CN106456769B - 重组crm197在大肠杆菌中的增强产生 - Google Patents

重组crm197在大肠杆菌中的增强产生 Download PDF

Info

Publication number
CN106456769B
CN106456769B CN201580011743.1A CN201580011743A CN106456769B CN 106456769 B CN106456769 B CN 106456769B CN 201580011743 A CN201580011743 A CN 201580011743A CN 106456769 B CN106456769 B CN 106456769B
Authority
CN
China
Prior art keywords
crm197
coli
strain
expression
reduced genome
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201580011743.1A
Other languages
English (en)
Chinese (zh)
Other versions
CN106456769A (zh
Inventor
C.R.布拉特纳
D.A.弗里施
R.E.诺维
T.M.亨克
E.A.斯蒂芬
F.R.布拉特纳
H.崔
G.波斯费
C.F.兰德里
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Scarab Genomics LLC
Original Assignee
Scarab Genomics LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scarab Genomics LLC filed Critical Scarab Genomics LLC
Publication of CN106456769A publication Critical patent/CN106456769A/zh
Application granted granted Critical
Publication of CN106456769B publication Critical patent/CN106456769B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08—Reducing the nucleic acid content
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201580011743.1A 2014-03-03 2015-03-02 重组crm197在大肠杆菌中的增强产生 Active CN106456769B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461947234P 2014-03-03 2014-03-03
US61/947234 2014-03-03
PCT/US2015/018338 WO2015134402A1 (en) 2014-03-03 2015-03-02 Enhanced production of recombinant crm197 in e. coli

Publications (2)

Publication Number Publication Date
CN106456769A CN106456769A (zh) 2017-02-22
CN106456769B true CN106456769B (zh) 2020-04-17

Family

ID=54055763

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201580011743.1A Active CN106456769B (zh) 2014-03-03 2015-03-02 重组crm197在大肠杆菌中的增强产生

Country Status (8)

Country Link
US (1) US10479820B2 (enExample)
EP (1) EP3113800B1 (enExample)
JP (2) JP6473460B2 (enExample)
KR (1) KR102315246B1 (enExample)
CN (1) CN106456769B (enExample)
AU (2) AU2015225439B2 (enExample)
CA (1) CA2940316C (enExample)
WO (1) WO2015134402A1 (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3068887B1 (en) * 2013-11-14 2019-10-30 Scarab Genomics LLC Bacteria with improved metabolic capacity
CA2968491C (en) 2014-11-20 2023-09-26 Biological E Limited Codon optimized polynucleotide for high level expression of crm197
CN107532123A (zh) * 2015-04-02 2018-01-02 斯卡拉布基因组有限责任公司 用于基因组减少的细菌的延长连续流发酵的材料和方法
KR101908590B1 (ko) 2017-02-01 2018-10-16 (주)포바이오코리아 Crm197의 용해성 단백질 발현 및 정제 방법
IL270075B2 (en) 2017-04-22 2024-06-01 Biological E Ltd An improved method for producing a high level of CRM
EP3444269A1 (en) * 2017-08-17 2019-02-20 National Research Council of Canada Systems and methods for the production of diphtheria toxin polypeptides
US20200331973A1 (en) * 2017-09-01 2020-10-22 Lorven Biologics Private Limited Nucleic acid encoding crm197 and process for improved expression thereof
AU2018329671B2 (en) * 2017-09-05 2023-12-07 Scarab Genomics, Llc Methods and compositions for improved expression of recombinant proteins
KR20200111685A (ko) * 2018-01-19 2020-09-29 오비아이 파머 인코퍼레이티드 Crm197 단백질 발현
WO2019178587A1 (en) * 2018-03-16 2019-09-19 Bio-Rad Laboratories, Inc. Purification of target molecules
KR102048456B1 (ko) 2018-03-28 2019-11-25 강원대학교 산학협력단 재조합 대장균을 이용한 rCRM197 생산방법
CN109402035A (zh) * 2018-11-06 2019-03-01 华中农业大学 一种肠外致病性大肠杆菌重组菌株及其应用
KR102099342B1 (ko) * 2019-09-03 2020-04-10 주식회사 제노포커스 Crm197 단백질 발현 방법
NL2023863B1 (en) * 2019-09-20 2021-05-25 Univ Griffith Protein particles and uses thereof
KR102142255B1 (ko) * 2019-12-05 2020-08-07 주식회사 제노포커스 Crm197 단백질 발현 방법
BE1029146B1 (fr) * 2021-02-26 2022-09-26 Xpress Biologics Procede de purification d'une proteine d'interet
BE1029145B1 (fr) * 2021-02-26 2022-09-27 Curavac Europe Methode de production d'une forme periplasmique de la proteine crm197
CN119552230A (zh) * 2024-12-12 2025-03-04 中国人民解放军军事科学院军事医学研究院 一种脂肪酸化修饰的白喉毒素抗原及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1969038A (zh) * 2004-04-26 2007-05-23 味之素株式会社 L－氨基酸生产细菌和生产l－氨基酸的方法
CN102459317A (zh) * 2009-06-25 2012-05-16 大型界面系统校际研究中心 用于crm197及其衍生物的生产的人工基因的细菌表达
CN102639558A (zh) * 2009-10-08 2012-08-15 葛兰素史密丝克莱恩生物有限公司 表达系统

