CN109329596A - The Chinese herbal feed additive and its feeding method of piglet immunological and growth can be improved - Google Patents
The Chinese herbal feed additive and its feeding method of piglet immunological and growth can be improved Download PDFInfo
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- CN109329596A CN109329596A CN201811340340.3A CN201811340340A CN109329596A CN 109329596 A CN109329596 A CN 109329596A CN 201811340340 A CN201811340340 A CN 201811340340A CN 109329596 A CN109329596 A CN 109329596A
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3481—Organic compounds containing oxygen
- A23L3/3508—Organic compounds containing oxygen containing carboxyl groups
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Chemical Kinetics & Catalysis (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
- Nutrition Science (AREA)
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Abstract
The Chinese herbal feed additive of piglet immunological and growth can be improved in the present invention, with 3-8 parts of Rhizoma Atractylodis Macrocephalae, 5-10 parts of Radix Astragali, 1-5 parts of radix saposhnikoviae, Radix Ophiopogonis 1-5 part, 15-20 parts of Medicated Leaven, 10-15 parts of 25-33 parts of malt, 10-20 parts of Radix Codonopsis, hawthorn components are compounded, and prewired No. 1 material is mixed to get with carrier, prewired No. 2 material being uniformly mixed again with remaining carrier and auxiliary material, it is followed by granulated, dried and screened, Chinese herbal feed additive is made.Feeding method specifically: 4-5g Chinese herbal feed additive, feeding cycle 10-15d are added in the piglet cradling stage, every 100 grams of feeds；In the growing and fattening pigs stage, the above-mentioned composite feed additive of 2-4g, feeding cycle 45-60d is added in every 100g feed.Chinese herbal feed additive of the present invention can play the role of improving pigling immunity, and the average daily gain of pig and efficiency of feed utilization can be improved, and can be used as the substitute of antibiotics additive.
Description
Technical field
The present invention relates to Chinese herbal feed additives and its feeding method that piglet immunological and growth can be improved, particularly belong to
Field of feed additive technology.
Background technique
China is Animal husbandry production big country, and pork yield and consumption figure are constantly in the first in the world.As animal husbandry is to collection
There is the phenomenon that Misuse antibacterials and violated veterinary drug in reduction, large-scale development, more seriously environmental pollution
Aggravation, oneself seriously threatens and challenges through the quality safety formation to pork.It is existing a large amount of research shows that Chinese herbal medicine is made both at home and abroad
It for feed addictive, can not only prevent and cure diseases, promote growth of animal, and have the characteristics that have no toxic side effect, have no drug resistance.
Chinese herbal medicine is the peculiar resource in China, has spleen benefiting and stimulating the appetite, strengthening vital QI to eliminate pathogenic factors, antibacterial growth promoting, enhancing animal immune ability, improves livestock products
The advantages of the advantages that product quality, as long as selection is proper, compatibility is reasonable, nontoxic, without drug resistance is that antibiotic is incomparable.
With being widely used for antibiotic, the residue problem in immunity of livestock decline and livestock products and environment is highlighted.It is existing
There is feed addictive often single variety, effect is also more single, is not able to satisfy the needs for improving immunity and growth performance.Cause
This, finding has the compound additive substitute for improving immunity of organisms and growth performance, it has also become pig-breeding industry health
The important trends of sustainable development.It is reported that Chinese herbal medicine immunity substance mass-energy enhances human body immune function, animal is improved
Resisting stress, anti-disease ability, to reduce animal husbandry to the pollution of environment, develop unpolluted animal husbandry and meet people and food is pacified
Full demand plays a significant role.The day of piglet, growing and fattening pigs can be improved compared with antibiotic for the research Chinese herb feed additive such as Dai Rongguo
Weight gain, the average daily gain of each Chinese medicine group are all higher than control group.Tian Yunbo etc. utilizes several kinds of Chinese medicinal materials basic components, grows pig
Daily gain and feed conversion rate etc. are all higher than antibiotic group.Li Siyuan etc. is raw with feedings such as fruit dried orange peel, the fleece-flower root, pine needle meals
Long pig can promote appetite, increase feed intake, promote the growth and development of pig.Xue Fu even etc. report, garlic, capsicum, nutmeg,
The pungent feeding dorking of pepper, cloves, ginger can improve the quality of chicken, so that the chicken fragrance is thickened.Chen Jinshun etc. is in " Foshan science
Technical college's journal " 2002 years the 3rd interim reports, adding the additive containing herbal mixture effective component can extremely significant raising flesh
Between fat content, improve the fragrance of meat, increase the tenderness of meat.Child's foundation etc. is in Hua Zhong Agriculture University's [master] paper in 2005
