as 2-4:1 adds ethanol solution, extracts 5-7h, centrifugation, then supernatant liquid exists
Flowing steam 20-40min at 98-105 DEG C, ethanol is reclaimed, produces decoction, the concentration of alcohol of step recovery can reach 95%
More than, it secondary can use, avoid the waste of resource, save production cost;
Sulfanilamide (SN), furazolidone and chloramphenicol will be added in decoction, then dried, produce the feeding with preventing and treating rainbow trout vibriosis effect
Feed additives, the additive can improve immunity of organisms, eliminate free radical, have stimulation of host non-specific resistance, immune spy
The characteristic of different reaction;Can have to the pathogen of vibriosis aobvious with the infringement of microorganism outside defense cell and its toxin to body
The antibiotic and sterilizing effect of work, can reach the purpose of preventing and treating and prevention rainbow trout vibriosis.
A kind of purposes with the feed addictive for preventing and treating rainbow trout vibriosis effect:The feed addictive is directly added
Fed in feed, addition is the 1-3% of feed relative, the addition of feed addictive cause the feed have it is clearing heat and detoxicating,
The effect of antibacterial is sterilized, can be eliminated into the pathogen in fresh-water fishes body and parasite, the immune of fresh-water fishes itself can be improved
Ability and resistance against diseases, there is the effect of preventing and treating vibriosis.
Compared with prior art, the advantage of the invention is that:Compatibility is reasonable between each composition of additive of the present invention, Chinese and Western medicine
It is combined, has the effect of significant cooperative synergism effect, anti-inflammation, strengthen immunity notable, to the pathogen of vibriosis
Acted on significant antibiotic and sterilizing, reach the purpose of preventing and treating and prevention rainbow trout vibriosis;The additive has to vibriosis to be seen
The advantages of effect is fast, curative effect really, wherein antibiotic addition very pettiness, it is not likely to produce drug resistance, without any side effects;This adds
Add agent raw material sources wide, it is cheap, it is added in feed and feed palatability and nutritional ingredient is had no adverse effects, preparation method
Simply, it is adapted to the needs of scale industrial production.
Specific embodiment
The present invention program is described further below by embodiment:
Embodiment 1:
A kind of feed addictive with preventing and treating rainbow trout vibriosis effect, composition and its parts by weight are:18 parts of rheum officinale, radix scutellariae 14
Part, 20 parts of myrobalan, 4 parts of galangal, 15 parts of garden burnet, 6 parts of radix glycyrrhizae, 18 parts of corydlis bungeana, 7 parts of granatum, 0.004 part of sulfanilamide (SN), furans
0.003 part of oxazolone, 0.06 part of chloramphenicol.Compatibility is reasonable between above-mentioned each composition of additive, and Chinese and Western medicine is combined, and has significant
Cooperative synergism effect, notable the effect of anti-inflammation, strengthen immunity, provided for rainbow trout aquaculture a kind of has to vibriosis
Medicine is imitated, there is the characteristics of quick, curative effect really, no drug residue, be not likely to produce drug resistance, be without any side effects.
A kind of preparation method with the feed addictive for preventing and treating rainbow trout vibriosis effect, specifically includes following steps:
1)Rheum officinale, radix scutellariae, myrobalan, galangal, garden burnet, radix glycyrrhizae, corydlis bungeana, granatum, ultramicro grinding to medicinal powder are measured by formula
Particle diameter is 350 mesh, then adds the water of 4.5 times of amounts of medicinal material gross weight and the active peptides of medicinal material gross weight 0.05%, is heated to boiling
70min is decocted after rising, by 6 layers of filtered through gauze, then it is 1.1 to concentrate the filtrate to relative density, produces concentrate, standby, living
The amino acid sequence of property polypeptide is HSHVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR, and the active peptides pass through electrostatic interaction
Be adsorbed in medicinal powder cell membrane surface, being inserted into cell membrane causes cell membrane penetration to sexually revise, formed transmembrane channel, formation from
Subchannel can be penetrated into the moisture of external environment condition, and internal active ingredient is oozed out, cell membrane disintegration, make intracellular effective of medicinal powder
Drug ingedient quick release comes out;
2)By in concentrate using alcohol liquor ratio as 3:1 adds ethanol solution, and 6h is extracted in stirring, is placed in 8000r/min centrifugation
25min is centrifuged in machine, then by supernatant liquid at 100 DEG C flowing steam 30min, to ethanol reclaim, produce decoction, the step
The concentration of alcohol of recovery can reach more than 95%, secondary can use, and avoid the waste of resource, save production cost;
3)Sulfanilamide (SN), furazolidone and chloramphenicol will be added in decoction, then dried, produced with preventing and treating rainbow trout vibriosis effect
Feed addictive, the additive can improve immunity of organisms, eliminate free radical, have stimulation of host non-specific resistance, be immunized
The characteristic of idiosyncrasy;Can have with the infringement of microorganism outside defense cell and its toxin to body to the pathogen of vibriosis
Significant antibiotic and sterilizing effect, can reach the purpose of preventing and treating and prevention rainbow trout vibriosis.
