KR102315246B1 - 대장균에서 재조합 ｃｒｍ197의 향상된 생산 - Google Patents

대장균에서 재조합 ｃｒｍ197의 향상된 생산 Download PDF

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KR102315246B1
KR102315246B1 KR1020167026716A KR20167026716A KR102315246B1 KR 102315246 B1 KR102315246 B1 KR 102315246B1 KR 1020167026716 A KR1020167026716 A KR 1020167026716A KR 20167026716 A KR20167026716 A KR 20167026716A KR 102315246 B1 KR102315246 B1 KR 102315246B1
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crm197
coli
strain
reduced genome
expression
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KR20160128362A (ko
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크리스토퍼 알. 블래트너
데이비드 에이. 프리쉬
로버트 이. 노비
테런스 엠. 핸커
에릭 에이. 스테펜
프레드릭 알. 블래트너
현식 최
기요르기 포스파이
카를로스 에프. 랜드리
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스캐랩 게노믹스, 엘엘씨
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08—Reducing the nucleic acid content
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
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  • Microbiology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020167026716A 2014-03-03 2015-03-02 대장균에서 재조합 ｃｒｍ197의 향상된 생산 Active KR102315246B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461947234P 2014-03-03 2014-03-03
US61/947,234 2014-03-03
PCT/US2015/018338 WO2015134402A1 (en) 2014-03-03 2015-03-02 Enhanced production of recombinant crm197 in e. coli

Publications (2)

Publication Number Publication Date
KR20160128362A KR20160128362A (ko) 2016-11-07
KR102315246B1 true KR102315246B1 (ko) 2021-10-21

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KR1020167026716A Active KR102315246B1 (ko) 2014-03-03 2015-03-02 대장균에서 재조합 ｃｒｍ197의 향상된 생산

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Country Link
US (1) US10479820B2 (enExample)
EP (1) EP3113800B1 (enExample)
JP (2) JP6473460B2 (enExample)
KR (1) KR102315246B1 (enExample)
CN (1) CN106456769B (enExample)
AU (2) AU2015225439B2 (enExample)
CA (1) CA2940316C (enExample)
WO (1) WO2015134402A1 (enExample)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3068887B1 (en) * 2013-11-14 2019-10-30 Scarab Genomics LLC Bacteria with improved metabolic capacity
CA2968491C (en) 2014-11-20 2023-09-26 Biological E Limited Codon optimized polynucleotide for high level expression of crm197
CA2981583C (en) * 2015-04-02 2022-11-15 Scarab Genomics, Llc Continuous fermentation process for production of biological product using reduced genome escherichia coli
KR101908590B1 (ko) * 2017-02-01 2018-10-16 (주)포바이오코리아 Crm197의 용해성 단백질 발현 및 정제 방법
WO2018193475A2 (en) * 2017-04-22 2018-10-25 Biological E Limited An improved method for high level production of crm
EP3444269A1 (en) 2017-08-17 2019-02-20 National Research Council of Canada Systems and methods for the production of diphtheria toxin polypeptides
US20200331973A1 (en) * 2017-09-01 2020-10-22 Lorven Biologics Private Limited Nucleic acid encoding crm197 and process for improved expression thereof
WO2019050872A1 (en) 2017-09-05 2019-03-14 Scarab Genomics, Llc METHODS AND COMPOSITIONS FOR ENHANCED EXPRESSION OF RECOMBINANT PROTEINS
WO2019143911A1 (en) * 2018-01-19 2019-07-25 Obi Pharma, Inc. Crm197 protein expression
WO2019178587A1 (en) * 2018-03-16 2019-09-19 Bio-Rad Laboratories, Inc. Purification of target molecules
KR102048456B1 (ko) 2018-03-28 2019-11-25 강원대학교 산학협력단 재조합 대장균을 이용한 rCRM197 생산방법
CN109402035A (zh) * 2018-11-06 2019-03-01 华中农业大学 一种肠外致病性大肠杆菌重组菌株及其应用
KR102099342B1 (ko) * 2019-09-03 2020-04-10 주식회사 제노포커스 Crm197 단백질 발현 방법
NL2023863B1 (en) * 2019-09-20 2021-05-25 Univ Griffith Protein particles and uses thereof
KR102142255B1 (ko) * 2019-12-05 2020-08-07 주식회사 제노포커스 Crm197 단백질 발현 방법
BE1029146B1 (fr) * 2021-02-26 2022-09-26 Xpress Biologics Procede de purification d'une proteine d'interet
BE1029145B1 (fr) 2021-02-26 2022-09-27 Curavac Europe Methode de production d'une forme periplasmique de la proteine crm197
CN115850524A (zh) * 2022-12-29 2023-03-28 北京吉诺卫生物科技有限公司 一种生产白喉毒素无毒突变蛋白的方法及其使用的特异dna分子
CN119552230B (zh) * 2024-12-12 2026-03-13 中国人民解放军军事科学院军事医学研究院 一种脂肪酸化修饰的白喉毒素抗原及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003070880A2 (en) 2002-01-23 2003-08-28 Wisconsin Alumni Research Foundation Bacteria with reduced genome
WO2007024756A2 (en) 2005-08-20 2007-03-01 Scarab Genomics, Llc Reduced genome e. coli
JP2011512796A (ja) 2008-02-22 2011-04-28 スカラブ ジェノミクス リミティド ライアビリティ カンパニー アミノ酸生合成のための組成物及び方法
JP2013507113A (ja) 2009-10-08 2013-03-04 グラクソスミスクライン バイオロジカルズ ソシエテ アノニム 発現系

