AU2008224877B2 - Virus like particle purification - Google Patents

Virus like particle purification Download PDF

Info

Publication number
AU2008224877B2
AU2008224877B2 AU2008224877A AU2008224877A AU2008224877B2 AU 2008224877 B2 AU2008224877 B2 AU 2008224877B2 AU 2008224877 A AU2008224877 A AU 2008224877A AU 2008224877 A AU2008224877 A AU 2008224877A AU 2008224877 B2 AU2008224877 B2 AU 2008224877B2
Authority
AU
Australia
Prior art keywords
vlps
chromatographic
chromatography
vlp
column
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2008224877A
Other languages
English (en)
Other versions
AU2008224877A1 (en
Inventor
Thomas R. Foubert
Charles R. Petrie
Charles Richardson
Bryan Steadman
Thomas S. Vedvick
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Takeda Vaccines Montana Inc
Original Assignee
Ligocyte Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ligocyte Pharmaceuticals Inc filed Critical Ligocyte Pharmaceuticals Inc
Publication of AU2008224877A1 publication Critical patent/AU2008224877A1/en
Application granted granted Critical
Publication of AU2008224877B2 publication Critical patent/AU2008224877B2/en
Priority to AU2013242822A priority Critical patent/AU2013242822B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12Antivirals
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/12Viral antigens
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00Medicinal preparations characterised by special physical form
    • A61K9/14Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00Medicinal preparations characterised by special physical form
    • A61K9/20Pills, tablets, discs, rods
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00Drugs for immunological or allergic disorders
    • A61P37/02Immunomodulators
    • A61P37/04Immunostimulants
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/18Ion-exchange chromatography
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02Recovery or purification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/16011Caliciviridae
    • C12N2770/16023Virus like particles [VLP]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/16011Caliciviridae
    • C12N2770/16034Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/16011Caliciviridae
    • C12N2770/16051Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2008224877A 2007-03-14 2008-03-14 Virus like particle purification Active AU2008224877B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2013242822A AU2013242822B2 (en) 2007-03-14 2013-10-10 Virus like particle purification

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US90682107P 2007-03-14 2007-03-14
US60/906,821 2007-03-14
PCT/US2008/057072 WO2008113011A2 (en) 2007-03-14 2008-03-14 Virus like particle purification

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2013242822A Division AU2013242822B2 (en) 2007-03-14 2013-10-10 Virus like particle purification

Publications (2)

Publication Number Publication Date
AU2008224877A1 AU2008224877A1 (en) 2008-09-18
AU2008224877B2 true AU2008224877B2 (en) 2013-07-11

Family

ID=39760420

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2008224877A Active AU2008224877B2 (en) 2007-03-14 2008-03-14 Virus like particle purification

Country Status (12)