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6989265B2 (en) 2002-01-23 2006-01-24 Wisconsin Alumni Research Foundation Bacteria with reduced genome
US8178339B2 (en) * 2005-08-20 2012-05-15 Scarab Genomics Llc Reduced genome E. coli
JP2009165355A (ja) * 2006-04-28 2009-07-30 Ajinomoto Co Inc Ｌ−アミノ酸を生産する微生物及びｌ−アミノ酸の製造法
JP5506149B2 (ja) * 2007-11-22 2014-05-28 住友化学株式会社 昆虫由来のコリンアセチルトランスフェラーゼ活性に関わる有害生物の生理状態に変化を与える薬剤
JP5778426B2 (ja) 2008-02-22 2015-09-16 スカラブ ジェノミクス リミティド ライアビリティ カンパニー アミノ酸生合成のための組成物及び方法
DE102008063900A1 (de) * 2008-12-19 2010-06-24 Wacker Chemie Ag Verfahren zur fermentativen Herstellung von heterologen Proteinen mittels Escherichia coli
US8546125B2 (en) * 2011-02-21 2013-10-01 Tianjin Greenbio Material Co., Ltd. Recombinant hosts and methods for manufacturing polyhydroxyalkanoate
US20160251609A1 (en) * 2011-10-20 2016-09-01 Scarab Genomics, Llc Reduced Genome Bacteria With Improved Genetic Stability
US9340791B2 (en) * 2011-10-20 2016-05-17 Scarab Genomics, Llc Reduced genome bacteria with improved genetic stability

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1969038A (zh) * 2004-04-26 2007-05-23 味之素株式会社 L－氨基酸生产细菌和生产l－氨基酸的方法
CN102459317A (zh) * 2009-06-25 2012-05-16 大型界面系统校际研究中心 用于crm197及其衍生物的生产的人工基因的细菌表达
CN102639558A (zh) * 2009-10-08 2012-08-15 葛兰素史密丝克莱恩生物有限公司 表达系统

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Furanose-specific Sugar Transport CHARACTERIZATION OF A BACTERIAL GALACTOFURANOSE-BINDING PROTEIN;Richard S. P. Horler等;《THE JOURNAL OF BIOLOGICAL CHEMISTRY》;20091106;第284卷(第45期);第31158页右栏第1段 *
György Pósfai等.REDUCED GENOME Escherichia coli: A PLATFORM FOR GENOMIC AND METABOLIC ENGINEERING.《Conference-EC-US Task Force Joint US-EU Workshop on Metabolomics and Environmental Biotechnology》.2009, *
Overexpression and purfication of the recombinant diphtheria toxin variant CRM197 in Escherichia coli;Alessandra Stefan等;《Journal of Biotechnology》;20110825;第156卷;摘要，第247页 2.4.产物 *

Also Published As

Publication number Publication date
KR20160128362A (ko) 2016-11-07
JP6636661B2 (ja) 2020-01-29
CN106456769A (zh) 2017-02-22
EP3113800A1 (en) 2017-01-11
AU2015225439B2 (en) 2020-12-17
CA2940316C (en) 2022-03-15
EP3113800A4 (en) 2017-10-18
EP3113800B1 (en) 2024-06-19
AU2021201686A1 (en) 2021-04-08
KR102315246B1 (ko) 2021-10-21
CA2940316A1 (en) 2015-09-11
US10479820B2 (en) 2019-11-19
AU2015225439A1 (en) 2016-09-01
WO2015134402A1 (en) 2015-09-11
US20170073379A1 (en) 2017-03-16
JP6473460B2 (ja) 2019-02-20
JP2019058195A (ja) 2019-04-18
JP2017508454A (ja) 2017-03-30

Similar Documents

Publication Publication Date Title
CN106456769B (zh) 重组crm197在大肠杆菌中的增强产生
US12297239B2 (en) E. coli deficient in Lon and SulA for gene targeting
US10287330B2 (en) Methods and compositions relating to CRM197
KR101696986B1 (ko) 변형된 보르데텔라 페르투씨스 균주
JP2017538444A (ja) Ｃｒｍ１９７の高レベル発現のためにコドン最適化したポリヌクレオチド
JP7446227B2 (ja) Ｃｒｍ１９７タンパク質の発現方法
HK1234659A1 (en) Enhanced production of recombinant crm197 in e. coli
HK1234659B (zh) 重组crm197在大肠杆菌中的增强产生
WO2022201917A1 (ja) 大腸菌を用いた外来タンパク質の製造方法
US11365401B2 (en) Oligosaccharyltransferase polypeptide
WO2024146325A1 (zh) 用于生产抗原多糖-载体蛋白生物偶联产物的工程菌及其应用
KR101419214B1 (ko) LacI 단백질 변이체를 이용한 독성 단백질의 생산 방법
HK40009188A (en) Signal polypeptide for improved secretion of protein

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1234659

Country of ref document: HK

GR01 Patent grant
GR01 Patent grant