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US7300776B2 (en) * 2004-04-26 2007-11-27 Ajinomoto Co., Inc. L-amino acid-producing bacterium and a method for producing L-amino acid
JP2009165355A (ja) * 2006-04-28 2009-07-30 Ajinomoto Co Inc Ｌ−アミノ酸を生産する微生物及びｌ−アミノ酸の製造法
JP5506149B2 (ja) * 2007-11-22 2014-05-28 住友化学株式会社 昆虫由来のコリンアセチルトランスフェラーゼ活性に関わる有害生物の生理状態に変化を与える薬剤
DE102008063900A1 (de) * 2008-12-19 2010-06-24 Wacker Chemie Ag Verfahren zur fermentativen Herstellung von heterologen Proteinen mittels Escherichia coli
IT1398927B1 (it) * 2009-06-25 2013-03-28 Consorzio Interuniversitario Per Lo Sviluppo Dei Sistemi A Grande Interfase Csgi Espressione batterica di un gene artificiale per la produzione di crm197 e derivati.
US8546125B2 (en) * 2011-02-21 2013-10-01 Tianjin Greenbio Material Co., Ltd. Recombinant hosts and methods for manufacturing polyhydroxyalkanoate
US20160251609A1 (en) * 2011-10-20 2016-09-01 Scarab Genomics, Llc Reduced Genome Bacteria With Improved Genetic Stability
EP2768943B1 (en) * 2011-10-20 2017-06-21 Scarab Genomics LLC Reduced genome bacteria with improved genetic stability

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003070880A2 (en) 2002-01-23 2003-08-28 Wisconsin Alumni Research Foundation Bacteria with reduced genome
WO2007024756A2 (en) 2005-08-20 2007-03-01 Scarab Genomics, Llc Reduced genome e. coli
JP2011512796A (ja) 2008-02-22 2011-04-28 スカラブ ジェノミクス リミティド ライアビリティ カンパニー アミノ酸生合成のための組成物及び方法
JP2013507113A (ja) 2009-10-08 2013-03-04 グラクソスミスクライン バイオロジカルズ ソシエテ アノニム 発現系

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JP2019058195A (ja) 2019-04-18
EP3113800B1 (en) 2024-06-19
CN106456769A (zh) 2017-02-22
CA2940316A1 (en) 2015-09-11
EP3113800A1 (en) 2017-01-11
EP3113800A4 (en) 2017-10-18
AU2021201686A1 (en) 2021-04-08
US10479820B2 (en) 2019-11-19
WO2015134402A1 (en) 2015-09-11
JP2017508454A (ja) 2017-03-30
KR20160128362A (ko) 2016-11-07
JP6473460B2 (ja) 2019-02-20
AU2015225439A1 (en) 2016-09-01
CN106456769B (zh) 2020-04-17
AU2015225439B2 (en) 2020-12-17
JP6636661B2 (ja) 2020-01-29
CA2940316C (en) 2022-03-15
US20170073379A1 (en) 2017-03-16

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