Country Link
US (4) US8481693B2 (enExample)
EP (1) EP2134360B1 (enExample)
JP (4) JP5284290B2 (enExample)
KR (3) KR20170001720A (enExample)
AU (1) AU2008224877B2 (enExample)
CA (1) CA2683977C (enExample)
DK (1) DK2134360T3 (enExample)
ES (1) ES2559421T3 (enExample)
HU (1) HUE028605T2 (enExample)
PL (1) PL2134360T3 (enExample)
SG (2) SG179488A1 (enExample)
WO (1) WO2008113011A2 (enExample)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUE032422T2 (en) 2006-09-29 2017-09-28 Takeda Vaccines Inc Norovirus vaccine preparations
CA2683977C (en) * 2007-03-14 2017-04-25 Ligocyte Pharmaceuticals, Inc. A method of norovirus virus-like particle purification comprising ion exchange chromatography
SG10201601660YA (en) 2007-09-18 2016-04-28 Takeda Vaccines Inc Method of conferring a protective immune response to norovirus
US20100266636A1 (en) 2007-09-18 2010-10-21 Ligocyte Pharmaceuticals, Inc. Method of conferring a protective immune response to norovirus
HUE040636T2 (hu) 2009-06-16 2019-03-28 Genzyme Corp Javított eljárások rekombináns AAV-vektorok tisztítására
US20120164710A1 (en) * 2009-07-03 2012-06-28 Yuan Yuan Fan Method of preparation of a biological particulate structure
US20110142863A1 (en) 2009-12-16 2011-06-16 Millipore Corporation Flow through purification processes for large biomolecules
WO2011088225A1 (en) 2010-01-15 2011-07-21 Bio-Rad Laboratories, Inc. Surface neutralization of apatite
JP2013523175A (ja) * 2010-04-14 2013-06-17 イーエムディー・ミリポア・コーポレーション 高力価、高純度のウイルスストックの作製方法及びその使用方法
DE102010046817A1 (de) * 2010-09-28 2012-03-29 Sartorius Stedim Biotech Gmbh Verfahren zur Abtrennung von Viren aus einem Kontaminanten enthaltenden flüssigen Medium
FI122520B (fi) * 2010-10-15 2012-03-15 Vesna Blazevic Noroviruksen kapsidi ja rotaviruksen VP6-proteiini käytettäväksi yhdistelmärokotteena
EP2670704B1 (en) 2011-02-02 2018-12-12 Bio-Rad Laboratories, Inc. Apatite surface neutralization with alkali solutions
EA029470B1 (ru) 2011-07-11 2018-03-30 Такеда Вэксинс, Инк. Способ стимулирования формирования защитного иммунитета против норовируса
EP2554664A1 (de) * 2011-08-02 2013-02-06 Life Science Inkubator Verfahren zur Aufreinigung von Virus-ähnlichen Partikeln (VLP)
CN104023745B (zh) * 2011-12-30 2016-12-07 德国癌症研究中心 用于接种目的的来自eb病毒的第二代病毒样颗粒(vlp)
US9815695B2 (en) 2012-05-30 2017-11-14 Bio-Rad Laboratories, Inc. In situ restoration of apatite-based chromatography resins
JOP20130186B1 (ar) * 2012-06-22 2021-08-17 Takeda Vaccines Montana Inc تنقية الجزيئات الشبيهة بالفيروسات
US9902942B2 (en) 2013-02-22 2018-02-27 Agency For Science, Technology And Research Chromatographic purification of virus preparations with negatively charged particles
JP6479305B2 (ja) 2013-07-12 2019-03-06 株式会社Umnファーマ ウイルス様粒子の精製方法
JP2015015931A (ja) * 2013-07-12 2015-01-29 株式会社Umnファーマ ウイルス様粒子を含む培養物の製造方法
JP2015017065A (ja) * 2013-07-12 2015-01-29 株式会社Umnファーマ ノロウイルスのウイルス様粒子を含む医薬組成物
SG10202107054TA (en) 2013-10-03 2021-08-30 Takeda Vaccines Inc Methods of detection and removal of rhabdoviruses from cell lines
US9802822B2 (en) 2014-06-23 2017-10-31 Bio-Rad Laboratories, Inc. Apatite pretreatment
WO2015200299A2 (en) 2014-06-23 2015-12-30 Bio-Rad Laboratories, Inc. Apatite in-situ restoration
CN107603959A (zh) * 2017-08-30 2018-01-19 四川大学 提高缓冲液盐离子浓度纯化病毒的方法
IL316292A (en) * 2017-12-07 2024-12-01 Emp Biotech Gmbh System and methods for applied radial chromatography technology
JP6704074B2 (ja) * 2019-02-04 2020-06-03 株式会社Umnファーマ ウイルス様粒子の精製方法
CN114008067B (zh) * 2019-07-04 2025-08-05 株式会社钟化 病毒或病毒样颗粒的纯化方法
WO2022044727A1 (ja) * 2020-08-28 2022-03-03 株式会社カネカ 有用物質の精製方法
EP4225462A1 (en) * 2020-10-05 2023-08-16 Sartorius BIA Separations d.o.o. Improved removal of rna and contaminants from dna plasmid preparations by hydrophobic interaction chromatography
IL304890A (en) * 2021-02-02 2023-10-01 Nichimo Corp A solar array generating system and a reflector for a solar array generating system
US20240238401A1 (en) 2021-05-21 2024-07-18 Takeda Vaccines, Inc. Solid composition, freeze-drying method and glass vial
WO2025077800A1 (en) * 2023-10-12 2025-04-17 Chengdu Kanghua Biological Products Co., Ltd. Purification of norovirus virus-like particles (vlps)
WO2025077807A1 (en) * 2023-10-12 2025-04-17 Chengdu Kanghua Biological Products Co., Ltd. Methods for obtaining high-purity norovirus virus-like particles (vlps)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7041500B2 (en) * 2002-02-14 2006-05-09 Novavax, Inc. Insect cell line
WO2006136566A1 (en) * 2005-06-21 2006-12-28 Cytos Biotechnology Ag Process for the preparative purification of virus-like particles (vlps)

