AU2018220470B2 - Method and cell line for production of polyketides in yeast - Google Patents

Method and cell line for production of polyketides in yeast Download PDF

Info

Publication number
AU2018220470B2
AU2018220470B2 AU2018220470A AU2018220470A AU2018220470B2 AU 2018220470 B2 AU2018220470 B2 AU 2018220470B2 AU 2018220470 A AU2018220470 A AU 2018220470A AU 2018220470 A AU2018220470 A AU 2018220470A AU 2018220470 B2 AU2018220470 B2 AU 2018220470B2
Authority
AU
Australia
Prior art keywords
yeast cell
polyketide
olivetol
dipks
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2018220470A
Other languages
English (en)
Other versions
AU2018220470A1 (en
Inventor
Alexander James CAMPBELL
Kevin John CHEN
Shoham MOOKERJEE
Zachary Douglas WILTSHIRE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hyasynth Biologicals Inc
Original Assignee
Hyasynth Biologicals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hyasynth Biologicals Inc filed Critical Hyasynth Biologicals Inc
Publication of AU2018220470A1 publication Critical patent/AU2018220470A1/en
Application granted granted Critical
Publication of AU2018220470B2 publication Critical patent/AU2018220470B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C39/00—Compounds having at least one hydroxy or O-metal group bound to a carbon atom of a six-membered aromatic ring
    • C07C39/02—Compounds having at least one hydroxy or O-metal group bound to a carbon atom of a six-membered aromatic ring monocyclic with no unsaturation outside the aromatic ring
    • C07C39/08—Dihydroxy benzenes; Alkylated derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C65/00—Compounds having carboxyl groups bound to carbon atoms of six—membered aromatic rings and containing any of the groups OH, O—metal, —CHO, keto, ether, groups, groups, or groups
    • C07C65/01—Compounds having carboxyl groups bound to carbon atoms of six—membered aromatic rings and containing any of the groups OH, O—metal, —CHO, keto, ether, groups, groups, or groups containing hydroxy or O-metal groups
    • C07C65/03—Compounds having carboxyl groups bound to carbon atoms of six—membered aromatic rings and containing any of the groups OH, O—metal, —CHO, keto, ether, groups, groups, or groups containing hydroxy or O-metal groups monocyclic and having all hydroxy or O-metal groups bound to the ring
    • C07C65/05—Compounds having carboxyl groups bound to carbon atoms of six—membered aromatic rings and containing any of the groups OH, O—metal, —CHO, keto, ether, groups, groups, or groups containing hydroxy or O-metal groups monocyclic and having all hydroxy or O-metal groups bound to the ring o-Hydroxy carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1288—Transferases for other substituted phosphate groups (2.7.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/22—Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01041—Phosphoglycerol geranylgeranyltransferase (2.5.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/08—Transferases for other substituted phosphate groups (2.7.8)
    • C12Y207/08007—Holo-[acyl-carrier-protein] synthase (2.7.8.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y602/00—Ligases forming carbon-sulfur bonds (6.2)
    • C12Y602/01—Acid-Thiol Ligases (6.2.1)
    • C12Y602/01001—Acetate-CoA ligase (6.2.1.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Furan Compounds (AREA)
  • Pyrane Compounds (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
AU2018220470A 2017-02-17 2018-02-19 Method and cell line for production of polyketides in yeast Active AU2018220470B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762460526P 2017-02-17 2017-02-17
US62/460,526 2017-02-17
PCT/CA2018/050190 WO2018148849A1 (en) 2017-02-17 2018-02-19 Method and cell line for production of polyketides in yeast

Publications (2)

Publication Number Publication Date
AU2018220470A1 AU2018220470A1 (en) 2019-09-05
AU2018220470B2 true AU2018220470B2 (en) 2022-02-24

Family

ID=63169138

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2018220470A Active AU2018220470B2 (en) 2017-02-17 2018-02-19 Method and cell line for production of polyketides in yeast
AU2018220469A Active AU2018220469B2 (en) 2017-02-17 2018-02-19 Method and cell line for production of phytocannabinoids and phytocannabinoid analogues in yeast

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2018220469A Active AU2018220469B2 (en) 2017-02-17 2018-02-19 Method and cell line for production of phytocannabinoids and phytocannabinoid analogues in yeast

