AU2015225439B2 - Enhanced production of recombinant CRM197 in E. coli - Google Patents

Enhanced production of recombinant CRM197 in E. coli Download PDF

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Publication number
AU2015225439B2
AU2015225439B2 AU2015225439A AU2015225439A AU2015225439B2 AU 2015225439 B2 AU2015225439 B2 AU 2015225439B2 AU 2015225439 A AU2015225439 A AU 2015225439A AU 2015225439 A AU2015225439 A AU 2015225439A AU 2015225439 B2 AU2015225439 B2 AU 2015225439B2
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crm197
coli
strain
reduced genome
protein
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AU2015225439A1 (en
Inventor
Cristopher R. Blattner
Frederick R. Blattner
Hyunsic Choi
David A. Frisch
Terrance M. Henker
Charles F. Landry
Robert E. Novy
Gyorgy Posfai
Eric A. Steffen
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Scarab Genomics LLC
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Scarab Genomics LLC
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Priority to AU2021201686A priority Critical patent/AU2021201686A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08—Reducing the nucleic acid content
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AU2015225439A 2014-03-03 2015-03-02 Enhanced production of recombinant CRM197 in E. coli Active AU2015225439B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2021201686A AU2021201686A1 (en) 2014-03-03 2021-03-17 Enhanced Production Of Recombinant CRM197 In E.Coli

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461947234P 2014-03-03 2014-03-03
US61/947,234 2014-03-03
PCT/US2015/018338 WO2015134402A1 (en) 2014-03-03 2015-03-02 Enhanced production of recombinant crm197 in e. coli

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2021201686A Division AU2021201686A1 (en) 2014-03-03 2021-03-17 Enhanced Production Of Recombinant CRM197 In E.Coli

Publications (2)

Publication Number Publication Date
AU2015225439A1 AU2015225439A1 (en) 2016-09-01
AU2015225439B2 true AU2015225439B2 (en) 2020-12-17

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
AU2015225439A Active AU2015225439B2 (en) 2014-03-03 2015-03-02 Enhanced production of recombinant CRM197 in E. coli
AU2021201686A Abandoned AU2021201686A1 (en) 2014-03-03 2021-03-17 Enhanced Production Of Recombinant CRM197 In E.Coli

Family Applications After (1)

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AU2021201686A Abandoned AU2021201686A1 (en) 2014-03-03 2021-03-17 Enhanced Production Of Recombinant CRM197 In E.Coli

Country Status (8)

Country Link
US (1) US10479820B2 (enExample)
EP (1) EP3113800B1 (enExample)
JP (2) JP6473460B2 (enExample)
KR (1) KR102315246B1 (enExample)
CN (1) CN106456769B (enExample)
AU (2) AU2015225439B2 (enExample)
CA (1) CA2940316C (enExample)
WO (1) WO2015134402A1 (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6545676B2 (ja) * 2013-11-14 2019-07-17 スカラブ ゲノミクス， エルエルシー 改善された代謝能力を有する細菌
EP3221339B1 (en) 2014-11-20 2024-08-21 Biological E Limited Codon optimized polynucleotide for high level expression of crm197
AU2016242996B2 (en) * 2015-04-02 2021-02-18 Scarab Genomics, Llc Materials and methods for extended continuous flow fermentation of reduced genome bacteria
KR101908590B1 (ko) * 2017-02-01 2018-10-16 (주)포바이오코리아 Crm197의 용해성 단백질 발현 및 정제 방법
ES2926535T3 (es) * 2017-04-22 2022-10-26 Biological E Ltd Método mejorado para la producción de alto nivel de CRM
EP3444269A1 (en) 2017-08-17 2019-02-20 National Research Council of Canada Systems and methods for the production of diphtheria toxin polypeptides
US20200331973A1 (en) * 2017-09-01 2020-10-22 Lorven Biologics Private Limited Nucleic acid encoding crm197 and process for improved expression thereof
AU2018329671B2 (en) * 2017-09-05 2023-12-07 Scarab Genomics, Llc Methods and compositions for improved expression of recombinant proteins
KR20200111685A (ko) * 2018-01-19 2020-09-29 오비아이 파머 인코퍼레이티드 Crm197 단백질 발현
WO2019178587A1 (en) * 2018-03-16 2019-09-19 Bio-Rad Laboratories, Inc. Purification of target molecules
KR102048456B1 (ko) 2018-03-28 2019-11-25 강원대학교 산학협력단 재조합 대장균을 이용한 rCRM197 생산방법
CN109402035A (zh) * 2018-11-06 2019-03-01 华中农业大学 一种肠外致病性大肠杆菌重组菌株及其应用
KR102099342B1 (ko) * 2019-09-03 2020-04-10 주식회사 제노포커스 Crm197 단백질 발현 방법
NL2023863B1 (en) * 2019-09-20 2021-05-25 Univ Griffith Protein particles and uses thereof
KR102142255B1 (ko) * 2019-12-05 2020-08-07 주식회사 제노포커스 Crm197 단백질 발현 방법
BE1029145B1 (fr) 2021-02-26 2022-09-27 Curavac Europe Methode de production d'une forme periplasmique de la proteine crm197
BE1029146B1 (fr) * 2021-02-26 2022-09-26 Xpress Biologics Procede de purification d'une proteine d'interet
CN119552230A (zh) * 2024-12-12 2025-03-04 中国人民解放军军事科学院军事医学研究院 一种脂肪酸化修饰的白喉毒素抗原及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120289688A1 (en) * 2009-10-08 2012-11-15 Normand Blais Expression system
US20130011874A1 (en) * 2005-08-20 2013-01-10 Scarab Genomics, Llc Reduced genome e. coli

