AU2012222502A1 - Liquid phase separation of plasmid DNA isoforms and topoisomers - Google Patents

Liquid phase separation of plasmid DNA isoforms and topoisomers Download PDF

Info

Publication number
AU2012222502A1
AU2012222502A1 AU2012222502A AU2012222502A AU2012222502A1 AU 2012222502 A1 AU2012222502 A1 AU 2012222502A1 AU 2012222502 A AU2012222502 A AU 2012222502A AU 2012222502 A AU2012222502 A AU 2012222502A AU 2012222502 A1 AU2012222502 A1 AU 2012222502A1
Authority
AU
Australia
Prior art keywords
group
branched
unbranched
use according
ligand
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2012222502A
Other languages
English (en)
Inventor
Michael Lammerhofer
Wolfgang Lindner
Marek Mahut
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim RCV GmbH and Co KG
Original Assignee
Boehringer Ingelheim RCV GmbH and Co KG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim RCV GmbH and Co KG filed Critical Boehringer Ingelheim RCV GmbH and Co KG
Publication of AU2012222502A1 publication Critical patent/AU2012222502A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3257—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one of the heteroatoms nitrogen, oxygen or sulfur together with at least one silicon atom, these atoms not being part of the carrier as such
    • B01J20/3263—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one of the heteroatoms nitrogen, oxygen or sulfur together with at least one silicon atom, these atoms not being part of the carrier as such comprising a cyclic structure containing at least one of the heteroatoms nitrogen, oxygen or sulfur, e.g. an heterocyclic or heteroaromatic structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/80—Aspects related to sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J2220/82—Shaped bodies, e.g. monoliths, plugs, tubes, continuous beds

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
AU2012222502A 2011-02-28 2012-02-27 Liquid phase separation of plasmid DNA isoforms and topoisomers Abandoned AU2012222502A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP11156235.1 2011-02-28
EP11156235 2011-02-28
PCT/EP2012/053241 WO2012116945A2 (en) 2011-02-28 2012-02-27 Liquid phase separation of plasmid dna isoforms and topoisomers

Publications (1)

Publication Number Publication Date
AU2012222502A1 true AU2012222502A1 (en) 2013-08-01

Family

ID=44168483

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2012222502A Abandoned AU2012222502A1 (en) 2011-02-28 2012-02-27 Liquid phase separation of plasmid DNA isoforms and topoisomers

Country Status (9)

Country Link
US (1) US20120220729A1 (ja)
EP (1) EP2680932A2 (ja)
JP (1) JP2014512805A (ja)
KR (1) KR20140033006A (ja)
CN (1) CN103501871A (ja)
AU (1) AU2012222502A1 (ja)
CA (1) CA2825248A1 (ja)
SG (1) SG192983A1 (ja)
WO (1) WO2012116945A2 (ja)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201113698D0 (en) * 2011-08-09 2011-09-21 Wirtz Ralf M Matrix and method for purifying and/or isolating nucleic acids
WO2016197491A1 (en) * 2015-06-12 2016-12-15 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences A three-dimensional tissue scaffold with stem cell attracting element and use thereof
CN107688055A (zh) * 2016-08-03 2018-02-13 湖北生物医药产业技术研究院有限公司 用于核酸药物线性dna含量的检测方法
CN113440598B (zh) * 2020-03-24 2023-08-01 深圳翰宇药业股份有限公司 一种拓扑杂质的稳定方法
CN111721871A (zh) * 2020-06-24 2020-09-29 南京济群生物科技有限公司 一种质粒超螺旋dna含量的高分离度检测方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3550397B2 (ja) 1991-05-13 2004-08-04 マサチユセツツ・インスチチユート・オブ・テクノロジイ 複素環式キラル配位子およびオレフィン類の接触非対称性ジヒドロキシル化方法
DE69727679T2 (de) 1996-06-05 2004-07-22 Lindner, Wolfgang, Prof. Dr. Auf cinchonan basierte chirale selektoren zur trennung von stereoisomeren
US6197994B1 (en) 1998-03-26 2001-03-06 Korea Institute Of Science And Technology Silica gel supported bis-cinchona alkaloid derivatives and a preparation method and use thereof
MXPA01012213A (es) 1999-05-28 2003-06-24 Bio Science Contract Productio Metodos de purificacion de adn y adn purificado.
US6616825B1 (en) 2000-08-23 2003-09-09 The Regents Of The University Of California Electrochromatographic device for use in enantioselective separation, and enantioselective separation medium for use therein
PL375533A1 (en) * 2002-08-14 2005-11-28 Neurosearch A/S Novel quinuclidine derivatives and their use
JP4291628B2 (ja) * 2003-06-12 2009-07-08 株式会社資生堂 液体クロマトグラフ装置及び試料に含まれる光学異性体の分析方法
EP1693108A1 (en) * 2004-12-04 2006-08-23 MERCK PATENT GmbH Mixed-modal anion-exchange type separation material