Country Status (11)

Country Link
US (3) US11078502B2 (https=)
EP (2) EP3583222A4 (https=)
JP (2) JP2020507351A (https=)
KR (2) KR20200004784A (https=)
CN (2) CN110651047B (https=)
AU (2) AU2018220470B2 (https=)
CA (2) CA3054423A1 (https=)
IL (2) IL268725B2 (https=)
MX (2) MX2019009708A (https=)
SG (2) SG11201907504PA (https=)
WO (2) WO2018148848A1 (https=)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110914416B (zh) 2017-04-27 2023-07-21 加州大学董事会 产生大麻素和大麻素衍生物的微生物和方法
AU2018346434A1 (en) * 2017-10-05 2020-05-14 Intrexon Corporation Microorganisms and methods for the fermentation of cannabinoids
WO2019190945A1 (en) * 2018-03-29 2019-10-03 William Marsh Rice University Biosynthesis of olivetolic acid
AU2019262112A1 (en) 2018-04-30 2020-11-19 Algae-C Inc. Engineered microorganism for the production of cannabinoid biosynthetic pathway products
WO2020060948A1 (en) 2018-09-17 2020-03-26 Levadura Biotechnology, Inc. Production of cannabinoids in yeast using a fatty acid feedstock
IL326299A (en) * 2018-10-31 2026-04-01 Baymedica Inc Cannabinoid analogs and methods for their preparation
CN113227353A (zh) * 2018-11-14 2021-08-06 马努斯生物合成股份有限公司 用于产生大麻素的微生物细胞及方法
MY205459A (en) * 2018-12-06 2024-10-22 Cytomx Therapeutics Inc Matrix metalloprotease-cleavable and serine or cysteine protease-cleavable substrates and methods of use thereof
US20220127649A1 (en) * 2019-01-30 2022-04-28 Genomatica, Inc. Engineered cells for improved production of cannabinoids
EP3917642A4 (en) 2019-01-30 2023-04-05 Genomatica, Inc. RECOVERY, DECARBOXYLATION AND PURIFICATION OF CANNABINOIDS FROM GMO CULTURES
CA3130763A1 (en) 2019-02-25 2020-09-03 Ginkgo Bioworks, Inc. Biosynthesis of cannabinoids and cannabinoid precursors
US20220186267A1 (en) * 2019-03-27 2022-06-16 Rynetech Bio, Inc. Biosynthetic Cannabidiol Production In Engineered Microorganisms
SG11202112690YA (en) * 2019-05-22 2021-12-30 Hyasynth Biologicals Inc Methods and cells for production of phytocannabinoids and phytocannabinoid precursors
ES3037766T3 (en) 2019-06-11 2025-10-07 Symrise Ag Synthesis and purification of cannabigerol and its application
CA3151799A1 (en) * 2019-08-18 2021-02-25 Ginkgo Bioworks, Inc. Biosynthesis of cannabinoids and cannabinoid precursors
EP4025705A4 (en) * 2019-09-06 2024-01-17 Genomatica, Inc. PRENYL TRANSFERASE VARIANTS AND METHOD FOR PRODUCING PRENYLATED AROMATIC COMPOUNDS
CN120555521A (zh) * 2019-10-01 2025-08-29 杭州恩和生物科技有限公司 用于大麻素合成的酶和制备大麻素的方法
US20240084337A1 (en) * 2019-10-11 2024-03-14 National University Of Singapore Biosynthesis of cannabinoid precursors using novel aromatic prenyl transferases
KR20220079578A (ko) 2019-10-11 2022-06-13 내셔널 유니버시티 오브 싱가포르 신규 칸나비노이드 신타아제를 사용한 칸나비게롤산으로부터 칸나비노이드의 생합성
MX2022008463A (es) 2020-01-10 2022-10-18 Univ California Plataforma biosintética para la producción de ácido olivetólico y análogos del ácido olivetólico.
US20250346871A1 (en) * 2020-03-06 2025-11-13 Cellibre, Inc. Prenyltransferases and methods of making and use thereof
EP4126916A2 (en) 2020-03-27 2023-02-08 Willow Biosciences Inc. Compositions and methods for recombinant biosynthesis of cannabinoids