A kind of purposes with the feed addictive for preventing and treating rainbow trout vibriosis effect:The feed addictive is directly added
Fed in feed, addition is the 2% of feed relative, the addition of feed addictive cause the feed have it is clearing heat and detoxicating, kill
The effect of bacterium antibacterial, the immunity energy that fresh-water fishes itself can be improved into the pathogen in fresh-water fishes body and parasite can be eliminated
Power and resistance against diseases, there is the effect of preventing and treating vibriosis.
Embodiment 2:
A kind of feed addictive with preventing and treating rainbow trout vibriosis effect, composition and its parts by weight are:17 parts of rheum officinale, radix scutellariae 15
Part, 19 parts of myrobalan, 5 parts of galangal, 13 parts of garden burnet, 7 parts of radix glycyrrhizae, 17 parts of corydlis bungeana, 9 parts of granatum, 0.003 part of sulfanilamide (SN), furans
0.004 part of oxazolone, 0.05 part of chloramphenicol.
It is a kind of there is preventing and treating rainbow trout vibriosis effect the preparation method of feed addictive be:
1)Rheum officinale, radix scutellariae, myrobalan, galangal, garden burnet, radix glycyrrhizae, corydlis bungeana, granatum are measured by formula, is added after ultramicro grinding
The water of 4 times of amounts of medicinal material gross weight and the active peptides of medicinal material gross weight 0.04%, decoct 65min, filtering, filtrate is concentrated into relatively
Density is 1, produces concentrate, standby, and the amino acid sequence of active peptides is
HSHVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR, the active peptides are adsorbed in medicinal powder cell membrane surface by electrostatic interaction,
Being inserted into cell membrane causes cell membrane penetration to sexually revise, and forms transmembrane channel, and the ion channel of formation can make external environment condition
Moisture is penetrated into, and internal active ingredient is oozed out, and cell membrane disintegration, the active drug composition quick release for making medicinal powder intracellular goes out
Come;
2)By in concentrate using alcohol liquor ratio as 2.5:1 adds ethanol solution, extracts 7h, centrifugation, then supernatant liquid exists
Flowing steam 35min at 98 DEG C, ethanol is reclaimed, produces decoction, the concentration of alcohol of step recovery can reach more than 95%, can
Secondary use, the waste of resource is avoided, save production cost;
3)Sulfanilamide (SN), furazolidone and chloramphenicol will be added in decoction, then dried, produced with preventing and treating rainbow trout vibriosis effect
Feed addictive, the additive can improve immunity of organisms, eliminate free radical, have stimulation of host non-specific resistance, be immunized
The characteristic of idiosyncrasy;Can have with the infringement of microorganism outside defense cell and its toxin to body to the pathogen of vibriosis
Significant antibiotic and sterilizing effect, can reach the purpose of preventing and treating and prevention rainbow trout vibriosis.
A kind of purposes with the feed addictive for preventing and treating rainbow trout vibriosis effect:The feed addictive is directly added
Fed in feed, addition is the 3% of feed relative, the addition of feed addictive cause the feed have it is clearing heat and detoxicating, kill
The effect of bacterium antibacterial, the immunity energy that fresh-water fishes itself can be improved into the pathogen in fresh-water fishes body and parasite can be eliminated
Power and resistance against diseases, there is the effect of preventing and treating vibriosis.
Embodiment 3:
A kind of feed addictive with preventing and treating rainbow trout vibriosis effect, composition and its parts by weight are:16 parts of rheum officinale, radix scutellariae 16
Part, 18 parts of myrobalan, 5 parts of galangal, 14 parts of garden burnet, 7 parts of radix glycyrrhizae, 16 parts of corydlis bungeana, 8 parts of granatum, 0.003 part of sulfanilamide (SN), furans
0.004 part of oxazolone, 0.05 part of chloramphenicol.
