Disclosure of Invention
The invention aims to provide the plant composition for preventing the dairy cow mastitis with stable and reliable effect, which has the effects of improving the immunity of the dairy cow, reducing the incidence rate of the dairy cow mastitis, improving the milk yield and the like, effectively changes the current situation that a large amount of antibiotics are used in the current dairy cow mastitis prevention and treatment link, solves the food safety hidden danger caused by drug residues, improves the quality of dairy products and improves the competitiveness of domestic dairy products.
The invention also provides a feed containing the plant composition for preventing the cow mastitis.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
the invention provides a plant composition for preventing cow mastitis, which is characterized by comprising the following components in parts by weight: 1-10 parts of dandelion crushed material, 1-10 parts of liquorice crushed material, 1-10 parts of burdock crushed material, 1-10 parts of red clover crushed material, 1-10 parts of cowherb seed crushed material, 1-10 parts of snakegourd fruit crushed material, 1-10 parts of eucommia bark crushed material and 1-10 parts of sweetgum fruit crushed material.
The plant composition for preventing the cow mastitis is characterized by comprising the following components in parts by weight: 1-10 parts of dandelion crushed material, 1-10 parts of licorice crushed material, 1-10 parts of burdock crushed material, 1-10 parts of red clover crushed material, 1-10 parts of cowherb seed crushed material, 1-10 parts of snakegourd fruit crushed material, 1-10 parts of eucommia bark crushed material, 1-10 parts of sweetgum fruit crushed material, 1-10 parts of frankincense crushed material, 1-10 parts of myrrh crushed material, 1-10 parts of akebia stem crushed material, 1-10 parts of honeysuckle flower crushed material, 1-10 parts of platycodon root crushed material, 1-10 parts of epimedium herb crushed material and 1-10 parts of alfalfa crushed material.
The plant composition for preventing the cow mastitis is characterized in that the composition is added with acceptable auxiliary materials to prepare a clinically acceptable preparation, and the preparation is powder or granules.
The invention also provides a feed containing the plant composition for preventing the cow mastitis, which is characterized by comprising the following components in parts by weight: 0.1-1.0% of the plant composition for preventing the cow mastitis and 99.0-99.9% of auxiliary materials.
The invention provides another plant composition for preventing dairy cow mastitis, which is characterized by comprising the following components in parts by weight: 1-10 parts of dandelion alcohol extract, 1-10 parts of liquorice water extract, 1-10 parts of burdock alcohol extract, 1-10 parts of red clover alcohol extract, 1-10 parts of cowherb seed alcohol extract, 1-10 parts of snakegourd fruit alcohol extract, 1-10 parts of eucommia alcohol extract and 1-10 parts of fructus liquidambaris water extract.
The other plant composition for preventing the cow mastitis is characterized by comprising the following components in parts by weight: 1-10 parts of dandelion alcohol extract, 1-10 parts of liquorice water extract, 1-10 parts of burdock alcohol extract, 1-10 parts of red clover alcohol extract, 1-10 parts of cowherb seed alcohol extract, 1-10 parts of snakegourd fruit alcohol extract, 1-10 parts of eucommia bark alcohol extract, 1-10 parts of fructus liquidambaris water extract and 1-10 parts of frankincense alcohol extract, 1-10 parts of myrrh alcohol extract, 1-10 parts of akebia stem alcohol extract, 1-10 parts of honeysuckle alcohol extract, 1-10 parts of platycodon grandiflorum water extract, 1-10 parts of epimedium alcohol extract and 1-10 parts of alfalfa alcohol extract.
The other plant composition for preventing the cow mastitis is characterized in that the composition is added with acceptable auxiliary materials to prepare a clinically acceptable preparation, and the preparation is powder, water or granules.
The invention also provides another feed containing the other plant composition for preventing the dairy cow mastitis, which is characterized by comprising the following components in parts by weight: 0.02-0.20% of another plant composition for preventing cow mastitis and 99.80-99.98% of auxiliary materials.