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2833106B2 (ja) 1990-02-21 1998-12-09 株式会社島津製作所 液体クロマトグラフ
WO1994026087A2 (en) 1993-05-14 1994-11-24 Connor Kim C O Recombinant protein production and insect cell culture and process
BR9507657A (pt) * 1994-05-16 1997-09-09 Merck & Co Inc Proteina isolada e purificada de papilomavirus recombinante capsideos particulas semelhantes a virus processos a virus processos para produzir a proteina para tratar ou prevenir a infecçao por papilomavirus para produzir uma proteina de capsideo de papilomavirus recombinante montada em um capsideo ou particula semelhante a virus e para induzir uma resposta imune em um animal vertebrado composiçoes farmacêuticas kit para a detecçao de papilomavirus ou anticorpos para papilomavirus e sistema sinte
AR004464A1 (es) * 1994-11-14 1998-12-16 Merck Sharp & Dohme Un metodo para producir una proteina de capside de papilomavirus
JP3316325B2 (ja) 1994-12-20 2002-08-19 富士シリシア化学株式会社 ビール安定化処理用シリカゲル及びその製造方法並びにビールの安定化処理方法
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
AU738585B2 (en) 1996-11-08 2001-09-20 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Synthesis and purification of hepatitis C virus-like particles
ES2268787T3 (es) * 1997-09-05 2007-03-16 Medimmune, Inc. Metodo in vitro de desmontaje/embalaje de particulas similares a virus (vlp) de papilomavirus.
DE19823814A1 (de) 1998-05-27 1999-12-02 Octapharma Ag Ziegelbruecke Verfahren zur Trennung und/oder Isolierung von Plasmaproteinen mit annularer Chromatographie
BR9913026A (pt) 1998-08-14 2001-09-25 Merck & Co Inc Processo de purificação de partìculas semelhantes a vìrus (vlps) de papilomavìrus recombinante, e, processo de preparação de um produto de vlp de papilomavìrus humano purificado adequado para uso em uma vacina para humano
US6349843B1 (en) 1999-04-01 2002-02-26 The Vollrath Company Llc Pan removal ramp
KR100771402B1 (ko) * 1999-06-22 2007-10-30 국립감염증연구소장이 대표하는 일본국 Srsv 검출 킷트
DE10012732A1 (de) * 2000-03-18 2001-09-20 Aventis Behring Gmbh Thrombin-Zubereitungen und Verfahren zu ihrer Herstellung
JP2003066021A (ja) 2001-08-28 2003-03-05 Hiroshi Shionoya 連続分取液体クロマトグラフィー装置とそれを用いる方法
AU2003265235A1 (en) 2002-04-26 2003-12-19 Genetech, Inc. Non-affinity purification of proteins
CA2496918A1 (en) 2002-08-28 2004-03-11 Introgen Therapeutics Inc. Chromatographic methods for adenovirus purification
WO2005032457A2 (en) * 2003-07-21 2005-04-14 Boyce Thompson Institute For Plant Research, Inc. Vectors and methods for immunization against norwalk virus using transgenic plants
US7481997B1 (en) * 2004-02-12 2009-01-27 Montana State University Snow mountain virus genome sequence, virus-like particles and methods of use
US8277819B2 (en) * 2005-06-16 2012-10-02 Children's Hospital Medical Center Norovirus particle for use as an antiviral or vaccine
JP5215865B2 (ja) * 2005-11-22 2013-06-19 ノバルティス ヴァクシンズ アンド ダイアグノスティクス インコーポレイテッド ノロウイルス抗原およびサポウイルス抗原
US20080226669A1 (en) 2005-12-29 2008-09-18 Boehringer Ingelheim Vetmedica, Inc. Multivalent pcv2 immunogenic compositions and methods of producing such compositions
EP2044198A4 (en) 2006-07-27 2010-09-08 Ligocyte Pharmaceuticals Inc CHIMERIC INFLUENZA VIRUS TYPE PARTICLES
HUE032422T2 (en) * 2006-09-29 2017-09-28 Takeda Vaccines Inc Norovirus vaccine preparations
US8202967B2 (en) 2006-10-27 2012-06-19 Boehringer Ingelheim Vetmedica, Inc. H5 proteins, nucleic acid molecules and vectors encoding for those, and their medicinal use
CA2683977C (en) 2007-03-14 2017-04-25 Ligocyte Pharmaceuticals, Inc. A method of norovirus virus-like particle purification comprising ion exchange chromatography
EP2027875A1 (en) 2007-08-23 2009-02-25 Octapharma AG A Process for Isolation and Purification of a Target Protein free of Prion Protein (PrPSC)
SG10201601660YA (en) 2007-09-18 2016-04-28 Takeda Vaccines Inc Method of conferring a protective immune response to norovirus
WO2009080781A1 (en) 2007-12-21 2009-07-02 Cytos Biotechnology Ag Process for clarifying cell homogenates
AU2009279456B2 (en) * 2008-08-08 2015-02-05 Takeda Vaccines, Inc. Virus-like particles comprising composite capsid amino acid sequences for enhanced cross reactivity
US20110262483A1 (en) 2008-11-03 2011-10-27 Ligocyte Pharmaceuticals, Inc. Methods for isolating enveloped virus-based vlps free of infectious agents
US8945895B2 (en) 2009-07-31 2015-02-03 Baxter International Inc. Methods of purifying recombinant ADAMTS13 and other proteins and compositions thereof
SG182636A1 (en) * 2010-01-21 2012-08-30 Ligocyte Pharmaceuticals Inc Targeted heterologous antigen presentation on calicivirus virus-like particles
CA2812058C (en) 2010-09-20 2019-04-16 Bio-Rad Laboratories, Inc. Dissociation of product-complexed contaminants in chromatography
FI122520B (fi) 2010-10-15 2012-03-15 Vesna Blazevic Noroviruksen kapsidi ja rotaviruksen VP6-proteiini käytettäväksi yhdistelmärokotteena
CN102260651A (zh) 2011-06-24 2011-11-30 华中科技大学同济医学院附属协和医院 一种纯化病毒样颗粒蛋白的方法及其应用
JOP20130186B1 (ar) 2012-06-22 2021-08-17 Takeda Vaccines Montana Inc تنقية الجزيئات الشبيهة بالفيروسات
JP2015015931A (ja) 2013-07-12 2015-01-29 株式会社Umnファーマ ウイルス様粒子を含む培養物の製造方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7041500B2 (en) * 2002-02-14 2006-05-09 Novavax, Inc. Insect cell line
WO2006136566A1 (en) * 2005-06-21 2006-12-28 Cytos Biotechnology Ag Process for the preparative purification of virus-like particles (vlps)