Also Published As

Publication number Publication date
KR20140033006A (ko) 2014-03-17
CA2825248A1 (en) 2012-09-07
CN103501871A (zh) 2014-01-08
WO2012116945A2 (en) 2012-09-07
SG192983A1 (en) 2013-09-30
US20120220729A1 (en) 2012-08-30
JP2014512805A (ja) 2014-05-29
WO2012116945A3 (en) 2012-12-13
EP2680932A2 (en) 2014-01-08

Similar Documents

Publication Publication Date Title
US11845772B2 (en) Ribonucleic acid purification
JP2011518337A (ja) クロマトグラフィー媒体
US20040002081A1 (en) Method and device for isolating and purifying a polynucleotide of interest on a manufacturing scale
AU2012222502A1 (en) Liquid phase separation of plasmid DNA isoforms and topoisomers
RO122855B1 (ro) Metode de purificare a adn-ului şi adn-uri purificate
US8093373B2 (en) Plasmid purification
KR20210134639A (ko) 음이온 교환 크로마토그래피를 이용한 플라스미드 dna와 같은 생물학적 분자의 정제 공정
JP2024527602A (ja) アデノ随伴ウイルスカプシドを分離するための方法、前記方法によって得られる組成物及びその使用
JP2006512457A (ja) 収着剤、及びプラスミドｄｎａの分離方法
EP1321176A1 (en) Method and device for isolating and purifying a polynucleotide of interest on a manufacturing scale
WO2016130928A1 (en) Aminoglycoside hydrogel microbeads and macroporous gels with chemical crosslink, method of preparation and use thereof
Caramelo-Nunes et al. Aromatic ligands for plasmid deoxyribonucleic acid chromatographic analysis and purification: An overview
JP2003514655A (ja) 核酸構造を有する化合物を含むサンプル由来の物質の選択的除去方法
Yu et al. RECENT DEVELOPMENT AND APPLICATION OF MONOLITHIC COLUMNS.
KR101569832B1 (ko) 시료 중의 게놈 ｄｎａ 및 플라스미드 ｄｎａ를 서로 분리하는 방법 및 그를 위한 키트
JP2005517445A (ja) 芳香族チオエーテルリガンドを用いた分離法
WO2017115655A1 (ja) 分離分析方法
US20090047734A1 (en) Method of separation of deoxyribonucleic acids
EP1455920B1 (en) Separation method
ES2263038T3 (es) Aislamiento de oligonucleotidos antisentido.
US9610576B2 (en) Hydrolytically stable ion-exchange stationary phases and uses thereof
WO2017115652A1 (ja) 分離分析方法
TW202346312A (zh) 於核酸純化中降低內毒素含量之方法
Jonsson Investigation of pore size effects at separation of oligonucleotides using Ion-pair RP HPLC: Examining of how the particle pore size of the stationary phase affects separations of oligonucleotides in therapeutic range
WO2024141249A1 (en) Purification of biological particles

Legal Events

Date Code Title Description
MK1 Application lapsed section 142(2)(a) - no request for examination in relevant period