The invention provides a plant composition for preventing cow mastitis, which has the following advantages:
1. the plant composition for preventing the dairy cow mastitis is characterized in that the plant composition is selected from plants with the functional components of clearing heat, removing toxicity, sterilizing and improving the immunity of the organism according to the causes of the recessive mastitis and the dominant mastitis of the dairy cow, the compatibility of selected materials is scientific and reasonable, and after the plant composition is absorbed by the organism, the immunity of the dairy cow can be improved, the morbidity of the mastitis is reduced, the milk yield is improved, and the milk quality is improved.
2. The plant composition has rich sources, simple preparation method and low cost, and is suitable for the requirement of large-scale industrial production; the veterinary drug is convenient to use, free of drug residue and toxic and side effects, meets the requirements of people on safe production of livestock products, and has a wide market prospect.
3. The plant composition for preventing the cow mastitis can also be used as a veterinary drug by increasing the using dosage.
Detailed Description
Examples 1 to 5 preparation of a powder of a plant composition for preventing mastitis in dairy cows
Table 1 shows a specific formulation of the plant composition for preventing mastitis in dairy cows of example 1 to example 5.
TABLE 15 specific formulations of examples 1 to 5 of the present invention
Preparation of a1 in example 1:
(1) respectively drying the cleaned dandelion, liquorice, burdock, red clover, cowherb seed, snakegourd fruit, eucommia bark and beautiful sweetgum fruit for 5 hours at the temperature of 40-50 ℃;
(2) respectively pulverizing dried herba Taraxaci, Glycyrrhrizae radix, fructus Arctii, herba Trifolii Pratentis, semen Vaccariae, fructus Trichosanthis, Eucommiae cortex and fructus Lipuidambaris, and sieving with 200 mesh sieve to obtain herba Taraxaci pulverized material, Glycyrrhrizae radix pulverized material, fructus Arctii pulverized material, herba Trifolii Pratentis pulverized material, semen Vaccariae pulverized material, fructus Trichosanthis pulverized material, Eucommiae cortex pulverized material and fructus Lipuidambaris pulverized material;
(3) mixing and stirring 10 parts by weight of dandelion crushed material, 5 parts by weight of liquorice crushed material, 3 parts by weight of burdock fruit crushed material, 5 parts by weight of red clover crushed material, 2 parts by weight of cowherb seed crushed material, 2 parts by weight of snakegourd fruit crushed material, 10 parts by weight of eucommia bark crushed material and 3 parts by weight of sweetgum fruit crushed material for 15min to ensure that the variation coefficient of the uniformity is less than 5 percent.
Preparation method of a2 in example 2:
(1) respectively drying the cleaned dandelion, liquorice, burdock, red clover, cowherb seed, snakegourd fruit, eucommia bark, beautiful sweetgum fruit, frankincense and akebia stem for 5 hours at the temperature of 40-50 ℃;
(2) respectively crushing the dried dandelion, liquorice, burdock, red clover, cowherb seed, snakegourd fruit, eucommia bark, beautiful sweetgum fruit, frankincense and akebia stem, and then sieving the crushed dandelion, the crushed reed rhizome, the crushed lophatherum gracile, the crushed fibraurea stem, the crushed liquorice, the crushed burdock fruit, the crushed red clover, the crushed cowherb seed, the crushed snakegourd fruit, the crushed eucommia bark, the crushed sweetgum fruit, the crushed frankincense and the crushed akebia stem by a 250-mesh sieve to obtain the dandelion, the crushed reed rhizome, the crushed lophatherum gracile, the crushed fibraurea stem, the crushed liquorice, the crushed burdock fruit, the crushed red clover, the crushed cowherb seed, the crushed snakegourd fruit, the;
(3) mixing and stirring 3 parts by weight of dandelion crushed material, 6 parts by weight of liquorice crushed material, 2 parts by weight of burdock crushed material, 4 parts by weight of red clover crushed material, 3 parts by weight of cowherb seed crushed material, 1 part by weight of snakegourd fruit crushed material, 8 parts by weight of eucommia bark crushed material, 2 parts by weight of sweetgum fruit crushed material and 3 parts by weight of frankincense crushed material for 14min, and enabling the variation coefficient of the uniformity to be less than 5%.