It is a kind of there is preventing and treating rainbow trout vibriosis effect the preparation method of feed addictive be:Rheum officinale, Huang are measured by formula
A kind of reed mentioned in ancient books, myrobalan, galangal, garden burnet, radix glycyrrhizae, corydlis bungeana, granatum, the water and medicine of 6 times of amounts of medicinal material gross weight are added after ultramicro grinding
The active peptides of material gross weight 0.06%, decoct 60min, filtering, filtrate is concentrated into relative density as 1.2, by concentrate with alcohol
Liquor ratio is 4:1 add ethanol solution, extract 5h, centrifugation, then by supernatant liquid at 100 DEG C flowing steam 25min, it is right
Ethanol reclaims, and sulfanilamide (SN), furazolidone and chloramphenicol will be added in gained decoction, then dried, and produces with preventing and treating rainbow trout vibrios
The feed addictive of sick effect.
A kind of purposes with the feed addictive for preventing and treating rainbow trout vibriosis effect:The feed addictive is directly added
Fed in feed, addition is the 1.5% of feed relative.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Tonghua Teachers College
<120>A kind of feed addictive with preventing and treating rainbow trout vibriosis effect and application thereof
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 33
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Val Ser His Arg Gly Arg Cys Tyr Cys Arg Cys Leu Arg
1 5 10 15
Cys Arg Val Leu His Pro Gly Lys Leu Cys Val Cys Val Asn Cys Ser
20 25 30
Arg
Claims (9)
- A kind of 1. feed addictive with preventing and treating rainbow trout vibriosis effect, it is characterised in that:The composition of described additive and Its parts by weight is:Rheum officinale 15-20 parts, radix scutellariae 12-16 parts, myrobalan 18-23 parts, galangal 3-5 parts, garden burnet 13-18 parts, radix glycyrrhizae 5- 7 parts, corydlis bungeana 15-20 parts, granatum 5-9 parts, sulfanilamide (SN) 0.003-0.005 parts, furazolidone 0.002-0.004 parts, chloramphenicol 0.05-0.07 parts.
- A kind of 2. feed addictive with preventing and treating rainbow trout vibriosis effect according to claim 1, it is characterised in that:Institute The preparation method processed for the additive stated is:1)Rheum officinale, radix scutellariae, myrobalan, galangal, garden burnet, radix glycyrrhizae, corydlis bungeana, granatum are measured by formula, adds water after ultramicro grinding And active peptides, decoct, filter, concentration, produce concentrate, it is standby;2)Ethanol solution extraction will be added in concentrate, centrifugation, then ethanol is reclaimed, produces decoction;3)Sulfanilamide (SN), furazolidone and chloramphenicol will be added in decoction, then dried, produced with preventing and treating rainbow trout vibriosis effect Feed addictive.
- 3. a kind of preparation method with the feed addictive for preventing and treating rainbow trout vibriosis effect according to claim 2, its It is characterised by:The addition of active peptides is medicinal powder weight 0.04-0.06% in described step 1, and its amino acid sequence is HSHVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR。
- 4. a kind of preparation method with the feed addictive for preventing and treating rainbow trout vibriosis effect according to claim 2, its It is characterised by:The addition of described step 1 reclaimed water is that 3-6 times of medicinal material gross weight is measured.
- 5. a kind of preparation method with the feed addictive for preventing and treating rainbow trout vibriosis effect according to claim 2, its It is characterised by:60-80min is decocted in described step 1.
- 6. a kind of preparation method with the feed addictive for preventing and treating rainbow trout vibriosis effect according to claim 2, its It is characterised by:It is 1-1.2 that filtrate, which is concentrated into relative density, in described step 1.
- 7. a kind of preparation method with the feed addictive for preventing and treating rainbow trout vibriosis effect according to claim 2, its It is characterised by:Alcohol liquor ratio is 2-4 in described step 2:1, extract 5-7h.
- 8. a kind of preparation method with the feed addictive for preventing and treating rainbow trout vibriosis effect according to claim 2, its It is characterised by:Ethanol recovered temperature is 98-105 DEG C in described step 2, time 20-40min.
- A kind of 9. purposes with the feed addictive for preventing and treating rainbow trout vibriosis effect, it is characterised in that:Described purposes is:Will The feed addictive directly addition is fed in feed, and addition is the 1-3% of feed relative.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110856479A (en) * | 2018-08-25 | 2020-03-03 | 谢金燕 | Super immunization method for grass carps |
-
2017
- 2017-07-20 CN CN201710595386.9A patent/CN107549464A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110856479A (en) * | 2018-08-25 | 2020-03-03 | 谢金燕 | Super immunization method for grass carps |
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