AU2015280120B2 - Nuclease-mediated DNA assembly - Google Patents

Nuclease-mediated DNA assembly Download PDF

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Publication number
AU2015280120B2
AU2015280120B2 AU2015280120A AU2015280120A AU2015280120B2 AU 2015280120 B2 AU2015280120 B2 AU 2015280120B2 AU 2015280120 A AU2015280120 A AU 2015280120A AU 2015280120 A AU2015280120 A AU 2015280120A AU 2015280120 B2 AU2015280120 B2 AU 2015280120B2
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nucleic acid
sequence
complementary
dna
digested
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AU2015280120A1 (en
Inventor
Ka-Man Venus Lai
Lynn Macdonald
John Mcwhirter
Corey MOMONT
Caitlin MONTAGNA
Andrew J. Murphy
Jose F. Rojas
Chris SCHOENHERR
David M. Valenzuela
Gregg S. Warshaw
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Regeneron Pharmaceuticals Inc
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Regeneron Pharmaceuticals Inc
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Priority to AU2017213564A priority Critical patent/AU2017213564B2/en
Priority to AU2019200094A priority patent/AU2019200094B2/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AU2015280120A 2014-06-23 2015-06-23 Nuclease-mediated DNA assembly Active AU2015280120B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2017213564A AU2017213564B2 (en) 2014-06-23 2017-08-11 Nuclease-mediated dna assembly
AU2019200094A AU2019200094B2 (en) 2014-06-23 2019-01-08 Nuclease-mediated dna assembly

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201462015809P 2014-06-23 2014-06-23
US62/015,809 2014-06-23
US201462016400P 2014-06-24 2014-06-24
US62/016,400 2014-06-24
US201462036983P 2014-08-13 2014-08-13
US62/036,983 2014-08-13
PCT/US2015/037199 WO2015200334A1 (en) 2014-06-23 2015-06-23 Nuclease-mediated dna assembly

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AU2017213564A Division AU2017213564B2 (en) 2014-06-23 2017-08-11 Nuclease-mediated dna assembly

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AU2015280120A1 AU2015280120A1 (en) 2017-01-19
AU2015280120B2 true AU2015280120B2 (en) 2017-05-25

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AU2015280120A Active AU2015280120B2 (en) 2014-06-23 2015-06-23 Nuclease-mediated DNA assembly
AU2017213564A Active AU2017213564B2 (en) 2014-06-23 2017-08-11 Nuclease-mediated dna assembly
AU2019200094A Active AU2019200094B2 (en) 2014-06-23 2019-01-08 Nuclease-mediated dna assembly

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AU2019200094A Active AU2019200094B2 (en) 2014-06-23 2019-01-08 Nuclease-mediated dna assembly

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US (6) US9738897B2 (https=)
EP (3) EP3708663A1 (https=)
JP (4) JP6336140B2 (https=)
KR (2) KR101822902B1 (https=)
CN (2) CN112029787B (https=)
AU (3) AU2015280120B2 (https=)
BR (1) BR112016030145A8 (https=)
CA (1) CA2953499C (https=)
CY (2) CY1120520T1 (https=)
DK (2) DK3155099T3 (https=)
ES (2) ES2781323T3 (https=)
HR (1) HRP20200523T1 (https=)
HU (1) HUE049405T2 (https=)
IL (1) IL249612B (https=)
LT (1) LT3354732T (https=)
MX (2) MX384887B (https=)
NZ (1) NZ765591A (https=)
PL (2) PL3354732T3 (https=)
PT (2) PT3155099T (https=)
RS (1) RS60366B1 (https=)
RU (1) RU2707911C2 (https=)
SG (2) SG10201803444YA (https=)
SI (1) SI3354732T1 (https=)
SM (1) SMT202000174T1 (https=)
WO (1) WO2015200334A1 (https=)

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