Preparation of a3 in example 3:
(1) respectively drying the cleaned dandelion, liquorice, burdock, red clover, cowherb seed, snakegourd fruit, eucommia bark, beautiful sweetgum fruit, frankincense, myrrh and honeysuckle for 5 hours at the temperature of 40-50 ℃;
(2) respectively pulverizing dried herba Taraxaci, Glycyrrhrizae radix, fructus Arctii, herba Trifolii Pratentis, semen Vaccariae, fructus Trichosanthis, Eucommiae cortex, fructus Lipuidambaris, Olibanum, Myrrha and flos Lonicerae, and sieving with 280 mesh sieve to obtain herba Taraxaci pulverized product, Glycyrrhrizae radix pulverized product, fructus Arctii pulverized product, herba Trifolii pulverized product, semen Vaccariae pulverized product, fructus Trichosanthis pulverized product, Eucommiae cortex pulverized product, fructus Lipuidambaris pulverized product, Olibanum pulverized product, Myrrha pulverized product and flos Lonicerae pulverized product;
(3) mixing and stirring 6 parts by weight of dandelion crushed material, 3 parts by weight of liquorice crushed material, 2 parts by weight of burdock crushed material, 5 parts by weight of red clover crushed material, 4 parts by weight of cowherb seed crushed material, 2 parts by weight of snakegourd fruit crushed material, 3 parts by weight of eucommia bark crushed material, 5 parts by weight of sweetgum fruit crushed material, 6 parts by weight of frankincense crushed material, 2 parts by weight of myrrh crushed material and 3 parts by weight of honeysuckle crushed material for 16min to ensure that the variation coefficient of the uniformity is less than 5 percent.
Preparation of a4 in example 4:
(1) respectively drying the cleaned dandelion, liquorice, burdock, red clover, cowherb seed, snakegourd fruit, eucommia bark, beautiful sweetgum fruit, frankincense, myrrh, platycodon root and alfalfa at 40-50 ℃ for 4 hours;
(2) respectively pulverizing dried herba Taraxaci, Glycyrrhrizae radix, fructus Arctii, herba Trifolii Pratentis, semen Vaccariae, fructus Trichosanthis, Eucommiae cortex, fructus Lipuidambaris, Olibanum, Myrrha, radix Platycodi and herba Medicaginis, and sieving with 220 mesh sieve to obtain herba Taraxaci pulverized product, Glycyrrhrizae radix pulverized product, fructus Arctii pulverized product, herba Trifolii Pratentis pulverized product, semen Vaccariae pulverized product, fructus Trichosanthis pulverized product, Eucommiae cortex pulverized product, fructus Lipuidambaris pulverized product, Olibanum pulverized product, Myrrha pulverized product, radix Platycodi pulverized product and herba Medicaginis pulverized product;
(3) mixing and stirring 3 parts by weight of dandelion crushed material, 5 parts by weight of liquorice crushed material, 3 parts by weight of burdock crushed material, 3 parts by weight of red clover crushed material, 3 parts by weight of cowherb seed crushed material, 1 part by weight of snakegourd fruit crushed material, 5 parts by weight of eucommia bark crushed material, 3 parts by weight of sweetgum fruit crushed material, 3 parts by weight of frankincense crushed material, 2 parts by weight of myrrh crushed material, 6 parts by weight of platycodon root crushed material and 8 parts by weight of alfalfa crushed material for 18min, and enabling the variation coefficient of the uniformity to be less than 5 percent.