Report Chinese herbal medicine extract group can improve the speed of growth and the price of deed of growing-finishing pig, improve disease resistance, the trunk of pig
Body lean meat percentage improves meat quality of fattening pigs.Hayen etc., Peng Huizhen etc. and Zhu Nanshan etc. are reported respectively, and it is more that yeast is added in daily ration
Sugar and soluble polysaccharide, can significantly promote daily ration weight gain and the feed conversion rate of pig, chicken, ox etc., and can reduce the death of animal
Rate.It is reported above demonstrate Chinese herb feed additive promote pig growth and improve in terms of have advantage and its
The theoretical basis of application in the production of green ecological pork.But in application process of the Chinese herbal medicine as feed addictive,
The problems such as not scientific enough there is also higher cost, unstable quality, formula standard, main cause are medicinal herb components complexity,
Various regions medicinal plant component content is not identical；The inspection testing method traditional to Chinese herbal medicine China is that naked eyes are seen, mouth is tasted, hand is touched
Deng quality discrepancy is larger.The author in view of the above-mentioned problems, carried out the screening test of the prescription of immunological enhancement, as a result first
It is certain to show that the Chinese herbal medicine compound (3-8 parts of Rhizoma Atractylodis Macrocephalae, 5-10 parts of Radix Astragali, 1-5 parts of radix saposhnikoviae, Radix Ophiopogonis 1-5 part) in this patent has
Immunological enhancement, especially to the immune response after piglet vaccine immunity have good effect.It finds under study for action at this time
The growth performance of the pig of the Chinese herbal medicine compound has been used to receive certain influence, it is slow that growth and development occurs in only a few individual
Situation increases some other medicinal herb components, finally for this purpose, the author compounds the Chinese herbal medicine compound again
It realizes and immunity and somatotrophic additivity benefit is provided.Present invention application combined Chinese herb passes through at composite feed additive
In the mode that piglet and growing and fattening pigs two stages feed, immune response caused by piglet stage injection vaccine can be reduced, it can be significant
The immunity and growth performance for improving growing and fattening pigs, have better conformed to the physilogical characteristics and growth-development law of animal, to realize
Chinese herbal feed additive part substitute antibiotics have positive effect to promoting modern farming to develop in a healthy way.
Summary of the invention
The present invention proposes a kind of Chinese herbal feed additive that piglet immunological and growth can be improved and its feeding method method, mesh
Be: respectively in piglet stage, fattening stage, through the Chinese medicine composition of addition compounding in feed, play and improve piglet
Immunity, while average daily gain and the efficiency of feed utilization of pig are improved, it can be as the substitute of antibiotics additive.
It is clearing heat and detoxicating, QI invigorating, anti-inflammatory in Chinese herbal medicine formula of the present invention based on Rhizoma Atractylodis Macrocephalae, Radix Astragali, radix saposhnikoviae, Radix Ophiopogonis, promote to exempt from
Epidemic disease function, and play the role of certain removing free radical, anti-oxidant, anti-heat stress；It is with Medicated Leaven, malt and codonopsis pilosula, hawthorn
It is auxiliary, the palatability of feed is adjusted, environment in gastrointestinal tract is improved, promotes digestion and absorption of the animal to nutriment, prevention growth
It is obstructed, feed intake decline, the diseases such as diarrhea.
The Chinese herbal feed additive of piglet immunological and growth can be improved in the present invention, by the Chinese herbal medicine of addition compounding to mention
The immune and growth of high piglet；Its preparation step is specific as follows:
Step 1: according to the mass fraction, Chinese herbal medicine and carrier number control it is as follows: Rhizoma Atractylodis Macrocephalae 3-8, Radix Astragali 5-10, radix saposhnikoviae 1-5,
Radix Ophiopogonis 1-5, Medicated Leaven 15-20, malt 25-33, Radix Codonopsis 10-20, hawthorn 10-15, carrier 900-950；Said herbal medicine is pressed
Ratio mixing crushes, crosses 80 meshes, is uniformly mixed with carrier of mass fractions is waited thereafter, obtains prewired No. 1 material；
Step 2: the mass ratio of prewired No. 1 material of control and auxiliary material is 1: 1~2: 1, by above-mentioned prewired No. 1 material, auxiliary
The carrier of remaining number is uniformly mixed in raw material, step 1, obtains prewired No. 2 material；
Step 3: prewired No. 2 material being granulated, dried and crossed 40 meshes, composite feed additive is made；
The auxiliary material is according to the mass fraction: multi-vitamins 0.03, antioxidant 0.03, high temperature resistant phytase
0.02, complex enzyme 0.02, lysine 0.4, methionine 0.1, threonine 0.1, tryptophan 0.03, pig composite trace element 0.1,
50wt% choline chloride 0.1, zinc oxide 0.05, calcium monohydrogen phosphate 1.0, mountain flour 0.6, sodium chloride 0.3, saccharin sodium 0.02, mould inhibitor
0.1；
The carrier is the mixture of corn protein powder and chaff of uniting, and corn protein powder and system chaff mass ratio are 1: 1.