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NICOLLIER-JAMOT et al, VACCINE (2004), VOL 22, PAGES 1079-1086 *

Also Published As

Publication number Publication date
ES2559421T3 (es) 2016-02-12
DK2134360T3 (en) 2016-02-22
US10172930B2 (en) 2019-01-08
WO2008113011A2 (en) 2008-09-18
US20190282686A1 (en) 2019-09-19
SG179488A1 (en) 2012-04-27
US20100150961A1 (en) 2010-06-17
US8481693B2 (en) 2013-07-09
HUE028605T2 (en) 2016-12-28
US20130344107A1 (en) 2013-12-26
AU2008224877A1 (en) 2008-09-18
WO2008113011A3 (en) 2008-12-24
US20160317645A1 (en) 2016-11-03
JP5284290B2 (ja) 2013-09-11
JP2019068846A (ja) 2019-05-09
US9359410B2 (en) 2016-06-07
EP2134360A4 (en) 2011-08-24
EP2134360A2 (en) 2009-12-23
CA2683977C (en) 2017-04-25
KR101576219B1 (ko) 2015-12-10
US10792353B2 (en) 2020-10-06
PL2134360T3 (pl) 2016-05-31
EP2134360B1 (en) 2015-11-18
SG10201701988TA (en) 2017-04-27
JP2017086090A (ja) 2017-05-25
CA2683977A1 (en) 2008-09-18
HK1138795A1 (zh) 2010-09-03
JP2010530734A (ja) 2010-09-16
KR20170001720A (ko) 2017-01-04
KR20150098681A (ko) 2015-08-28
JP2013176392A (ja) 2013-09-09
KR20100015562A (ko) 2010-02-12

Similar Documents

Publication Publication Date Title
US10792353B2 (en) Virus like particle purification
Li et al. A hydrophobic interaction chromatography strategy for purification of inactivated foot-and-mouth disease virus
EP2361975B1 (en) Method for influenza virus purification
CN107567496A (zh) 有关病毒的无菌纯化方法
US20250034593A1 (en) Method for Adenovirus Purification
Zhao et al. Enterovirus71 virus‐like particles produced from insect cells and purified by multistep chromatography elicit strong humoral immune responses in mice
JP7586936B2 (ja) 混入dnaをより効果的に除去するための、アデノ随伴ウイルスの精製の強化
AU2013242822B2 (en) Virus like particle purification
AU2016269506A1 (en) Virus like particle purification
HK1138795B (en) Virus like particle purification
CN107603959A (zh) 提高缓冲液盐离子浓度纯化病毒的方法

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)