Preparation of a5 in example 5:
(1) respectively drying the cleaned dandelion, liquorice, burdock, red clover, cowherb seed, snakegourd fruit, eucommia bark, beautiful sweetgum fruit, frankincense, myrrh, akebia stem, honeysuckle, platycodon root, epimedium and alfalfa at 40-50 ℃ for 5 hours;
(2) oven-drying herba Taraxaci, Glycyrrhrizae radix, fructus Arctii, herba Trifolii Pratentis, semen Vaccariae, fructus Trichosanthis, Eucommiae cortex, fructus Lipuidambaris, Olibanum, Myrrha, and fructus Lycii,
Respectively pulverizing caulis Akebiae, flos Lonicerae, radix Platycodi, herba Epimedii and herba Medicaginis, and sieving with 300 mesh sieve to obtain herba Taraxaci pulverized material, Glycyrrhrizae radix pulverized material, fructus Arctii pulverized material, herba Trifolii Pratentis pulverized material, semen Vaccariae pulverized material, fructus Trichosanthis pulverized material, Eucommiae cortex pulverized material, fructus Lipuidambaris pulverized material, Olibanum pulverized material, Myrrha pulverized material, caulis Akebiae pulverized material, flos Lonicerae pulverized material, radix Platycodi pulverized material, herba Epimedii pulverized material and herba Medicaginis pulverized material;
(3) mixing and stirring 6 parts by weight of dandelion crushed material, 4 parts by weight of liquorice crushed material, 5 parts by weight of burdock crushed material, 3 parts by weight of red clover crushed material, 3 parts by weight of cowherb seed crushed material, 8 parts by weight of snakegourd fruit crushed material, 4 parts by weight of eucommia bark crushed material, 1 part by weight of sweetgum fruit crushed material, 4 parts by weight of frankincense crushed material, 5 parts by weight of myrrh crushed material, 1 part by weight of akebia stem crushed material, 4 parts by weight of honeysuckle crushed material, 2 parts by weight of platycodon root crushed material, 3 parts by weight of epimedium crushed material and 2 parts by weight of alfalfa crushed material for 20min to ensure that the uniformity coefficient of the mixture is less than 5 percent.
The powder can be added into feed in an amount of 0.1-1.0% to effectively prevent cow mastitis.
Example 6 preparation of a granular formulation of a plant composition for preventing mastitis in dairy cows
The raw materials comprise: formulations A1-A5 of examples 1-5
The preparation method comprises the following steps: weighing the crushed plant materials according to the formula, mixing and sieving by a 100-mesh sieve; adding appropriate amount of dextrin and starch, adding 60% ethanol as humectant, sieving with 20 mesh sieve, and granulating; drying, grading, detecting, and packaging to obtain the final product.
The addition of 0.1-1.0% of the granules in the feed can effectively prevent the cow mastitis.
Under the condition that the dairy cow generates mastitis, the usage amount of the plant composition for preventing the dairy cow mastitis can be increased to 5-10 times of the usage amount of the powder, and the plant composition can be used as a veterinary drug and has a treatment effect.
EXAMPLE 7 preparation of another plant composition powder for prevention of mastitis in cows
The formulation of another botanical composition for preventing mastitis in dairy cows of the present invention is shown in table 2.
TABLE 2 formulation of another herbal composition for preventing mastitis in dairy cows
The other preparation method of the plant composition powder for preventing the cow mastitis comprises the following steps:
weighing each plant extract according to the formula B1-B5, uniformly mixing, and sieving with a 100-mesh sieve.
The powder can be added into feed in an amount of 0.02-0.2% to effectively prevent cow mastitis.
EXAMPLE 8 preparation of an alternative botanical composition aqua for the prevention of mastitis in dairy cows
The raw materials comprise: the formulations B1-B5 in example 7.
The preparation method comprises the following steps:
(1) weighing each plant extract according to the formula amount to make the total weight be 100 g;
(2) adding appropriate amount of water, stirring, adding appropriate amount of 5mol/L NaOH solution to dissolve the extract completely, and adjusting pH to 7.0.