The feeding method are as follows: the addition of 4-5g Chinese herbal feed is added in the piglet cradling stage, every 100 grams of feeds
Agent, feeding cycle 10-15d；In the growing and fattening pigs stage, the above-mentioned composite feed additive of 2-4g, feeding week is added in every 100g feed
Phase is 45-60d.
The complex enzyme is the mixture of zytase, protease, 'beta '-mannase and amylase.
The mould inhibitor ingredient is the mixture of propionic acid, formic acid.
Beneficial effects of the present invention:
Chinese herbal feed additive of the present invention can propose immune response of the significantly high piglet to vaccine in pig starter feed；?
Fattening stage uses, and the health pig speed of growth can be made fast, and feedstuff-meat ratio is low, increases day by day great, and pig body can be improved and resist the external world
The adaptability of environmental change is conducive to the healthy growth of pig.In addition, being produced using the pork that this Chinese herbal feed additive produces
Product are remained without any antibiotic.
Compared with prior art, present invention has the advantage that
1, Chinese herb feed additive of the invention is added, then can effectively improve the palatability of feed addictive, promote adopting for pig
Appetite improves the daily gain of pig；
2, since Rhizoma Atractylodis Macrocephalae has the function of enhancing body's immunity, this feed addictive is with Rhizoma Atractylodis Macrocephalae, Radix Astragali, anti-
Wind, Radix Ophiopogonis cooperatively form formula according to a certain percentage, play a role in terms of improving humoral immunity of organism, to make pig anti-
Immune function in terms of bacterium infection, poisoning intrusion significantly increases, and can effectively improve the adaptation that pig body resists ambient pressure environment
Power is conducive to the healthy growth of pig；
3, any antibiotic is free of, bristles color and meat can be improved, reduced environmental pollution, there is interests people's living standard
It improves, meets the environmental requirement of breeding production；
4, preparation process is simple, and raw material sources are extensive, at low cost, economical and practical, is conducive to large-scale production, has good
Application prospect.
Specific embodiment
Embodiment 1
Chinese herbal medicine and carrier are mixed by quality proportioning: 6 parts of Rhizoma Atractylodis Macrocephalae, 10 parts of Radix Astragali, windproof 3 parts, 3 parts of Radix Ophiopogonis, Medicated Leaven 18
Part, 28 parts of malt, 16 parts of Radix Codonopsis, 11 parts of hawthorn, 905 parts of carrier.
Chinese medicine material in formula is crushed, 80 meshes are crossed, is uniformly mixed with carrier of mass fractions is waited, obtains prewired No. 1
Material；Auxiliary material is uniformly mixed to obtain prewired No. 2 material with the carrier of prewired No. 1 material, remaining number, then expects prewired No. 2
After being granulated, being dried, 40 meshes are crossed, Chinese herbal feed additive is made.Wherein, the quality of prewired No. 1 material and auxiliary material
Than being 1: 1.
Auxiliary material structure at according to the mass fraction: multi-vitamins 0.03, antioxidant 0.03, high temperature resistant phytase
0.02, complex enzyme 0.02, lysine 0.4, methionine 0.1, threonine 0.1, tryptophan 0.03, pig composite trace element 0.1,
50wt% choline chloride 0.1, zinc oxide 0.05, calcium monohydrogen phosphate 1.0, mountain flour 0.6, sodium chloride 0.3, saccharin sodium 0.02, mould inhibitor
0.1；
Corn protein powder in carrier is mixed with system chaff by weight 1: 1, and crushed after being dried, moisture control is small after drying
Granularity is less than 0.5mm after 2%, crushing.4g the present embodiment is added to phase feeding, every 100g feed is delivered for sale in growing and fattening pigs, that is, 60kg
Chinese herbal feed additive.
