AU2013206888A1 - Improved DNA polymerase activity assays and methods enabling detection of viable microbes - Google Patents
Improved DNA polymerase activity assays and methods enabling detection of viable microbes Download PDFInfo
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- AU2013206888A1 AU2013206888A1 AU2013206888A AU2013206888A AU2013206888A1 AU 2013206888 A1 AU2013206888 A1 AU 2013206888A1 AU 2013206888 A AU2013206888 A AU 2013206888A AU 2013206888 A AU2013206888 A AU 2013206888A AU 2013206888 A1 AU2013206888 A1 AU 2013206888A1
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- dna polymerase
- assay
- sample matrix
- activity
- pcr
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/101—DNA polymerase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- G01N2333/91205—Phosphotransferases in general
- G01N2333/91245—Nucleotidyltransferases (2.7.7)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
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- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
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- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261583568P | 2012-01-05 | 2012-01-05 | |
| US61/583,568 | 2012-01-05 | ||
| PCT/US2013/020180 WO2013103744A1 (en) | 2012-01-05 | 2013-01-03 | Improved dna polymerase activity assays and methods enabling detection of viable microbes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2013206888A1 true AU2013206888A1 (en) | 2014-08-21 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2013206888A Abandoned AU2013206888A1 (en) | 2012-01-05 | 2013-01-03 | Improved DNA polymerase activity assays and methods enabling detection of viable microbes |
Country Status (10)
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201412316D0 (en) * | 2014-07-10 | 2014-08-27 | Momentum Bioscience Ltd | Detecting the absence of micro-organisms |
| CN107541508A (zh) * | 2016-06-24 | 2018-01-05 | 广州康昕瑞基因健康科技有限公司 | 模板‑引物核酸分子、聚合酶活性测定方法及试剂盒 |
| CN106987643A (zh) * | 2017-05-05 | 2017-07-28 | 广州和实生物技术有限公司 | 一种 Taq DNA 聚合酶活性检测方法 |
| GB201805479D0 (en) | 2018-04-03 | 2018-05-16 | Momentum Bioscience Ltd | Microorganism separation and detection |
| CN109609606B (zh) * | 2018-11-28 | 2023-03-31 | 成都博奥晶芯生物科技有限公司 | 一种dna聚合酶的相对酶活的测定方法 |
| GB201914538D0 (en) | 2019-10-08 | 2019-11-20 | Momentum Bioscience Ltd | Microorganism capture from antimicrobial-containing solution |
| KR102441122B1 (ko) * | 2020-05-11 | 2022-09-07 | 주식회사 엔지노믹스 | 폴리머라제 활성 측정 방법 |
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| US20040005555A1 (en) * | 2000-08-31 | 2004-01-08 | Rothman Richard E. | Molecular diagnosis of bactermia |
| US8883487B2 (en) * | 2004-12-23 | 2014-11-11 | Abbott Point Of Care Inc. | Molecular diagnostics system and methods |
| GB0513535D0 (en) * | 2005-07-01 | 2005-08-10 | Iseao Technologies Ltd | Improved methods of detecting microbes |
| US20080113349A1 (en) * | 2006-11-03 | 2008-05-15 | Pranvera Ikonomi | Method for detecting the presence of mammalian organisms using specific cytochrome c oxidase I (COI) and/or cytochrome b subsequences by a PCR based assay |
| EP2179052B1 (en) * | 2007-07-09 | 2012-11-14 | Microsens Medtech Ltd | Detection of micro-organisms based on their nad-dependent dna ligase activity |
| JP2011188855A (ja) * | 2010-03-11 | 2011-09-29 | Samsung Techwin Co Ltd | 切断可能なキメラプローブを使用したサルモネラのリアルタイム検出方法 |
| AU2011239587B2 (en) | 2010-04-16 | 2016-03-10 | Momentum Bioscience, Ltd. | Methods for measuring enzyme activity useful in determining cell viability in non-purified samples |
| JP2015513921A (ja) * | 2012-04-12 | 2015-05-18 | ゼウス サイエンティフィック、インク． | 任意のポリメラーゼ伸長活性の高感度定量的測定と生細胞存在の決定とに有用なポリメラーゼ活性測定方法 |
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2013
- 2013-01-03 CA CA2897010A patent/CA2897010C/en active Active
- 2013-01-03 AU AU2013206888A patent/AU2013206888A1/en not_active Abandoned
- 2013-01-03 ES ES13733757.2T patent/ES2624871T3/es active Active
- 2013-01-03 US US14/370,752 patent/US9708651B2/en active Active
- 2013-01-03 WO PCT/US2013/020180 patent/WO2013103744A1/en active Application Filing
- 2013-01-03 KR KR1020147021867A patent/KR20150005511A/ko not_active Ceased
- 2013-01-03 EP EP13733757.2A patent/EP2800822B1/en active Active
- 2013-01-03 CN CN201380004900.7A patent/CN104066853B/zh active Active
- 2013-01-03 IN IN6516DEN2014 patent/IN2014DN06516A/en unknown
- 2013-01-03 JP JP2014551321A patent/JP6388540B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2800822A4 (en) | 2015-08-26 |
| CN104066853B (zh) | 2019-05-31 |
| US20150004617A1 (en) | 2015-01-01 |
| JP2015503925A (ja) | 2015-02-05 |
| CN104066853A (zh) | 2014-09-24 |
| JP6388540B2 (ja) | 2018-09-12 |
| EP2800822A1 (en) | 2014-11-12 |
| KR20150005511A (ko) | 2015-01-14 |
| CA2897010A1 (en) | 2013-07-11 |
| ES2624871T3 (es) | 2017-07-17 |
| EP2800822B1 (en) | 2017-03-29 |
| US9708651B2 (en) | 2017-07-18 |
| WO2013103744A1 (en) | 2013-07-11 |
| IN2014DN06516A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 2015-06-12 |
| CA2897010C (en) | 2017-11-07 |
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