(3) Weighing 3g of sodium benzoate, adding a proper amount of water, and stirring to completely dissolve the sodium benzoate;
(4) pouring the dissolved sodium benzoate solution into the extract solution, adding 8ml of Tween-80, adding water to 1000ml, stirring, standing at room temperature for 48h, centrifuging, filtering the supernatant with 0.45 μm microporous membrane, packaging, and sterilizing.
The application and dosage are as follows: the mixture is drunk, and each 1L of water is 0.5-2.5 ml.
EXAMPLE 9 preparation of Another herbal composition granule for preventing mastitis in milk cow
The raw materials comprise: the formulations B1-B5 in example 7.
The preparation method comprises the following steps: weighing the extracts according to the formula ratio, mixing and sieving with a 150-mesh sieve; adding appropriate amount of dextrin and starch, adding 60% ethanol as humectant, sieving with 20 mesh sieve, and granulating; drying, grading, detecting, and packaging to obtain the final product.
The application and dosage are as follows: dissolved in water for oral administration, 0.25-5 g per 1L of water.
Under the condition that the dairy cow generates mastitis, the usage amount of the plant composition for preventing the dairy cow mastitis can be increased to be 5-10 times of the usage amount of powder, and the plant composition is added into a feed to be used as a veterinary drug, so that a treatment effect can be achieved.
The beneficial effects of the botanical compositions for preventing mastitis in dairy cows of the present invention are illustrated by specific test examples below.
Test example 1
1 materials and methods
80 healthy cows with the number of fetuses, the number of lactation days and the average milk yield basically consistent and the average somatic cell number of about 45 ten thousand/mL are selected and randomly divided into a control group (TMR daily ration), a test group 1(TMR daily ration + 5 kg/ton of the formula of example 1A 1), a test group 2(TMR daily ration + 5 kg/ton of the formula of example 1A 3) and a test group 3(TMR daily ration + 5 kg/ton of the formula of example 1A 5) to carry out feeding tests, wherein the feeding tests are carried out for 3 days in advance and 30 days in formal test. Adopts a uniform feeding management mode, feeds for 3 times every day, and milks for 3 times. The feed is uniformly fed every day, and the waste is avoided.
2 results and analysis
TABLE 3 influence on milk yield of cows
TABLE 4 cow mastitis cases during the test
TABLE 5 prevention of effects of cow mastitis plant extract feed additives on cow somatic cell count
The experimental results are shown in tables 3-5, and the results show that: the composition has good effects on maintaining the milk yield of the dairy cow and reducing the somatic cell count of the dairy cow, and has a remarkable effect on controlling the mastitis of the dairy cow.
Test example 2
The experimental example researches the treatment effect of the injection, the aqua and the granule of the other plant composition for treating the cow mastitis on the cow mastitis.
72 Holstein cows with dominant mastitis were selected and randomly divided into control group, test group 1, test group 2 and test group 3, 10 cows each. No drug was administered to the control group; experimental group 1 was perfused using a breast pool: penicillin is 200 ten thousand units per time, streptomycin is 100 ten thousand units per time, 0.25% procaine hydrochloride is 10mL per time, and the mixture is diluted by 30mL of physiological saline for 3 times per day; test group 2 group drenched powder B3 prepared according to the invention in example 7, with an addition of 30 g/day; test group 3 drenched powder B5 prepared according to example 7 of the present invention, with an addition of 30 g/day; the administration is continued for 5 days.
TABLE 6 therapeutic effect on cow mastitis
The test results are shown in table 6, and the results show that the composition provided by the invention is used for observing and treating cow mastitis, wherein the cure rate is more than 88%, and the total effective rate is more than 94%.
Finally, it should be noted that the above-mentioned embodiments are only intended to illustrate the technical solutions of the present invention and should not be construed as limiting, and that various changes and modifications of the present invention may be made without departing from the spirit and scope of the present invention, which fall within the scope of the claimed invention.