Embodiment 2
Chinese herbal medicine and carrier are mixed by quality proportioning: 4 parts of Rhizoma Atractylodis Macrocephalae, 9 parts of Radix Astragali, windproof 3 parts, 4 parts of Radix Ophiopogonis, Medicated Leaven 16
Part, 26 parts of malt, 13 parts of Radix Codonopsis, 12 parts of hawthorn, 912 parts of carrier.
Preparation method is that the Chinese medicine material in being formulated crushes, and crosses 80 meshes, is uniformly mixed with carrier of mass fractions is waited,
Obtain prewired No. 1 material；Auxiliary material is uniformly mixed with the carrier of prewired No. 1 material, remaining number obtain prewired No. 2 and be expected, then No. 2
Material is pelletized, and after dry, is crossed 40 meshes, is obtained the Chinese herbal feed additive.
Remaining is the same as embodiment 1.
Embodiment 3
Chinese herbal medicine and carrier are mixed by quality proportioning: 5 parts of Rhizoma Atractylodis Macrocephalae, 10 parts of Radix Astragali, windproof 3 parts, 3 parts of Radix Ophiopogonis, Medicated Leaven 17
Part, 25 parts of malt, 14 parts of Radix Codonopsis, 11 parts of hawthorn, 911 parts of carrier.
Preparation method is that the Chinese medicine material in being formulated crushes, and crosses 80 meshes, is uniformly mixed with carrier of mass fractions is waited,
Obtain prewired No. 1 material；Auxiliary material is uniformly mixed with the carrier of prewired No. 1 material, remaining number obtain prewired No. 2 and be expected, then No. 2
Material is pelletized, and after dry, is crossed 40 meshes, is obtained the Chinese herbal feed additive.
Remaining is the same as embodiment 1.
Embodiment 4
Chinese herbal medicine and carrier are mixed by quality proportioning: 7 parts of Rhizoma Atractylodis Macrocephalae, 10 parts of Radix Astragali, windproof 3 parts, 3 parts of Radix Ophiopogonis, Medicated Leaven 15
Part, 24 parts of malt, 16 parts of Radix Codonopsis, 10 parts of hawthorn, 914 parts of carrier.
Preparation method is that the Chinese medicine material in being formulated crushes, and crosses 80 meshes, is uniformly mixed with carrier of mass fractions is waited,
Obtain prewired No. 1 material；Auxiliary material is uniformly mixed with the carrier of prewired No. 1 material, remaining number obtain prewired No. 2 and be expected, then No. 2
Material is pelletized, and after dry, is crossed 40 meshes, is obtained the Chinese herbal feed additive.
Remaining is the same as embodiment 1.
Embodiment 5
Chinese herbal medicine and carrier are mixed by quality proportioning: 5 parts of Rhizoma Atractylodis Macrocephalae, 8 parts of Radix Astragali, windproof 2 parts, 2 parts of Radix Ophiopogonis, Medicated Leaven 18
Part, 28 parts of malt, 16 parts of Radix Codonopsis, 12 parts of hawthorn, 911 parts of carrier.
Preparation method is that the Chinese medicine material in being formulated crushes, and crosses 80 meshes, is uniformly mixed with carrier of mass fractions is waited,
Obtain prewired No. 1 material；Auxiliary material is uniformly mixed with the carrier of prewired No. 1 material, remaining number obtain prewired No. 2 and be expected, then No. 2
Material is pelletized, and after dry, is crossed 40 meshes, is obtained the Chinese herbal feed additive.
Remaining is the same as embodiment 1.
Embodiment 6
Feed addictive prepared by embodiment 1, embodiment 2, embodiment 3, embodiment 4, embodiment 5 is applied to growing and fattening pigs
Feeding trial.
6.1 experimental animals and experimental design
Test selection age in days is close, weight is close, health status consistent " Ternary Pig " ternary pigs 180, is randomly divided into 6
Group.Every group sets 3 repetitions, 10 pigs of each repetition, and the diet that repeating groups are fed is identical.It is advanced before the formal beginning of growing and fattening pigs test
Row prerun in 7 days raising, feeds basal diet (composition of basal diet is shown in Table 1) respectively, and formal test period is 45 days.
1 growing and fattening pigs basal diet of table composition
| Diet composition | Ratio |
| Corn | 65.5 |
| Dregs of beans | 14.0 |
| Soybean oil | 1.5 |
| Wheat bran | 10.0 |
| DDGS | 5.0 |
| It is total | 100 |
2 Chinese herbal feed additive of table composition
6.2 test daily rations
A group: basal diet.
