AU2010207880B2 - Measuring G protein coupled receptor activation - Google Patents

Measuring G protein coupled receptor activation Download PDF

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Publication number
AU2010207880B2
AU2010207880B2 AU2010207880A AU2010207880A AU2010207880B2 AU 2010207880 B2 AU2010207880 B2 AU 2010207880B2 AU 2010207880 A AU2010207880 A AU 2010207880A AU 2010207880 A AU2010207880 A AU 2010207880A AU 2010207880 B2 AU2010207880 B2 AU 2010207880B2
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AU
Australia
Prior art keywords
protein
cell
receptor
subunit
coupled receptor
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AU2010207880A
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AU2010207880A1 (en
Inventor
Helen Dacres
Irene Mary Horne
Virginia Leitch
Stephen Charles Trowell
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Commonwealth Scientific and Industrial Research Organization CSIRO
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Commonwealth Scientific and Industrial Research Organization CSIRO
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Mycology (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AU2010207880A 2009-01-29 2010-01-28 Measuring G protein coupled receptor activation Ceased AU2010207880B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14827109P 2009-01-29 2009-01-29
US61/148,271 2009-01-29
PCT/AU2010/000080 WO2010085844A1 (en) 2009-01-29 2010-01-28 Measuring g protein coupled receptor activation

Publications (2)

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AU2010207880A1 AU2010207880A1 (en) 2010-08-05
AU2010207880B2 true AU2010207880B2 (en) 2012-03-29

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AU2010207880A Ceased AU2010207880B2 (en) 2009-01-29 2010-01-28 Measuring G protein coupled receptor activation

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US (1) US8647887B2 (enExample)
EP (1) EP2382470B1 (enExample)
JP (1) JP5684145B2 (enExample)
KR (1) KR101834574B1 (enExample)
CN (1) CN102439444B (enExample)
AU (1) AU2010207880B2 (enExample)
CA (1) CA2748500C (enExample)
IL (1) IL213771A0 (enExample)
NZ (1) NZ593294A (enExample)
SG (1) SG172321A1 (enExample)
WO (1) WO2010085844A1 (enExample)

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CN107074926B (zh) * 2014-10-14 2021-09-21 蒙特利尔大学 基于Gβγ互作蛋白监测G蛋白激活的生物传感器
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CN108586620B (zh) * 2018-05-10 2021-11-23 山东大学 筛选昆虫性信息素受体的配体的传感器分子以及筛选方法
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US11078200B2 (en) 2018-06-29 2021-08-03 International Paper Company Synthesis of coelenterazine
CN110252079B (zh) * 2019-05-08 2022-03-15 天津大学 一种气味远程传输的方法
KR102264116B1 (ko) * 2019-06-19 2021-06-14 한국과학기술연구원 세포 소포체 내 g-단백질 연결 수용체(gpcr)의 실시간 활성 상태 분석 방법 및 컴퓨터 프로그램
CN110577593B (zh) * 2019-07-01 2021-09-14 华中农业大学 一种小分子近红外光荧光蛋白及其融合蛋白
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DE102023204533B4 (de) * 2023-05-15 2025-03-27 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung eingetragener Verein Verfahren für die Durchführung von biochemischen Untersuchungen an Proben
CN118501118B (zh) * 2024-07-16 2024-10-29 汉王科技股份有限公司 一种基于细胞的生物荧光能量共振转移检测系统及应用
CN119409840B (zh) * 2025-01-06 2025-07-01 北京大学 基于g蛋白偶联受体构建的比率型荧光探针及其构建方法

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WO2004057333A1 (en) * 2002-12-20 2004-07-08 Julius-Maximilians-Uni Versität Würzburg Millisecond activation switch for seven-transmembrane proteins
WO2005105850A1 (en) * 2004-05-04 2005-11-10 Universite De Montreal Double brilliance beta-arrestin: a biosensor for monitoring the activity of receptors and signalling molecules, and method of using same
WO2006086883A1 (en) * 2005-02-16 2006-08-24 Universite De Montreal Biosensors for monitoring receptor-mediated g-protein activation

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US20120077210A1 (en) 2012-03-29
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CA2748500A1 (en) 2010-08-05
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