US12529077B2 (en) * 2020-04-08 2026-01-20 Københavns Universitet Production of geranyl diphosphate-derived compounds
EP3901256A1 (en) * 2020-04-21 2021-10-27 Synbionik GmbH Optimized production of cbga from olivetol acid and geranyl pyrophosphate via synnphb
CA3175683A1 (en) 2020-04-29 2021-11-04 Xueyang FENG Compositions and methods for enhancing recombinant biosynthesis of cannabinoids
WO2021225952A1 (en) * 2020-05-08 2021-11-11 Lygos, Inc. Large scale production of olivetol, olivetolic acid and other alkyl resorcinols by fermentation
EP4176054A1 (en) * 2020-07-03 2023-05-10 Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e.V. -Hans-Knöll-Institut- (HKI) Method for producing olivetolic acid in an amoebozoa host species
KR20230042073A (ko) * 2020-07-24 2023-03-27 히야신스 바이오로지컬스 아이엔씨. 치환된 칸나비노이드 및 전구체를 제조하기 위해 변형 효소를 이용하는 방법 및 이를 함유하는 세포
CN111979134B (zh) 2020-08-28 2022-02-15 江南大学 一种合成胭脂红酸的重组酿酒酵母的构建及其应用
CN112042677B (zh) * 2020-09-21 2021-09-14 中国农业科学院麻类研究所 一种大麻叶提取物及其制备方法、用途
US11427840B2 (en) 2020-11-20 2022-08-30 Hyasynth Biologicals Inc. Olivetolic acid cyclase variants with improved activity for use in production of phytocannabinoids
CA3199891A1 (en) * 2020-11-24 2022-06-02 Ruth Gallily Cannabinoid derivatives and their use for the treatment of inflammation and/or pain and/or obesity
US20240026314A1 (en) * 2020-11-25 2024-01-25 Steven Rothstein Polypeptides for use in the synthesis of bioactive phenolic compounds
WO2022125960A1 (en) 2020-12-11 2022-06-16 Willow Biosciences, Inc. Recombinant acyl activating enzyme (aae) genes for enhanced biosynthesis of cannabinoids and cannabinoid precursors
EP4347800A1 (en) 2021-05-26 2024-04-10 Invizyne Technologies, Inc. Prenyltransferase variants with increased thermostability
WO2023010083A2 (en) 2021-07-30 2023-02-02 Willow Biosciences, Inc. Recombinant prenyltransferase polypeptides engineered for enhanced biosynthesis of cannabinoids
CA3227236A1 (en) 2021-08-19 2023-02-23 Trish Choudhary Recombinant olivetolic acid cyclase polypeptides engineered for enhanced biosynthesis of cannabinoids
CN113881584B (zh) * 2021-09-16 2023-06-27 广东省科学院微生物研究所(广东省微生物分析检测中心) 一株经航天育种技术选育的酿酒酵母及其突变位点应用
WO2023056350A1 (en) * 2021-09-29 2023-04-06 Ginkgo Bioworks, Inc. Biosynthesis of cannabinoids and cannabinoid precursors
WO2023069921A1 (en) 2021-10-19 2023-04-27 Epimeron Usa, Inc. Recombinant thca synthase polypeptides engineered for enhanced biosynthesis of cannabinoids
CN114621982B (zh) * 2022-03-16 2023-11-07 嘉兴欣贝莱生物科技有限公司 香叶基二磷酸酯的生物合成方法及其在制备大麻类化合物中的应用
CN116024111B (zh) * 2022-12-03 2025-09-05 中国科学院深圳先进技术研究院 一种产大麻萜酚的微生物细胞及其构建方法与应用
CN119685467A (zh) * 2023-12-01 2025-03-25 上海市东方医院（同济大学附属东方医院） 诊断和治疗阿尔茨海默病的医药制剂
CN119753053A (zh) * 2025-03-07 2025-04-04 中国农业科学院植物保护研究所 磷酸泛酰巯基乙胺基转移酶3x32、其编码基因、含有该基因的重组载体和重组菌的应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120122180A1 (en) * 2006-09-14 2012-05-17 Salk Institute For Biological Studies Incorporation of type iii polyketide synthases into multidomain proteins of the type i and iii polyketide synthase and fatty acid synthase families