B group: 1 Chinese herbal feed additive of 4g embodiment is added in every 100g feed.
C group: 2 Chinese herbal feed additive of 4g embodiment is added in every 100g feed.
D group: 3 Chinese herbal feed additive of 4g embodiment is added in every 100g feed.
E group: 4 Chinese herbal feed additive of 4g embodiment is added in every 100g feed.
F group: 5 Chinese herbal feed additive of 4g embodiment is added in every 100g feed.
6.3 feeding managements and index determining
Test pig is freely eaten and drinking-water, routinely carries out immune and expelling parasite, observes and records pig in experimental period daily
Health status.Off-test weighing, calculates each processing group daily gain, feed intake, feedstuff-meat ratio (feed intake/weight gain).
6.4 data statistic analysis
Test data carries out the one-factor analysis of variance using SSPS16.0 statistical software, is examined using Duncan method significant
Property.
6.5 test results are shown in Table 2.
Table 3
| Project | A group | B group | C group | D group | E group | F group |
| Starting weight, kg | 61.72±0.70a | 61.38±0.97a | 61.25±0.36a | 61.65±0.89a | 61.66±0.98a | 61.46±0.75a |
| End weight, kg | 92.48±0.88d | 97.37±1.14b | 97.53±0.66b | 96.46±0.64c | 98.39±0.91a | 96.90±0.56bc |
| Daily gain, g/d head | 683.47±21.75d | 799.8±14.53ab | 806.2±13.83ab | 773.38±20.03c | 816.2±10.32a | 787.69±15.97bc |
| Daily ingestion amount, g/d head | 2.56 | 2.42 | 2.46 | 2.45 | 2.41 | 2.44 |
| Feedstuff-meat ratio | 3.75:1 | 3.03:1 | 3.05:1 | 3.17:1 | 2.95:1 | 3.10:1 |
| Diarrhea rate | 26.7% | 6.7% | 3.3% | 6.7% | 3.3% | 10% |
Note: compared with the control group, same letter is not significant difference, p >=0.05；Different letters are significant difference, p≤
0.05。
Feed intake (ADFI): the feeding capacity and clout amount of 0 pig are tested in each column of accurate recording, calculate every pig being averaged weekly
Daily ingestion amount and full period average daily gain；
Daily gain (ADG): test pig starts and terminates weekly 7:30 in morning and weighs on an empty stomach, calculates every pig being averaged weekly
Daily gain and full period average daily gain；
Feedstuff-meat ratio (F/G): it is calculated according to feed intake and weight gain.
As can be seen from Table 3, Chinese herbal feed additive of the present invention can significantly improve growing and fattening pigs gaining effect, control
Also there is remarkable result in terms of diarrhea.The result shows that after feeding Chinese herbal feed additive of the present invention, can health care pig intestinal health,
That improves pig enhances weight, reduces feedstuff-meat ratio.In addition, pig health status is good during test, has loose bowels and wait enterogastric diseases phenomenon
It is less.
Embodiment 7
7.1 experimental animals and experimental design
Test selection age in days is close to (30 age in days), weight is close, health status consistent " Ternary Pig " ternary pigs 90, with
Machine is divided into 3 groups.Every group sets 3 repetitions, 10 pigs of each repetition, and the diet that repeating groups are fed is identical.Test pre-feeding period 3d, feeding
Basal diet (is shown in Table 3), the blood sampling of pre-feeding period end.Daily ration 10d is fed, 12h is weighed, is immunized after feeding, each pig
The O-shaped aftosa commercial seedling of immune 1ml, 4w carry out two and exempt from, 7w weighing, vena cava anterior blood sampling.Blood sample is stored at room temperature in 4h
Afterwards, in 4 DEG C of refrigerator overnights, 3000rpm is centrifuged 3min, draws serum and is sub-packed in 0.2ml plastic centrifuge tube, -20 DEG C of preservations, inspection
Survey antibody level.
3 child care pig basal diet of table composition
| Diet composition | Ratio |
| Corn | 60.0 |
| 43 dregs of beans | 16.0 |
| Wheat-middlings | 4.5 |
| Soybean oil | 2.0 |
| Imported fish meal | 2.5 |
| Expanded soybean | 10.0 |
| It is total | 100 |
7.2 test daily rations
A group: blank control (basal diet).
B group: 2 Chinese herbal feed additive of 5g embodiment is added in every 100g feed.
