JP2023529187A - 混入dnaをより効果的に除去するための、アデノ随伴ウイルスの精製の強化 - Google Patents
混入dnaをより効果的に除去するための、アデノ随伴ウイルスの精製の強化 Download PDFInfo
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Abstract
Description
(a)表面に正電荷を担持する固相を用いてDNAの抽出を行って、第1の画分を得ることであって、ここで、上記固相は、pH7.0±1.0、塩濃度10mM~200mMで調製物と接触させられ;
(b)上記第1の画分を、第1のタンジェンシャルフローフィルトレーション(tangential flow filtration)によりダイアフィルトレーションして(diafiltering)第2の画分を得ること;
(c)上記第2の画分をDNAseで処理すること;
(d)ステップ(c)で得られた、DNAse処理された第2の画分を、pH7.0±1.0及び塩濃度10mM~20mMを有する緩衝液に、第2のタンジェンシャルフローフィルトレーションによりダイアフィルトレーションして第3の画分を得ること;及び
任意に(optionally)、
(e)補足的なクロマトグラフィの前に、第3の画分をタンジェンシャルフローフィルトレーションにより濃縮すること。
オプション(i)陽イオン交換クロマトグラフィ、
オプション(ii)アフィニティークロマトグラフィ、又は、
オプション(iii)陰イオン交換クロマトグラフィ。
陽イオン交換クロマトグラフィ材料を約pH3.5±0.5へ再平衡化すること、
塩勾配を用いた溶出を行うこと、及び
その後、陰イオン交換クロマトグラフィ処理を行うこと、
が行われる。このプロセスにより、例えば、臨床品質のAAVが得られる。
最初に、AAVセロタイプ8を含む細胞ライセート300mLに、5mLの正電荷粒子を添加した。この粒子は、表面にエチレンジアミンが固定化された、サイズ範囲が20μm~40μmであり、平均孔径が2μmの非球面ポリメタクリレート粒子であった。固相表面に固定化されたエチレンジアミンは、各残基が1級アミン及び2級アミンを有することにより、正に帯電している。この調製物を30分間攪拌した。10,000×gで10分間遠心分離して、0.45μmPES膜でろ過することにより、粒子を除去した。
実施例1に記載されたものと同じ材料の試料を、第2のTFFステップを通して同様に処理した。次に、2M ギ酸を徐々に添加することにより、pH3.5に滴定した。
アフィニティークロマトグラフィを用いた実験対照を実行して、他のAAV捕捉方法に対する本発明の方法を用いることの潜在的利点を実証した。AAVアフィニティーカラムを50mM リン酸塩、100mM NaCl、pH7.2で平衡化した。試料を、同じpHに平行化し、ろ過した後、カラムにロードした。カラムを平衡化緩衝液で洗浄した後、50mM グリシン、pH3.0へのステップで溶出させた。溶出後、カラムを25mM 水酸化ナトリウムで洗浄した。
第2のTFFステップまでの本発明のステップは行うが、その後のpHを下げるステップ及び陽イオン交換体に試料を付与するステップは行わない。その代わりに、試料をアフィニティークロマトグラフィカラムに直接付与する。その後、カラムを洗浄し、本発明の強化を用いない場合と同様に溶出する。
第2のTFFステップまでの本発明のステップは行うが、その後のpHを下げるステップ及び陽イオン交換体に試料を付与するステップは行わない。その代わりに、試料を、AAVが同じ条件に平衡化された陰イオン交換体に結合するように、十分に低い伝導度(塩濃度)に希釈される。その後、カラムを洗浄し、本発明の強化を用いない場合と同様に溶出する。
上述の実施例のいずれも、正に帯電した粒子を、大きな正に帯電した表面に置換することによって実施することができる。このような表面は、デプスフィルタなどの、多くの市販のろ過媒体に見られる。
Claims (10)
- 以下のステップを含む、AAVカプシド及び混入DNAを含む調製物の混入DNA含量を低減する方法:
(a)表面に正電荷を担持する固相を用いてDNAの抽出を行って、第1の画分を得ることであって、ここで、前記固相は、pH7.0±1.0、塩濃度10mM~200mMで調製物と接触させられ;
(b)前記第1の画分を、第1のタンジェンシャルフローフィルトレーション(tangential flow filtration)によりダイアフィルトレーションして(diafiltering)第2の画分を得ること;
(c)前記第2の画分をDNAseで処理すること;
(d)ステップ(c)で得られた、DNAse処理された第2の画分を、pH7.0±1.0及び塩濃度10mM~20mMを有する緩衝液に、第2のタンジェンシャルフローフィルトレーションによりダイアフィルトレーションして第3の画分を得ること;及び
任意に(optionally)、
(e)補足的なクロマトグラフィの前に、第3の画分をタンジェンシャルフローフィルトレーションにより濃縮すること。 - 前記固相が、ばらの嵩高粒子(loose bulk particles)又はフロースルーデバイス(flow-through device)の形態である、請求項1に記載の方法。
- 前記タンジェンシャルフローフィルトレーションが、単一中空糸の膜若しくは束状の膜などの中空糸膜(hollow-fiber membranes)、又は、単一の膜若しくは積層した若しくは筒状に巻いた膜などの平坦膜からなる群から選択される膜を用いて行われる、請求項1又は請求項2に記載の方法。
- 前記第1のフィルトレーションが、DNAseによるDNA消化を促進するよう調合された緩衝液中で行われる、請求項1~請求項3のいずれか1項に記載の方法。
- タンジェンシャルフローフィルトレーション膜の多孔率が、10kDa~300kDa、30kDa~300kDa、60kDa~300kDa、100kDa~300kDa、200kDa~300kDa、又は250kDa~300kDaの範囲であり、好ましくは分画分子量で100kDa以上の分画分子量(molecular weight cutoff)を有する、請求項3又は請求項4に記載の方法。
- AAVカプシド及び混入DNAを含む調製物が、AAVセロタイプ1、2、3、4、5、6、7、8、9、10、11、又は、2つ以上のセロタイプの特徴を有する組み換えセロタイプなど、他のセロタイプからなる群から選択されるAAV粒子を含む、請求項1~請求項5のいずれか1項に記載の方法。
- ステップ(d)又はステップ(e)の後に、以下の精製ステップの少なくとも1つが用いられる、請求項1~請求項6のいずれか1項に記載の方法:
(i)陽イオン交換クロマトグラフィステップ、又は、
(ii)アフィニティクロマトグラフィステップ、又は、
(iii)陰イオン交換クロマトグラフィステップ。 - 精製ステップ(i)において、ステップ(d)又は(e)の画分が、pH4~6(特に約pH5)で、陽イオン交換クロマトグラフィ材料上にロードされた後に、
陽イオン交換クロマトグラフィ材料を約pH3.5±0.5へ再平衡化すること、
塩勾配を用いた溶出を行うこと、及び
その後、陰イオン交換クロマトグラフィ処理を行うこと、
が行われる、請求項7に記載の方法。 - 精製ステップ(ii)において、ステップ(d)又は(e)の画分が、アフィニティクロマトグラフィ材料上にロードされ、溶出後に、陰イオン交換クロマトグラフィ処理が行われる、請求項7に記載の方法。
- 精製ステップ(iii)において、ステップ(d)又は(e)の画分が、陰イオン交換クロマトグラフィにかけられる、請求項7に記載の方法。
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