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE455785T1 (de) 2000-11-02 2010-02-15 Ajinomoto Kk Neue pyrazolderivate und diese enthaltende mittel gegen diabetes
US7238514B2 (en) 2001-01-05 2007-07-03 William Marsh Rice University Diterpene-producing unicellular organism
EP1338608A3 (en) * 2001-12-20 2004-05-06 Cellzome Ag Protein complexes and methods for their use
AU2002367554A1 (en) 2001-12-20 2003-09-09 Cellzome Ag Protein complexes and methods for their use
US7186519B2 (en) 2002-11-13 2007-03-06 Trustees Of Dartmouth College Method for detecting intracellular cholesterol
WO2006060839A2 (en) 2004-12-10 2006-06-15 Universität Für Bodenkultur Wien Method for identifying ppt inhibitors
US7361483B2 (en) 2005-01-28 2008-04-22 The Salk Institute For Biological Studies Aromatic prenyltransferases, nucleic acids encoding same and uses therefor
CA2770774C (en) 2009-08-12 2020-07-14 National Research Council Of Canada Aromatic prenyltransferase from cannabis
WO2011127569A1 (en) 2010-04-14 2011-10-20 Voiceage Corporation Flexible and scalable combined innovation codebook for use in celp coder and decoder
CA3130705A1 (en) 2010-04-15 2011-10-20 National Research Council Of Canada Genes and proteins for aromatic polyketide synthesis
EP2601292B1 (en) 2010-08-06 2018-04-18 Biopetrolia AB Methods and products for production of wax esters
CN103562398A (zh) 2011-03-22 2014-02-05 Opx生物工艺学公司 化学产品的微生物生产以及相关的组合物、方法和系统
WO2013006853A1 (en) 2011-07-07 2013-01-10 Cidra Corporate Services Inc. Spectroscopic analysis of oil sands ore face for real time ore blend management
AU2012283708B2 (en) * 2011-07-13 2017-02-23 National Research Council Of Canada Genes and proteins for alkanoyl-CoA synthesis
BR112014010448A2 (pt) * 2011-11-02 2017-04-18 Genomatica Inc microorganismos e métodos para a produção de caprolactona
US9670494B2 (en) 2012-01-06 2017-06-06 Firmenich Sa Genetically engineered yeast cells
WO2014018982A1 (en) * 2012-07-27 2014-01-30 The Regents Of The University Of California Systems and methods for enhancing gene expression
WO2014134281A1 (en) 2013-02-28 2014-09-04 Full Spectrum Laboratories Limited Biosynthesis of cannabinoids
EP3008178A1 (en) 2013-06-14 2016-04-20 Technical University of Denmark Microbial production of 3-hydroxypropionic acid
WO2014207113A1 (en) 2013-06-26 2014-12-31 Abengoa Bioenergia Nuevas Tecnologias S.A. Yeasts engineered for the production of valuable chemicals from sugars
US20160230200A1 (en) 2013-09-05 2016-08-11 Danmarks Tekniske Universitet Microorganisms for efficient production of melatonin and related compounds
US9822384B2 (en) 2014-07-14 2017-11-21 Librede Inc. Production of cannabinoids in yeast
US9394510B2 (en) 2014-08-25 2016-07-19 Full Spectrum Laboratories Limited Apparatus and methods for the simultaneous production of compounds
DK3277827T3 (da) 2015-04-02 2021-06-14 Biopetrolia Ab Svampeceller og fremgangsmåder til fremstilling af produkter afledt af meget langkædede fedtsyrer
WO2017139496A1 (en) 2016-02-09 2017-08-17 Cevolva Biotech, Inc. Microbial engineering for the production of cannabinoids and cannabinoid precursors
EP3430151A4 (en) * 2016-03-16 2020-09-02 William Marsh Rice University MICROBIAL SYNTHESIS OF ISOPRENOID, ISOPRENOID AND DERIVATIVE PRECURSORS INCLUDING PRENYLATED AROMATIC COMPOUNDS
CN110914416B (zh) 2017-04-27 2023-07-21 加州大学董事会 产生大麻素和大麻素衍生物的微生物和方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120122180A1 (en) * 2006-09-14 2012-05-17 Salk Institute For Biological Studies Incorporation of type iii polyketide synthases into multidomain proteins of the type i and iii polyketide synthase and fatty acid synthase families