C group: 4 Chinese herbal feed additive of 5g embodiment is added in every 100g feed.
7.3, feeding management and index determining
Test pig is freely eaten and drinking-water, is routinely immunized, observes and records the health of pig in experimental period daily
Situation.Off-test weighing, measures antibody level of serum.
7.4 data statistic analysis
Test data carries out the one-factor analysis of variance using SSPS16.0 statistical software.
7.5 test results see the table below shown in 4
Influence of 4 Chinese herbal feed additive of table to aftosa vaccine immunity
Note: compared with the control group, same letter is not significant difference, p >=0.05；Different letters are significant difference, p≤
0.05。
Table 4 shows the immune preceding aftosa testing result for exempting from rear 3 weeks pigs with two of Schweineseuche O-shaped inactivated vaccine.By table 1
Known to it is immune before test each group foot-and-mouth disease antibody it is horizontal, A group, B group, the OD630nm value of C group < 0.2, lower than kit 0.4
Positive criteria；Compared with the OD630nm value of A group, B group, C group difference is not significant (P > 0.05).Two exempt to try for latter 3 weeks in table 4
The result for testing the foot-and-mouth disease antibody level of each group can be seen that B group, the OD630nm value of C group is all remarkably higher than A group (P < 0.05).
It can be seen that feeding has significant immunological enhancement to aftosa vaccine through this Chinese herbal feed additive.In addition, entire test
The body weight gains situation for testing pig in the process show to test the body weight gains of each group pig compared with the control group difference it is not significant (P >
0.05), the body weight gains of pig also no significant difference (P > 0.05) between additive group.
Claims (4)
1. the Chinese herbal feed additive of piglet immunological and growth can be improved, it is characterised in that: the Chinese herbal feed addition
Immune and growth, the preparation step that agent improves piglet by compounding Chinese herbal medicine are specific as follows:
Step 1: according to the mass fraction, Chinese herbal medicine and the control of carrier number are as follows: Rhizoma Atractylodis Macrocephalae 3-8, Radix Astragali 5-10, radix saposhnikoviae 1-5, Radix Ophiopogonis
1-5, Medicated Leaven 15-20, malt 25-33, Radix Codonopsis 10-20, hawthorn 10-15, carrier 900-950；In proportion by said herbal medicine
Mixing crushes, crosses 80 meshes, is uniformly mixed with carrier of mass fractions is waited thereafter, obtains prewired No. 1 material；
Step 2: prewired No. 1 material of control and the mass ratio of auxiliary material are 1: 1 ~ 2: 1, by above-mentioned prewired No. 1 material, auxiliary material,
The carrier of remaining number is uniformly mixed in step 1, obtains prewired No. 2 material；
Step 3: prewired No. 2 material being granulated, dried and crossed 40 meshes, composite feed additive is made；
The auxiliary material is according to the mass fraction: multi-vitamins 0.03, antioxidant 0.03, high temperature resistant phytase 0.02,
Complex enzyme 0.02, lysine 0.4, methionine 0.1, threonine 0.1, tryptophan 0.03, pig composite trace element 0.1,50wt%
Choline chloride 0.1, zinc oxide 0.05, calcium monohydrogen phosphate 1.0, mountain flour 0.6, sodium chloride 0.3, saccharin sodium 0.02, mould inhibitor 0.1；
The carrier is the mixture of corn protein powder and chaff of uniting, and corn protein powder and system chaff mass ratio are 1: 1.
2. the Chinese herbal feed additive feeding method of piglet immunological and growth can be improved, it is characterised in that: the nursing side
Method are as follows: 4-5g Chinese herbal feed additive, feeding cycle 10-15d are added in the piglet cradling stage, every 100 grams of feeds；?
The above-mentioned composite feed additive of 2-4g, feeding cycle 45-60d is added in growing and fattening pigs stage, every 100g feed.
3. the Chinese herbal feed additive according to claim 1 that piglet immunological and growth can be improved, it is characterised in that: institute
The complex enzyme stated is the mixture of zytase, protease, 'beta '-mannase and amylase.
4. the Chinese herbal feed additive according to claim 1 that piglet immunological and growth can be improved, it is characterised in that: institute
The mould inhibitor ingredient stated is the mixture of propionic acid, formic acid.
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| CN111296639A (en) * | 2020-03-30 | 2020-06-19 | 河南科技学院 | High-temperature stress resistant Chinese herbal medicine feed additive for pigs, preparation method thereof and high-temperature stress resistant pig feed |
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