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6989265B2 (en) * 2002-01-23 2006-01-24 Wisconsin Alumni Research Foundation Bacteria with reduced genome
US7300776B2 (en) * 2004-04-26 2007-11-27 Ajinomoto Co., Inc. L-amino acid-producing bacterium and a method for producing L-amino acid
JP2009165355A (ja) * 2006-04-28 2009-07-30 Ajinomoto Co Inc Ｌ−アミノ酸を生産する微生物及びｌ−アミノ酸の製造法
JP5506149B2 (ja) * 2007-11-22 2014-05-28 住友化学株式会社 昆虫由来のコリンアセチルトランスフェラーゼ活性に関わる有害生物の生理状態に変化を与える薬剤
US8367380B2 (en) * 2008-02-22 2013-02-05 Scarab Genomics Llc Compositions and methods for amino acid biosynthesis
DE102008063900A1 (de) * 2008-12-19 2010-06-24 Wacker Chemie Ag Verfahren zur fermentativen Herstellung von heterologen Proteinen mittels Escherichia coli
IT1398927B1 (it) * 2009-06-25 2013-03-28 Consorzio Interuniversitario Per Lo Sviluppo Dei Sistemi A Grande Interfase Csgi Espressione batterica di un gene artificiale per la produzione di crm197 e derivati.
US8546125B2 (en) * 2011-02-21 2013-10-01 Tianjin Greenbio Material Co., Ltd. Recombinant hosts and methods for manufacturing polyhydroxyalkanoate
WO2013059595A1 (en) * 2011-10-20 2013-04-25 Scarab Genomics, Llc Reduced genome bacteria with improved genetic stability
US20160251609A1 (en) * 2011-10-20 2016-09-01 Scarab Genomics, Llc Reduced Genome Bacteria With Improved Genetic Stability

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130011874A1 (en) * 2005-08-20 2013-01-10 Scarab Genomics, Llc Reduced genome e. coli
US20120289688A1 (en) * 2009-10-08 2012-11-15 Normand Blais Expression system

Also Published As

Publication number Publication date
JP2019058195A (ja) 2019-04-18
EP3113800B1 (en) 2024-06-19
JP6636661B2 (ja) 2020-01-29
EP3113800A1 (en) 2017-01-11
EP3113800A4 (en) 2017-10-18
CA2940316C (en) 2022-03-15
CN106456769B (zh) 2020-04-17
US20170073379A1 (en) 2017-03-16
US10479820B2 (en) 2019-11-19
WO2015134402A1 (en) 2015-09-11
AU2021201686A1 (en) 2021-04-08
CN106456769A (zh) 2017-02-22
JP2017508454A (ja) 2017-03-30
JP6473460B2 (ja) 2019-02-20
KR20160128362A (ko) 2016-11-07
AU2015225439A1 (en) 2016-09-01
CA2940316A1 (en) 2015-09-11
KR102315246B1 (ko) 2021-10-21

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