IN2014DN06516A - - Google Patents

Info

Publication number
IN2014DN06516A
IN2014DN06516A IN6516DEN2014A IN2014DN06516A IN 2014DN06516 A IN2014DN06516 A IN 2014DN06516A IN 6516DEN2014 A IN6516DEN2014 A IN 6516DEN2014A IN 2014DN06516 A IN2014DN06516 A IN 2014DN06516A
Authority
IN
India
Prior art keywords
dna polymerase
sample matrix
microorganism
amount
selected suitable
Prior art date
Application number
Inventor
Shawn Mark OHARA
Daniel Sweitzig
Original Assignee
Zeus Scientific Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zeus Scientific Inc filed Critical Zeus Scientific Inc
Publication of IN2014DN06516A publication Critical patent/IN2014DN06516A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/91245—Nucleotidyltransferases (2.7.7)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A method for performing a diagnostic assay for the detection of the presence or amount of a microorganism within a sample matrix containing active DNA polymerase is disclosed. The method utilizes the measurement of DNA polymerase extension activity wherein the assay comprises the steps of incubating DNA polymerase in the sample matrix with a selected suitable substrate and performing PCR cycling and detection via the use of a selected suitable nucleic acid probe thereby to detect endogenous DNA polymerase extension activity in the sample matrix as an indication of the presence or amount of said microorganism.
IN6516DEN2014 2012-01-05 2013-01-03 IN2014DN06516A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261583568P 2012-01-05 2012-01-05
PCT/US2013/020180 WO2013103744A1 (en) 2012-01-05 2013-01-03 Improved dna polymerase activity assays and methods enabling detection of viable microbes

Publications (1)

Publication Number Publication Date
IN2014DN06516A true IN2014DN06516A (en) 2015-06-12

Family

ID=48745412

Family Applications (1)

Application Number Title Priority Date Filing Date
IN6516DEN2014 IN2014DN06516A (en) 2012-01-05 2013-01-03

Country Status (10)

Country Link
US (1) US9708651B2 (en)
EP (1) EP2800822B1 (en)
JP (1) JP6388540B2 (en)
KR (1) KR20150005511A (en)
CN (1) CN104066853B (en)
AU (1) AU2013206888A1 (en)
CA (1) CA2897010C (en)
ES (1) ES2624871T3 (en)
IN (1) IN2014DN06516A (en)
WO (1) WO2013103744A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201412316D0 (en) * 2014-07-10 2014-08-27 Momentum Bioscience Ltd Detecting the absence of micro-organisms
CN107541508A (en) * 2016-06-24 2018-01-05 广州康昕瑞基因健康科技有限公司 Templa-primer nucleic acid molecules, polymerase activity assay method and kit
CN106987643A (en) * 2017-05-05 2017-07-28 广州和实生物技术有限公司 A kind of Taq DNA polymerase activity detection methods
GB201805479D0 (en) 2018-04-03 2018-05-16 Momentum Bioscience Ltd Microorganism separation and detection
CN109609606B (en) * 2018-11-28 2023-03-31 成都博奥晶芯生物科技有限公司 Method for determining relative enzyme activity of DNA polymerase
GB201914538D0 (en) 2019-10-08 2019-11-20 Momentum Bioscience Ltd Microorganism capture from antimicrobial-containing solution
KR102441122B1 (en) * 2020-05-11 2022-09-07 주식회사 엔지노믹스 Method for measuring polymerase activity

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040005555A1 (en) * 2000-08-31 2004-01-08 Rothman Richard E. Molecular diagnosis of bactermia
JP5165383B2 (en) * 2004-12-23 2013-03-21 アイ−スタツト・コーポレイシヨン Molecular diagnostic system and method
GB0513535D0 (en) * 2005-07-01 2005-08-10 Iseao Technologies Ltd Improved methods of detecting microbes
US20080113349A1 (en) * 2006-11-03 2008-05-15 Pranvera Ikonomi Method for detecting the presence of mammalian organisms using specific cytochrome c oxidase I (COI) and/or cytochrome b subsequences by a PCR based assay
MX2010000031A (en) * 2007-07-09 2010-04-21 Microsens Medtech Ltd Detection of micro-organisms based on their nad-dependent dna ligase activity.
JP2011188855A (en) * 2010-03-11 2011-09-29 Samsung Techwin Co Ltd Method for real-time detection of salmonella using cleavable chimeric probe
EP2558600B2 (en) * 2010-04-16 2020-06-17 Momentum Bioscience Limited Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
AU2013245832A1 (en) * 2012-04-12 2014-11-27 Zeus Scientific, Inc. Methods for measuring polymerase activity useful for sensitive, quantitative measurements of any polymerase extension activity and for determining the presence of viable cells

Also Published As

Publication number Publication date
JP2015503925A (en) 2015-02-05
EP2800822A1 (en) 2014-11-12
WO2013103744A1 (en) 2013-07-11
US20150004617A1 (en) 2015-01-01
EP2800822B1 (en) 2017-03-29
US9708651B2 (en) 2017-07-18
JP6388540B2 (en) 2018-09-12
CN104066853B (en) 2019-05-31
CA2897010A1 (en) 2013-07-11
CN104066853A (en) 2014-09-24
EP2800822A4 (en) 2015-08-26
KR20150005511A (en) 2015-01-14
AU2013206888A1 (en) 2014-08-21
CA2897010C (en) 2017-11-07
ES2624871T3 (en) 2017-07-17

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