Also Published As

Publication number Publication date
IL268723A (en) 2019-10-31
JP2020507352A (ja) 2020-03-12
JP2020507351A (ja) 2020-03-12
EP3583222A1 (en) 2019-12-25
EP3583217A1 (en) 2019-12-25
IL268723B2 (en) 2025-04-01
IL268725A (en) 2019-10-31
JP7162018B6 (ja) 2024-02-02
KR20200004784A (ko) 2020-01-14
AU2018220469B2 (en) 2023-03-09
SG11201907469YA (en) 2019-09-27
US20200283807A1 (en) 2020-09-10
EP3583222A4 (en) 2021-01-13
IL268725B1 (en) 2024-08-01
US11312979B2 (en) 2022-04-26
MX2019009708A (es) 2020-02-07
KR20190141653A (ko) 2019-12-24
WO2018148849A1 (en) 2018-08-23
US11078502B2 (en) 2021-08-03
CA3054423A1 (en) 2018-08-23
AU2018220469A1 (en) 2019-09-05
CN110651047A (zh) 2020-01-03
CA3054152A1 (en) 2018-08-23
SG11201907504PA (en) 2019-09-27
JP7162018B2 (ja) 2022-10-27
IL268723B1 (en) 2024-12-01
CN110637088B (zh) 2024-03-22
IL268725B2 (en) 2024-12-01
US20190367953A1 (en) 2019-12-05
CN110637088A (zh) 2019-12-31
CN110651047B (zh) 2024-01-05
KR102589523B1 (ko) 2023-10-17
US20200239916A1 (en) 2020-07-30
US10975395B2 (en) 2021-04-13
MX2019009712A (es) 2020-02-07
WO2018148848A1 (en) 2018-08-23
EP3583217A4 (en) 2021-04-07
AU2018220470A1 (en) 2019-09-05

Similar Documents

Publication Publication Date Title
AU2018220470B2 (en) Method and cell line for production of polyketides in yeast
DK2324120T3 (en) Manipulating SNF1 protein kinase OF REVISION OF OIL CONTENT IN OLEAGINOUS ORGANISMS
CN101939434B (zh) 用于在大豆中提高种子贮藏油脂的生成和改变脂肪酸谱的来自解脂耶氏酵母的dgat基因
DK2831238T3 (en) CLONING PROCEDURE
CN101365788B (zh) Δ-9延伸酶及其在制备多不饱和脂肪酸中的用途
CN108138121B (zh) 用微生物高水平生产长链二羧酸
TW202536186A (zh) 治療杜興氏(duchenne)肌肉失養症之大型突變熱點之aav載體介導之刪除
KR102253532B1 (ko) 낮은 ph에서 발효에 의한 유기산의 제조
KR20130032897A (ko) 알코올 발효 시의 알코올 에스테르의 생성 및 원위치에서의 생성물 제거
KR20070085669A (ko) 고농도의 아라키돈산을 생성하는 야로위아 리폴리티카 균주
CN109661403A (zh) 前导序列修饰的葡糖淀粉酶多肽和具有增强的生物产物产生的工程化的酵母菌株
KR20140092759A (ko) 숙주 세포 및 아이소부탄올의 제조 방법
CN108473968A (zh) 生产植物鞘氨醇或二氢神经鞘氨醇的方法
KR20130138760A (ko) 고농도의 에이코사펜타엔산 생성을 위한 재조합 미생물 숙주 세포
KR20230156365A (ko) 메타게놈 유래의 새로운 CRISPR-Cas 뉴클레아제
DK2475679T3 (da) Forbedrede, optimerede stammer af yarrowia lipolytica til fremstilling af højkoncentreret eicosapentaensyre
DK2609201T3 (en) Mutant DELTA-9 elongases AND USE THEREOF FOR THE PRODUCTION OF polyunsaturated fatty acids
AU2022331118A1 (en) Burden-addicted production strains
US6696621B2 (en) Selectable marker in plants

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)